LOCUS       sequence-377876-        4221 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast gene induction with meiotic promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4221)
  AUTHORS   Dawson, D.
  TITLE     Dawson Lab Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4221)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pFA6a-HISMX6-pSPR3
FEATURES             Location/Qualifiers
     source          1..4221
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        492..835
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             836..1534
                     /codon_start=1
                     /product="S. kluyveri imidazoleglycerol phosphate 
                     dehydratase"
                     /label=k-HIS3
                     /translation="MAEPAQKKQKQTVQERKAFISRITNETKIQIAISLNGGYIQIKDS
                     ILPAKKDDDVASQATQSQVIDIHTGVGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHT
                     TEDCGIALGQAFKEAMGAVRGVKRFGTGFAPLDEALSRAVVDLSSRPFAVIDLGLKREM
                     IGDLSTEMIPHFLESFAEAARITLHVDCLRGFNDHHRSESAFKALAVAIREAISSNGTN
                     DVPSTKGVLM"
     terminator      1538..1735
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(1839..1858)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1840..1858)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1945..1962)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2116..2704)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2196..2215)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2875..3666,3667..3735))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3498..3517
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3736..3840)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3908..3926
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3964..3986)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4086..4105
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        4186..4204
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     4186..4203
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 agcttcgtac gccattttga gatccgggtt ttaataaaaa ttcaatgacg caaaaggaat
       61 tttaggtttt tattatttat ttatttattt ctgttaattg atccttttct ttccactacc
      121 aacaacaaaa aaggggggaa aaagatgtat aatctaaaag acactaatct gctcttgata
      181 tccttattat gtaatggaat aactcatata aatgtaaaat agaacttcaa attaatatta
      241 taatgatagt cgaggtcaga cacacttata atacattaag taaagaaaaa aaaatgtctg
      301 tcatcgaggt ctcttttgtg tcgctaacaa aacatcacta aatacgaaga cactttgcat
      361 gggaaggatg cagcaaatgg caaactaacg ggccattgat tggtttacct cttctatttg
      421 tattacgacc agaaagaacg aatggttttc atgtttagct tgcctcgtcc ccgccgggtc
      481 acccggccag cgacatggag gcccagaata ccctccttga cagtcttgac gtgcgcagct
      541 caggggcatg atgtgactgt cgcccgtaca tttagcccat acatccccat gtataatcat
      601 ttgcatccat acattttgat ggccgcacgg cgcgaagcaa aaattacggc tcctcgctgc
      661 agacctgcga gcagggaaac gctcccctca cagacgcgtt gaattgtccc cacgccgcgc
      721 ccctgtagag aaatataaaa ggttaggatt tgccactgag gttcttcttt catatacttc
      781 cttttaaaat cttgctagga tacagttctc acatcacatc cgaacataaa caaccatggc
      841 agaaccagcc caaaaaaagc aaaaacaaac tgttcaggag cgcaaggcgt ttatctcccg
      901 tatcactaat gaaactaaaa ttcaaatcgc tatttcgctg aatggtggtt atattcaaat
      961 aaaagattcg attcttcctg caaagaagga tgacgatgta gcttcccaag ctactcagtc
     1021 acaggtcatc gatattcaca caggtgttgg ctttttggat catatgatcc atgcgttggc
     1081 aaaacactct ggttggtctc ttattgttga atgtattggt gacctgcaca ttgacgatca
     1141 ccatactacc gaagattgcg gtatcgcatt agggcaagcg ttcaaagaag caatgggtgc
     1201 tgtccgtggt gtaaaaagat tcggtactgg gttcgcacca ttggatgagg cgctatcacg
     1261 tgccgtagtc gatttatcta gtagaccatt tgctgtaatc gaccttggat tgaagagaga
     1321 gatgattggt gatttatcca ctgaaatgat tccacacttt ttggaaagtt tcgcggaggc
     1381 ggccagaatt actttgcatg ttgattgtct gagaggtttc aacgatcacc acagaagtga
     1441 gagtgcgttc aaggctttgg ctgttgccat aagagaagct atttctagca atggcaccaa
     1501 tgacgttccc tcaaccaaag gtgttttgat gtgaagtact gacaataaaa agattcttgt
     1561 tttcaagaac ttgtcatttg tatagttttt ttatattgta gttgttctat tttaatcaaa
     1621 tgttagcgtg atttatattt tttttcgcct cgacatcatc tgcccagatg cgaagttaag
     1681 tgcgcagaaa gtaatatcat gcgtcaatcg tatgtgaatg ctggtcgcta tactgctgtc
     1741 gattcgatac taacgccgcc atccagttta aacgagctcg aattcatcga tgatatcaga
     1801 tccactagtg gcctatgcgg ccgcggatct gccggtctcc ctatagtgag tcgtattaat
     1861 ttcgataagc caggttaacc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     1921 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     1981 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     2041 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     2101 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     2161 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     2221 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     2281 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     2341 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     2401 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     2461 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     2521 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     2581 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     2641 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     2701 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     2761 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     2821 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     2881 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     2941 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     3001 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     3061 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     3121 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     3181 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     3241 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     3301 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     3361 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     3421 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     3481 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     3541 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     3601 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     3661 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     3721 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     3781 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     3841 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     3901 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga
     3961 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
     4021 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
     4081 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atggacatat tgtcgttaga
     4141 acgcggctac aattaataca taaccttatg tatcatacac atacgattta ggtgacacta
     4201 tagaacgcgg ccgccagctg a
//
