LOCUS       sequence-377812-        2472 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression of RBP(V)-13 x Linker-BioID2-HA on the cell 
            surface via a GPI anchor .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Gao SS, Shi R, Sun J, Tang Y, Zheng Z, Li JF, Li H, Zhang J, Leng Q,
            Xu J, Chen X, Zhao J, Sy MS, Feng L, Li C
  TITLE     GPI-anchored ligand-BioID2-tagging system identifies Galectin-1 
            mediating Zika virus entry.
  JOURNAL   iScience. 2022 Nov 2;25(12):105481. doi: 10.1016/j.isci.2022.105481.
            eCollection 2022 Dec 22.
  PUBMED    36404916
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2022-11-2"; volume: "25(12):105481. doi"; pages: " 
            10.1016/j.isci.2022.105481. eCollection 2022 Dec 22"
COMMENT     Sequence Label: pHAGE-PrP (1-22)-RBP(V)-13 x
            Linker-BioID2-HA-...
FEATURES             Location/Qualifiers
     source          1..2472
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1639..2334
                     /codon_start=1
                     /product="promiscuous R40G mutant of a biotin protein 
                     ligase from Aquifex aeolicus (Kim et al., 2016)"
                     /label=BioID2
                     /translation="FKNLIWLKEVDSTQERLKEWNVSYGTALVADRQTKGRGGLGRKWL
                     SQEGGLYFSFLLNPKEFENLLQLPLVLGLSVSEALEEITEIPFSLKWPNDVYFQEKKVS
                     GVLCELSKDKLIVGIGINVNQREIPEEIKDRATTLYEITGKDWDRKEVLLKVLKRISEN
                     LKKFKEKSFKEFKGKIESKMLYLGEEVKLLGEGKITGKLVGLSEKGGALILTEEGIKEI
                     LSGEFSLRRS"
     CDS             2335..2361
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 atggcgaacc ttggctgctg gatgctggtt ctctttgtgg ccacatggag tgacctgggc
       61 ctctgcaagt tcaccatagt ttttccacac aaccaaaaag gaaactggaa aaatgttcct
      121 tctaattacc attattgccc gtcaagctca gatttaaatt ggcataatga cttaataggc
      181 acagccttac aagtcaaaat gcccaagagt cacaaggcta ttcaagcaga cggttggatg
      241 tgtcatgctt ccaaatgggt cactacttgt gatttccgct ggtatggacc gaagtatata
      301 acacagtcca tccgatcctt cactccatct gtagaacaat gcaaggaaag cattgaacaa
      361 acgaaacaag gaacttggct gaatccaggc ttccctcctc aaagttgtgg atatgcaact
      421 gtgacggatg ccgaagcagt gattgtccag gtgactcctc accatgtgct ggttgatgaa
      481 tacacaggag aatgggttga ttcacagttc atcaacggaa aatgcagcaa ttacatatgc
      541 cccactgtcc ataactctac aacctggcat tctgactata aggtcaaagg gctatgtgat
      601 tctaacctca tttccatgga catcaccttc ttctcagagg acggagagct atcatccctg
      661 ggaaaggagg gcacagggtt cagaagtaac tactttgctt atgaaactgg aggcaaggcc
      721 tgcaaaatgc aatactgcaa gcattgggga gtcagactcc catcaggtgt ctggttcgag
      781 atggctgata aggatctctt tgctgcagcc agattccctg aatgcccaga agggtcaagt
      841 atctctgctc catctcagac ctcagtggat gtaagtctaa ttcaggacgt tgagaggatc
      901 ttggattatt ccctctgcca agaaacctgg agcaaaatca gagcgggtct tccaatctct
      961 ccagtggatc tcagctatct tgctcctaaa aacccaggaa ccggtcctgc tttcaccata
     1021 atcaatggta ccctaaaata ctttgagacc agatacatca gagtcgatat tgctgctcca
     1081 atcctctcaa gaatggtcgg aatgatcagt ggaactacca cagaaaggga actgtgggat
     1141 gactgggcac catatgaaga cgtggaaatt ggacccaatg gagttctgag gaccagttca
     1201 ggatataagt ttcctttata catgattgga catggtatgt tggactccga tcttcatctt
     1261 agctcaaagg ctcaggtgtt cgaacatcct cacattcaag acgctgcttc gcaacttcct
     1321 gatgatgaga gtttattttt tggtgatact gggctatcca aaaatccaat cgagcttgta
     1381 gaaggttggt tcagtagttg gaaaagctct attgcctctg aattcggatc cggtggaggc
     1441 gggtctggag gcgggggtag tggcgggggt ggaagcgggg gtggaggcgg gtcgggtggc
     1501 ggaggtagcg gaggcggtgg aagtggaggc ggtggaagtg gtggaggagg ttctggtggc
     1561 ggaggtagcg gtggcggagg tagcggaggc ggtggaagtg gtggcggagg tagcggaggc
     1621 ggtggatcgg cgcgccgttt caagaacctg atctggctga aggaggtgga cagcacccag
     1681 gagagactga aggagtggaa cgtgagctac ggcaccgccc tggtggccga cagacagacc
     1741 aagggcagag gcggcctggg cagaaagtgg ctgagccagg agggcggcct gtacttcagc
     1801 ttcctgctga accccaagga gttcgagaac ctgctgcagc tgcccctggt gctgggcctg
     1861 agcgtgagcg aggccctgga ggagatcacc gagatcccct tcagcctgaa gtggcccaac
     1921 gacgtgtact tccaggagaa gaaggtgagc ggcgtgctgt gcgagctgag caaggacaag
     1981 ctgatcgtgg gcatcggcat caacgtgaac cagagagaga tccccgagga gatcaaggac
     2041 agagccacca ccctgtacga gatcaccggc aaggactggg acagaaagga ggtgctgctg
     2101 aaggtgctga agagaatcag cgagaacctg aagaagttca aggagaagag cttcaaggag
     2161 ttcaagggca agatcgagag caagatgctg tacctgggcg aggaggtgaa gctgctgggc
     2221 gagggcaaga tcaccggcaa gctggtgggc ctgagcgaga agggcggcgc cctgatcctg
     2281 accgaggagg gcatcaagga gatcctgagc ggcgagttca gcctgagaag aagctaccct
     2341 tacgatgtac cggattacgc atctagagga agtggaacca cttcaggtac tacccgtctt
     2401 ctatctgggc acacgtgttt cacgttgaca ggtttgcttg ggacgctagt aaccatgggc
     2461 ttgctgactt ag
//
