LOCUS       sequence-377786-        3191 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lux synthase (3OC6-HSL).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Kokarakis EJ, Rillema R, Ducat DC, Sakkos JK
  TITLE     Developing Cyanobacterial Quorum Sensing Toolkits: Toward 
            Interspecies Coordination in Mixed Autotroph/Heterotroph 
            Communities.
  JOURNAL   ACS Synth Biol. 2023 Jan 20;12(1):265-276. doi: 
            10.1021/acssynbio.2c00527. Epub 2022 Dec 27.
  PUBMED    36573789
REFERENCE   2  (bases 1 to 3191)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.2c00527"; journalName: "ACS Synth Biol"; date: 
            "2023-01-20- 20"; volume: "12"; issue: "1"; pages: "265-276"
COMMENT     Sequence Label: pBbA2C_Ptet::luxI
FEATURES             Location/Qualifiers
     source          1..3191
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     356..375
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(442..544)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             complement(574..1200)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     1105..1124
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     promoter        1216..1271
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    1252..1270
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      1923..1994
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2010..2037
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(2065..2082)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2199..2744)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(2279..2298)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2858..2952)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(2973..3191,1..441))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 ggctgagacg aaaaacatat tctcaataaa ccctttaggg aaataggcca ggttttcacc
       61 gtaacacgcc acatcttgcg aatatatgtg tagaaactgc cggaaatcgt cgtggtattc
      121 actccagagc gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac aagggtgaac
      181 actatcccat atcaccagct caccgtcttt cattgccata cgaaattccg gatgagcatt
      241 catcaggcgg gcaagaatgt gaataaaggc cggataaaac ttgtgcttat ttttctttac
      301 ggtctttaaa aaggccgtaa tatccagctg aacggtctgg ttataggtac attgagcaac
      361 tgactgaaat gcctcaaaat gttctttacg atgccattgg gatatatcaa cggtggtata
      421 tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg ataactcaaa
      481 aaatacgccc ggtagtgatc ttatttcatt atggtgaaag ttggaacctc ttacgtgccg
      541 atcaacgtct cattttcgcc agatatcgac gtcttaagac ccactttcac atttaagttg
      601 tttttctaat ccgcatatga tcaattcaag gccgaataag aaggctggct ctgcaccttg
      661 gtgatcaaat aattcgatag cttgtcgtaa taatggcggc atactatcag tagtaggtgt
      721 ttccctttct tctttagcga cttgatgctc ttgatcttcc aatacgcaac ctaaagtaaa
      781 atgccccaca gcgctgagtg catataatgc attctctagt gaaaaacctt gttggcataa
      841 aaaggctaat tgattttcga gagtttcata ctgtttttct gtaggccgtg tacctaaatg
      901 tacttttgct ccatcgcgat gacttagtaa agcacatcta aaacttttag cgttattacg
      961 taaaaaatct tgccagcttt ccccttctaa agggcaaaag tgagtatggt gcctatctaa
     1021 catctcaatg gctaaggcgt cgagcaaagc ccgcttattt tttacatgcc aatacaatgt
     1081 aggctgctct acacctagct tctgggcgag tttacgggtt gttaaacctt cgattccgac
     1141 ctcattaagc agctctaatg cgctgttaat cactttactt ttatctaatc tagacatcat
     1201 taattcctaa tttttgttga cactctatcg ttgatagagt tattttacca ctccctatca
     1261 gtgatagaga aaagaattca aaagatcttt taagaaggag atatacatat gactataatg
     1321 ataaaaaaat cggatttttt ggcaattcca tcggaggagt ataaaggtat tctaagtctt
     1381 cgttatcaag tgtttaagca aagacttgag tgggacttag ttgtagaaaa taaccttgaa
     1441 tcagatgagt atgataactc aaatgcagaa tatatttatg cttgtgatga tactgaaaat
     1501 gtaagtggat gctggcgttt attacctaca acaggtgatt atatgctgaa aagtgttttt
     1561 cctgaattgc ttggtcaaca gagtgctccc aaagatccta atatagtcga attaagtcgt
     1621 tttgctgtag gtaaaaatag ctcaaagata aataactctg ctagtgaaat tacaatgaaa
     1681 ctatttgaag ctatatataa acacgctgtt agtcaaggta ttacagaata tgtaacagta
     1741 acatcaacag caatagagcg atttttaaag cgtattaaag ttccttgtca tcgtattgga
     1801 gacaaagaaa ttcatgtatt aggtgatact aaatcggttg tattgtctat gcctattaat
     1861 gaacagttta aaaaagcagt cttaaattaa ggatccaaac tcgagtaagg atctccaggc
     1921 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
     1981 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
     2041 atacctaggg atatattccg cttcctcgct cactgactcg ctacgctcgg tcgttcgact
     2101 gcggcgagcg gaaatggctt acgaacgggg cggagatttc ctggaagatg ccaggaagat
     2161 acttaacagg gaagtgagag ggccgcggca aagccgtttt tccataggct ccgcccccct
     2221 gacaagcatc acgaaatctg acgctcaaat cagtggtggc gaaacccgac aggactataa
     2281 agataccagg cgtttccccc tggcggctcc ctcgtgcgct ctcctgttcc tgcctttcgg
     2341 tttaccggtg tcattccgct gttatggccg cgtttgtctc attccacgcc tgacactcag
     2401 ttccgggtag gcagttcgct ccaagctgga ctgtatgcac gaaccccccg ttcagtccga
     2461 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggaaagac atgcaaaagc
     2521 accactggca gcagccactg gtaattgatt tagaggagtt agtcttgaag tcatgcgccg
     2581 gttaaggcta aactgaaagg acaagttttg gtgactgcgc tcctccaagc cagttacctc
     2641 ggttcaaaga gttggtagct cagagaacct tcgaaaaacc gccctgcaag gcggtttttt
     2701 cgttttcaga gcaagagatt acgcgcagac caaaacgatc tcaagaagat catcttatta
     2761 atcagataaa atatttctag atttcagtgc aatttatctc ttcaaatgta gcacctgaag
     2821 tcagccccat acgatataag ttgttactag tgcttggatt ctcaccaata aaaaacgccc
     2881 ggcggcaacc gagcgttctg aacaaatcca gatggagttc tgaggtcatt actggatcta
     2941 tcaacaggag tccaagcgag ctcgatatca aattacgccc cgccctgcca ctcatcgcag
     3001 tactgttgta attcattaag cattctgccg acatggaagc catcacaaac ggcatgatga
     3061 acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatggtg
     3121 aaaacggggg cgaagaagtt gtccatattg gccacgttta aatcaaaact ggtgaaactc
     3181 acccagggat t
//
