LOCUS       sequence-377797-        3452 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector encoding zebrafish chodl.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3452)
  TITLE     Zebrafish orthologs of chromosome 21 genes
REFERENCE   2  (bases 1 to 3452)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pDONR221-chodl
FEATURES             Location/Qualifiers
     source          1..3452
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1577..1676)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1693..1712)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1694..1712)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1717..1733)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1717..1733)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1835..1854)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1846..2655
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1913..1932)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2802..3390
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3291..3310
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt ttacaaaaaa
      661 gcaggcttcg aaggagatac caccatgcgc gcgacactca ggatcctttg tgctttgact
      721 tttctggtct cgtgcagccg tggcgcgaga gttgtgagcg gtcagacagt gtgtcagggc
      781 aaccctgaac acccgtgcta taagattgct tacttcaaag acgtgtcgag tcgtgttgcg
      841 ttctgggagg ctctgcaggc atgtgaaatg gacggcggtt cgctgctgag cattgagaac
      901 acagccgagc agaagcacat tgaacacctt ctgcgagagc tcagcgtctc ctcctcgact
      961 ggtcctgcca gcattacaga cggagacttc tggatcggcc tgacccgtga ggagggtgat
     1021 aatgcacagg agcccggcgc cttcgcctcc tgtcccaatc tgtacaggtg gactgatgga
     1081 agtgtctcgc tgttcagaaa ctggtatgct gatgaaccat cctgcggagg agaagcgtgt
     1141 gtggttatgt atcaccagcc tacagctctg gccgggcctg gaggaccgta tctctaccag
     1201 tggaacgacg acaggtgcaa tatgaagcac aatttcatct gcaagtatga accagaaatt
     1261 catctggtta aagtgcagag tgataggcct ggagggcatg atgttgatct ttccactgaa
     1321 gataaagagg acaggagaac accacccact gatgaggatg agagccctcg acttataata
     1381 gctgggcctt caagtatgct gctaatctac gtgattatcc ctaccatccc tctcctcttg
     1441 ctgatcctgg tggcttcagg gacgtgctgt ttccagatgt tgagcaagag caaaccacgg
     1501 accaaaacta gcgtcaacca atcaactctc tggatctcca agacgccaaa gatagacagt
     1561 ggcatggagg tctaagaccc agctttcttg tacaaagttg gcattataag aaagcattgc
     1621 ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgccat
     1681 ccagctgata tcccctatag tgagtcgtat tacatggtca tagctgtttc ctggcagctc
     1741 tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatga
     1801 acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcaa
     1861 cgggaaacgt cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa
     1921 tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta tgggaagccc
     1981 gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat
     2041 gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt
     2101 atccgtactc ctgatgatgc atggttactc accactgcga tccccggaaa aacagcattc
     2161 caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc
     2221 ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt
     2281 cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat
     2341 gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa acttttgcca
     2401 ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac
     2461 gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag
     2521 gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt
     2581 tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc
     2641 gatgagtttt tctaatcaga attggttaat tggttgtaac actggcagag cattacgctg
     2701 acttgacggg acggcgcaag ctcatgacca aaatccctta acgtgagtta cgcgtcgttc
     2761 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     2821 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     2881 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     2941 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     3001 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     3061 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     3121 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     3181 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     3241 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     3301 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     3361 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     3421 ctggcctttt gctggccttt tgctcacatg tt
//
