LOCUS       sequence-377927-        3957 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GFP containing reporter plasmid expressing it under the control of 
            the OxyR/H2O2 inducible pAhpC promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3957)
  AUTHORS   Soudier P, Zuniga A, Duigou T, Voyvodic PL, Bazi-Kabbaj K, Kushwaha 
            M, Vendrell JA, Solassol J, Bonnet J, Faulon JL
  TITLE     PeroxiHUB: A Modular Cell-Free Biosensing Platform Using H(2)O(2) as
            Signal Integrator.
  JOURNAL   ACS Synth Biol. 2022 Aug 19;11(8):2578-2588. doi: 
            10.1021/acssynbio.2c00138. Epub 2022 Aug 1.
  PUBMED    35913043
REFERENCE   2  (bases 1 to 3957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.2c00138"; journalName: "ACS Synth Biol"; date: 
            "2022-08-19- 19"; volume: "11"; issue: "8"; pages: "2578-2588"
COMMENT     Sequence Label: pBeast-pAhpC-RBS- sfGFP
FEATURES             Location/Qualifiers
     source          1..3957
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     616..635
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(854..958)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1026..1044
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      1396..1423
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     RBS             1840..1851
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             1858..2571
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      2679..2750
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2766..2793
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(3020..3037)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3191..3779)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3271..3290)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3950..3957,1..784,785..853))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
       61 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
      121 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
      181 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
      241 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
      301 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
      361 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
      421 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
      481 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
      541 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
      601 cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
      661 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
      721 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
      781 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
      841 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
      901 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
      961 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     1021 ataaaaatag gcgtatcacg aggccctttc gtcttcaaga attctggcga atcctctgac
     1081 cagccagaaa acgacctttc tgtggtgaaa ccggatgctg caattcagag cgccagcaag
     1141 tgggggacag cagaagacct gaccgccgca gagtggatgt ttgacatggt gaagactatc
     1201 gcaccatcag ccagaaaacc gaattttgct gggtgggcta acgatatccg cctgatgcgt
     1261 gaacgtgacg gacgtaacca ccgcgacatg tgtgtgctgt tccgctggct cggataccct
     1321 tactctgttg aaaacgaata gataggttaa ggaacggtta tttctgcgta gatctatctt
     1381 acacagcatc acactggctc accttcgggt gggcctttct gcgtttatat actagagaga
     1441 gaatataaaa agccagatta ttaatccggc ttttttatta tttaggcaac tgaaacgatt
     1501 cggatcctgt attactattc ttaggagctt agatcaggtg attgcccttt gtttatgagg
     1561 gtgttgtaat ccatgtcgtt gttgcatttg taagggcaac acctcagcct gcaggcaggc
     1621 actgaagata ccaaagggta gttcagatta cacggtcacc tggaaagggg gccattttac
     1681 tttttatcgc cgctggcggt gcaaagttca caaagttgtc ttacgaaggt tgtaaggtaa
     1741 aacttatcga tttgataatg gaaacgcatt agccgaatcg gcaaaaattg gttaccttac
     1801 atctcatcga aaacacggag gaagtataga tgtctagaga aagaggagaa atactagatg
     1861 cgtaaaggcg aagagctgtt cactggtgtc gtccctattc tggtggaact ggatggtgat
     1921 gtcaacggtc ataagttttc cgtgcgtggc gagggtgaag gtgacgcaac taatggtaaa
     1981 ctgacgctga agttcatctg tactactggt aaactgccgg taccttggcc gactctggta
     2041 acgacgctga cttatggtgt tcagtgcttt gctcgttatc cggaccatat gaagcagcat
     2101 gacttcttca agtccgccat gccggaaggc tatgtgcagg aacgcacgat ttcctttaag
     2161 gatgacggca cgtacaaaac gcgtgcggaa gtgaaatttg aaggcgatac cctggtaaac
     2221 cgcattgagc tgaaaggcat tgactttaaa gaagacggca atatcctggg ccataagctg
     2281 gaatacaatt ttaacagcca caatgtttac atcaccgccg ataaacaaaa aaatggcatt
     2341 aaagcgaatt ttaaaattcg ccacaacgtg gaggatggca gcgtgcagct ggctgatcac
     2401 taccagcaaa acactccaat cggtgatggt cctgttctgc tgccagacaa tcactatctg
     2461 agcacgcaaa gcgttctgtc taaagatccg aacgagaaac gcgatcatat ggttctgctg
     2521 gagttcgtaa ccgcagcggg catcacgcat ggtatggatg aactgtacaa atgatgataa
     2581 gcttacttta tctgagaata gtcaatcttc ggaaatccca ggtggcatgc taaaagtctc
     2641 gtaaagcgtt ctatcaataa cccgttggtg ccaggcatca aataaaacga aaggctcagt
     2701 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
     2761 acactggctc accttcgggt gggcctttct gcgtttatac cgtctcagaa tcggccgtga
     2821 acaataaaat agtttcggta ttattgacca cttccgagta gaatcgtgct tcagtaagag
     2881 tcgaccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat
     2941 gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc
     3001 ggcagcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     3061 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     3121 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     3181 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     3241 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     3301 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     3361 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     3421 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     3481 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     3541 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     3601 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     3661 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     3721 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     3781 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     3841 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     3901 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaat
//
