LOCUS       sequence-377906-        9073 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Barcoded piggybac transposon vector with Dox-inducible expression of
            TAF4B.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9073)
  AUTHORS   Pierson Smela MD, Kramme CC, Fortuna PRJ, Adams JL, Su R, Dong E, 
            Kobayashi M, Brixi G, Kavirayuni VS, Tysinger E, Kohman RE, Shioda 
            T, Chatterjee P, Church GM
  TITLE     Directed differentiation of human iPSCs to functional ovarian 
            granulosa-like cells via transcription factor overexpression.
  JOURNAL   Elife. 2023 Feb 21;12:e83291. doi: 10.7554/eLife.83291.
  PUBMED    36803359
REFERENCE   2  (bases 1 to 9073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2023-02-21"; volume: "12:e83291. doi"; pages: " 
            10.7554/eLife.83291"
COMMENT     Sequence Label: pPB-cT3G-cERP2-TAF4B
FEATURES             Location/Qualifiers
     source          1..9073
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     21..45
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    111..135
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    1975..1986
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     protein_bind    complement(2731..2755)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2757..2783
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    2950..3084
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   3356..3390
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(3401..3418)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3401..3417)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3410..3432)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(3626..3645)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3747..3769
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3808..3826)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3894..3998
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3999..4067,4068..4859)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4217..4236)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5030..5618
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5519..5538
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5772..5789
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5995..6016
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6031..6036,6037..6054,6055..6061)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6069..6085
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6074..6096
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6093..6109
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6093..6109
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   6135..6197
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    6373..6454
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(7171..7227)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(7237..7980)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(7981..8004)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      8004..8013
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(8033..8244)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     enhancer        8245..8624
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     protein_bind    8833..8851
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8869..8887
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8905..8923
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8941..8959
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8977..8995
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9013..9031
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9049..9067
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 aagcttgcct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
       61 ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctacc acaagtttgt
      121 acaaaaaagc aggctgccac catgcccgcc ggcctcaccg aacccgccgg cgccgctccc
      181 ccggctgctg tgagcgcctc ggggaccgtg accatggccc cggccggggc gctgccggtg
      241 cgggtggaga gcactccggt ggccctgggc gccgtgacta aggctcctgt cagcgtctgc
      301 gtggagccca cggcgtccca gcccctgcgg tcccccgtgg ggaccctggt gaccaaagtg
      361 gctccggtca gcgcccctcc taaagtcagc agcggcccta ggctgcctgc tcctcagata
      421 gtcgccgtga aagcccccaa caccacgaca atccagtttc ctgctaattt gcagcttcct
      481 ccaggaaccg ttttgattaa aagtaacagt ggtccgttga tgttggtatc tcctcagcaa
      541 actgtaacaa gagccgagac cacaagtaac ataacctcaa ggccagcagt accagcgaat
      601 cctcaaacag tcaaaatctg tacagtgccg aactctagct cacaattaat caagaaagtg
      661 gcagtgacac ctgttaaaaa attggcacaa ataggaacta ctgtggtaac cactgttccg
      721 aagccttcct cagtacaatc tgtggctgtg ccaaccagtg tcgtcacagt tactcctgga
      781 aagccattga atactgtaac taccctgaag ccttcaagtt tgggagcatc atccactcct
      841 tcaaatgagc ccaatcttaa agcagagaac tcagcagctg ttcagattaa tctttctccg
      901 acaatgctag aaaatgtgaa gaaatgcaag aacttccttg caatgttaat aaaactagca
      961 tgtagtggat cacagtcccc agaaatgggg caaaatgtga agaagctggt ggaacaactt
     1021 ttggatgcaa aaatcgaagc agaagaattt actaggaaac tgtatgttga actcaagtct
     1081 tcacctcagc ctcacctggt tccttttctt aagaaaagcg tggttgcctt acgacaactt
     1141 ctgcctaact cccagagctt catccagcaa tgtgttcagc agacttctag tgacatggtc
     1201 attgctacct gtactacaac agtaacaact tctcctgtgg tgacaactac agtgtcctca
     1261 agccagtctg aaaagtcaat tattgtttct ggagcaacag cacccagaac tgtgtcagtg
     1321 caaactttga acccacttgc tggtccagtg ggagcaaaag ctggagttgt gacacttcat
     1381 tctgtgggcc caactgctgc aacaggagga acaacagctg gaactggttt gcttcagact
     1441 tcaaaaccac ttgtgacatc tgtggcaaac acagtgacca cggtctcact gcaacctgaa
     1501 aagccagttg tctctggaac agcagtaaca ctgtcccttc cagcagtaac ttttggagaa
     1561 acttcaggtg cagctatttg tcttccatct gtgaaacctg ttgtttcttc tgctgggacc
     1621 acatctgaca agcctgttat tgggactcca gttcaaatca aacttgccca gccgggccct
     1681 gtcctttcac aaccagctgg gattccacag gcagttcaag tcaagcaact agtagttcag
     1741 cagccttcag gaggcaatga aaaacaagtg accacaattt cacattcctc aacattgacc
     1801 attcagaaat gtggacagaa gacgatgcca gtgaacacca taatacctac tagtcagttt
     1861 cctccagctt ccattctaaa gcaaattact ctgcctggaa ataaaattct gtcacttcaa
     1921 gcatctccta ctcagaaaaa cagaataaaa gagaatgtaa catcatgctt ccgagatgag
     1981 gatgacatca atgatgtgac ttctatggca ggggtcaacc ttaatgaaga aaatgcctgc
     2041 atcttagcaa caaactctga attggttggc acactcattc agtcatgtaa agatgaacca
     2101 tttcttttta ttggagctct acaaaagaga attttagaca ttggtaaaaa gcatgacatt
     2161 acagaactta actctgatgc tgtgaacttg atctcccaag caacacagga acgactacga
     2221 ggccttctag aaaaactgac tgcaattgct cagcatcgaa tgactactta caaggcaagt
     2281 gaaaattaca tcctgtgtag tgataccagg tcacagctca aatttcttga aaagctggat
     2341 caattggaga agcagagaaa ggatttggaa gaaagagaaa tgttacttaa ggcagccaag
     2401 agtcgttcta ataaagaaga tccagaacag ctgagattaa agcagaaagc caaagagtta
     2461 cagcaattgg aacttgcaca gatacagcat agagacgcta atctcacagc tcttgcagct
     2521 attggaccaa ggaagaagag accactagaa tctggaattg agggcttaaa agacaacctt
     2581 cttgcttctg ggacatccag cctgacagcc accaaacagt tgcatcgtcc aagaatcacg
     2641 agaatctgcc tcagggactt gatattttgt atggaacagg aacgggagat gaagtattct
     2701 cgagctctat acctggccct tctgaagtga acccagcttt cttgtacaaa gtggtatacc
     2761 catacgatgt tccagattac gcttagtaat gagaaatact cagaagatgt cactcaccat
     2821 agtccgcagt gaaagataaa tgatcttctt caatgctggg tgatagtttt agagctagaa
     2881 atagcaagta ggccactgag caagaactat gaatgagatc tttagacata ttgcggccgc
     2941 tttctccgcg atccagacat gataagatac attgatgagt ttggacaaac caaaactaga
     3001 atgcagtgaa aaaaatgcct tatttgtgaa atttgtgatg ctattgcctt atttgtaacc
     3061 attataagct gcaataaaca agttatctat aacaagaaaa tatatatata ataagttatc
     3121 acgtaagtag aacatgaaat aacaatataa ttatcgtatg agttaaatct taaaagtcac
     3181 gtaaaagata atcatgcgtc attttgactc acgcggtcgt tatagttcaa aatcagtgac
     3241 acttaccgca ttgacaagca cgcctcacgg gagctccaag cggcgactga gatgtcctaa
     3301 atgcacagcg acggattcgc gctatttaga aagagagagc aatatttcaa gaatgcatgc
     3361 gtcaatttta cgcagactat ctttctaggg ttaagaattc actggccgtc gttttacaac
     3421 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     3481 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     3541 gcctgaatgg cgaatggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     3601 cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
     3661 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     3721 cttacagaca agctgtgacc atgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     3781 atcaccgaaa cacgcgagtt cgaaagggcc tcgtgatacg cctattttta taggttaatg
     3841 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     3901 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     3961 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     4021 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     4081 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     4141 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     4201 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     4261 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     4321 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     4381 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     4441 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     4501 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     4561 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     4621 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     4681 ttattgctga taaatctgga gccggtgagc gtggttcacg cggtatcatt gcagcactgg
     4741 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     4801 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     4861 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     4921 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     4981 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     5041 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     5101 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     5161 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     5221 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     5281 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     5341 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     5401 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     5461 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     5521 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     5581 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     5641 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     5701 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     5761 cgaccgagcg cagcgagtca gtgagcgagg aagcggatga gcgcccaata cgcaaaccgc
     5821 ctctcaccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     5881 aagcgggcag tgagcgcaac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     5941 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     6001 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     6061 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
     6121 caaggtcgac ttaaccctag aaagataatc atattgtgac gtacgttaaa gataatcatg
     6181 cgtaaaattg acgcatgtgt tttatcggtc tgtatatcga ggtttattta ttaatttgaa
     6241 tagatattaa gttttattat atttacactt acatactaat aataaattca acaaacaatt
     6301 tatttatgtt tatttattta ttaaaaaaaa acaaaaactc aaaatttctt ctataaagta
     6361 acaaaacttt tagcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
     6421 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
     6481 gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg
     6541 tggtatggct gattatgatc ctctggagat tcatgcgcca ggttttctgg tcatgcacca
     6601 cgttcgtggg ccctcaggaa cctccacgtc agcggtaact gtgaagccca gtcgctcgta
     6661 aaaaggaaga ttccggggag cactggtttc gaggaacgcc ggcacgccag cccgttccgc
     6721 ggcctcaaca ccaggaagaa cgacagcgct tcccaaacct ttaccttggt gatctgggct
     6781 gacaccgaca gtggcaagaa accacgcagg ctcctttggt ctatgaggag ccagcaagcc
     6841 ctccatttgc tgttgtgccg ccaacctact ccctgacagc tcggccatcc gcggacctat
     6901 ttccgcgaac actgcgccgg cttcgacgct ttccggagta gtccagaccg caactgcggc
     6961 accatcgtcc gcaacccaaa ctttaccaat gtccagaccc acgcgcgtaa gaaacagctc
     7021 ttgcaactca gttaccctct ctatgtgtct gtccggatct acggtatggc gggtcgctgg
     7081 ataatctgca aatgctgcgg caagtgttcg gacagcccgt ggtacatcgt cacgggtagc
     7141 gagcctaaca gtgggcttat attccgtcat ggggccgggg ttctcctcca cgtcgccggc
     7201 ctgcttcagc aggctgaagt tggtggcgcc gctgcccccg gggagcatgt caaggtcaaa
     7261 atcgtcaaga gcgtcagcag gcagcatatc aaggtcaaag tcgtcaaggg catcggctgg
     7321 gagcatgtct aagtcaaaat cgtcaagggc gtcggtcggc ccgccgcttt cgcactttag
     7381 ctgtttctcc aggccacata tgattagttc caggccgaaa aggaaggcag gttcggctcc
     7441 ctgccggtcg aacagctcaa ttgcttgttt cagaagtggg ggcatagaat cggtggtagg
     7501 tgtctctctt tcctcttttg ctacttgatg ctcctgttcc tccaatacgc agcccagtgt
     7561 aaagtggccc acggcggaca gagcgtacag tgcgttctcc agggagaagc cttgctgaca
     7621 caggaacgcg agctgatttt ccagggtttc gtactgtttc tctgttgggc gggtgccgag
     7681 atgcacttta gccccgtcac gatgtgagag gagagcacag cggtatgact tggcgttgtt
     7741 ccgcagaaag tcttgccatg actcgccttc cagggggcag gagtgggtat gatgcctgtc
     7801 cagcatctcg attggcaggg catcgagcag ggcccgcttg ttcttcacgt gccagtacag
     7861 ggtaggctgc tcaactccca gcttttgagc gagtttcctt gtcgtcaggc cttcgatacc
     7921 gactccattg agtaattcca gagcagagtt tatgactttg ctcttgtcca gtctagacat
     7981 cttatcgtca tcgtctttgt aatccatggt ggcggatccc gcgtcacgac acctgtgttc
     8041 tggcggcaaa cccgttgcga aaaagaacgt tcacggcgac tactgcactt atatacggtt
     8101 ctcccccacc ctcgggaaaa aggcggagcc agtacacgac atcactttcc cagtttaccc
     8161 cgcgccacct tctctaggca ccggttcaat tgccgacccc tccccccaac ttctcgggca
     8221 ctgtgggcca tgtgcgctct gccccatggt aatagccatg actaatacgt agatgtactg
     8281 ccaagtagga aagtgccata aggtcatgta ctgggcataa tgccaggcgg gccatttacc
     8341 gtcattgacg tcaatagggg gcgtacttgg catatgatac acttgatgta ctgccaagtg
     8401 ggcagtttac cgtaaatact ccacccattg acgtcaatgg aaagtgccta ttggcgttac
     8461 tatgggaaca tacgtcatta ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg
     8521 cgggccattt accgtaagtt atgtaacgcg gaactccata tatgggctat gaactaatga
     8581 ccccgtaatt gattactatt aataactagt caataatcaa tgtcataagc tctaatcgca
     8641 taatcgagct aagagcgatt aatatgctta gctagcttag ctaagctaga tttctctatt
     8701 ctcccacaat atatagcagc tattagctta gcagtccgaa tacgatcgtc cacgatccat
     8761 aagcgtaaca catttgagta gcgactgacg ggcaccggag ccactcgagt ggaattatca
     8821 cctcgagttt actccctatc agtgatagag aacgtatgaa gagtttactc cctatcagtg
     8881 atagagaacg tatgcagact ttactcccta tcagtgatag agaacgtata aggagtttac
     8941 tccctatcag tgatagagaa cgtatgacca gtttactccc tatcagtgat agagaacgta
     9001 tctacagttt actccctatc agtgatagag aacgtatatc cagtttactc cctatcagtg
     9061 atagagaacg tat
//
