LOCUS       sequence-377868-        6769 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector for an inducible 3xFLAG-CSE1L.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6769)
  AUTHORS   Wang L, Paudel BB, McKnight RA, Janes KA
  TITLE     Nucleocytoplasmic transport of active HER2 causes fractional escape 
            from the DCIS-like state.
  JOURNAL   Nat Commun. 2023 Apr 13;14(1):2110. doi: 10.1038/s41467-023-37914-x.
  PUBMED    37055441
REFERENCE   2  (bases 1 to 6769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-04-13"; volume: "14(1):2110. doi"; pages: " 
            10.1038/s41467-023-37914-x"
COMMENT     Sequence Label: pEN_TT 3xFLAG-CSE1L
FEATURES             Location/Qualifiers
     source          1..6769
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     25..48
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    67..166
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        198..512
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    206..224
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    242..260
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    277..295
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    313..331
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    349..367
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    384..402
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    420..438
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     485..509
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             522..587
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(3539..3638)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3750..3769)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(3828..3847)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     CDS             complement(4421..4954)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(5143..5148,5149..5165,5166..5171))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      5828..6416
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6317..6336
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      6581..6667
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      join(6759..6769,1..17)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ctgacggatg gcctttttgc gtttctacaa actcttcctg ttagttagtt acttaagctc
       61 gggccccaaa taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat
      121 aagcaatgct tttttataat gccaactttg tacaaaaaag caggctttaa aggaaccaat
      181 tcagtcgact ggatcccgag tttactccct atcagtgata gagaacgtat gtcgagttta
      241 ctccctatca gtgatagaga acgatgtcga gtttactccc tatcagtgat agagaacgta
      301 tgtcgagttt actccctatc agtgatagag aacgtatgtc gagtttactc cctatcagtg
      361 atagagaacg tatgtcgagt ttatccctat cagtgataga gaacgtatgt cgagtttact
      421 ccctatcagt gatagagaac gtatgtcgag gtaggcgtgt acggtgggag gcctatataa
      481 gcagagctcg tttagtgaac cgtcagatcg ccactagaat ggactacaaa gaccatgacg
      541 gtgattataa agatcatgac atcgattaca aggatgacga tgacaagatg gaactcagcg
      601 atgcaaatct gcaaacacta acagaatatt taaagaaaac acttgatcct gatcctgcca
      661 tccgacgtcc agctgagaaa tttcttgaat ctgttgaagg aaatcagaat tatccactgt
      721 tgcttttgac attactggag aagtcccagg ataatgttat caaagtatgt gcttcagtaa
      781 cattcaaaaa ctatattaaa aggaactgga gaattgttga agatgaacca aacaaaattt
      841 gtgaagccga tcgagtggcc attaaagcca acatagtgca cttgatgctt agcagcccag
      901 agcaaattca gaagcagtta agtgatgcaa ttagcattat tggcagagaa gattttccac
      961 agaaatggcc tgacttgctg acagaaatgg tgaatcgctt tcagagtgga gatttccatg
     1021 ttattaatgg agtcctccgt acagcacatt cattatttaa aagataccgt catgaattta
     1081 agtcaaacga gttatggact gaaattaagc ttgttctgga tgcctttgct ttgcctttga
     1141 ctaatctttt taaggccact attgaactct gcagtaccca tgcaaatgat gcctctgccc
     1201 tgaggattct gttttcttcc ctgatcctga tctcaaaatt gttctatagt ttaaactttc
     1261 aggatctccc tgaatttttt gaagataata tggaaacttg gatgaataat tttcatactc
     1321 tcttaacatt ggataataag cttttacaaa ctgatgatga agaggaagcc ggcttattgg
     1381 agctcttaaa atcccagatt tgtgataatg ccgcactcta tgcacaaaag tacgatgaag
     1441 aattccagcg atacctgcct cgttttgtta cagccatctg gaatttacta gttacaacgg
     1501 gtcaagaggt taaatatgat ttgttggtaa gtaatgcaat tcaatttctg gcttcagttt
     1561 gtgagagacc tcattataag aatctatttg aggaccagaa cacgctgaca agtatctgtg
     1621 aaaaggttat tgtgcctaac atggaattta gagctgctga tgaagaagca tttgaagata
     1681 attctgagga gtacataagg agagatttgg aaggatctga tattgatact agacgcaggg
     1741 ctgcttgtga tctggtacga ggattatgca agttttttga gggacctgtg acaggaatct
     1801 tctctggtta tgttaattcc atgctgcagg aatacgcaaa aaatccatct gtcaactgga
     1861 aacacaaaga tgcagccatc tacctagtga catctttggc atcaaaagcc caaacacaga
     1921 agcatggaat tacacaagca aatgaacttg taaacctaac tgagttcttt gtgaatcaca
     1981 tcctccctga tttaaaatca gctaatgtga atgaatttcc tgtccttaaa gctgacggta
     2041 tcaaatatat tatgattttt agaaatcaag tgccaaaaga acatctttta gtctcgattc
     2101 ctctcttgat taatcatctt caagctgaaa gtattgttgt tcatacttac gcagctcatg
     2161 ctcttgaacg gctctttact atgcgagggc ctaacaatgc cactctcttt acagctgcag
     2221 aaatcgcacc gtttgttgag attctgctaa caaacctttt caaagctctc acacttcctg
     2281 gctcttcaga aaatgaatat attatgaaag ctatcatgag aagtttttct ctcctacaag
     2341 aagccataat cccctacatc cctactctca tcactcagct tacacagaag ctattagctg
     2401 ttagtaagaa cccaagcaaa cctcacttta atcactacat gtttgaagca atatgtttat
     2461 ccataagaat aacttgcaaa gctaaccctg ctgctgttgt aaattttgag gaggctttgt
     2521 ttttggtgtt tactgaaatc ttacaaaatg atgtgcaaga atttattcca tacgtctttc
     2581 aagtgatgtc tttgcttctg gaaacacaca aaaatgacat cccgtcttcc tatatggcct
     2641 tatttcctca tctccttcag ccagtgcttt gggaaagaac aggaaatatt cctgctctag
     2701 tgaggcttct tcaagcattc ttagaacgcg gttcaaacac aatagcaagt gctgcagctg
     2761 acaaaattcc tgggttacta ggtgtctttc agaagctgat tgcatccaaa gcaaatgacc
     2821 accaaggttt ttatcttcta aacagtataa tagagcacat gcctcctgaa tcagttgacc
     2881 aatataggaa acaaatcttc attctgctat tccagagact tcagaattcc aaaacaacca
     2941 agtttatcaa gagtttttta gtctttatta atttgtattg cataaaatat ggggcactag
     3001 cactacaaga aatatttgat ggtatacaac caaaaatgtt tggaatggtt ttggaaaaaa
     3061 ttattattcc tgaaattcag aaggtatctg gaaatgtaga gaaaaagatc tgtgcggttg
     3121 gcataaccaa attactaaca gaatgtcccc caatgatgga cactgagtat accaaactgt
     3181 ggactccatt attacagtct ttgattggtc tttttgagtt acccgaagat gataccattc
     3241 ctgatgagga acattttatt gacatagaag atacaccagg atatcagact gccttctcac
     3301 agttggcatt tgctgggaaa aaagagcatg atcctgtagg tcaaatggtg aataacccca
     3361 aaattcacct ggcacagtca cttcacaagt tgtctaccgc ctgtccagga agggttccat
     3421 caatggtgag caccagcctg aatgcagaag cgctccagta tctccaaggg taccttcagg
     3481 cagccagtgt gacactgctt taacaattgg aagactaatg cgttttcgag atatctagac
     3541 ccagctttct tgtacaaagt tggcattata agaaagcatt gcttatcaat ttgttgcaac
     3601 gaacaggtca ctatcagtca aaataaaatc attatttgcc atccagctgc agctctggcc
     3661 cgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat
     3721 aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga
     3781 aacgtcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc
     3841 tcgcgataat gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc
     3901 gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat
     3961 ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc attttatccg
     4021 tactcctgat gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccaggt
     4081 attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgtccctgcg
     4141 ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct
     4201 cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga
     4261 gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc
     4321 accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg
     4381 gaaattaggc cgggaagccg atctcggctt gaacgaattg ttaggtggcg gtacttgggt
     4441 cgatatcaaa gtgcatcact tcttcccgta tgcccaactt tgtatagaga gccactgcgg
     4501 gatcgtcacc gtaatctgct tgcacgtaga tcacataagc accaagcgcg ttggcctcat
     4561 gcttgaggag attgatgagc gcggtggcaa tgccctgcct ccggtgctcg ccggagactg
     4621 cgagatcata gatatagatc tcactacgcg gctgctcaaa cttgggcaga acgtaagccg
     4681 cgagagcgcc aacaaccgct tcttggtcga aggcagcaag cgcgatgaat gtcttactac
     4741 ggagcaagtt cccgaggtaa tcggagtccg gctgatgttg ggagtaggtg gctacgtctc
     4801 cgaactcacg accgaaaaga tcaagagcag cccgcatgga tttgacttgg tcagggccga
     4861 gcctacatgt gcgaatgatg cccatacttg agccacctaa ctttgtttta gggcgactgc
     4921 cctgctgcgt aacatcgttg ctgctgcgta acatcgttgc tgctccataa catcaaacat
     4981 cgacccacgg cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg tacaaaaaaa
     5041 cagtcataac aagccatgaa aaccgccact gcgccgttac caccgctgcg ttcggtcaag
     5101 gttctggacc agttgcgtga gcgcatacgc tacttgcatt acagtttacg aaccgaacag
     5161 gcttatgtca actgggttcg tgccttcatc cgtttccacg gtgtgcgtca cccggcaacc
     5221 ttgggcagca gcgaagtcga ggcatttctg tcctggctgg cgaacgagcg caaggtttcg
     5281 gtctccacgc atcgtcaggc attggcggcc ttgctgttct tctacggcaa ggtgctgtgc
     5341 acggatctgc cctggcttca ggagatcgga agacctcggc cgtcgcggcg cttgccggtg
     5401 gtgctgaccc cggatgaagt ggttcgcatc ctcggttttc tggaaggcga gcatcgtttg
     5461 ttcgcccagg actctagcta tagttctagt ggttggctac taataggttg tattgatgtt
     5521 ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt
     5581 gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat
     5641 atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcaga attggttaat
     5701 tggttgtaac actggcagag cattacgctg acttgacggg acggcgcaag ctcatgacca
     5761 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     5821 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     5881 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     5941 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     6001 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     6061 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     6121 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     6181 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     6241 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     6301 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     6361 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     6421 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     6481 ttcctgcgtt atcccctgat tctgtggata accgtattac cgctagcatg gatctcgggg
     6541 acgtctaact actaagcgag agtagggaac tgccaggcat caaataaaac gaaaggctca
     6601 gtcggaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag
     6661 gacaaatccg ccgggagcgg atttgaacgt tgtgaagcaa cggcccggag ggtggcgggc
     6721 aggacgcccg ccataaactg ccaggcatca aactaagcag aaggccatc
//
