LOCUS       sequence-377854-       15610 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15610)
  AUTHORS   Madisen L, Zwingman TA, Sunkin SM, Oh SW, Zariwala HA, Gu H, Ng LL, 
            Palmiter RD, Hawrylycz MJ, Jones AR, Lein ES, Zeng H
  TITLE     A robust and high-throughput Cre reporting and characterization 
            system for the whole mouse brain.
  JOURNAL   Nat Neurosci. 2010 Jan . 13(1):133-40.
  PUBMED    20023653
REFERENCE   2  (bases 1 to 15610)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2010 Jan . 13(1):133-40."
COMMENT     Sequence Label: Ai2
FEATURES             Location/Qualifiers
     source          1..15610
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    3..1085
                     /label=Rosa26 left arm
                     /note="left homology arm from intron 1 of the mouse Rosa26 
                     gene (Soriano, 1999)"
     protein_bind    1096..1129
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     enhancer        1219..1522
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1524..1801
                     /label=chicken beta-actin promoter
     intron          1802..2818
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2742..2764
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2826..2845
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    2894..2927
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    3094..3228
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    3348..3482
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    3602..3736
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    3765..3798
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      3845..3854
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3851..3853,3854..3856,3857..4570)
                     /codon_start=1
                     /product="enhanced yellow variant of GFP"
                     /label=Topaz YFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMRQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3896..3917)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4157..4176)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4504..4525
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    4603..4810
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    4818..4851
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        4873..5372
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     protein_bind    5392..5425
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     regulatory      5426..5435
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5432..6235
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5495..5514)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6105..6124
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6245..6706
                     /label=PGK poly(A) signal
                     /note="mouse phosphoglycerate kinase 1 polyadenylation 
                     signal"
     misc_feature    6772..11112
                     /label=Rosa26 right arm
                     /note="right homology arm from intron 1 of the mouse Rosa26
                     gene (Soriano, 1999)"
     promoter        11162..11661
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             11688..12260
                     /codon_start=1
                     /gene="Corynebacterium diphtheriae tox"
                     /product="diphtheria toxin fragment A"
                     /label=DTA
                     /translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD
                     DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL
                     GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI
                     NFETRGKRGQDAMYEYMAQACAGNRVRR"
     intron          12337..12402
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     polyA_signal    12476..12700
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(12762..12782)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(12762..12780)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(12801..12817)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12801..12817)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12814..12836)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12825..12841
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12849..12855,12856..12873,12874..12879))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12894..12915
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(13032..13049)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13203..13791)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13283..13302)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13962..14753,14754..14822))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14585..14604
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14823..14927)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      14954..15409
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(15041..15060)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     15251..15272
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     15535..15557
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     15549..15566
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     15550..15566
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     15576..15595
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        15576..15594
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ccgcggcagg ccctccgagc gtggtggagc cgttctgtga gacagccggg tacgagtcgt
       61 gacgctggaa ggggcaagcg ggtggtgggc aggaatgcgg tccgccctgc agcaaccgga
      121 gggggaggga gaagggagcg gaaaagtctc caccggacgc ggccatggct cggggggggg
      181 ggggcagcgg aggagcgctt ccggccgacg tctcgtcgct gattggcttc ttttcctccc
      241 gccgtgtgtg aaaacacaaa tggcgtgttt tggttggcgt aaggcgcctg tcagttaacg
      301 gcagccggag tgcgcagccg ccggcagcct cgctctgccc actgggtggg gcgggaggta
      361 ggtggggtga ggcgagctgg acgtgcgggc gcggtcggcc tctggcgggg cgggggrggg
      421 gagggagggt cagcgaaagt agctcgcgcg cgagcggccg cccaccctcc ccttcctctg
      481 ggggagtcgt tttacccgcc gccggccggg cctcgtcgtc tgattggctc tcggggccca
      541 gaaaactggc ccttgccatt ggctcgtgtt cgtgcaagtt gagtccatcc gccggccagc
      601 gggggcggcg aggaggcgct cccaggttcc ggccctcccc tcggccccgc gccgcagagt
      661 ctggccgcgc gcccctgcgc aacgtggcag gaagcgcgcg ctgggggcgg ggacgggcag
      721 tagggctgag cggctgcggg gcgggtgcaa gcacgtttcc gacttgagtt gcctcaagag
      781 gggcgtgctg agccagacct ccatcgcgca ctccggggag tggagggaag gagcgagggc
      841 tcagttgggc tgttttggag gcaggaagca cttgctctcc caaagtcgct ctgagttgtt
      901 atcagtaagg gagctgcagt ggagtaggcg gggagaaggc cgcacccttc tccggagggg
      961 ggaggggagt gttgcaatac ctttctggga gttctctgct gcctcctggc ttctgaggac
     1021 cgccctgggc ctgggagaat cccttccccc tcttccctcg tgatctgcaa ctccagtctt
     1081 tctagcctta attaagaagt tcctattctc tagaaagtat aggaacttcg tcgacattta
     1141 aacaattctg caggaatcta gttattaata gtaatcaatt acggggtcat tagttcatag
     1201 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
     1261 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
     1321 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     1381 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
     1441 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
     1501 attagtcatc gctattacca tggtcgaggt gagccccacg ttctgcttca ctctccccat
     1561 ctcccccccc tccccacccc caattttgta tttatttatt ttttaattat tttgtgcagc
     1621 gatgggggcg gggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
     1681 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
     1741 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
     1801 gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc
     1861 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
     1921 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
     1981 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
     2041 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
     2101 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
     2161 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
     2221 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca
     2281 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
     2341 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
     2401 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg
     2461 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
     2521 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
     2581 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
     2641 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
     2701 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
     2761 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
     2821 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tgatttgata
     2881 ccgcgggccc aatataactt cgtataatgt atgctatacg aagttattcg cgatgaataa
     2941 atgaaagctt gcagatctgc gactctagag gatctgcgac tctagaggat cataatcagc
     3001 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     3061 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     3121 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     3181 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatctg cgactctaga
     3241 ggatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
     3301 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
     3361 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     3421 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
     3481 tctgcgactc tagaggatca taatcagcca taccacattt gtagaggttt tacttgcttt
     3541 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
     3601 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     3661 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     3721 ttatcatgtc tggatcccca tcaagctgat ccggaaccct taatataact tcgtataatg
     3781 tatgctatac gaagttatta ggtccctcga cctgcagccc aagctagatc gaattcggcc
     3841 ggccgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     3901 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
     3961 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
     4021 ctggcccacc ctcgtgacca ccttcggcta cggcgtgcag tgcttcgccc gctaccccga
     4081 ccacatgcgc cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
     4141 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     4201 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     4261 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa
     4321 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt
     4381 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     4441 cgacaaccac tacctgagct accagtccgc cctgagcaaa gaccccaacg agaagcgcga
     4501 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
     4561 gtacaagtaa cctaggtaga gggccggccc tgcaggcctc gactgtgcct tctagttgcc
     4621 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     4681 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     4741 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatggcaggc
     4801 atgctgggga actagtggtg ccagggcgtg cccttgggct ccccgggcgc ggcggccgca
     4861 tcgaattcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag
     4921 cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat
     4981 ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc
     5041 ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa
     5101 tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc
     5161 gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga
     5221 ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg
     5281 cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc
     5341 tgttctcctc ttcctcatct ccgggccttt cgacctgcaa tcgccgctag cgaagttcct
     5401 attctctaga aagtatagga acttcgccac catgggatcg gccattgaac aagatggatt
     5461 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
     5521 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
     5581 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct
     5641 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
     5701 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
     5761 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
     5821 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
     5881 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
     5941 agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgatgatc tcgtcgtgac
     6001 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
     6061 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
     6121 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
     6181 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagggga
     6241 tccgctgtaa gtctgcagaa attgatgatc tattaaacaa taaagatgtc cactaaaatg
     6301 gaagtttttc ctgtcatact ttgttaagaa gggtgagaac agagtaccta cattttgaat
     6361 ggaaggattg gagctacggg ggtgggggtg gggtgggatt agataaatgc ctgctcttta
     6421 ctgaaggctc tttactattg ctttatgata atgtttcata gttggatatc ataatttaaa
     6481 caagcaaaac caaattaagg gccagctcat tcctcccact catgatctat agatctatag
     6541 atctctcgtg ggatcattgt ttttctcttg attcccactt tgtggttcta agtactgtgg
     6601 tttccaaatg tgtcagtttc atagcctgaa gaacgagatc agcagcctct gttccacata
     6661 cacttcattc tcagtattgt tttgccaagt tctaattcca tcagaaagct tgcagaagat
     6721 ctccccaact ggggtaacct ttgagttctc tcagttgggg gggcgcgccg gctagaagat
     6781 gggcgggagt cttctgggca ggcttaaagg ctaacctggt gtgtgggcgt tgtcctgcag
     6841 gggaattgaa caggtgtaaa attggaggga caagacttcc cacagatttt cggttttgtc
     6901 gggaagtttt ttaatagggg caaataagga aaatgggagg ataggtagtc atctggggtt
     6961 ttatgcagca aaactacagg ttattattgc ttgtgatccg cctcggagta ttttccatcg
     7021 aggtagatta aagacatgct cacccgagtt ttatactctc ctgcttgaga tccttactac
     7081 agtatgaaat tacagtgtcg cgagttagac tatgtaagca gaattttaat catttttaaa
     7141 gagcccagta cttcatatcc atttctcccg ctccttctgc agccttatca aaaggtattt
     7201 tagaacactc attttagccc cattttcatt tattatactg gcttatccaa cccctagaca
     7261 gagcattggc attttccctt tcctgatctt agaagtctga tgactcatga aaccagacag
     7321 attagttaca tacaccacaa atcgaggctg tagctggggc ctcaacactg cagttctttt
     7381 ataactcctt agtacacttt ttgttgatcc tttgccttga tccttaattt tcagtgtcta
     7441 tcacctctcc cgtcaggtgg tgttccacat ttgggcctat tctcagtcca gggagtttta
     7501 caacaataga tgtattgaga atccaaccta aagcttaact ttccactccc atgaatgcct
     7561 ctctcctttt tctccattta taaactgagc tattaaccat taatggtttc caggtggatg
     7621 tctcctcccc caatattacc tgatgtatct tacatattgc caggctgata ttttaagaca
     7681 ttaaaaggta tatttcatta ttgagccaca tggtattgat tactgcttac taaaattttg
     7741 tcattgtaca catctgtaaa aggtggttcc ttttggaatg caaagttcag gtgtttgttg
     7801 tctttcctga cctaaggtct tgtgagcttg tattttttct atttaagcag tgctttctct
     7861 tggactggct tgactcatgg cattctacac gttattgctg gtctaaatgt gattttgcca
     7921 agcttcttca ggacctataa ttttgcttga cttgtagcca aacacaagta aaatgattaa
     7981 gcaacaaatg tatttgtgaa gcttggtttt taggttgttg tgttgtgtgt gcttgtgctc
     8041 tataataata ctatccaggg gctggagagg tggctcggag ttcaagagca cagactgctc
     8101 ttccagaagt cctgagttca attcccagca accacatggt ggctcacaac catctgtaat
     8161 gggatctgat gccctcttct ggtgtgtctg aagaccacaa gtgtattcac attaaataaa
     8221 taaatcctcc ttcttcttct tttttttttt tttaaagaga atactgtctc cagtagaatt
     8281 tactgaagta atgaaatact ttgtgtttgt tccaatatgg tagccaataa tcaaattact
     8341 ctttaagcac tggaaatgtt accaaggaac taatttttat ttgaagtgta actgtggaca
     8401 gaggagccat aactgcagac ttgtgggata cagaagacca atgcagactt taatgtcttt
     8461 tctcttacac taagcaataa agaaataaaa attgaacttc tagtatccta tttgtttaaa
     8521 ctgctagctt tacttaactt ttgtgcttca tctatacaaa gctgaaagct aagtctgcag
     8581 ccattactaa acatgaaagc aagtaatgat aattttggat ttcaaaaatg tagggccaga
     8641 gtttagccag ccagtggtgg tgcttgcctt tatgccttta atcccagcac tctggaggca
     8701 gagacaggca gatctctgag tttgagccca gcctggtcta cacatcaagt tctatctagg
     8761 atagccagga atacacacag aaaccctgtt ggggaggggg gctctgagat ttcataaaat
     8821 tataattgaa gcattcccta atgagccact atggatgtgg ctaaatccgt ctacctttct
     8881 gatgagattt gggtattatt ttttctgtct ctgctgttgg ttgggtcttt tgacactgtg
     8941 ggctttcttt aaagcctcct tcctgccatg tggtctcttg tttgctacta acttcccatg
     9001 gcttaaatgg catggctttt tgccttctaa gggcagctgc tgagatttgc agcctgattt
     9061 ccagggtggg gttgggaaat ctttcaaaca ctaaaattgt cctttaattt tttttttaaa
     9121 aaatgggtta tataataaac ctcataaaat agttatgagg agtgaggtgg actaatatta
     9181 aatgagtccc tcccctataa aagagctatt aaggcttttt gtcttatact taactttttt
     9241 tttaaatgtg gtatctttag aaccaagggt cttagagttt tagtatacag aaactgttgc
     9301 atcgcttaat cagattttct agtttcaaat ccagagaatc caaattcttc acagccaaag
     9361 tcaaattaag aatttctgac ttttaatgtt aatttgctta ctgtgaatat aaaaatgata
     9421 gcttttcctg aggcagggtc tcactatgta tctctgcctg atctgcaaca agatatgtag
     9481 actaaagttc tgcctgcttt tgtctcctga atactaaggt taaaatgtag taatactttt
     9541 ggaacttgca ggtcagattc ttttataggg gacacactaa gggagcttgg gtgatagttg
     9601 gtaaaatgtg tttcaagtga tgaaaacttg aattattatc accgcaacct actttttaaa
     9661 aaaaaaagcc aggcctgtta gagcatgctt aagggatccc taggacttgc tgagcacaca
     9721 agagtagtta cttggcaggc tcctggtgag agcatatttc aaaaaacaag gcagacaacc
     9781 aagaaactac agttaaggtt acctgtcttt aaaccatctg catatacaca gggatattaa
     9841 aatattccaa ataatatttc attcaagttt tcccccatca aattgggaca tggatttctc
     9901 cggtgaatag gcagagttgg aaactaaaca aatgttggtt ttgtgatttg tgaaattgtt
     9961 ttcaagtgat agttaaagcc catgagatac agaacaaagc tgctatttcg aggtctcttg
    10021 gtttatactc agaagcactt ctttgggttt ccctgcacta tcctgatcat gtgctaggcc
    10081 taccttaggc tgattgttgt tcaaataaac ttaagtttcc tgtcaggtga tgtcatatga
    10141 tttcatatat caaggcaaaa catgttatat atgttaaaca tttgtactta atgtgaaagt
    10201 taggtctttg tgggtttgat ttttaatttt caaaacctga gctaaataag tcatttttac
    10261 atgtcttaca tttggtggaa ttgtataatt gtggtttgca ggcaagactc tctgacctag
    10321 taaccctacc tatagagcac tttgctgggt cacaagtcta ggagtcaagc atttcacctt
    10381 gaagttgaga cgttttgtta gtgtatacta gtttatatgt tggaggacat gtttatccag
    10441 aagatattca ggactatttt tgactgggct aaggaattga ttctgattag cactgttagt
    10501 gagcattgag tggcctttag gcttgaattg gagtcacttg tatatctcaa ataatgctgg
    10561 ccttttttaa aaagcccttg ttctttatca ccctgttttc tacataattt ttgttcaaag
    10621 aaatacttgt ttggatctcc ttttgacaac aatagcatgt tttcaagcca tatttttttt
    10681 cctttttttt tttttttttg gtttttcgag acagggtttc tctgtatagc cctggctgtc
    10741 ctggaactca ctttgtagac caggctggcc tcgaactcag aaatccgcct gcctctgcct
    10801 cctgagtgcc gggattaaag gcgtgcacca ccacgcctgg ctaagttgga tattttgtta
    10861 tataactata accaatacta actccactgg gtggattttt aattcagtca gtagtcttaa
    10921 gtggtcttta ttggcccttc attaaaatct actgttcact ctaacagagg ctgttggtac
    10981 tagtggcact taagcaactt cctacggata tactagcaga ttaagggtca gggatagaaa
    11041 ctagtctagc gttttgtata cctaccagct ttatactacc ttgttctgat agaaatattt
    11101 caggacatct agcttatcga taccgtcgac ggtatcgata agcttgatat cgaattctac
    11161 cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg
    11221 ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg
    11281 cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc
    11341 aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca
    11401 cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt
    11461 tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg
    11521 ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc
    11581 tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct
    11641 tcctcatctc cgggcctttc gacctgcagg tcctcgccat ggatcctgat gatgttgttg
    11701 attcttctaa atcttttgtg atggaaaact tttcttcgta ccacgggact aaacctggtt
    11761 atgtagattc cattcaaaaa ggtatacaaa agccaaaatc tggtacacaa ggaaattatg
    11821 acgatgattg gaaagggttt tatagtaccg acaataaata cgacgctgcg ggatactctg
    11881 tagataatga aaacccgctc tctggaaaag ctggaggcgt ggtcaaagtg acgtatccag
    11941 gactgacgaa ggttctcgca ctaaaagtgg ataatgccga aactattaag aaagagttag
    12001 gtttaagtct cactgaaccg ttgatggagc aagtcggaac ggaagagttt atcaaaaggt
    12061 tcggtgatgg tgcttcgcgt gtagtgctca gccttccctt cgctgagggg agttctagcg
    12121 ttgaatatat taataactgg gaacaggcga aagcgttaag cgtagaactt gagattaatt
    12181 ttgaaacccg tggaaaacgt ggccaagatg cgatgtatga gtatatggct caagcctgtg
    12241 caggaaatcg tgtcaggcga tctctttgtg aaggaacctt acttctgtgg tgtgacataa
    12301 ttggacaaac tacctacaga gatttaaagc tctaaggtaa atataaaatt tttaagtgta
    12361 taatgtgtta aactactgat tctaattgtt tgtgtatttt agattccaac ctatggaact
    12421 gatgaatggg agcagtggtg gaatgcagat cctagagctc gctgatcagc ctcgactgtg
    12481 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
    12541 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
    12601 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
    12661 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
    12721 agctggggct cgaggggggg cccggtaccc agcttttgtt ccctttagtg agggttaatt
    12781 gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
    12841 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
    12901 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
    12961 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
    13021 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
    13081 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
    13141 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
    13201 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
    13261 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
    13321 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
    13381 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
    13441 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
    13501 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
    13561 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
    13621 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
    13681 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
    13741 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
    13801 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
    13861 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
    13921 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
    13981 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
    14041 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
    14101 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
    14161 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
    14221 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
    14281 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
    14341 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
    14401 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
    14461 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
    14521 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
    14581 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
    14641 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
    14701 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
    14761 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
    14821 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
    14881 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
    14941 aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
    15001 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
    15061 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
    15121 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
    15181 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
    15241 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
    15301 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
    15361 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat
    15421 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
    15481 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt
    15541 cacgacgttg taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat
    15601 tggagctcca
//
