LOCUS       sequence-377761-        3622 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Native CcmO fusion with a StrepII tag and PDT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3622)
  AUTHORS   Sakkos JK, Hernandez-Ortiz S, Osteryoung KW, Ducat DC
  TITLE     Orthogonal Degron System for Controlled Protein Degradation in 
            Cyanobacteria.
  JOURNAL   ACS Synth Biol. 2021 Jul 16;10(7):1667-1681. doi: 
            10.1021/acssynbio.1c00035. Epub 2021 Jul 7.
  PUBMED    34232633
REFERENCE   2  (bases 1 to 3622)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00035"; journalName: "ACS Synth Biol"; date: 
            "2021-07-16- 16"; volume: "10"; issue: "7"; pages: "1667-1681"
COMMENT     Sequence Label: pCcmO-StrepII-PDT
FEATURES             Location/Qualifiers
     source          1..3622
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(16..604)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(96..115)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1455..1478
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             1843..2634
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
       61 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
      121 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
      181 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
      241 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
      301 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
      361 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
      421 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
      481 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
      541 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
      601 tcaagaagat cctttgatct atgtcggctt ctcttcccgc ctattctcag cctcgcaatg
      661 caggtgcact aggggtcatt tgtacccgta gttttccagc ggttgtcggc actgcagaca
      721 tgatgctcaa gtcggccgat gtcacattga tcggctatga gaaaacaggc tcgggctttt
      781 gtacagcaat catccggggt ggctatgccg acatcaagct ggctcttgag gctggcgtag
      841 cgacagctcg tcagtttgag cagtacgttt ccagcactat tctgccgcgg cctcaaggca
      901 acctcgaagc cgtgttgccg attagccggc ggctctccca agaagccatg gccacgcgat
      961 cgcatcagaa tgttggcgcg attgggctaa ttgagaccaa tgggttccct gctttggttg
     1021 gagcagccga tgccatgctc aaatcggcta acgtcaagct gatttgttat gagaaaacgg
     1081 gcagcggtct ctgtactgcg atcgtgcaag gcacggtttc taatgtgacc gttgcggtcg
     1141 aagccgggat gtatgccgct gagcggatcg gccagctcaa cgcaatcatg gtcattccca
     1201 gaccgctaga cgacttgatg gacagcttgc ctgagccgca gtcggatagc gaagcagccc
     1261 agccactcca attaccgctg cgggttcgcg aaaaacaacc gctgttggag ctaccggaac
     1321 tcgaacggca gccgatcgcg atcgaagcac cgcgactttt agcagaagag cgacagtctg
     1381 cgttggaatt ggctcaagag acaccgctcg ccgagccctt agagctcccc aatcctcgtg
     1441 atgatcagag cgcttggagc catccgcagt ttgaaaaagc tgctaataaa aacgaagaga
     1501 acacaaacga agttccgacg tttatgctga atgctggcca agcaaaccga cgacgggtct
     1561 aagaaggcac gaacccagtg gacataagcc tgttcggttc gtaagctgta atgcaagtag
     1621 cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca
     1681 gtggcggttt tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca
     1741 tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat
     1801 gttacgcagc agggcagtcg ccctaaaaca aagttaaaca tcatgaggga agcggtgatc
     1861 gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg
     1921 acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt
     1981 gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg
     2041 atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta
     2101 gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa
     2161 ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg
     2221 atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta
     2281 ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta
     2341 aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta
     2401 gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat
     2461 gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa
     2521 gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg
     2581 gaagaatttg tccactacgt gaaaggcgag atcaccaagg tagtcggcaa ataatggaaa
     2641 aagcactgta attcccttgg tttttggctg aaagtttcgg actcagtaga cctaagtaca
     2701 gagtgatgtc aacgccttca agctagacgg gaggcggctt ttgccatggt tcagcgatcg
     2761 ctcctcatct tcaataagca gggcatgagc cagcgttaag caaatcaaat caaatctcgc
     2821 ttctgggctt caataaatgg ttccgattga tgataggttg attcatgagg aatctaaggc
     2881 ttaattctcc acaaaagaat taagcgtccg tcgcaacgga atgctccgct ggacttgcgc
     2941 tgtgggactg cagctttaca ggctccccct gccagaaatc ctgaatcgtc gagcatatct
     3001 gacatatctc tagggagaga cgacatgccc aagacgcaat ctgccgcagg ctataaggcc
     3061 ggggtgaagg actacaaact cacctattac acccccgatt acacccccaa agacactgac
     3121 ctgctggcgg ctttccgctt cagccctcag ccgggtgtcc ctgctgacga agctggtgcg
     3181 gcgatcgcgg ctgaatcttc gaccggtacc tggaccaccg tgtggaccga cttgctgacc
     3241 gacatggatc ggtacaaagg caagtgctac cacatcgagc cggtgcaagg cgaagagaac
     3301 tcctactttg cgttcatcgc ttacccgctc gacctgtttg aagaagggtc ggtcaccaac
     3361 atcctgacct cgatcgtcgg taacgtgttt ggcttcaaag ctatccgttc gctgcgtctg
     3421 gaagacatcc gcttccccgt cgccttggtc aaaaccttcc aaggtcctcc ccacggtatc
     3481 caagtcgagc gcgacctgct gaacaagtac ggccgtccga tgctgggttg cacgatcaaa
     3541 ccaaaactcg gtctgtcggc gaaaaactac ggtcgtgccg tctacgaatg tctgcgcggc
     3601 ggtctggact tcaccaaaga cg
//
