LOCUS       sequence-377799-        3384 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector encoding zebrafish olig2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3384)
  TITLE     Zebrafish orthologs of chromosome 21 genes
REFERENCE   2  (bases 1 to 3384)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pDONR221-olig2
FEATURES             Location/Qualifiers
     source          1..3384
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1509..1608)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1625..1644)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1626..1644)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1649..1665)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1649..1665)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1767..1786)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1778..2587
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1845..1864)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2734..3322
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3223..3242
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggcttc gaaggagata ccaccatgga ctctgacacg agccgagtgt ccagcagacc
      721 ttcttctcct gaaggcgatg atctttttct gtccgccgtc aagaagtccg tgggtttctc
      781 cggcgccgtc tcctcaacgc agagcgattc tccaccggag ttaagaggtg cagatatgcg
      841 gggcatctcc agcgctgagg aggacgcaat ggctctaaag atgctctcca aaaaggaccg
      901 taaacttctc tccgagaatg agctgcagag catgcgccta aagatcaaca gccgcgagag
      961 gaagcgcatg cacgacctta acatcgccat ggacgggctc cgggaggtca tgccctacgc
     1021 tcacgggccg tctgtgcgca agctctctaa aatagccact ctgctgctcg cgcgcaacta
     1081 catccttatg ctgagcaact cgctagagga gatgaagcgg ctcgtgagcg agatctacgg
     1141 cggagggagc ggggcccatc acgccggttt ccatccgtcc agttgtggca ctcttacgca
     1201 cgcggcggcg gctccgttac cgggtcaccc cgctgctgtc tcgcattcct ctcacccggt
     1261 gcaccatcct ctgcttccac cagcagtctc caccgcagca tctctttccg ggacggggct
     1321 ttcagccgtc aggccgcatc acggactcct caaagccccg tcagccggcg ctgggcctct
     1381 gggcgcgagt ttccagcact ggggtgcggg gatcccttgt ccgtgtagca tgtgccaggt
     1441 gcctccgtct cacgttcccg gtatgagcgc cgtcagcatg ccacggctga ccagtgactc
     1501 aaaataagac ccagctttct tgtacaaagt tggcattata agaaagcatt gcttatcaat
     1561 ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgcc atccagctga
     1621 tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt
     1681 gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat gaacaataaa
     1741 actgtctgct tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac
     1801 gtcgaggccg cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg
     1861 cgataatgtc gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc
     1921 agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt
     1981 cagactaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac
     2041 tcctgatgat gcatggttac tcaccactgc gatccccgga aaaacagcat tccaggtatt
     2101 agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg
     2161 gttgcattcg attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc
     2221 tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg
     2281 taatggctgg cctgttgaac aagtctggaa agaaatgcat aaacttttgc cattctcacc
     2341 ggattcagtc gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa
     2401 attaataggt tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc
     2461 catcctatgg aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa
     2521 atatggtatt gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt
     2581 tttctaatca gaattggtta attggttgta acactggcag agcattacgc tgacttgacg
     2641 ggacggcgca agctcatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc
     2701 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     2761 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     2821 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     2881 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     2941 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     3001 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     3061 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     3121 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     3181 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     3241 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     3301 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     3361 ttgctggcct tttgctcaca tgtt
//
