LOCUS       sequence-377804-        3198 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector encoding human LINC00114-202.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3198)
  TITLE     Zebrafish orthologs of chromosome 21 genes
REFERENCE   2  (bases 1 to 3198)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pTwist-ENTR-ENST00000429621.1
FEATURES             Location/Qualifiers
     source          1..3198
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(829..928)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(985..1001)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(985..1001)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(998..1020)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(1173..1761)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1253..1272)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1897..2712)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(1897..2712)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2620..2639
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2704..2723
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     2833..2849
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2833..2849
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     terminator      2850..2896
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3010..3032
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3024..3041
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3025..3041
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3094..3193
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 tttcctgagg cctcctccgg agtggagcag atgctgccat gcttcctgtg cagcctgtgg
       61 aacgggtctc attctgtctc ccaggctgga gtgcaatggc acaatcatgg ctcactgcag
      121 cttcaacctc ctgggctcaa gcaatcctcc cacctcagcc tccagagcaa ctgggactac
      181 agggacttta agaagctgct gaagaaccca agaggaaggt gggagctgtt gatggtgtgg
      241 aattagaggc ctgatggagt ggagagcttt cctcctcatc acctgggtca ggaccatgga
      301 gctccttgag ccttgagatt ccaggcccag gagcacatca tcattgtgct tcttggggcc
      361 caggcgcatc tcctaagaac ttgttcttag acatgttgtg ctcaaacttg tggcatcata
      421 gcagtaatag tttacaatat acctacagtt tcctgggcag atcatcgtgt gcagaccggc
      481 tgcagagaga agggaactgt ttcactttca tcttcctggg ccatttccag atatgaattg
      541 cagagacata tttcaatata tcctcaccca aatatgcttt agaagtacat ttcttttaat
      601 gtaataagat ttatgtcctt tgtgtttgtt tagagggtaa atctggattt gtatttttag
      661 aagttacttt caccaaagaa attcgaaaga aatagtgttt cagaaaaatc aaaacaagcc
      721 atagaaatct aatatgtttc agatactata ttgggctgca tgaagactaa atccataata
      781 tgtatgaata atgtattcta aagtaaatat catattttgt caaattagac ccagctttct
      841 tgtacaaagt tggcattata agaaagcatt gcttatcaat ttgttgcaac gaacaggtca
      901 ctatcagtca aaataaaatc attatttgag gctaggtgga ggctcagtga tgataagtct
      961 gcgatggtgg atgcatgtgt catggtcata gctgtttcct gtgtgaaatt gttatccgct
     1021 cagagggcac aatcctattc cgcgctatcc gacaatctcc aagacattag gtggagttca
     1081 gttcggcgta tggcatatgt cgctggaaag aacatgtgag caaaaggcca gcaaaaggcc
     1141 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     1201 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     1261 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     1321 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     1381 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     1441 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     1501 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     1561 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     1621 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     1681 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     1741 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcta
     1801 ttcaacaaag ccgccgtccc gtcaagtcag cgtaaatggg tagggggctt caaatcgtcc
     1861 gctctgccag tgttacaacc aattaacaaa ttctgattag aaaaactcat cgagcatcaa
     1921 atgaaactgc aatttattca tatcaggatt atcaatacca tatttttgaa aaagccgttt
     1981 ctgtaatgaa ggagaaaact caccgaggca gttccatagg atggcaagat cctggtatcg
     2041 gtctgcgatt ccgactcgtc caacatcaat acaacctatt aatttcccct cgtcaaaaat
     2101 aaggttatca agtgagaaat caccatgagt gacgactgaa tccggtgaga atggcaaaag
     2161 cttatgcatt tctttccaga cttgttcaac aggccagcca ttacgctcgt catcaaaatc
     2221 actcgcatca accaaaccgt tattcattcg tgattgcgcc tgagcgagac gaaatacgcg
     2281 atcgctgtta aaaggacaat tacaaacagg aatcgaatgc aaccggcgca ggaacactgc
     2341 cagcgcatca acaatatttt cacctgaatc aggatattct tctaatacct ggaatgctgt
     2401 tttcccgggg atcgcagtgg tgagtaacca tgcatcatca ggagtacgga taaaatgctt
     2461 gatggtcgga agaggcataa attccgtcag ccagtttagt ctgaccatct catctgtaac
     2521 atcattggca acgctacctt tgccatgttt cagaaacaac tctggcgcat cgggcttccc
     2581 atacaatcga tagattgtcg cacctgattg cccgacatta tcgcgagccc atttataccc
     2641 atataaatca gcatccatgt tggaatttaa tcgcggcctc gagcaagacg tttcccgttg
     2701 aatatggctc ataacacccc ttgtattact gtttatgtaa gcagacagtt ttattgttca
     2761 tgatgatata tttttatctt gtgcaatgta acatcagaga ttttgagaca caacgtggct
     2821 ttcccccgcc gctctagaac tagtggatcc aaataaaacg aaaggctcag tcgaaagact
     2881 gggcctttcg ttttatctgt tgtttgtcgc attatacgag acgtccaggt tgggatacct
     2941 gaaacaaaac ccatcgtacg gccaaggaag tctccaataa ctgtgatcca ccacaagcgc
     3001 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tcatgcataa tccgcacgca
     3061 tctggaataa ggaagtgcca ttccgcctga cctcaaataa tgattttatt ttgactgata
     3121 gtgacctgtt cgttgcaaca gattgatgag caatgctttt ttataatgcc aactttgtac
     3181 aaaaaagcag gctgcacc
//
