LOCUS       sequence-377871-        4162 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast gene induction with meiotic promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4162)
  AUTHORS   Dawson, D.
  TITLE     Dawson Lab Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4162)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pFA6a-kanMX6-pSAE3
FEATURES             Location/Qualifiers
     source          1..4162
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gene            336..1692
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        336..679
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             680..1489
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(747..766)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      1495..1692
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(1796..1815)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1797..1815)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1902..1919)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2073..2661)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2153..2172)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2832..3623,3624..3692))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3455..3474
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3693..3797)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3865..3883
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3921..3943)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4043..4062
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        4143..4161
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     4143..4160
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 cgcggccgcc agctgaagct tcgtacgcca ttttgagatc cgggttttac ttgcaagttg
       61 tcttctgttt atggcaagga taagaccatt gcttttaatt taaaattatt cttatactct
      121 gtctttgcat ttatgaagca gaaatagaat agccgccatt aggcagaaca attccgtctg
      181 atgtgataat gcaaagtgtg cctgtcatct aacttaaaag gaatcaggcc aaccgctaca
      241 ggatatgtac tagatacaat tactaaataa tacaaacaga atatataaaa tgctatgttt
      301 agcttgcctc gtccccgccg ggtcacccgg ccagcgacat ggaggcccag aataccctcc
      361 ttgacagtct tgacgtgcgc agctcagggg catgatgtga ctgtcgcccg tacatttagc
      421 ccatacatcc ccatgtataa tcatttgcat ccatacattt tgatggccgc acggcgcgaa
      481 gcaaaaatta cggctcctcg ctgcagacct gcgagcaggg aaacgctccc ctcacagacg
      541 cgttgaattg tccccacgcc gcgcccctgt agagaaatat aaaaggttag gatttgccac
      601 tgaggttctt ctttcatata cttcctttta aaatcttgct aggatacagt tctcacatca
      661 catccgaaca taaacaacca tgggtaagga aaagactcac gtttcgaggc cgcgattaaa
      721 ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc
      781 aggtgcgaca atctatcgat tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca
      841 tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac
      901 ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt
      961 actcaccact gcgatccccg gcaaaacagc attccaggta ttagaagaat atcctgattc
     1021 aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt
     1081 ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat
     1141 gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga
     1201 acaagtctgg aaagaaatgc ataagctttt gccattctca ccggattcag tcgtcactca
     1261 tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga
     1321 tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct
     1381 cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc
     1441 tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaat cagtactgac
     1501 aataaaaaga ttcttgtttt caagaacttg tcatttgtat agttttttta tattgtagtt
     1561 gttctatttt aatcaaatgt tagcgtgatt tatatttttt ttcgcctcga catcatctgc
     1621 ccagatgcga agttaagtgc gcagaaagta atatcatgcg tcaatcgtat gtgaatgctg
     1681 gtcgctatac tgctgtcgat tcgatactaa cgccgccatc cagtttaaac gagctcgaat
     1741 tcatcgatga tatcagatcc actagtggcc tatgcggccg cggatctgcc ggtctcccta
     1801 tagtgagtcg tattaatttc gataagccag gttaacctgc attaatgaat cggccaacgc
     1861 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     1921 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     1981 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     2041 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     2101 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     2161 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     2221 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     2281 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     2341 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     2401 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     2461 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     2521 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     2581 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     2641 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     2701 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     2761 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     2821 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     2881 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     2941 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     3001 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     3061 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     3121 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     3181 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     3241 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     3301 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     3361 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     3421 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     3481 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     3541 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     3601 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     3661 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     3721 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     3781 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     3841 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt
     3901 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
     3961 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
     4021 tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg
     4081 gacatattgt cgttagaacg cggctacaat taatacataa ccttatgtat catacacata
     4141 cgatttaggt gacactatag aa
//
