LOCUS       sequence-377784-        3288 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector encoding zebrafish btg3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3288)
  TITLE     Zebrafish orthologs of chromosome 21 genes
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pDONR221-btg3
FEATURES             Location/Qualifiers
     source          1..3288
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1046..1060
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     protein_bind    complement(1413..1512)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1529..1548)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1530..1548)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1553..1569)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1553..1569)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1671..1690)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1682..2491
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1749..1768)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2638..3226
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3127..3146
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggcttc gaaggagata ccaccatgaa gaaagaaatt gcagcggtgg tgtttttcct
      721 gaagcggttg ataaagaaag ctgagaagtt agatgcggac aaggtggacc tgtttgtgga
      781 gcggttaaca gtagcattac aggagaagta taaaggtcac tggtatccgg acaaccccag
      841 caaaggccag gcattcaggt gcatcagagt caaccgcttc cagaaagaag atgctgagtt
      901 gctgagagct tgcgctgaaa gtggagtgca gtacaaagac cttggcctgc ctaaagagct
      961 cacactttgg gttgatcctg gtgaggtgtg ctgtaggtat ggcgagaaga atcatggttt
     1021 cactgtggcc actttctcaa gcgaggacga tgatgataaa gaggatgtga caaagaaagt
     1081 gacgagcgcc gtggagcggg tcacgtcgga ttaccactct ggatcctcct cagatgaaga
     1141 catcagctgt agggagatcc agtatacgac acctcttcac aaccagcctc cttaccaggt
     1201 tgtttatgcc agtgccccag gatggcatcc agtaccaaga aagaaatttg gcccggggaa
     1261 agggcactac cctcagcggc cgcactacat gatccgcccc ccctgtagac ccaactattc
     1321 ctttaaagca cacagctggg tccagcaggg ctaccgcagc aaacccggct actgggccag
     1381 caattcaaac ctactacacc agcttcagtg agacccagct ttcttgtaca aagttggcat
     1441 tataagaaag cattgcttat caatttgttg caacgaacag gtcactatca gtcaaaataa
     1501 aatcattatt tgccatccag ctgatatccc ctatagtgag tcgtattaca tggtcatagc
     1561 tgtttcctgg cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa
     1621 aaatatatca tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt
     1681 tatgagccat attcaacggg aaacgtcgag gccgcgatta aattccaaca tggatgctga
     1741 tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg
     1801 cttgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc
     1861 caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc
     1921 gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc
     1981 cggaaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga
     2041 tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa
     2101 cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga
     2161 tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat
     2221 gcataaactt ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga
     2281 taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat
     2341 cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc
     2401 attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca
     2461 gtttcatttg atgctcgatg agtttttcta atcagaattg gttaattggt tgtaacactg
     2521 gcagagcatt acgctgactt gacgggacgg cgcaagctca tgaccaaaat cccttaacgt
     2581 gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     2641 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     2701 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     2761 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     2821 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     2881 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     2941 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     3001 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     3061 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     3121 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     3181 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     3241 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgtt
//
