LOCUS       sequence-377803-        2842 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector encoding zebrafish rbm11-203.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2842)
  TITLE     Zebrafish orthologs of chromosome 21 genes
REFERENCE   2  (bases 1 to 2842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pTwist-ENTR-rbm11-203
FEATURES             Location/Qualifiers
     source          1..2842
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(473..572)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(629..645)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(629..645)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(642..664)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(817..1405)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(897..916)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1541..2356)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(1541..2356)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2264..2283
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2348..2367
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     2477..2493
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2477..2493
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     terminator      2494..2540
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2654..2676
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2668..2685
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2669..2685
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2738..2837
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 atgtcgaggc atttatcctt atcaaacaag accatcattg tgggaaacct ccacagatgt
       61 gtgacggagg agattctgtt tgaattgttt cttcaggctg gaccggtgga caaggtgcat
      121 atatccagag atggacagca gtcgccttat gcatgcgttt attataagca tgccgaagct
      181 gttccctatg cagttgaact tctcaatgga atctggctgt atgggcaacc aatcaagtta
      241 cagtgtaata atggaggcca ttatcatagg agtgacgtgt ttgcattcca catggagaat
      301 ggactgataa caaacagtgc tgattctgct tatagggata atatggtttc aaatttaaat
      361 gggtgtccag gtgtggagag tcattctaat ggttggagtg acatggtaag taagaacaat
      421 aacaagaaga agacgctttt tcttcatatc agcaaattga ccaacagata agacccagct
      481 ttcttgtaca aagttggcat tataagaaag cattgcttat caatttgttg caacgaacag
      541 gtcactatca gtcaaaataa aatcattatt tgaggctagg tggaggctca gtgatgataa
      601 gtctgcgatg gtggatgcat gtgtcatggt catagctgtt tcctgtgtga aattgttatc
      661 cgctcagagg gcacaatcct attccgcgct atccgacaat ctccaagaca ttaggtggag
      721 ttcagttcgg cgtatggcat atgtcgctgg aaagaacatg tgagcaaaag gccagcaaaa
      781 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
      841 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
      901 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
      961 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     1021 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     1081 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     1141 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     1201 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     1261 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     1321 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     1381 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     1441 tctattcaac aaagccgccg tcccgtcaag tcagcgtaaa tgggtagggg gcttcaaatc
     1501 gtccgctctg ccagtgttac aaccaattaa caaattctga ttagaaaaac tcatcgagca
     1561 tcaaatgaaa ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc
     1621 gtttctgtaa tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt
     1681 atcggtctgc gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa
     1741 aaataaggtt atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca
     1801 aaagcttatg catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa
     1861 aatcactcgc atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg agacgaaata
     1921 cgcgatcgct gttaaaagga caattacaaa caggaatcga atgcaaccgg cgcaggaaca
     1981 ctgccagcgc atcaacaata ttttcacctg aatcaggata ttcttctaat acctggaatg
     2041 ctgttttccc ggggatcgca gtggtgagta accatgcatc atcaggagta cggataaaat
     2101 gcttgatggt cggaagaggc ataaattccg tcagccagtt tagtctgacc atctcatctg
     2161 taacatcatt ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct
     2221 tcccatacaa tcgatagatt gtcgcacctg attgcccgac attatcgcga gcccatttat
     2281 acccatataa atcagcatcc atgttggaat ttaatcgcgg cctcgagcaa gacgtttccc
     2341 gttgaatatg gctcataaca ccccttgtat tactgtttat gtaagcagac agttttattg
     2401 ttcatgatga tatattttta tcttgtgcaa tgtaacatca gagattttga gacacaacgt
     2461 ggctttcccc cgccgctcta gaactagtgg atccaaataa aacgaaaggc tcagtcgaaa
     2521 gactgggcct ttcgttttat ctgttgtttg tcgcattata cgagacgtcc aggttgggat
     2581 acctgaaaca aaacccatcg tacggccaag gaagtctcca ataactgtga tccaccacaa
     2641 gcgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtcatgc ataatccgca
     2701 cgcatctgga ataaggaagt gccattccgc ctgacctcaa ataatgattt tattttgact
     2761 gatagtgacc tgttcgttgc aacagattga tgagcaatgc ttttttataa tgccaacttt
     2821 gtacaaaaaa gcaggctgca cc
//
