LOCUS       sequence-377941-        4524 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Compact T-DNA binary vector of the pLX series suitable for assembly 
            of plant virus infectious clones and their Agrobacterium-mediated 
            delivery to plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4524)
  AUTHORS   Pasin F
  TITLE     Assembly of plant virus agroinfectious clones using biological 
            material or DNA synthesis.
  JOURNAL   STAR Protoc. 2022 Dec 16;3(4):101716. doi: 
            10.1016/j.xpro.2022.101716. Epub 2022 Sep 22.
  PUBMED    36149792
REFERENCE   2  (bases 1 to 4524)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "STAR 
            Protoc."; date: "2022-12-16"; volume: "3(4):101716. doi"; pages: " 
            10.1016/j.xpro.2022.101716. Epub 2022 Sep 22"
COMMENT     Sequence Label: pLX-AS
FEATURES             Location/Qualifiers
     source          1..4524
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    133..157
                     /label=LB T-DNA repeat
                     /note="left border repeat from octopine Ach5 T-DNA"
     misc_feature    184..208
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     1149..1169
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     promoter        1158..1204
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     promoter        complement(join(1485..1491,1492..1509,1510..1515))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     terminator      1573..1825
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(2062..2877)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2869..2888
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             complement(3003..3665)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      3666..4437
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
ORIGIN
        1 ccatcatcag ttcggtggtc ttccgacgaa caataaggcc gcaaatcgcg gcctttttta
       61 ttgataacaa aaccggctca gttctgcgta gaaaccaaca tgcaagctcc accgggtgca
      121 aagcggcagc ggcggcagga tatattcaat tgtaaatggc ttcatgtccg ggaaatctac
      181 tggtggcagg atatattgtg gtgtaaacaa tggagaaaaa gattaattaa gtactagtag
      241 cctggtcgaa accgtctcac cagtacgcac gattcaaggc ttgcttcaca aaccaaggca
      301 agtaatagag attggagtct ctaaaaaggt agttcccact gaatcaaagg ccatggagtc
      361 aaagattcaa atagaggacc taacagaact cgccgtaaag actggcgaac agttcataca
      421 gagtctctta cgactcaatg acaagaagaa aatcttcgtc aacatggtgg agcacgacac
      481 acttgtctac tccaaaaata tcaaagatac agtctcagaa gaccaaaggg caattgagac
      541 ttttcaacaa agggtaatat ccggaaacct cctcggattc cattgcccag ctatctgtca
      601 ctttattgtg aagatagtgg aaaaggaagg tggctcctac aaatgccatc attgcgataa
      661 aggaaaggcc atcgttgaag atgcctctgc cgacagtggt cccaaagatg gacccccacc
      721 cacgaggagc atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg
      781 atgtgataac atggtggagc acgacacact tgtctactcc aaaaatatca aagatacagt
      841 ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg gaaacctcct
      901 cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa aggaaggtgg
      961 ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatc ctctgccgac
     1021 agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca
     1081 accacgtctt caaagcaagt ggattgatgt gatatctcca ctgacgtaag ggatgacgca
     1141 caatcccact atccttcgca agacccttcc tctatataag gaagttcatt tcatttggag
     1201 aggaagagca ggtgtcagga gagggaccaa aagcaaaaac ccgccgaagc gggttactag
     1261 cgccattcgc cattcagaga gcggagctgc tgcgacggac gatcggtacg cgcctcttcg
     1321 ctattacgcc aactggcgaa aggtggatgt gctgcaaggc gattaagttg ggtaacgcca
     1381 gggttttccc agtcacgacg ttgtagtacc acggcaaggc tatctgtaat cattgttgta
     1441 ctccggttag gacggattgg gaactggcta actcaaaatc cacacattat acgagccgga
     1501 agcataaagt gtaaagcctg gggtgcctaa tgagtgagtt ctctcgctgt cacctgctct
     1561 tcccagcctg cggatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt
     1621 gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt
     1681 aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta
     1741 tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc
     1801 gcggtgtcat ctatgttact cgatctcgtc aggagacggg acaaggatgc gagctagcct
     1861 gcaggaattg ttgattttgt gatgactgat ggcaggatat atgcggttgt aattcatttt
     1921 tattgtctaa atttctgtat ttgtttgttt gttcggttgt aaattttttt ggaagaacaa
     1981 gaaaagaaaa aacacccgtt agggtgtttt tagttagtgt ggcgcgccga cttgcgacat
     2041 gcggtccttt gcaatcaact attagaaaaa ttcatccagc atcagatgaa attgcagttt
     2101 gttcatatcc ggattatcaa tgccatattt ctgaaacaga cgtttttgca ggctcgggct
     2161 aaattcgccc aggcagttcc acagaatggc cagatcctga taacgatccg caatgcccac
     2221 acggcccaca tcaatgcagc caatcagttt gccttcatcg aaaatcaggt tatccaggct
     2281 aaaatcgccg tgggtcacca cgctatccgg gctaaacggc agcagtttat gcatttcttt
     2341 ccacacctgt tccaccggcc agccgttacg ttcatcatca aaatcgctcg catccaccag
     2401 gccgttgttc atacggctct gcgcctgggc cagacgaaac acacgatcgc tgttaaacgg
     2461 gcagttgcac accggaatgc tatgcagacg acgcagaaac acggccagcg catccacaat
     2521 gttttcgccg ctatccggat attcttccag cacctgaaac gcggttttgc ccggaatcgc
     2581 ggtggtcagc agccacgcat catccggggt gcgaataaaa tgtttaatgg tcggcagcgg
     2641 cataaattcg gtcagccagt tcagacgcac catttcatcg gtcacatcgt tcgccacgct
     2701 gcctttgcca tgtttcagaa acagttccgg cgcatccggt ttgccataca gacgataaat
     2761 ggtcgcgccg ctctgaccca cgttatcacg cgcccattta tagccataca gatccgcatc
     2821 catgttgctg ttcagacgcg gacggctaca gctcgtttca cgctgaatat ggctcataac
     2881 accccttgta ttactgttta tgtaagcaga cagttttatt gttcatgatg atatattttt
     2941 atcttgtgca atgtaacatc agagattttg agacacaaga tcggattggc ggttatgcgg
     3001 ttctaccggc gcggcagcgt tacccgtgtc ggcggctcca acggctcgcc atcgtccaga
     3061 aaacacggct catcgggcat cggcaggcgc tgctgcccgc gccgttccca ttcctccgtt
     3121 tcggtcaagg ctggcaggtc tggttccatg cccggaatgc cgggctggct gggcggctcc
     3181 tcgccggggc cggtcggtag ttgctgctcg cccggataca gggtcgggat gcggcgcagg
     3241 tcgccatgcc ccaacagcga ttcgtcctgg tcgtcgtgat caaccaccac ggcggcactg
     3301 aacaccgaca ggcgcaactg gtcgcggggc tggccccacg ccacgcggtc attgaccacg
     3361 taggccgaca cggtgccggg gccgttgagc ttcacgacgg agatccagcg ctcggccacc
     3421 aagtccttga ctgcgtattg gaccgtccgc aaagaacgtc cgatgagctt ggaaagtgtc
     3481 ttctggctga ccaccacggc gttctggtgg cccatctgcg ccacgaggtg atgcagcagc
     3541 attgccgccg tgggtttcct cgcaataagc ccggcccacg cctcatgcgc tttgcgttcc
     3601 gtttgcaccc agtgaccggg cttgttcttg gcttgaatgc cgatttctct ggactgcgtg
     3661 gccatgctta tctccatgcg gtaggggtgc cgcacggttg cggcaccatg cgcaatcagc
     3721 tgcaactttt cggcagcgcg acaacaatta tgcgttgcgt aaaagtggca gtcaattaca
     3781 gattttcttt aacctacgca atgagctatt gcggggggtg ccgcaatgag ctgttgcgta
     3841 cccccctttt ttaagttgtt gatttttaag tctttcgcat ttcgccctat atctagttct
     3901 ttggtgccca aagaagggca cccctgcggg gttcccccac gccttcggcg cggctccccc
     3961 tccggcaaaa agtggcccct ccggggcttg ttgatcgact gcgcggcctt cggccttgcc
     4021 caaggtggcg ctgccccctt ggaacccccg cactcgccgc cgtgaggctc ggggggcagg
     4081 cgggcgggct tcgcccttcg actgccccca ctcgcatagg cttgggtcgt tccaggcgcg
     4141 tcaaggccaa gccgctgcgc ggtcgctgcg cgagccttga cccgccttcc acttggtgtc
     4201 caaccggcaa gcgaagcgcg caggccgcag gccggaggct tttccccaga gaaaattaaa
     4261 aaaattgatg gggcaaggcc gcaggccgcg cagttggagc cggtgggtat gtggtcgaag
     4321 gctgggtagc cggtgggcaa tccctgtggt caagctcgtg ggcaggcgca gcctgtccat
     4381 cagcttgtcc agcagggttg tccacgggcc gagcgaagcg agccagccgg tggccgctcg
     4441 cggccatcgt ccacatatcc acgggctggc aagggagcgc agcgaccgcg cagggcgaag
     4501 cccggagagc aagcccgtag gggg
//
