LOCUS       sequence-377874-        4106 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast gene induction with meiotic promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4106)
  AUTHORS   Dawson, D.
  TITLE     Dawson Lab Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4106)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pFA6a-HISMX6-pSPO11
FEATURES             Location/Qualifiers
     source          1..4106
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(5..23)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6..23)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(104..123)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     223..245
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(283..301)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        369..473
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(474..542,543..1334)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(692..711)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1505..2093
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1994..2013
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2247..2264
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     2351..2370
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2351..2369
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      complement(2474..2671)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(2675..3373)
                     /codon_start=1
                     /product="S. kluyveri imidazoleglycerol phosphate 
                     dehydratase"
                     /label=k-HIS3
                     /translation="MAEPAQKKQKQTVQERKAFISRITNETKIQIAISLNGGYIQIKDS
                     ILPAKKDDDVASQATQSQVIDIHTGVGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHT
                     TEDCGIALGQAFKEAMGAVRGVKRFGTGFAPLDEALSRAVVDLSSRPFAVIDLGLKREM
                     IGDLSTEMIPHFLESFAEAARITLHVDCLRGFNDHHRSESAFKALAVAIREAISSNGTN
                     DVPSTKGVLM"
     promoter        complement(3374..3717)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 gcgttctata gtgtcaccta aatcgtatgt gtatgataca taaggttatg tattaattgt
       61 agccgcgttc taacgacaat atgtccatat ggtgcactct cagtacaatc tgctctgatg
      121 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
      181 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
      241 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
      301 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
      361 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
      421 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
      481 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
      541 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
      601 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
      661 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
      721 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
      781 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
      841 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
      901 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
      961 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     1021 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     1081 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     1141 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     1201 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     1261 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     1321 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     1381 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     1441 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     1501 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     1561 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     1621 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     1681 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     1741 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     1801 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     1861 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     1921 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     1981 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     2041 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     2101 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     2161 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     2221 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     2281 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagg ttaacctggc
     2341 ttatcgaaat taatacgact cactataggg agaccggcag atccgcggcc gcataggcca
     2401 ctagtggatc tgatatcatc gatgaattcg agctcgttta aactggatgg cggcgttagt
     2461 atcgaatcga cagcagtata gcgaccagca ttcacatacg attgacgcat gatattactt
     2521 tctgcgcact taacttcgca tctgggcaga tgatgtcgag gcgaaaaaaa atataaatca
     2581 cgctaacatt tgattaaaat agaacaacta caatataaaa aaactataca aatgacaagt
     2641 tcttgaaaac aagaatcttt ttattgtcag tacttcacat caaaacacct ttggttgagg
     2701 gaacgtcatt ggtgccattg ctagaaatag cttctcttat ggcaacagcc aaagccttga
     2761 acgcactctc acttctgtgg tgatcgttga aacctctcag acaatcaaca tgcaaagtaa
     2821 ttctggccgc ctccgcgaaa ctttccaaaa agtgtggaat catttcagtg gataaatcac
     2881 caatcatctc tctcttcaat ccaaggtcga ttacagcaaa tggtctacta gataaatcga
     2941 ctacggcacg tgatagcgcc tcatccaatg gtgcgaaccc agtaccgaat ctttttacac
     3001 cacggacagc acccattgct tctttgaacg cttgccctaa tgcgataccg caatcttcgg
     3061 tagtatggtg atcgtcaatg tgcaggtcac caatacattc aacaataaga gaccaaccag
     3121 agtgttttgc caacgcatgg atcatatgat ccaaaaagcc aacacctgtg tgaatatcga
     3181 tgacctgtga ctgagtagct tgggaagcta catcgtcatc cttctttgca ggaagaatcg
     3241 aatcttttat ttgaatataa ccaccattca gcgaaatagc gatttgaatt ttagtttcat
     3301 tagtgatacg ggagataaac gccttgcgct cctgaacagt ttgtttttgc tttttttggg
     3361 ctggttctgc catggttgtt tatgttcgga tgtgatgtga gaactgtatc ctagcaagat
     3421 tttaaaagga agtatatgaa agaagaacct cagtggcaaa tcctaacctt ttatatttct
     3481 ctacaggggc gcggcgtggg gacaattcaa cgcgtctgtg aggggagcgt ttccctgctc
     3541 gcaggtctgc agcgaggagc cgtaattttt gcttcgcgcc gtgcggccat caaaatgtat
     3601 ggatgcaaat gattatacat ggggatgtat gggctaaatg tacgggcgac agtcacatca
     3661 tgcccctgag ctgcgcacgt caagactgtc aaggagggta ttctgggcct ccatgtcgct
     3721 ggccgggtga cccggcgggg acgaggcaag ctaaacactt cattttttca gcatagttat
     3781 gtgattcttt atatttattt attttctctc tgtgtagtga caattttttt tttaatgttt
     3841 catgttaaat atatttattt taagaagccg tcagtttcct tttgtttcat tgaaatcaat
     3901 aactaaaatt aaaattataa aaggaataac gtaaaagaaa gtctactacc caaggaaaat
     3961 ctactttatt tagtgattat atccaactaa atatcattca aataacctca catatttgtc
     4021 ttcaccctta agatttgacg atttactaag ttcaccttct caaaacccgg atctcaaaat
     4081 ggcgtacgaa gcttcagctg gcggcc
//
