LOCUS       sequence-377875-        4049 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast gene induction with meiotic promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4049)
  AUTHORS   Dawson, D.
  TITLE     Dawson Lab Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4049)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pFA6a-HISMX6-pSAE3
FEATURES             Location/Qualifiers
     source          1..4049
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(10..28)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(11..28)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(109..128)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     228..250
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(288..306)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        374..478
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(479..547,548..1339)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(697..716)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1510..2098
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1999..2018
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2252..2269
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     2356..2375
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2356..2374
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      complement(2479..2676)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(2680..3378)
                     /codon_start=1
                     /product="S. kluyveri imidazoleglycerol phosphate 
                     dehydratase"
                     /label=k-HIS3
                     /translation="MAEPAQKKQKQTVQERKAFISRITNETKIQIAISLNGGYIQIKDS
                     ILPAKKDDDVASQATQSQVIDIHTGVGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHT
                     TEDCGIALGQAFKEAMGAVRGVKRFGTGFAPLDEALSRAVVDLSSRPFAVIDLGLKREM
                     IGDLSTEMIPHFLESFAEAARITLHVDCLRGFNDHHRSESAFKALAVAIREAISSNGTN
                     DVPSTKGVLM"
     promoter        complement(3379..3722)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 cggccgcgtt ctatagtgtc acctaaatcg tatgtgtatg atacataagg ttatgtatta
       61 attgtagccg cgttctaacg acaatatgtc catatggtgc actctcagta caatctgctc
      121 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
      181 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
      241 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg
      301 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
      361 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
      421 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
      481 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
      541 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
      601 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
      661 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
      721 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
      781 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
      841 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
      901 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
      961 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     1021 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     1081 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     1141 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     1201 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     1261 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     1321 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     1381 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     1441 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     1501 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     1561 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     1621 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     1681 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     1741 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     1801 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     1861 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     1921 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     1981 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     2041 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     2101 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     2161 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     2221 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     2281 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcaggttaac
     2341 ctggcttatc gaaattaata cgactcacta tagggagacc ggcagatccg cggccgcata
     2401 ggccactagt ggatctgata tcatcgatga attcgagctc gtttaaactg gatggcggcg
     2461 ttagtatcga atcgacagca gtatagcgac cagcattcac atacgattga cgcatgatat
     2521 tactttctgc gcacttaact tcgcatctgg gcagatgatg tcgaggcgaa aaaaaatata
     2581 aatcacgcta acatttgatt aaaatagaac aactacaata taaaaaaact atacaaatga
     2641 caagttcttg aaaacaagaa tctttttatt gtcagtactt cacatcaaaa cacctttggt
     2701 tgagggaacg tcattggtgc cattgctaga aatagcttct cttatggcaa cagccaaagc
     2761 cttgaacgca ctctcacttc tgtggtgatc gttgaaacct ctcagacaat caacatgcaa
     2821 agtaattctg gccgcctccg cgaaactttc caaaaagtgt ggaatcattt cagtggataa
     2881 atcaccaatc atctctctct tcaatccaag gtcgattaca gcaaatggtc tactagataa
     2941 atcgactacg gcacgtgata gcgcctcatc caatggtgcg aacccagtac cgaatctttt
     3001 tacaccacgg acagcaccca ttgcttcttt gaacgcttgc cctaatgcga taccgcaatc
     3061 ttcggtagta tggtgatcgt caatgtgcag gtcaccaata cattcaacaa taagagacca
     3121 accagagtgt tttgccaacg catggatcat atgatccaaa aagccaacac ctgtgtgaat
     3181 atcgatgacc tgtgactgag tagcttggga agctacatcg tcatccttct ttgcaggaag
     3241 aatcgaatct tttatttgaa tataaccacc attcagcgaa atagcgattt gaattttagt
     3301 ttcattagtg atacgggaga taaacgcctt gcgctcctga acagtttgtt tttgcttttt
     3361 ttgggctggt tctgccatgg ttgtttatgt tcggatgtga tgtgagaact gtatcctagc
     3421 aagattttaa aaggaagtat atgaaagaag aacctcagtg gcaaatccta accttttata
     3481 tttctctaca ggggcgcggc gtggggacaa ttcaacgcgt ctgtgagggg agcgtttccc
     3541 tgctcgcagg tctgcagcga ggagccgtaa tttttgcttc gcgccgtgcg gccatcaaaa
     3601 tgtatggatg caaatgatta tacatgggga tgtatgggct aaatgtacgg gcgacagtca
     3661 catcatgccc ctgagctgcg cacgtcaaga ctgtcaagga gggtattctg ggcctccatg
     3721 tcgctggccg ggtgacccgg cggggacgag gcaagctaaa catagcattt tatatattct
     3781 gtttgtatta tttagtaatt gtatctagta catatcctgt agcggttggc ctgattcctt
     3841 ttaagttaga tgacaggcac actttgcatt atcacatcag acggaattgt tctgcctaat
     3901 ggcggctatt ctatttctgc ttcataaatg caaagacaga gtataagaat aattttaaat
     3961 taaaagcaat ggtcttatcc ttgccataaa cagaagacaa cttgcaagta aaacccggat
     4021 ctcaaaatgg cgtacgaagc ttcagctgg
//
