LOCUS       sequence-377861-        4460 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral deliviery of OFF VIPER CAR (anti-CD19)-candidate 
            design(c+iii).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4460)
  AUTHORS   Li HS, Wong NM, Tague E, Ngo JT, Khalil AS, Wong WW
  TITLE     High-performance multiplex drug-gated CAR circuits.
  JOURNAL   Cancer Cell. 2022 Aug 26. pii: S1535-6108(22)00372-5. doi: 
            10.1016/j.ccell.2022.08.008.
  PUBMED    36084652
REFERENCE   2  (bases 1 to 4460)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Cell. 2022 Aug 26. pii: S1535-6108(22)00372-5. doi: 
            10.1016/j.ccell.2022.08.008."
COMMENT     Sequence Label: pHR-OFF VIPER CAR (anti-CD19)-candidate (c+iii)
FEATURES             Location/Qualifiers
     source          1..4460
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        6..1184
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          237..1175
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1132..1152
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1221..1230
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2016..2057
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2562..2618
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3750..4460
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3895..3913)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4163..4182
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 cgtgaggctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt
       61 tggggggagg ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg gtaaactggg
      121 aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa
      181 gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggtaa
      241 gtgccgtgtg tggttcccgc gggcctggcc tctttacggg ttatggccct tgcgtgcctt
      301 gaattacttc cacctggctg cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg
      361 ggtgggagag ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct tgagttgagg
      421 cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg
      481 ctgctttcga taagtctcta gccatttaaa atttttgatg acctgctgcg acgctttttt
      541 tctggcaaga tagtcttgta aatgcgggcc aagatctgca cactggtatt tcggtttttg
      601 gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc
      661 tgcgagcgcg gccaccgaga atcggacggg ggtagtctca agctggccgg cctgctctgg
      721 tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg
      781 caccagttgc gtgagcggaa agatggccgc ttcccggccc tgctgcaggg agctcaaaat
      841 ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct
      901 ttccgtcctc agccgtcgct tcatgtgact ccacggagta ccgggcgccg tccaggcacc
      961 tcgattagtt ctcgagcttt tggagtacgt cgtctttagg ttggggggag gggttttatg
     1021 cgatggagtt tccccacact gagtgggtgg agactgaagt taggccagct tggcacttga
     1081 tgtaattctc cttggaattt gccctttttg agtttggatc ttggttcatt ctcaagcctc
     1141 agacagtggt tcaaagtttt tttcttccat ttcaggtgtc gtgaggggat ctggagctct
     1201 cgagaattct cacgcgtgcc gccaccatgg ccttaccagt gaccgccttg ctcctgccgc
     1261 tggccttgct gctccacgcc gccaggccgg acatccagat gacacagact acatcctccc
     1321 tgtctgcctc tctgggagac agagtcacca tcagttgcag ggcaagtcag gacattagta
     1381 aatatttaaa ttggtatcag cagaaaccag atggaactgt taaactcctg atctaccata
     1441 catcaagatt acactcagga gtcccatcaa ggttcagtgg cagtgggtct ggaacagatt
     1501 attctctcac cattagcaac ctggagcaag aagatattgc cacttacttt tgccaacagg
     1561 gtaatacgct tccgtacacg ttcggaggcg gcaccaagct ggagatcaca ggtggcggtg
     1621 gctcgggcgg tggtgggtcg ggtggcggcg gatctgaggt gaaactgcag gagtcaggac
     1681 ctggcctggt ggcgccctca cagagcctgt ccgtcacatg cactgtctca ggggtctcat
     1741 tacccgacta tggtgtaagc tggattcgcc agcctccacg aaagggtctg gagtggctgg
     1801 gagtaatatg gggtagtgaa accacatact ataattcagc tctcaaatcc agactgacca
     1861 tcatcaagga caactccaag agccaagttt tcttaaaaat gaacagtctg caaactgatg
     1921 acacagccat ttactactgt gccaaacatt attactacgg tggtagctat gctatggact
     1981 actggggcca aggaacctca gtcaccgtct cctcagggaa gcctatcccg aaccctctgt
     2041 tgggtctcga tagtaccaat ggggccgcaa ccacgacgcc agcgccgcga ccaccaacac
     2101 cggcgcccac catcgcgttg cagcccctgt ccctgcgccc agaggcgtgc cggccagcgg
     2161 cggggggcgc agtgcacacg agggggctgg acttcgcctg tgatttttgg gtgctggtgg
     2221 tggttggtgg agtcctggct tgctatagct tgctagtaac agtggccttt attattttct
     2281 gggtgaggag taagaggagc aggctcctgc acagtgacta catgaacatg actccccgcc
     2341 gccccgggcc cacccgcaag cattaccagc cctatgcccc accacgcgac ttcgcagcct
     2401 atcgctccgg gggtaccgaa ttcagcgggt caggcggatc tggaagtggt ggtggagaac
     2461 ttgatgaatt ggtatactta ctagatgggc caggttatga ccctatacat tgcgatgtag
     2521 tgacaagggg cggcagccac cttttcaatt ttggaagcgg agctactaac ttcagcctgc
     2581 tgaagcaggc tggagacgtg gaggagaacc ctggacctat gattcatctt ggtcacatcc
     2641 tcttcttgct gctgttgcca gttgctgccg cacaaacaac cccaggtgag aggtcaagtc
     2701 ttcccgcctt ctatccgggc acaagcggga gttgtagtgg gtgtggatct ttgtcccttc
     2761 ctatatacat ctgggctcct ctggctggca cttgcggagt gcttctgctg agtctggtta
     2821 ttaccggttc tgttgttatt gttggtagaa ttattttatc tggtagtggt agtatcacgg
     2881 cctactccca acagacgcgg ggcctacttg gttgcatcat cactagcctc acaggccggg
     2941 acaagaacca ggtcgaaggg gaggttcaag tggtttctac cgcaacacaa tctttcctgg
     3001 cgacctgcgt caacggcgtg tgctggactg tctaccatgg cgctggctcg aagaccctag
     3061 ccggtccaaa aggtccaatc acccaaatgt acaccaatgt agaccaggac ctcgtcggct
     3121 ggcaggcgcc tccaggggcg cgctccttga caccatgcac ctgtggcagc tcggaccttt
     3181 acttggtcac gagacatgct gatgtcattc cggtgcgccg gcgaggcgac agcaggggaa
     3241 gtctactctc ccccaggccc gtctcctacc tgaaaggctc cgcaggtggt ccattgcttt
     3301 gcccttcggg gcacgctgtg ggcatcttcc gggctgctgt gtgcacccgg ggggtcgcga
     3361 aggcggtgga cttcgtgccc gttgagtcta tggaaactac catgcggtct agagtgaagt
     3421 tcagcaggag cgcagacgcc cccgcgtaca agcagggcca gaaccagctc tataacgagc
     3481 tcaatctagg acgaagagag gagtacgatg ttttggacaa gagacgtggc cgggaccctg
     3541 agatgggggg aaagccgaga aggaagaacc ctcaggaagg cctgtacaat gaactgcaga
     3601 aagataagat ggcggaggcc tacagtgaga ttgggatgaa aggcgagcgc cggaggggca
     3661 aggggcacga tggcctttac cagggtctca gtacagccac caaggacacc tacgacgccc
     3721 ttcacatgca ggccctgccc cctcgcggca tggtgagcaa gggcgaggag gataacatgg
     3781 ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg
     3841 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc
     3901 tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca
     3961 tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt
     4021 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga
     4081 ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg
     4141 gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct
     4201 cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga
     4261 agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc
     4321 ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg
     4381 aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca
     4441 tggacgagct gtacaagtaa
//
