LOCUS       sequence-377872-        4334 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast gene induction with meiotic promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4334)
  AUTHORS   Dawson, D.
  TITLE     Dawson Lab Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4334)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pFA6a-kanMX6-pSPR3
FEATURES             Location/Qualifiers
     source          1..4334
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gene            504..1860
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        504..847
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             848..1657
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(915..934)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      1663..1860
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(1964..1983)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1965..1983)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2070..2087)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2241..2829)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2321..2340)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3000..3791,3792..3860))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3623..3642
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3861..3965)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4033..4051
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4089..4111)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4211..4230
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        4311..4329
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     4311..4328
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 gccgccagct gaagcttcgt acgccatttt gagatccggg ttttaataaa aattcaatga
       61 cgcaaaagga attttaggtt tttattattt atttatttat ttctgttaat tgatccttct
      121 ctttccacta ccaacaacaa aaaagggggg aaaaagatgt ataatctaaa agacactaat
      181 ctgctcttga tatccttatt atgtaatgga ataactcata taaatgtaaa atagaacttc
      241 aaattaatat tataatgata gtcgaggtca gacacactta taatacatta agtaaagaaa
      301 aaaaaatgtc tgtcatcgag gtctcttttg tgtcgctaac aaaacatcac taaatacgaa
      361 gacactttgc atgggaagga tgcagcaaat ggcaaactaa cgggccattg attggtttac
      421 ctcttctatt tgtattacga ccagaaagaa cgaatggttt tcatgtttag cttgcctcgt
      481 ccccgccggg tcacccggcc agcgacatgg aggcccagaa taccctcctt gacagtcttg
      541 acgtgcgcag ctcaggggca tgatgtgact gtcgcccgta catttagccc atacatcccc
      601 atgtataatc atttgcatcc atacattttg atggccgcac ggcgcgaagc aaaaattacg
      661 gctcctcgct gcagacctgc gagcagggaa acgctcccct cacagacgcg ttgaattgtc
      721 cccacgccgc gcccctgtag agaaatataa aaggttagga tttgccactg aggttcttct
      781 ttcatatact tccttttaaa atcttgctag gatacagttc tcacatcaca tccgaacata
      841 aacaaccatg ggtaaggaaa agactcacgt ttcgaggccg cgattaaatt ccaacatgga
      901 tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat
      961 ctatcgattg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag
     1021 cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc
     1081 tcttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc
     1141 gatccccggc aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat
     1201 tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc
     1261 ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt
     1321 ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa
     1381 agaaatgcat aagcttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc
     1441 acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt
     1501 cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc
     1561 tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa
     1621 attgcagttt catttgatgc tcgatgagtt tttctaatca gtactgacaa taaaaagatt
     1681 cttgttttca agaacttgtc atttgtatag tttttttata ttgtagttgt tctattttaa
     1741 tcaaatgtta gcgtgattta tatttttttt cgcctcgaca tcatctgccc agatgcgaag
     1801 ttaagtgcgc agaaagtaat atcatgcgtc aatcgtatgt gaatgctggt cgctatactg
     1861 ctgtcgattc gatactaacg ccgccatcca gtttaaacga gctcgaattc atcgatgata
     1921 tcagatccac tagtggccta tgcggccgcg gatctgccgg tctccctata gtgagtcgta
     1981 ttaatttcga taagccaggt taacctgcat taatgaatcg gccaacgcgc ggggagaggc
     2041 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     2101 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     2161 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     2221 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     2281 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     2341 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     2401 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     2461 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     2521 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     2581 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     2641 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     2701 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     2761 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     2821 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     2881 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     2941 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     3001 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     3061 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     3121 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     3181 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     3241 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     3301 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     3361 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     3421 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     3481 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     3541 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     3601 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     3661 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     3721 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     3781 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     3841 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     3901 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     3961 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
     4021 ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac
     4081 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
     4141 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg
     4201 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgga catattgtcg
     4261 ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg atttaggtga
     4321 cactatagaa cgcg
//
