LOCUS       sequence-377814-        2478 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression of BioID2-HA-13 x Linker- RBP(V) on the cell 
            surface via a GPI anchor .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Gao SS, Shi R, Sun J, Tang Y, Zheng Z, Li JF, Li H, Zhang J, Leng Q,
            Xu J, Chen X, Zhao J, Sy MS, Feng L, Li C
  TITLE     GPI-anchored ligand-BioID2-tagging system identifies Galectin-1 
            mediating Zika virus entry.
  JOURNAL   iScience. 2022 Nov 2;25(12):105481. doi: 10.1016/j.isci.2022.105481.
            eCollection 2022 Dec 22.
  PUBMED    36404916
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2022-11-2"; volume: "25(12):105481. doi"; pages: " 
            10.1016/j.isci.2022.105481. eCollection 2022 Dec 22"
COMMENT     Sequence Label: pHAGE-PrP (1-22)-BioID2-HA-13 x Linker-
            RBP(V)...
FEATURES             Location/Qualifiers
     source          1..2478
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             67..762
                     /codon_start=1
                     /product="promiscuous R40G mutant of a biotin protein 
                     ligase from Aquifex aeolicus (Kim et al., 2016)"
                     /label=BioID2
                     /translation="FKNLIWLKEVDSTQERLKEWNVSYGTALVADRQTKGRGGLGRKWL
                     SQEGGLYFSFLLNPKEFENLLQLPLVLGLSVSEALEEITEIPFSLKWPNDVYFQEKKVS
                     GVLCELSKDKLIVGIGINVNQREIPEEIKDRATTLYEITGKDWDRKEVLLKVLKRISEN
                     LKKFKEKSFKEFKGKIESKMLYLGEEVKLLGEGKITGKLVGLSEKGGALILTEEGIKEI
                     LSGEFSLRRS"
     CDS             763..789
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 atggcgaacc ttggctgctg gatgctggtt ctctttgtgg ccacatggag tgacctgggc
       61 ctctgcttca agaacctgat ctggctgaag gaggtggaca gcacccagga gagactgaag
      121 gagtggaacg ttagctacgg caccgccctg gtggccgaca gacagaccaa gggcagaggc
      181 ggcctgggca gaaagtggct gagccaggag ggcggcctgt acttcagctt cctgctgaac
      241 cccaaggagt tcgagaacct gctgcagctg cccctggtgc tgggcctgag cgtgagcgag
      301 gccctggagg agatcaccga gatccccttc agcctgaagt ggcccaacga cgtgtacttc
      361 caggagaaga aggtgagcgg cgtgctgtgc gagctgagca aggacaagct gatcgtgggc
      421 atcggcatca acgtgaacca gagagagatc cccgaggaga tcaaggacag agccaccacc
      481 ctgtacgaga tcaccggcaa ggactgggac agaaaggagg tgctgctgaa ggtgctgaag
      541 agaatcagcg agaacctgaa gaagttcaag gagaagagct tcaaggagtt caagggcaag
      601 atcgagagca agatgctgta cctgggcgag gaggtgaagc tgctgggcga gggcaagatc
      661 accggcaagc tggtgggcct gagcgagaag ggcggcgccc tgatcctgac cgaggagggc
      721 atcaaggaga tcctgagcgg cgagttcagc ctgagaagaa gctaccctta cgatgtaccg
      781 gattacgcag aattcggatc cggtggaggc gggtctggag gcgggggtag tggcgggggt
      841 ggaagcgggg gtggaggcgg gtcgggtggc ggaggtagcg gaggcggtgg aagtggaggc
      901 ggtggaagtg gtggaggagg ttctggtggc ggaggtagcg gtggcggagg tagcggaggc
      961 ggtggaagtg gtggcggagg tagcggaggc ggtggatcgg cgcgccgttc tagaaagttc
     1021 accatagttt ttccacacaa ccaaaaagga aactggaaaa atgttccttc taattaccat
     1081 tattgcccgt caagctcaga tttaaattgg cataatgact taataggcac agccttacaa
     1141 gtcaaaatgc ccaagagtca caaggctatt caagcagacg gttggatgtg tcatgcttcc
     1201 aaatgggtca ctacttgtga tttccgctgg tatggaccga agtatataac acagtccatc
     1261 cgatccttca ctccatctgt agaacaatgc aaggaaagca ttgaacaaac gaaacaagga
     1321 acttggctga atccaggctt ccctcctcaa agttgtggat atgcaactgt gacggatgcc
     1381 gaagcagtga ttgtccaggt gactcctcac catgtgctgg ttgatgaata cacaggagaa
     1441 tgggttgatt cacagttcat caacggaaaa tgcagcaatt acatatgccc cactgtccat
     1501 aactctacaa cctggcattc tgactataag gtcaaagggc tatgtgattc taacctcatt
     1561 tccatggaca tcaccttctt ctcagaggac ggagagctat catccctggg aaaggagggc
     1621 acagggttca gaagtaacta ctttgcttat gaaactggag gcaaggcctg caaaatgcaa
     1681 tactgcaagc attggggagt cagactccca tcaggtgtct ggttcgagat ggctgataag
     1741 gatctctttg ctgcagccag attccctgaa tgcccagaag ggtcaagtat ctctgctcca
     1801 tctcagacct cagtggatgt aagtctaatt caggacgttg agaggatctt ggattattcc
     1861 ctctgccaag aaacctggag caaaatcaga gcgggtcttc caatctctcc agtggatctc
     1921 agctatcttg ctcctaaaaa cccaggaacc ggtcctgctt tcaccataat caatggtacc
     1981 ctaaaatact ttgagaccag atacatcaga gtcgatattg ctgctccaat cctctcaaga
     2041 atggtcggaa tgatcagtgg aactaccaca gaaagggaac tgtgggatga ctgggcacca
     2101 tatgaagacg tggaaattgg acccaatgga gttctgagga ccagttcagg atataagttt
     2161 cctttataca tgattggaca tggtatgttg gactccgatc ttcatcttag ctcaaaggct
     2221 caggtgttcg aacatcctca cattcaagac gctgcttcgc aacttcctga tgatgagagt
     2281 ttattttttg gtgatactgg gctatccaaa aatccaatcg agcttgtaga aggttggttc
     2341 agtagttgga aaagctctat tgcctcttct agaggaagtg gaaccacttc aggtactacc
     2401 cgtcttctat ctgggcacac gtgtttcacg ttgacaggtt tgcttgggac gctagtaacc
     2461 atgggcttgc tgacttag
//
