LOCUS       sequence-377782-        2932 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector encoding zebrafish rbm11-202.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2932)
  TITLE     Zebrafish orthologs of chromosome 21 genes
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pTwist-ENTR-rbm11-202
FEATURES             Location/Qualifiers
     source          1..2932
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(563..662)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(719..735)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(719..735)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(732..754)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(907..1495)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(987..1006)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1631..2446)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(1631..2446)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2354..2373
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2438..2457
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     2567..2583
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2567..2583
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     terminator      2584..2630
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2744..2766
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2758..2775
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2759..2775
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2828..2927
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 atgtcgaggc atttatcctt atcaaacaag accatcattg tgggaaacct ccacagatgt
       61 gtgacggagg agattctgtt tgaattgttt cttcaggctg gaccggtgga caaggtgcat
      121 atatccagag atggacagca gtcgccttat gcatgcgttt attataagca tgccgaagct
      181 gttccctatg cagttgaact tctcaatgga atctggctgt atgggcaacc aatcaagtta
      241 cagtgtaata atggaggcca ttatcatagg agtgacgtgt ttgcattcca catggagaat
      301 ggactgataa caaacagtgc tgattctgct tatagggata atatggtttc aaatttaaat
      361 gggtgtccag gtgtggagag tcattctaat ggttggagtg acatggtgta cagcagacct
      421 ctggatgcat gttctccagc tgtgtggtac tgtcctcctc tccagcagtg gatggattct
      481 ggatatcctc cttggacgtc ctgcctgaat gagaatgtgt tgctctgtag gtcagcaggt
      541 gccaaagtga gtcaaattta agacccagct ttcttgtaca aagttggcat tataagaaag
      601 cattgcttat caatttgttg caacgaacag gtcactatca gtcaaaataa aatcattatt
      661 tgaggctagg tggaggctca gtgatgataa gtctgcgatg gtggatgcat gtgtcatggt
      721 catagctgtt tcctgtgtga aattgttatc cgctcagagg gcacaatcct attccgcgct
      781 atccgacaat ctccaagaca ttaggtggag ttcagttcgg cgtatggcat atgtcgctgg
      841 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
      901 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
      961 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     1021 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     1081 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     1141 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     1201 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     1261 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     1321 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     1381 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     1441 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     1501 tcctttgatc ttttctacgg ggtctgacgc tctattcaac aaagccgccg tcccgtcaag
     1561 tcagcgtaaa tgggtagggg gcttcaaatc gtccgctctg ccagtgttac aaccaattaa
     1621 caaattctga ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta ttcatatcag
     1681 gattatcaat accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga
     1741 ggcagttcca taggatggca agatcctggt atcggtctgc gattccgact cgtccaacat
     1801 caatacaacc tattaatttc ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat
     1861 gagtgacgac tgaatccggt gagaatggca aaagcttatg catttctttc cagacttgtt
     1921 caacaggcca gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca
     1981 ttcgtgattg cgcctgagcg agacgaaata cgcgatcgct gttaaaagga caattacaaa
     2041 caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata ttttcacctg
     2101 aatcaggata ttcttctaat acctggaatg ctgttttccc ggggatcgca gtggtgagta
     2161 accatgcatc atcaggagta cggataaaat gcttgatggt cggaagaggc ataaattccg
     2221 tcagccagtt tagtctgacc atctcatctg taacatcatt ggcaacgcta cctttgccat
     2281 gtttcagaaa caactctggc gcatcgggct tcccatacaa tcgatagatt gtcgcacctg
     2341 attgcccgac attatcgcga gcccatttat acccatataa atcagcatcc atgttggaat
     2401 ttaatcgcgg cctcgagcaa gacgtttccc gttgaatatg gctcataaca ccccttgtat
     2461 tactgtttat gtaagcagac agttttattg ttcatgatga tatattttta tcttgtgcaa
     2521 tgtaacatca gagattttga gacacaacgt ggctttcccc cgccgctcta gaactagtgg
     2581 atccaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
     2641 tcgcattata cgagacgtcc aggttgggat acctgaaaca aaacccatcg tacggccaag
     2701 gaagtctcca ataactgtga tccaccacaa gcgccagggt tttcccagtc acgacgttgt
     2761 aaaacgacgg ccagtcatgc ataatccgca cgcatctgga ataaggaagt gccattccgc
     2821 ctgacctcaa ataatgattt tattttgact gatagtgacc tgttcgttgc aacagattga
     2881 tgagcaatgc ttttttataa tgccaacttt gtacaaaaaa gcaggctgca cc
//
