LOCUS       sequence-377818-        2424 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression of BioID2-HA-13 x Linker- RBP(Z) on the cell 
            surface via a GPI anchor .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Gao SS, Shi R, Sun J, Tang Y, Zheng Z, Li JF, Li H, Zhang J, Leng Q,
            Xu J, Chen X, Zhao J, Sy MS, Feng L, Li C
  TITLE     GPI-anchored ligand-BioID2-tagging system identifies Galectin-1 
            mediating Zika virus entry.
  JOURNAL   iScience. 2022 Nov 2;25(12):105481. doi: 10.1016/j.isci.2022.105481.
            eCollection 2022 Dec 22.
  PUBMED    36404916
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2022-11-2"; volume: "25(12):105481. doi"; pages: " 
            10.1016/j.isci.2022.105481. eCollection 2022 Dec 22"
COMMENT     Sequence Label: pHAGE-PrP (1-22)-BioID2-HA-13 x Linker-
            RBP(Z)...
FEATURES             Location/Qualifiers
     source          1..2424
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             67..762
                     /codon_start=1
                     /product="promiscuous R40G mutant of a biotin protein 
                     ligase from Aquifex aeolicus (Kim et al., 2016)"
                     /label=BioID2
                     /translation="FKNLIWLKEVDSTQERLKEWNVSYGTALVADRQTKGRGGLGRKWL
                     SQEGGLYFSFLLNPKEFENLLQLPLVLGLSVSEALEEITEIPFSLKWPNDVYFQEKKVS
                     GVLCELSKDKLIVGIGINVNQREIPEEIKDRATTLYEITGKDWDRKEVLLKVLKRISEN
                     LKKFKEKSFKEFKGKIESKMLYLGEEVKLLGEGKITGKLVGLSEKGGALILTEEGIKEI
                     LSGEFSLRRS"
     CDS             763..789
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1911..1922
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 atggcgaacc ttggctgctg gatgctggtt ctctttgtgg ccacatggag tgacctgggc
       61 ctctgcttca agaacctgat ctggctgaag gaggtggaca gcacccagga gagactgaag
      121 gagtggaacg ttagctacgg caccgccctg gtggccgaca gacagaccaa gggcagaggc
      181 ggcctgggca gaaagtggct gagccaggag ggcggcctgt acttcagctt cctgctgaac
      241 cccaaggagt tcgagaacct gctgcagctg cccctggtgc tgggcctgag cgtgagcgag
      301 gccctggagg agatcaccga gatccccttc agcctgaagt ggcccaacga cgtgtacttc
      361 caggagaaga aggtgagcgg cgtgctgtgc gagctgagca aggacaagct gatcgtgggc
      421 atcggcatca acgtgaacca gagagagatc cccgaggaga tcaaggacag agccaccacc
      481 ctgtacgaga tcaccggcaa ggactgggac agaaaggagg tgctgctgaa ggtgctgaag
      541 agaatcagcg agaacctgaa gaagttcaag gagaagagct tcaaggagtt caagggcaag
      601 atcgagagca agatgctgta cctgggcgag gaggtgaagc tgctgggcga gggcaagatc
      661 accggcaagc tggtgggcct gagcgagaag ggcggcgccc tgatcctgac cgaggagggc
      721 atcaaggaga tcctgagcgg cgagttcagc ctgagaagaa gctaccctta cgatgtaccg
      781 gattacgcag aattcggatc cggtggaggc gggtctggag gcgggggtag tggcgggggt
      841 ggaagcgggg gtggaggcgg gtcgggtggc ggaggtagcg gaggcggtgg aagtggaggc
      901 ggtggaagtg gtggaggagg ttctggtggc ggaggtagcg gtggcggagg tagcggaggc
      961 ggtggaagtg gtggcggagg tagcggaggc ggtggatcgg cgcgccgttc tagaatcagg
     1021 tgcataggag tcagcaatag ggactttgtg gaaggtatgt caggtgggac ttgggttgat
     1081 gttgtcttgg aacatggagg ttgtgtcacc gtaatggcac aggacaaacc gactgtcgac
     1141 atagagctgg ttacaacaac agtcagcaac atggcggagg taagatccta ctgctatgag
     1201 gcatcaatat cggacatggc ttcggacagc cgctgcccaa cacaaggtga agcctacctt
     1261 gacaagcaat cagacactca atatgtctgc aaaagaacgt tagtggacag aggctgggga
     1321 aatggatgtg gactttttgg caaagggagc ctggtgacat gcgctaagtt tgcatgctcc
     1381 aagaaaatga ccgggaagag catccagcca gagaatctgg agtaccggat aatgctgtca
     1441 gttcatggct cccagcacag tgggatgatc gttaatgaca caggacatga aactgatgag
     1501 aatagagcga aggttgagat aacgcccaat tcaccaagag ccgaagccac cctggggggt
     1561 tttggaagcc taggacttga ttgtgaaccg aggacaggcc ttgacttttc agatttgtat
     1621 tacttgacta tgaataacaa gcactggttg gttcacaagg agtggttcca cgacattcca
     1681 ttaccttggc acgctggggc agacaccgga actccacact ggaacaacaa agaagcactg
     1741 gtagagttca aggacgcaca tgccaaaagg caaactgtcg tggttctagg gagtcaagaa
     1801 ggagcagttc acacggccct tgctggagct ctggaggctg agatggatgg tgcaaaggga
     1861 aggctgtcct ctggccactt gaaatgtcgc ctgaaaatgg ataaacttag attgaagggc
     1921 gtgtcatact ccttgtgtac cgcagcgttc acattcacca agatcccggc tgaaacactg
     1981 cacgggacag tcacagtgga ggtacagtac gcagggacag atggaccttg caaggttcca
     2041 gctcagatgg cggtggacat gcaaactctg accccagttg ggaggttgat aaccgctaac
     2101 cccgtaatca ctgaaagcac tgagaactct aagatgatgc tggaacttga tccaccattt
     2161 ggggactctt acattgtcat aggagtcggg gagaagaaga tcacccacca ctggcacagg
     2221 agtggcagca ccattggaaa agcatttgaa gccactgtga gaggtgccaa gagaatggca
     2281 gtcttgggag acacagcctg ggactttgga tcatctagag gaagtggaac cacttcaggt
     2341 actacccgtc ttctatctgg gcacacgtgt ttcacgttga caggtttgct tgggacgcta
     2401 gtaaccatgg gcttgctgac ttag
//
