LOCUS       sequence-377800-        5724 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector encoding zebrafish nrip1b.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5724)
  TITLE     Zebrafish orthologs of chromosome 21 genes
REFERENCE   2  (bases 1 to 5724)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pDONR221-nrip1b
FEATURES             Location/Qualifiers
     source          1..5724
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(200..299)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(316..335)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(317..335)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(340..356)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(340..356)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(458..477)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             469..1278
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(536..555)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1425..2013
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1914..1933
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2167..2184
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2343..2370)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2462..2548)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2597..2619
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2611..2628
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2612..2628
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2644..2743
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 cctttaacca ggtctaatcc gattctttac tacatgcttc agcggggaaa tggtcagctc
       61 agacctgaga tgcgggatca gatgcagtcg ccccactgcg tcatgggtga ctatgagcac
      121 aggataaact ctccggttca cagacagttt cacagccaga gagaagacag cctaaatggc
      181 tctgtggaaa aatggtagga cccagctttc ttgtacaaag ttggcattat aagaaagcat
      241 tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat cattatttgc
      301 catccagctg atatccccta tagtgagtcg tattacatgg tcatagctgt ttcctggcag
      361 ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca
      421 tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat gagccatatt
      481 caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat
      541 aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag
      601 cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca
      661 gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat
      721 tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg aaaaacagca
      781 ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg
      841 ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta
      901 tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt
      961 gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca taaacttttg
     1021 ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt
     1081 gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac
     1141 caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg
     1201 ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg
     1261 ctcgatgagt ttttctaatc agaattggtt aattggttgt aacactggca gagcattacg
     1321 ctgacttgac gggacggcgc aagctcatga ccaaaatccc ttaacgtgag ttacgcgtcg
     1381 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     1441 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     1501 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     1561 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     1621 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     1681 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     1741 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     1801 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     1861 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     1921 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     1981 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     2041 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     2101 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     2161 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     2221 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     2281 ggcagtgagc gcaacgcaat taatacgcgt accgctagcc aggaagagtt tgtagaaacg
     2341 caaaaaggcc atccgtcagg atggccttct gcttagtttg atgcctggca gtttatggcg
     2401 ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg
     2461 gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt
     2521 cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct cgcgttaacg
     2581 ctagcatgga tgttttccca gtcacgacgt tgtaaaacga cggccagtct taagctcggg
     2641 ccccaaataa tgattttatt ttgactgata gtgacctgtt cgttgcaaca aattgatgag
     2701 caatgctttt ttataatgcc aactttgtac aaaaaagcag gcttcgaagg agataccacc
     2761 atgactcatg gggtggagtc tggccctgaa actcaccagg attctgctgc tttaacatat
     2821 ctggaaggtt tgctaatgca tccggtcaac actgggcctg ctgccactgc tacacaaaga
     2881 tcagaacctg tccacaacaa tgaagagaat gccaacagcg taaccagggc atttcagctg
     2941 cacaaccatg gcgccctttc accagaaaag ggtagcccgc ctagtgcaac ctcgcagcac
     3001 cttaaaaaag ccaggctgct tcgttcagga gcatggagtg aagacagcca gaaacaatca
     3061 agcactggag tggaggtaaa tgggcagaga agggagcact acaaaggcgg cctggatggc
     3121 tctgggcagg gagagagcac cttgctggct tcgctgctgc agtccttcag ctctcggctg
     3181 cagagtgtcg ccttgtcaca gcacatttct caaagccaca aacagctcga cagagagagc
     3241 agtgaaagtg cgcctgttga tcctaaggaa acctaccaga gttacggcac agcatcaggc
     3301 cgcctgaaaa gcctcatgag gaaaagcaag catcagaacc ataacactgt gccttattgc
     3361 cgacagagca accagaaccg aggctctgat tctcctcggt cctcacaaaa ctctgcccag
     3421 cccacaagct ctgaatcaat gtcttgcaca gaacgactca aggctgttgc cagtatggtt
     3481 cggacaagat ccagtccggc accttcaccc aaacccagtg tcgcctgcag tcagctggca
     3541 ttgttgctgt ccagcgaggc tcatctacag caatactcta gagagcaggc tttaaaagca
     3601 caattaacca gtcgatcagc aagtgagagg ctagctgcta tggctactca acaaacacag
     3661 gataacagac ctcccagcat gggacagcca acaaccaccc cagacatgct aagctcctta
     3721 aatgctcaaa acggaacaat ccccccatta gtggttaact ccagcaaaag cagcccttcc
     3781 ctttcctcac cctccacgag accccctaaa gagagacggc cttttgacag gcacggtcga
     3841 ccgccacaga actgcagcag tctgttgctt ctacttctta acaatcacaa ctcccaacag
     3901 cagctcacca gaaacggaca cctagaggaa gactgcggca tcctcccaag ctgtgcctct
     3961 tccttgcagt ctgacagcga gtactccaac ccggataaca gcctgaccaa agacagcagt
     4021 gatgctgaga gcttgtccag ctgttccccc atcgaccttt ccatgaaaag cagaacgtct
     4081 tgccaaaaat ttgagccctg ctattccttg ccccccttga tggataagct cacagagtct
     4141 ctcgtaaaca aatggaagcc ggaaacttct gtgccaaagg tccacgaggc cgtagatctg
     4201 gataccagcc cggacataaa atcccaagat aaggtcactc taatgcagtt actgctggac
     4261 cgcagaaata atgagaaggt aaacaaaagt tcagatcatc ctggtttgag gtctgattca
     4321 gtaatcagct gcttgactgc aggctcactt aaacgcattg gcacaccaga ggactccagg
     4381 acacagagcc ctcaggaccg gcaagtagct agcttcagct cagtctcccc ttcatactcc
     4441 ttcccctcac ccgttgccca gtccagtcct ttagatctct gcaagtctaa agctcactca
     4501 agtgaaaaga tggagccgcc gttcagtgcc agcaagctac tgcagaattt ggcccagagt
     4561 ggtataaaga acttgtcacc ttctccccct cctttacacc ctcacaagtt gacaagcaaa
     4621 aggcaaagtc cagtacttga gcttggaaag ccccaggcct tcttgaatag acttgtcact
     4681 ccagccaaac agaacagtcc ccctgtattg gacgaaggtt cccccaaatg tgagcaatca
     4741 ccctcttcat cattgcagat agaaaacctt cttgagaagc gcacagtact gcagcttcta
     4801 ctgggaacag cctcccaaaa ggaaaaaaac agtggacaca gagtcttaga agttacctca
     4861 agggggatgg agaaggcaag ggtgggctct gctaagtgtg acagctcaaa tggacctcct
     4921 ctggacccta acatcaaaaa ggagccagta gaagagaatc ttcaatcaga ccgctttaat
     4981 gatggggtgc accacctgag tagatcatca caacactccc ccattgctga agtacaggac
     5041 acaatcaaat ctgagctatg tcccacagaa acagctgcta aatttggact tctcagccag
     5101 cttttgaaac agcagaatgc catataccat tcgagcccac acacaaggct tgtcaggaac
     5161 actgtaaaag aggagccaat ggattttcac agtccaattc cgaagaaaag aaaactgtgc
     5221 attgagttag ctgagcgtct aagtagtgaa ctctgtcaac cgcctgaaga tgtgactagc
     5281 gacaatccag catctggaac acctgaattg aaaggtagag ggctggaaaa actaaaggag
     5341 gatgaaccac tagggagtcc agggaacaaa actcctctct ccagagaaag ccaagggttt
     5401 aatgttctca agcaacttct cctgtcagaa aactgtctga aggagctatc ccaacccaga
     5461 ggtaccccaa gtcccttcat ccctcaggca aactgtgcag ccaatgggaa cttgacccag
     5521 tctggtttcc gtcaagagtt gtcaaactta ccatggtatc cctattcttt cagttctggc
     5581 cccaaaacta cgcccacacc cgttgatacc ccagtttggg ctaatgtctc tcccaaagcc
     5641 agccctaaac cagttaaaaa agaacctgag gggtctcctg aagaaacttt tagcagggag
     5701 gagagatcta gtccagactc gccc
//
