LOCUS       sequence-377931-        3726 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GFP containing reporter plasmid expressing it under the control of 
            the reportedely OxyR/H2O2 inducible pYjjZ promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3726)
  AUTHORS   Soudier P, Zuniga A, Duigou T, Voyvodic PL, Bazi-Kabbaj K, Kushwaha 
            M, Vendrell JA, Solassol J, Bonnet J, Faulon JL
  TITLE     PeroxiHUB: A Modular Cell-Free Biosensing Platform Using H(2)O(2) as
            Signal Integrator.
  JOURNAL   ACS Synth Biol. 2022 Aug 19;11(8):2578-2588. doi: 
            10.1021/acssynbio.2c00138. Epub 2022 Aug 1.
  PUBMED    35913043
REFERENCE   2  (bases 1 to 3726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.2c00138"; journalName: "ACS Synth Biol"; date: 
            "2022-08-19- 19"; volume: "11"; issue: "8"; pages: "2578-2588"
COMMENT     Sequence Label: pBeast-pYjjZ-RBS- sfGFP
FEATURES             Location/Qualifiers
     source          1..3726
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             108..821
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      929..1000
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1016..1043
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(1270..1287)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1441..2029)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1521..1540)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2200..2991,2992..3060))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2823..2842
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3061..3165)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3233..3251
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      3603..3630
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 gaatcatagc gataggttta cccgatagca agggatttat ctggcttgca aatgataaaa
       61 attatcatat gatattggtt atcattatca atgaaagaga tgaaatcatg cgtaaaggcg
      121 aagagctgtt cactggtgtc gtccctattc tggtggaact ggatggtgat gtcaacggtc
      181 ataagttttc cgtgcgtggc gagggtgaag gtgacgcaac taatggtaaa ctgacgctga
      241 agttcatctg tactactggt aaactgccgg taccttggcc gactctggta acgacgctga
      301 cttatggtgt tcagtgcttt gctcgttatc cggaccatat gaagcagcat gacttcttca
      361 agtccgccat gccggaaggc tatgtgcagg aacgcacgat ttcctttaag gatgacggca
      421 cgtacaaaac gcgtgcggaa gtgaaatttg aaggcgatac cctggtaaac cgcattgagc
      481 tgaaaggcat tgactttaaa gaagacggca atatcctggg ccataagctg gaatacaatt
      541 ttaacagcca caatgtttac atcaccgccg ataaacaaaa aaatggcatt aaagcgaatt
      601 ttaaaattcg ccacaacgtg gaggatggca gcgtgcagct ggctgatcac taccagcaaa
      661 acactccaat cggtgatggt cctgttctgc tgccagacaa tcactatctg agcacgcaaa
      721 gcgttctgtc taaagatccg aacgagaaac gcgatcatat ggttctgctg gagttcgtaa
      781 ccgcagcggg catcacgcat ggtatggatg aactgtacaa atgatgataa gcttacttta
      841 tctgagaata gtcaatcttc ggaaatccca ggtggcatgc taaaagtctc gtaaagcgtt
      901 ctatcaataa cccgttggtg ccaggcatca aataaaacga aaggctcagt cgaaagactg
      961 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc
     1021 accttcgggt gggcctttct gcgtttatac cgtctcagaa tcggccgtga acaataaaat
     1081 agtttcggta ttattgacca cttccgagta gaatcgtgct tcagtaagag tcgaccgatg
     1141 cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc
     1201 gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc
     1261 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     1321 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     1381 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     1441 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     1501 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     1561 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     1621 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     1681 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     1741 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     1801 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     1861 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     1921 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     1981 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     2041 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     2101 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     2161 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     2221 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     2281 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     2341 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     2401 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     2461 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     2521 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     2581 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     2641 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     2701 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     2761 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg
     2821 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     2881 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     2941 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     3001 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     3061 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     3121 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     3181 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     3241 tatcacgagg ccctttcgtc ttcaagaatt ctggcgaatc ctctgaccag ccagaaaacg
     3301 acctttctgt ggtgaaaccg gatgctgcaa ttcagagcgc cagcaagtgg gggacagcag
     3361 aagacctgac cgccgcagag tggatgtttg acatggtgaa gactatcgca ccatcagcca
     3421 gaaaaccgaa ttttgctggg tgggctaacg atatccgcct gatgcgtgaa cgtgacggac
     3481 gtaaccaccg cgacatgtgt gtgctgttcc gctggctcgg atacccttac tctgttgaaa
     3541 acgaatagat aggttaagga acggttattt ctgcgtagat ctatcttaca cagcatcaca
     3601 ctggctcacc ttcgggtggg cctttctgcg tttatatact agagagagaa tataaaaagc
     3661 cagattatta atccggcttt tttattattt aggcaactga aacgattcgg atcctgtatt
     3721 actatt
//
