LOCUS       sequence-377789-        3218 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Las synthase (3OC12-HSL).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Kokarakis EJ, Rillema R, Ducat DC, Sakkos JK
  TITLE     Developing Cyanobacterial Quorum Sensing Toolkits: Toward 
            Interspecies Coordination in Mixed Autotroph/Heterotroph 
            Communities.
  JOURNAL   ACS Synth Biol. 2023 Jan 20;12(1):265-276. doi: 
            10.1021/acssynbio.2c00527. Epub 2022 Dec 27.
  PUBMED    36573789
REFERENCE   2  (bases 1 to 3218)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.2c00527"; journalName: "ACS Synth Biol"; date: 
            "2023-01-20- 20"; volume: "12"; issue: "1"; pages: "265-276"
COMMENT     Sequence Label: pBbA2C_Ptet::lasI
FEATURES             Location/Qualifiers
     source          1..3218
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     347..366
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(433..535)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             complement(565..1191)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     1096..1115
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     promoter        1207..1262
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    1243..1261
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      1941..2012
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2028..2055
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(2083..2100)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2217..2762)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(2297..2316)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2876..2970)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(2991..3218,1..432))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 gaaaaacata ttctcaataa accctttagg gaaataggcc aggttttcac cgtaacacgc
       61 cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt cactccagag
      121 cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca
      181 tatcaccagc tcaccgtctt tcattgccat acgaaattcc ggatgagcat tcatcaggcg
      241 ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa
      301 aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa
      361 tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat
      421 ttttttctcc attttagctt ccttagctcc tgaaaatctc gataactcaa aaaatacgcc
      481 cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc gatcaacgtc
      541 tcattttcgc cagatatcga cgtcttaaga cccactttca catttaagtt gtttttctaa
      601 tccgcatatg atcaattcaa ggccgaataa gaaggctggc tctgcacctt ggtgatcaaa
      661 taattcgata gcttgtcgta ataatggcgg catactatca gtagtaggtg tttccctttc
      721 ttctttagcg acttgatgct cttgatcttc caatacgcaa cctaaagtaa aatgccccac
      781 agcgctgagt gcatataatg cattctctag tgaaaaacct tgttggcata aaaaggctaa
      841 ttgattttcg agagtttcat actgtttttc tgtaggccgt gtacctaaat gtacttttgc
      901 tccatcgcga tgacttagta aagcacatct aaaactttta gcgttattac gtaaaaaatc
      961 ttgccagctt tccccttcta aagggcaaaa gtgagtatgg tgcctatcta acatctcaat
     1021 ggctaaggcg tcgagcaaag cccgcttatt ttttacatgc caatacaatg taggctgctc
     1081 tacacctagc ttctgggcga gtttacgggt tgttaaacct tcgattccga cctcattaag
     1141 cagctctaat gcgctgttaa tcactttact tttatctaat ctagacatca ttaattccta
     1201 atttttgttg acactctatc gttgatagag ttattttacc actccctatc agtgatagag
     1261 aaaagaattc aaaagatctt ttaagaagga gatatacata tgatcgttca gatcggtcgt
     1321 cgtgaagagt tcgacaaaaa actgctgggt gaaatgcaca aactgcgtgc tcaggttttc
     1381 aaagaacgta aaggttggga cgtttccgtt atcgacgaaa tggaaatcga cggttacgac
     1441 gctctgtccc cgtactacat gctgatccag gaagacaccc cggaagctca ggttttcggt
     1501 tgctggcgta tcttcgacac caccggtccg tacatgctga aaaacacctt cccggaactg
     1561 ctgcacggta aagaagctcc gtgctccccg cacatctggg aactgtcccg tttcgctatc
     1621 aactccggtc agaaaggttc cctgggtttc tccgactgca ccctggaagc tatgcgtgct
     1681 ctggctcgtt actccttgca gaacgacatc cagaccctgg ttaccgttac caccgttggt
     1741 gttgaaaaaa tgatgatccg tgctggtctg gacgtttccc gtttcggtcc gcacctgaaa
     1801 atcggtatcg aacgtgctgt tgctctgcgt atcgaactga acgctaaaac ccagatcgct
     1861 ctgtacggtg gtgttctggt tgaacagcgt ctggctgttt cctaataagg atccaaactc
     1921 gagtaaggat ctccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
     1981 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
     2041 gtgggccttt ctgcgtttat acctagggat atattccgct tcctcgctca ctgactcgct
     2101 acgctcggtc gttcgactgc ggcgagcgga aatggcttac gaacggggcg gagatttcct
     2161 ggaagatgcc aggaagatac ttaacaggga agtgagaggg ccgcggcaaa gccgtttttc
     2221 cataggctcc gcccccctga caagcatcac gaaatctgac gctcaaatca gtggtggcga
     2281 aacccgacag gactataaag ataccaggcg tttccccctg gcggctccct cgtgcgctct
     2341 cctgttcctg cctttcggtt taccggtgtc attccgctgt tatggccgcg tttgtctcat
     2401 tccacgcctg acactcagtt ccgggtaggc agttcgctcc aagctggact gtatgcacga
     2461 accccccgtt cagtccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     2521 ggaaagacat gcaaaagcac cactggcagc agccactggt aattgattta gaggagttag
     2581 tcttgaagtc atgcgccggt taaggctaaa ctgaaaggac aagttttggt gactgcgctc
     2641 ctccaagcca gttacctcgg ttcaaagagt tggtagctca gagaaccttc gaaaaaccgc
     2701 cctgcaaggc ggttttttcg ttttcagagc aagagattac gcgcagacca aaacgatctc
     2761 aagaagatca tcttattaat cagataaaat atttctagat ttcagtgcaa tttatctctt
     2821 caaatgtagc acctgaagtc agccccatac gatataagtt gttactagtg cttggattct
     2881 caccaataaa aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg
     2941 aggtcattac tggatctatc aacaggagtc caagcgagct cgatatcaaa ttacgccccg
     3001 ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca
     3061 tcacaaacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta
     3121 taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa
     3181 tcaaaactgg tgaaactcac ccagggattg gctgagac
//
