LOCUS       sequence-377878-        3966 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast gene induction with copper promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3966)
  AUTHORS   Dawson, D.
  TITLE     Dawson Lab Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pFA6a-TRP1-pCUP1-2xphlO
FEATURES             Location/Qualifiers
     source          1..3966
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        623..770
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             771..1445
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     RBS             1525..1533
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     primer_bind     complement(1605..1624)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1606..1624)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1711..1728)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1882..2470)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1962..1981)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2641..3432,3433..3501))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3264..3283
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3502..3606)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3674..3692
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3730..3752)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3852..3871
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        join(3952..3966,1..4)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     join(3952..3966,1..3)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 tagaacgcgg ccgccagctg aagcttcgta cgctgcaggt cgacggatcc ccgggttaat
       61 taaagacatt ttgagatccg ggttttttta tgtgatgatt gattgattga ttgtacagtt
      121 tgtttttctt aatatctatt tcgatgactt ctatatgata gtgcacctta acgatacggt
      181 acgtttcgta tcatacatgc ttaaaagtca taaaatttta accttaacga tacggtacgt
      241 ttcgtatcat acatagatac aagacaagga gttatttgct tctcttttat atgattctga
      301 caatccatat tgcgttggta gtcttttttg ctggaacggt tcagcggaaa agacgcatcg
      361 ctctttttgc ttctagaaga aatgccagca aaagaatctc ttgacagtga ctgacagcaa
      421 aaatgtcttt ttctaactag taacaaggct aagatatcag tccgaaataa agggtggtga
      481 agtaataatt aaatcatccg tataaaccta tacacatata tgaggaaaaa taatacaaaa
      541 gtgttttaaa tacagataca tacatgaaca tatgcacgta tagcgcccaa atgtcggtaa
      601 tgggatcggc ttactaggat ctgtacaatc ttgatccgga gcttttcttt ttttgccgat
      661 taagaattaa ttcggtcgaa aaaagaaaag gagagggcca agagggaggg cattggtgac
      721 tattgagcac gtgagtatac gtgattaagc acacaaaggc agcttggagt atgtctgtta
      781 ttaatttcac aggtagttct ggtccattgg tgaaagtttg cggcttgcag agcacagagg
      841 ccgcagaatg tgctctagat tccgatgctg acttgctggg tattatatgt gtgcccaata
      901 gaaagagaac aattgacccg gttattgcaa ggaaaatttc aagtcttgta aaagcatata
      961 aaaatagttc aggcactccg aaatacttgg ttggcgtgtt tcgtaatcaa cctaaggagg
     1021 atgttttggc tctggtcaat gattacggca ttgatatcgt ccaactgcat ggagatgagt
     1081 cgtggcaaga ataccaagag ttcctcggtt tgccagttat taaaagactc gtatttccaa
     1141 aagactgcaa catactactc agtgcagctt cacagaaacc tcattcgttt attcccttgt
     1201 ttgattcaga agcaggtggg acaggtgaac ttttggattg gaactcgatt tctgactggg
     1261 ttggaaggca agagagcccc gaaagcttac attttatgtt agctggtgga ctgacgccag
     1321 aaaatgttgg tgatgcgctt agattaaatg gcgttattgg tgttgatgta agcggaggtg
     1381 tggagacaaa tggtgtaaaa gactctaaca aaatagcaaa tttcgtcaaa aatgctaaga
     1441 aataggttat tactgagtag tatttattta agtattgttt gtgcacttgc cgatctatgc
     1501 ggtgtgaaat accgcacaga tgcgtaagga ggtttaaacg agctcgaatt catcgatgat
     1561 atcagatcca ctagtggcct atgcggccgc ggatctgccg gtctccctat agtgagtcgt
     1621 attaatttcg ataagccagg ttaacctgca ttaatgaatc ggccaacgcg cggggagagg
     1681 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     1741 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     1801 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     1861 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     1921 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     1981 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     2041 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     2101 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     2161 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     2221 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     2281 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     2341 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     2401 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     2461 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     2521 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     2581 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     2641 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     2701 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     2761 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     2821 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     2881 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     2941 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     3001 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     3061 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     3121 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     3181 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     3241 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     3301 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     3361 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     3421 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     3481 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     3541 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     3601 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     3661 attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga
     3721 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
     3781 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg
     3841 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgg acatattgtc
     3901 gttagaacgc ggctacaatt aatacataac cttatgtatc atacacatac gatttaggtg
     3961 acacta
//
