LOCUS       sequence-377877-        4802 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast gene induction with meiotic promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4802)
  AUTHORS   Dawson, D.
  TITLE     Dawson Lab Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pFA6a-HISMX6-pIME2
FEATURES             Location/Qualifiers
     source          1..4802
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1075..1418
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             1419..2117
                     /codon_start=1
                     /product="S. kluyveri imidazoleglycerol phosphate 
                     dehydratase"
                     /label=k-HIS3
                     /translation="MAEPAQKKQKQTVQERKAFISRITNETKIQIAISLNGGYIQIKDS
                     ILPAKKDDDVASQATQSQVIDIHTGVGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHT
                     TEDCGIALGQAFKEAMGAVRGVKRFGTGFAPLDEALSRAVVDLSSRPFAVIDLGLKREM
                     IGDLSTEMIPHFLESFAEAARITLHVDCLRGFNDHHRSESAFKALAVAIREAISSNGTN
                     DVPSTKGVLM"
     terminator      2121..2318
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(2422..2441)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2423..2441)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2528..2545)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2699..3287)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2779..2798)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3458..4249,4250..4318))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4081..4100
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4319..4423)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4491..4509
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4547..4569)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4669..4688
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        4769..4787
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     4769..4786
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 gaagcttcgt acgccatttt gagatccggg ttttaaatga cctattaagt taagcttagt
       61 actcttcttt tattacgtct gtatcacgtt ccttttcaat gaatatttaa gaacacgatt
      121 aacttttctg ataagcctgg taaataaata caggtttata ttctctagac agccacctta
      181 accgactgga gtgtccttta tatctttacc caaaaaataa aacttttagg agaaggggta
      241 acaaactgaa aagggaaagg gtaaagtaaa ggtaactgta aactattttg aaagatagat
      301 cttgtaactt ctttctcgtt gagtgatatt ggaaaataaa aatgaaatga atgtgtgaag
      361 acagaagaag ttattcagga aaagaaaatg gacagaccgg tgagttgcta ccttaatata
      421 tctatttgat tacacacatt acttctttac ctctttgcaa ccagcacttg tctgtggctt
      481 tttgccgccg aagtcccgcg ccttttccct gttgaattgc atttaagaca caaggctgac
      541 atttattaat gatagagatt tccacaaaaa ctcaaatagc cgccgtaaca taaattattg
      601 gacaaccgga gaaaaggata ttttaaggca ccagcgggta atacatcttt atttctccaa
      661 gtttaaaaag cgtatttgga aaagctgtgc cactcagatt tttgtgagag ttttcttttt
      721 ctacttacca tgagtgcgga gtcttggtga aacagctact gagagaatag gtatctgggt
      781 taccagtgta gttagctaca acatgttcga aatgcatggt agagtctggg tttcgttccc
      841 tttggcttgt taaggccgaa gattgctttg gtctggatgg gcggagccca aattttattg
      901 ttgaactatt tttagattaa gaaaaacagc gccaaatttt acccggctta tggctcctcc
      961 cttggtactc ccatttttat gacgtagcag ttgtcccgct tgtttcaact cctcgagaaa
     1021 agtggggcat tgggtgttta gcttgcctcg tccccgccgg gtcacccggc cagcgacatg
     1081 gaggcccaga ataccctcct tgacagtctt gacgtgcgca gctcaggggc atgatgtgac
     1141 tgtcgcccgt acatttagcc catacatccc catgtataat catttgcatc catacatttt
     1201 gatggccgca cggcgcgaag caaaaattac ggctcctcgc tgcagacctg cgagcaggga
     1261 aacgctcccc tcacagacgc gttgaattgt ccccacgccg cgcccctgta gagaaatata
     1321 aaaggttagg atttgccact gaggttcttc tttcatatac ttccttttaa aatcttgcta
     1381 ggatacagtt ctcacatcac atccgaacat aaacaaccat ggcagaacca gcccaaaaaa
     1441 agcaaaaaca aactgttcag gagcgcaagg cgtttatctc ccgtatcact aatgaaacta
     1501 aaattcaaat cgctatttcg ctgaatggtg gttatattca aataaaagat tcgattcttc
     1561 ctgcaaagaa ggatgacgat gtagcttccc aagctactca gtcacaggtc atcgatattc
     1621 acacaggtgt tggctttttg gatcatatga tccatgcgtt ggcaaaacac tctggttggt
     1681 ctcttattgt tgaatgtatt ggtgacctgc acattgacga tcaccatact accgaagatt
     1741 gcggtatcgc attagggcaa gcgttcaaag aagcaatggg tgctgtccgt ggtgtaaaaa
     1801 gattcggtac tgggttcgca ccattggatg aggcgctatc acgtgccgta gtcgatttat
     1861 ctagtagacc atttgctgta atcgaccttg gattgaagag agagatgatt ggtgatttat
     1921 ccactgaaat gattccacac tttttggaaa gtttcgcgga ggcggccaga attactttgc
     1981 atgttgattg tctgagaggt ttcaacgatc accacagaag tgagagtgcg ttcaaggctt
     2041 tggctgttgc cataagagaa gctatttcta gcaatggcac caatgacgtt ccctcaacca
     2101 aaggtgtttt gatgtgaagt actgacaata aaaagattct tgttttcaag aacttgtcat
     2161 ttgtatagtt tttttatatt gtagttgttc tattttaatc aaatgttagc gtgatttata
     2221 ttttttttcg cctcgacatc atctgcccag atgcgaagtt aagtgcgcag aaagtaatat
     2281 catgcgtcaa tcgtatgtga atgctggtcg ctatactgct gtcgattcga tactaacgcc
     2341 gccatccagt ttaaacgagc tcgaattcat cgatgatatc agatccacta gtggcctatg
     2401 cggccgcgga tctgccggtc tccctatagt gagtcgtatt aatttcgata agccaggtta
     2461 acctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     2521 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     2581 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     2641 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     2701 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     2761 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     2821 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     2881 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     2941 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     3001 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     3061 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     3121 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     3181 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     3241 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     3301 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     3361 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     3421 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     3481 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     3541 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     3601 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     3661 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     3721 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     3781 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     3841 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     3901 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     3961 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     4021 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     4081 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     4141 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     4201 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     4261 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     4321 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     4381 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     4441 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     4501 tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
     4561 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
     4621 agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc
     4681 agattgtact gagagtgcac catatggaca tattgtcgtt agaacgcggc tacaattaat
     4741 acataacctt atgtatcata cacatacgat ttaggtgaca ctatagaacg cggccgccag
     4801 ct
//
