LOCUS       sequence-37792-e         878 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Certo MT, Ryu BY, Annis JE, Garibov M, Jarjour J, Rawlings DJ, 
            Scharenberg AM
  TITLE     Tracking genome engineering outcome at individual DNA breakpoints.
  JOURNAL   Nat Methods. 2011 Jul 10;8(8):671-6. doi: 10.1038/nmeth.1648.
  PUBMED    21743461
REFERENCE   2  (bases 1 to 878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-07-10- 10"; volume: "8"; 
            issue: "8"; pages: "671-6"
COMMENT     Sequence Label: pRRL sEF1a HA.NLS.Sce(opt).T2A.IFP
FEATURES             Location/Qualifiers
     source          1..878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             8..34
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             8..34
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             44..64
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             785..838
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 gggcgtatac ccctacgacg tgcccgacta cgcccccggg ccccctaaga aaaagaggaa
       61 ggtgaagaac atcaagaaga accaggtcat gaacctgggc cccaacagca agctgctgaa
      121 ggagtacaag agccagctga tcgagctgaa catcgagcag ttcgaggccg gcatcggcct
      181 gatcctgggc gacgcctaca tcaggagcag ggacgagggc aagacctact gcatgcagtt
      241 cgagtggaag aacaaggcct acatggacca cgtgtgcctg ctgtacgacc agtgggtgct
      301 gagccccccc cacaagaagg agagggtgaa ccacctgggc aacctggtca tcacctgggg
      361 cgcccagacc ttcaagcacc aggccttcaa caagctggcc aacctgttca tcgtgaacaa
      421 caagaagacc atccccaaca acctggtgga gaactacctg acccccatga gcctggccta
      481 ctggttcatg gacgacggcg gcaagtggga ctacaacaag aacagcacca acaagagcat
      541 cgtgctgaac acccagagct tcaccttcga ggaggtggag tacctggtga agggcctgag
      601 gaacaagttc cagctgaact gctacgtgaa gatcaacaag aacaagccca tcatctacat
      661 cgacagcatg agctacctga tcttctacaa cctgatcaag ccctacctga tcccccagat
      721 gatgtacaag ctgcccaaca ccatcagcag cgagaccttc ctgaagggcg gcggcggatc
      781 cggtgagggc agaggaagtc ttctaacatg cggtgacgtg gaggagaatc cgggccccat
      841 ggctcgggac cctctgccat tctttccacc tctgtacc
//
