LOCUS       sequence-377805-        3010 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector encoding zebrafish NP_001373296.1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3010)
  TITLE     Zebrafish orthologs of chromosome 21 genes
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pTwist-ENTR-NP_001373296.1
FEATURES             Location/Qualifiers
     source          1..3010
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(641..740)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(797..813)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(797..813)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(810..832)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(985..1573)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1065..1084)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1709..2524)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(1709..2524)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2432..2451
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2516..2535
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     2645..2661
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2645..2661
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     terminator      2662..2708
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2822..2844
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2836..2853
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2837..2853
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2906..3005
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 atgtcgaggc atttatcctt atcaaacaag accatcattg tgggaaacct ccacagatgt
       61 gtgacggagg agattctgtt tgaattgttt cttcaggctg gaccggtgga caaggtgcat
      121 atatccagag atggacagca gtcgccttat gcatgcgttt attataagca tgccgaagct
      181 gttccctatg cagttgaact tctcaatgga atctggctgt atgggcaacc aatcaagtta
      241 cagtgtaata atggaggcca ttatcatagg agtgacgtgt ttgcattcca catggagaat
      301 ggactgataa caaacagtgc tgattctgct tatagggata atatggtttc aaatttaaat
      361 gggtgtccag gtgtggagag tcattctaat ggttggagtg acatggtgta cagcagacct
      421 ctggatgcat gttctccagc tgtgtggtac tgtcctcctc tccagcagtg gatggattct
      481 ggatatcctc cttggacgtc ctgcctgaat gagaatgtgt tgctctgtag gtcagcaggt
      541 gccaaaagac gaccatacgg tgggcactct tcaaacaccc cgacccctct tacacccaca
      601 catgcacagt attttactaa atcagactgt acgctgtaag acccagcttt cttgtacaaa
      661 gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt
      721 caaaataaaa tcattatttg aggctaggtg gaggctcagt gatgataagt ctgcgatggt
      781 ggatgcatgt gtcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcagagggc
      841 acaatcctat tccgcgctat ccgacaatct ccaagacatt aggtggagtt cagttcggcg
      901 tatggcatat gtcgctggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
      961 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     1021 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     1081 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     1141 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     1201 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     1261 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     1321 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     1381 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     1441 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     1501 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     1561 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc tattcaacaa
     1621 agccgccgtc ccgtcaagtc agcgtaaatg ggtagggggc ttcaaatcgt ccgctctgcc
     1681 agtgttacaa ccaattaaca aattctgatt agaaaaactc atcgagcatc aaatgaaact
     1741 gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg
     1801 aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga
     1861 ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat
     1921 caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa agcttatgca
     1981 tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat
     2041 caaccaaacc gttattcatt cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt
     2101 taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat
     2161 caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttcccgg
     2221 ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg
     2281 gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg
     2341 caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc
     2401 gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat
     2461 cagcatccat gttggaattt aatcgcggcc tcgagcaaga cgtttcccgt tgaatatggc
     2521 tcataacacc ccttgtatta ctgtttatgt aagcagacag ttttattgtt catgatgata
     2581 tatttttatc ttgtgcaatg taacatcaga gattttgaga cacaacgtgg ctttcccccg
     2641 ccgctctaga actagtggat ccaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
     2701 cgttttatct gttgtttgtc gcattatacg agacgtccag gttgggatac ctgaaacaaa
     2761 acccatcgta cggccaagga agtctccaat aactgtgatc caccacaagc gccagggttt
     2821 tcccagtcac gacgttgtaa aacgacggcc agtcatgcat aatccgcacg catctggaat
     2881 aaggaagtgc cattccgcct gacctcaaat aatgatttta ttttgactga tagtgacctg
     2941 ttcgttgcaa cagattgatg agcaatgctt ttttataatg ccaactttgt acaaaaaagc
     3001 aggctgcacc
//
