LOCUS       sequence-377886-        9608 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses HiFiCas9-POLB (HiFi iFE).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9608)
  AUTHORS   Nakade S, Nakamae K, Tang T-C, Yu D, Sakuma T, Yamamoto T, Lu TK
  TITLE     Frame Editors for Precise, Template-Free Frameshifting
  JOURNAL   bioRxiv 2022.12.05.518807
REFERENCE   2  (bases 1 to 9608)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2022.12.05.518807"
COMMENT     Sequence Label: HiFi iFE
FEATURES             Location/Qualifiers
     source          1..9608
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        9..294
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     promoter        296..573
                     /label=chicken beta-actin promoter
     intron          574..801
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      813..822
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             822..887
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             894..914
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             939..5039
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANANFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5040..5087
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             6135..6155
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(6178..6195)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6184..6391
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   6400..6540
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   6400..6529
                     /label=AAV2 ITR
     rep_origin      6615..7070
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6702..6721)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6912..6933
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7087..7106)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7206..7228
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7266..7284)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7352..7456
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7457..7525,7526..8317)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7675..7694)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8488..9076
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8977..8996
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9151..9456)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGAGL"
     primer_bind     9306..9328
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9320..9337
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9321..9337
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9347..9366
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9347..9365
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9413..9433)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9413..9431)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9452..9468)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9452..9468)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9465..9487)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9476..9492
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9500..9506,9507..9524,9525..9530))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9545..9566
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gaggtacccg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
       61 cgcccattga cgtcaatagt aacgccaata gggactttcc attgacgtca atgggtggag
      121 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
      181 cctattgacg tcaatgacgg taaatggccc gcctggcatt gtgcccagta catgacctta
      241 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtcgag
      301 gtgagcccca cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg
      361 tatttattta ttttttaatt attttgtgca gcgatggggg cggggggggg gggggggcgc
      421 gcgccrggcg gggcggggsg gggsgrgggg sggggsgggg sgrggcggag aggtgcggcg
      481 gcagccaatc agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg
      541 cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgcgctg ccttcgcccc
      601 gtgccccgct ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac cgcgttactc
      661 ccacaggtga gcgggcggga cggcccttct cctccgggct gtaattagct gagcaagagg
      721 taagggttta agggatggtt ggttggtggg gtattaatgt ttaattacct ggagcacctg
      781 cctgaaatca ctttttttca ggttggaccg gtgccaccat ggactataag gaccacgacg
      841 gagactacaa ggatcatgat attgattaca aagacgatga cgataagatg gccccaaaga
      901 agaagcggaa ggtcggtatc cacggagtcc cagcagccga caagaagtac agcatcggcc
      961 tggacatcgg caccaactct gtgggctggg ccgtgatcac cgacgagtac aaggtgccca
     1021 gcaagaaatt caaggtgctg ggcaacaccg accggcacag catcaagaag aacctgatcg
     1081 gagccctgct gttcgacagc ggcgaaacag ccgaggccac ccggctgaag agaaccgcca
     1141 gaagaagata caccagacgg aagaaccgga tctgctatct gcaagagatc ttcagcaacg
     1201 agatggccaa ggtggacgac agcttcttcc acagactgga agagtccttc ctggtggaag
     1261 aggataagaa gcacgagcgg caccccatct tcggcaacat cgtggacgag gtggcctacc
     1321 acgagaagta ccccaccatc taccacctga gaaagaaact ggtggacagc accgacaagg
     1381 ccgacctgcg gctgatctat ctggccctgg cccacatgat caagttccgg ggccacttcc
     1441 tgatcgaggg cgacctgaac cccgacaaca gcgacgtgga caagctgttc atccagctgg
     1501 tgcagaccta caaccagctg ttcgaggaaa accccatcaa cgccagcggc gtggacgcca
     1561 aggccatcct gtctgccaga ctgagcaaga gcagacggct ggaaaatctg atcgcccagc
     1621 tgcccggcga gaagaagaat ggcctgttcg gaaacctgat tgccctgagc ctgggcctga
     1681 cccccaactt caagagcaac ttcgacctgg ccgaggatgc caaactgcag ctgagcaagg
     1741 acacctacga cgacgacctg gacaacctgc tggcccagat cggcgaccag tacgccgacc
     1801 tgtttctggc cgccaagaac ctgtccgacg ccatcctgct gagcgacatc ctgagagtga
     1861 acaccgagat caccaaggcc cccctgagcg cctctatgat caagagatac gacgagcacc
     1921 accaggacct gaccctgctg aaagctctcg tgcggcagca gctgcctgag aagtacaaag
     1981 agattttctt cgaccagagc aagaacggct acgccggcta cattgacggc ggagccagcc
     2041 aggaagagtt ctacaagttc atcaagccca tcctggaaaa gatggacggc accgaggaac
     2101 tgctcgtgaa gctgaacaga gaggacctgc tgcggaagca gcggaccttc gacaacggca
     2161 gcatccccca ccagatccac ctgggagagc tgcacgccat tctgcggcgg caggaagatt
     2221 tttacccatt cctgaaggac aaccgggaaa agatcgagaa gatcctgacc ttccgcatcc
     2281 cctactacgt gggccctctg gccaggggaa acagcagatt cgcctggatg accagaaaga
     2341 gcgaggaaac catcaccccc tggaacttcg aggaagtggt ggacaagggc gcttccgccc
     2401 agagcttcat cgagcggatg accaacttcg ataagaacct gcccaacgag aaggtgctgc
     2461 ccaagcacag cctgctgtac gagtacttca ccgtgtataa cgagctgacc aaagtgaaat
     2521 acgtgaccga gggaatgaga aagcccgcct tcctgagcgg cgagcagaaa aaggccatcg
     2581 tggacctgct gttcaagacc aaccggaaag tgaccgtgaa gcagctgaaa gaggactact
     2641 tcaagaaaat cgagtgcttc gactccgtgg aaatctccgg cgtggaagat cggttcaacg
     2701 cctccctggg cacataccac gatctgctga aaattatcaa ggacaaggac ttcctggaca
     2761 atgaggaaaa cgaggacatt ctggaagata tcgtgctgac cctgacactg tttgaggaca
     2821 gagagatgat cgaggaacgg ctgaaaacct atgcccacct gttcgacgac aaagtgatga
     2881 agcagctgaa gcggcggaga tacaccggct ggggcaggct gagccggaag ctgatcaacg
     2941 gcatccggga caagcagtcc ggcaagacaa tcctggattt cctgaagtcc gacggcttcg
     3001 ccaacgccaa cttcatgcag ctgatccacg acgacagcct gacctttaaa gaggacatcc
     3061 agaaagccca ggtgtccggc cagggcgata gcctgcacga gcacattgcc aatctggccg
     3121 gcagccccgc cattaagaag ggcatcctgc agacagtgaa ggtggtggac gagctcgtga
     3181 aagtgatggg ccggcacaag cccgagaaca tcgtgatcga aatggccaga gagaaccaga
     3241 ccacccagaa gggacagaag aacagccgcg agagaatgaa gcggatcgaa gagggcatca
     3301 aagagctggg cagccagatc ctgaaagaac accccgtgga aaacacccag ctgcagaacg
     3361 agaagctgta cctgtactac ctgcagaatg ggcgggatat gtacgtggac caggaactgg
     3421 acatcaaccg gctgtccgac tacgatgtgg accatatcgt gcctcagagc tttctgaagg
     3481 acgactccat cgacaacaag gtgctgacca gaagcgacaa gaaccggggc aagagcgaca
     3541 acgtgccctc cgaagaggtc gtgaagaaga tgaagaacta ctggcggcag ctgctgaacg
     3601 ccaagctgat tacccagaga aagttcgaca atctgaccaa ggccgagaga ggcggcctga
     3661 gcgaactgga taaggccggc ttcatcaaga gacagctggt ggaaacccgg cagatcacaa
     3721 agcacgtggc acagatcctg gactcccgga tgaacactaa gtacgacgag aatgacaagc
     3781 tgatccggga agtgaaagtg atcaccctga agtccaagct ggtgtccgat ttccggaagg
     3841 atttccagtt ttacaaagtg cgcgagatca acaactacca ccacgcccac gacgcctacc
     3901 tgaacgccgt cgtgggaacc gccctgatca aaaagtaccc taagctggaa agcgagttcg
     3961 tgtacggcga ctacaaggtg tacgacgtgc ggaagatgat cgccaagagc gagcaggaaa
     4021 tcggcaaggc taccgccaag tacttcttct acagcaacat catgaacttt ttcaagaccg
     4081 agattaccct ggccaacggc gagatccgga agcggcctct gatcgagaca aacggcgaaa
     4141 ccggggagat cgtgtgggat aagggccggg attttgccac cgtgcggaaa gtgctgagca
     4201 tgccccaagt gaatatcgtg aaaaagaccg aggtgcagac aggcggcttc agcaaagagt
     4261 ctatcctgcc caagaggaac agcgataagc tgatcgccag aaagaaggac tgggacccta
     4321 agaagtacgg cggcttcgac agccccaccg tggcctattc tgtgctggtg gtggccaaag
     4381 tggaaaaggg caagtccaag aaactgaaga gtgtgaaaga gctgctgggg atcaccatca
     4441 tggaaagaag cagcttcgag aagaatccca tcgactttct ggaagccaag ggctacaaag
     4501 aagtgaaaaa ggacctgatc atcaagctgc ctaagtactc cctgttcgag ctggaaaacg
     4561 gccggaagag aatgctggcc tctgccggcg aactgcagaa gggaaacgaa ctggccctgc
     4621 cctccaaata tgtgaacttc ctgtacctgg ccagccacta tgagaagctg aagggctccc
     4681 ccgaggataa tgagcagaaa cagctgtttg tggaacagca caagcactac ctggacgaga
     4741 tcatcgagca gatcagcgag ttctccaaga gagtgatcct ggccgacgct aatctggaca
     4801 aagtgctgtc cgcctacaac aagcaccggg ataagcccat cagagagcag gccgagaata
     4861 tcatccacct gtttaccctg accaatctgg gagcccctgc cgccttcaag tactttgaca
     4921 ccaccatcga ccggaagagg tacaccagca ccaaagaggt gctggacgcc accctgatcc
     4981 accagagcat caccggcctg tacgagacac ggatcgacct gtctcagctg ggaggcgaca
     5041 aaaggccggc ggccacgaaa aaggccggcc aggcaaaaaa gaaaaagggc ggcggatctg
     5101 gtggtgggtc tggcggaggc tcgtctaagc gaaaggctcc gcaggagacc cttaacggtg
     5161 gcatcacgga tatgctgacg gagctggcta attttgaaaa aaatgtttca caagctatcc
     5221 acaagtacaa cgcctacaga aaagctgctt ctgtaatagc caaatatcct cataaaatca
     5281 aatcaggggc cgaggccaaa aaactgccgg gggtggggac gaaaatcgca gaaaaaatag
     5341 atgagttttt ggcgacgggt aaattgcgaa agctggaaaa gatcaggcag gacgacacct
     5401 cttctagtat taatttcctc acgagagtaa gcggtatcgg acctagtgcc gccaggaagt
     5461 tcgtagatga ggggattaag acattggaag atctccggaa gaacgaggac aagttgaatc
     5521 atcaccagcg aataggactt aaatatttcg gagacttcga aaagcgcatt ccacgagagg
     5581 agatgctgca aatgcaagac atagtgctta atgaagtcaa aaaagtagac agcgagtaca
     5641 tagctactgt ctgcggatct tttaggcggg gtgctgagtc ttctggtgat atggatgtac
     5701 tgctgactca tccatctttt acaagcgaga gcactaagca accaaaactg cttcaccaag
     5761 tcgtcgagca actgcagaag gtacacttta tcactgacac tctgtctaaa ggtgaaacga
     5821 agttcatggg cgtctgccag ttgccgagta aaaatgatga aaaagaatat ccgcatcgga
     5881 gaatagacat ccgactcatc ccgaaggacc aatactactg tggggtcttg tacttcacgg
     5941 gctcagacat tttcaacaag aatatgaggg cacacgccct ggaaaagggt tttacaataa
     6001 atgagtacac gatcagacct ctgggagtca ccggggttgc gggagagccg cttccggttg
     6061 actcagaaaa ggacattttc gactatatcc agtggaaata cagagaacca aaggacagaa
     6121 gtgagggtgg ttctccaaag aagaagcgga aggtctagcc tagagctcgc tgatcagcct
     6181 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     6241 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     6301 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     6361 attgggaaga gaatagcagg catgctgggg agcggccgca ggaaccccta gtgatggagt
     6421 tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc
     6481 gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg
     6541 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc
     6601 aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     6661 gcgcagcgtg accgctacac ttgccagcgc cttagcgccc gctcctttcg ctttcttccc
     6721 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     6781 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg
     6841 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     6901 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaactcta tctcgggcta
     6961 ttcttttgat ttataaggga ttttgccgat ttcggtctat tggttaaaaa atgagctgat
     7021 ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac
     7081 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
     7141 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     7201 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
     7261 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     7321 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     7381 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     7441 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     7501 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     7561 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     7621 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     7681 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     7741 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     7801 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     7861 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     7921 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     7981 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     8041 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     8101 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     8161 cggtgagcgt ggaagccgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     8221 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     8281 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     8341 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     8401 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     8461 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     8521 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     8581 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     8641 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     8701 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     8761 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     8821 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     8881 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     8941 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     9001 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     9061 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     9121 gccttttgct cacatgtcag acaaatggct ctagagaccg gcgccgctac agggcgcgtc
     9181 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     9241 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     9301 gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat acgactcact
     9361 atagggcgaa ttgggtaccg ggccccccct cgaggtcctc cagcttttgt tccctttagt
     9421 gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     9481 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     9541 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccaccggt
     9601 ggtctcta
//
