LOCUS       sequence-377959-       11718 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To test if the TCTP promoter can drive the Flp recomhinase in order 
            to remove the OCS terminator and increase circuit output..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11718)
  AUTHORS   Lloyd JPB, Ly F, Gong P, Pflueger J, Swain T, Pflueger C, Fourie E, 
            Khan MA, Kidd BN, Lister R
  TITLE     Synthetic memory circuits for stable cell reprogramming in plants.
  JOURNAL   Nat Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2.
  PUBMED    35788565
REFERENCE   2  (bases 1 to 11718)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2."
COMMENT     Sequence Label: LLP735_L2pV1_1I_OCS-rLUC_F-TCTP-FlpO
FEATURES             Location/Qualifiers
     source          1..11718
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    845..878
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     terminator      879..1586
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     protein_bind    1587..1620
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     intron          1687..1875
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     polyA_signal    2903..3077
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             3465..5117
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(3531..3549)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      5137..5337
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     misc_feature    5680..5735
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             5743..5763
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          6447..6635
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     polyA_signal    7259..7433
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    7496..7520
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      7608..8318
                     /label=oriV
                     /note="incP origin of replication"
     promoter        8339..8443
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8444..9238
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      9409..9997
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9898..9917
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             10333..11481
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    11627..11651
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgaatt cggatccgga gtcgacaaaa tttagaacga acttaattat gatctcaaat
       61 acattgatac atatctcatc tagatctagg ttatcattat gtaagaaagt tttgacgaat
      121 atggcacgac aaaatggcta gactcgatgt aattggtatc tcaactcaac attatactta
      181 taccaaacat tagttagaca aaatttaaac aactattttt tatgtatgca agagtcagca
      241 tatgtataat tgattcagaa tcgttttgac gagttcggat gtagtagtag ccattattta
      301 atgtacatac taatcgtgaa tagtgaatat gatgaaacat tgtatcttat tgtataaata
      361 tccataaaca catcatgaaa gacactttct ttcacggtct gaattaatta tgatacaatt
      421 ctaatagaaa acgaattaaa ttacgttgaa ttgtatgaaa tctaattgaa caagccaacc
      481 acgacgacga ctaacgttgc ctggattgac tcggtttaag ttaaccacta aaaaaacgga
      541 gctgtcatgt aacacgcgga tcgagcaggt cacagtcatg aagccatcaa agcaaaagaa
      601 ctaatccaag ggctgagatg attaattagt ttaaaaatta gttaacacga gggaaaaggc
      661 tgtctgacag ccaggtcacg ttatctttac ctgtggtcga aatgattcgt gtctgtcgat
      721 tttaattatt tttttgaaag gccgaaaata aagttgtaag agataaaccc gcctatataa
      781 attcatatat tttcctctcc gctttgaata ctgtattttt acaacaatta ccaacaacaa
      841 caaagaagtt cctattctct agaaagtata ggaacttcct gctttaatga gatatgcgag
      901 aagcctatga tcgcatgata tttgctttca attctgttgt gcacgttgta aaaaacctga
      961 gcatgtgtag ctcagatcct taccgccggt ttcggttcat tctaatgaat atatcacccg
     1021 ttactatcgt atttttatga ataatattct ccgttcaatt tactgattgt accctactac
     1081 ttatatgtac aatattaaaa tgaaaacaat atattgtgct gaataggttt atagcgacat
     1141 ctatgataga gcgccacaat aacaaacaat tgcgttttat tattacaaat ccaattttaa
     1201 aaaaagcggc agaaccggtc aaacctaaaa gactgattac ataaatctta ttcaaatttc
     1261 aaaagtgccc caggggctag tatctacgac acaccgagcg gcgaactaat aacgctcact
     1321 gaagggaact ccggttcccc gccggcgcgc atgggtgaga ttccttgaag ttgagtattg
     1381 gccgtccgct ctaccgaaag ttacgggcac cattcaaccc ggtccagcac ggcggccggg
     1441 taaccgactt gctgccccga gaattatgca gcattttttt ggtgtatgtg ggccccaaat
     1501 gaagtgcagg tcaaaccttg acagtgacga caaatcgttg ggcgggtcca gggcgaattt
     1561 tgcgacaaca tgtcgaggct cagcaggaag ttcctattct ctagaaagta taggaacttc
     1621 caacaaacaa cattacaatt actatttaca attacaatga cttcaaaggt gtatgatcct
     1681 gagcaggtaa gtttctgctt ctacctttga tatatatata ataattatca ttaattagta
     1741 gtaatataat atttcaaata tttttttcaa aataaaagaa tgtagtatat agcaattgct
     1801 tttctgtagt ttataagtgt gtatatttta atttataact tttctaatat atgaccaaaa
     1861 tttgttgatg tgcagagaaa aagaatgata actggtccac aatggtgggc aagatgtaaa
     1921 cagatgaacg tgcttgattc tttcattaat tactacgatt cagaaaagca cgctgagaat
     1981 gcagttatct ttcttcacgg aaacgctgca tcttcatatt tgtggagaca tgttgtgcct
     2041 cacattgaac cagtggctag gtgtattatc ccagatctta tcggaatggg aaagtctggt
     2101 aaatctggaa atggttctta cagacttttg gatcattata agtacctcac agcttggttc
     2161 gaacttctca acttacctaa gaagattatt ttcgttggac acgattgggg tgcttgcttg
     2221 gcattccatt actcatacga acaccaagat aagatcaaag ctatagttca tgcagagagt
     2281 gttgtggatg tgatagaatc ttgggatgag tggccagata ttgaagagga tattgctttg
     2341 atcaagtctg aagagggaga aaagatggtt ctcgaaaata actttttcgt ggaaactatg
     2401 cttccttcaa agattatgag aaaattggaa ccagaagagt ttgctgcata tctcgaacct
     2461 ttcaaggaaa aaggagaggt tagaaggcca acactcagtt ggcctaggga aataccatta
     2521 gttaagggag gtaaacctga tgttgtgcag atcgtgagaa attataacgc ttaccttagg
     2581 gcatcagatg atttgcctaa gatgttcatc gagtcagatc caggattttt cagtaatgct
     2641 atagttgaag gtgcaaagaa atttccaaac accgagttcg ttaaggtgaa aggattgcat
     2701 ttctcacaag aagatgctcc tgatgagatg ggaaagtata ttaagtcttt tgttgaaaga
     2761 gtgctcaaaa acgagcaggg ttctcgatct gatgcttctg aagtaacgtc aacaagctcc
     2821 cagtctgagg tatgcacagt ggaaacgcca ggttgtgttg gttcttaagc ttctctagct
     2881 agagtcgatc gacaagctcg agtttctcca taataatgtg tgagtagttc ccagataagg
     2941 gaattagggt tcctataggg tttcgctcat gtgttgagca tataagaaac ccttagtatg
     3001 tatttgtatt tgtaaaatac ttctatcaat aaaatttcta attcctaaaa ccaaaatcca
     3061 gtactaaaat ccagatcgct gcaagaattc aagcttggag ccaacactcg aatccccacc
     3121 cgttggacca aacccggctc attaagcgtc ggttcagatt tatttccttt atttaaaaaa
     3181 aaggaaaggg taaaaaatag aaaattggaa acagttaaag cccaaaattg taatttaccg
     3241 agaattgtaa atttacctga aaaccctacg ctatagtttc gactataaat accaaactta
     3301 ggacctcact tcagaatccc ctcgtcgctg cgtctctctc ccgcaacctt cgattttcgt
     3361 ttattcgcat ccatcggaga gagaaaacaa tcaataagcg acctactaca aacaagtaag
     3421 taagagaaaa accaaaagaa gaagagaaac aacaagaaga agtaatggag gacgccaaaa
     3481 acataaagaa aggcccggcg ccattctatc ctctagagga tggaaccgct ggagagcaac
     3541 tgcataaggc tatgaagaga tacgccctgg ttcctggaac aattgctttt acagatgcac
     3601 atatcgaggt gaacatcacg tacgcggaat acttcgaaat gtccgttcgg ttggcagaag
     3661 ctatgaaacg atatgggctg aatacaaatc acagaatcgt cgtatgcagt gaaaactctc
     3721 ttcaattctt tatgccggtg ttgggcgcgt tatttatcgg agttgcagtt gcgcccgcga
     3781 acgacattta taatgaacgt gaattgctca acagtatgaa catttcgcag cctaccgtag
     3841 tgtttgtttc caaaaagggg ttgcaaaaaa ttttgaacgt gcaaaaaaaa ttaccaataa
     3901 tccagaaaat tattatcatg gattctaaaa cggattacca gggatttcag tcgatgtaca
     3961 cgttcgtcac atctcatcta cctcccggtt ttaatgaata cgattttgta ccagagtcct
     4021 ttgatcgtga caaaacaatt gcactgataa tgaattcctc tggatctact gggttaccta
     4081 agggtgtggc ccttccgcat agaactgcct gcgtcagatt ctcgcatgcc agagatccta
     4141 tttttggcaa tcaaatcatt ccggatactg cgattttaag tgttgttcca ttccatcacg
     4201 gttttggaat gtttactaca ctcggatatt tgatatgtgg atttcgagtc gtcttaatgt
     4261 atagatttga agaagagctg tttttacgat cccttcagga ttacaaaatt caaagtgcgt
     4321 tgctagtacc aaccctattt tcattcttcg ccaaaagcac tctgattgac aaatacgatt
     4381 tatctaattt acacgaaatt gcttctgggg gcgcacctct ttcgaaagaa gtcggggaag
     4441 cggttgcaaa acgcttccat cttccaggga tacgacaagg atatgggctc actgagacta
     4501 catcagctat tctgattaca cccgaggggg atgataaacc gggcgcggtc ggtaaagttg
     4561 ttccattttt tgaagcgaag gttgtggatc tggataccgg gaaaacgctg ggcgttaatc
     4621 agagaggcga attatgtgtc agaggaccta tgattatgtc cggttatgta aacaatccgg
     4681 aagcgaccaa cgccttgatt gacaaggatg gatggctaca ttctggagac atagcttact
     4741 gggacgagga cgaacacttc ttcatagttg accgcttgaa gtctttaatt aaatacaaag
     4801 gatatcaggt ggcccccgct gaattggaat cgatattgtt acaacacccc aacatcttcg
     4861 acgcgggcgt ggcaggtctg cccgacgatg acgccggtga acttcccgcc gccgttgttg
     4921 ttttggagca cggaaagacg atgacggaaa aagagatcgt ggattacgtc gccagtcaag
     4981 taacaaccgc gaaaaagttg cgcggaggag ttgtgtttgt ggacgaagta ccgaaaggtc
     5041 ttaccggaaa actcgacgca agaaaaatca gagagatcct cataaaggcc aagaagggcg
     5101 gaaagtccaa attgtaagct tgatcccccg tcgacagcta gctatatcat caatttatgt
     5161 attacacata atatcgcact cagtctttca tctacggcaa tgtaccagct gatataatca
     5221 gttattgaaa tatttctgaa tttaaacttg catcaataaa tttatgtttt tgcttggact
     5281 ataatacctg acttgttatt ttatcaataa atatttaaac tatatttctt tcaagatcgc
     5341 tactagaatt cgagctcgga gccaacactc gaatccccac ccgttggacc aaacccggct
     5401 cattaagcgt cggttcagat ttatttcctt tatttaaaaa aaaggaaagg gtaaaaaata
     5461 gaaaattgga aacagttaaa gcccaaaatt gtaatttacc gagaattgta aatttacctg
     5521 aaaaccctac gctatagttt cgactataaa taccaaactt aggacctcac ttcagaatcc
     5581 cctcgtcgct gcgtctctct cccgcaacct tcgattttcg tttattcgca tccatcggag
     5641 agagaaaaca atcaataagc gacctactgt atttttacaa caattaccaa caacaacaaa
     5701 caacaaacaa cattacaatt actatttaca attacaatgg ctcctaagaa gaagaggaag
     5761 gtgatgagcc agttcgacat cctgtgcaag acccccccca aggtgctggt gcggcagttc
     5821 gtggagagat tcgagaggcc cagcggcgag aagatcgcca gctgtgccgc cgagctgacc
     5881 tacctgtgct ggatgatcac ccacaacggc accgccatca agagggccac cttcatgagc
     5941 tacaacacca tcatcagcaa cagcctgagc ttcgacatcg tgaacaagag cctgcagttc
     6001 aagtacaaga cccagaaggc caccatcctg gaggccagcc tgaagaagct gatccccgcc
     6061 tgggagttca ccatcatccc ttacaacggc cagaagcacc agagcgacat caccgacatc
     6121 gtgtccagcc tgcagctgca gttcgagagc agcgaggagg ccgacaaggg caacagccac
     6181 agcaagaaga tgctgaaggc cctgctgtcc gagggcgaga gcatctggga gatcaccgag
     6241 aagatcctga acagcttcga gtacaccagc aggttcacca agaccaagac cctgtaccag
     6301 ttcctgttcc tggccacatt catcaactgc ggcaggttca gcgacatcaa gaacgtggac
     6361 cccaagagct tcaagctggt gcagaacaag tacctgggcg tgatcattca gtgcctggtg
     6421 accgagacta agacaagcgt gtccaggtaa gtttctgctt ctacctttga tatatatata
     6481 ataattatca ttaattagta gtaatataat atttcaaata tttttttcaa aataaaagaa
     6541 tgtagtatat agcaattgct tttctgtagt ttataagtgt gtatatttta atttataact
     6601 tttctaatat atgaccaaaa tttgttgatg tgcaggcaca tctacttttt cagcgccaga
     6661 ggcaggatcg accccctggt gtacctggac gagttcctga ggaacagcga gcccgtgctg
     6721 aagagagtga acaggaccgg caacagcagc agcaacaagc aggagtacca gctgctgaag
     6781 gacaacctgg tgcgcagcta caacaaggcc ctgaagaaga acgcccccta ccccatcttc
     6841 gctatcaaga acggccctaa gagccacatc ggcaggcacc tgatgaccag ctttctgagc
     6901 atgaagggcc tgaccgagct gacaaacgtg gtgggcaact ggagcgacaa gagggcctcc
     6961 gccgtggcca ggaccaccta cacccaccag atcaccgcca tccccgacca ctacttcgcc
     7021 ctggtgtcca ggtactacgc ctacgacccc atcagcaagg agatgatcgc cctgaaggac
     7081 gagactaacc ccatcgagga gtggcagcac atcgagcagc tgaagggcag cgccgagggc
     7141 agcatcagat accccgcctg gaacggcatc atcagccagg aggtgctgga ctacctgagc
     7201 agctacatca acaggcggat ctgagcttct ctagctagag tcgatcgaca agctcgagtt
     7261 tctccataat aatgtgtgag tagttcccag ataagggaat tagggttcct atagggtttc
     7321 gctcatgtgt tgagcatata agaaaccctt agtatgtatt tgtatttgta aaatacttct
     7381 atcaataaaa tttctaattc ctaaaaccaa aatccagtac taaaatccag atcgctttac
     7441 gaggatgcac atgtgaccga gggacacgaa gtgatccgtt taaactatca gtgtttgaca
     7501 ggatatattg gcgggtaaac ctaagagaaa agagcgttta ttagaataat cggatattta
     7561 aaagggcgtg aaaaggttta tccgttcgtc catttgtatg tgccagccgc ctttgcgacg
     7621 ctcaccgggc tggttgccct cgccgctggg ctggcggccg tctatggccc tgcaaacgcg
     7681 ccagaaacgc cgtcgaagcc gtgtgcgaga caccgcggcc gccggcgttg tggatacctc
     7741 gcggaaaact tggccctcac tgacagatga ggggcggacg ttgacacttg aggggccgac
     7801 tcacccggcg cggcgttgac agatgagggg caggctcgat ttcggccggc gacgtggagc
     7861 tggccagcct cgcaaatcgg cgaaaacgcc tgattttacg cgagtttccc acagatgatg
     7921 tggacaagcc tggggataag tgccctgcgg tattgacact tgaggggcgc gactactgac
     7981 agatgagggg cgcgatcctt gacacttgag gggcagagtg ctgacagatg aggggcgcac
     8041 ctattgacat ttgaggggct gtccacaggc agaaaatcca gcatttgcaa gggtttccgc
     8101 ccgtttttcg gccaccgcta acctgtcttt taacctgctt ttaaaccaat atttataaac
     8161 cttgttttta accagggctg cgccctgtgc gcgtgaccgc gcacgccgaa ggggggtgcc
     8221 cccccttctc gaaccctccc ggcccgctaa cgcgggcctc ccatcccccc aggggctgcg
     8281 cccctcggcc gcgaacggcc tcaccccaaa aatggcagcg ctggccaatt cccgagtgcg
     8341 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     8401 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatggcta aaatgagaat
     8461 atcaccggaa ttgaaaaaac tgatcgaaaa ataccgctgc gtaaaagata cggaaggaat
     8521 gtctcctgct aaggtatata agctggtggg agaaaatgaa aacctatatt taaaaatgac
     8581 ggacagccgg tataaaggga ccacctatga tgtggaacgg gaaaaggaca tgatgctatg
     8641 gctggaagga aagctgcctg ttccaaaggt cctgcacttt gaacggcatg atggctggag
     8701 caatctgctc atgagtgagg ccgatggcgt cctttgctcg gaagagtatg aagatgaaca
     8761 aagccctgaa aagattatcg agctgtatgc ggagtgcatc aggctctttc actccatcga
     8821 catatcggat tgtccctata cgaatagctt agacagccgc ttagccgaat tggattactt
     8881 actgaataac gatctggccg atgtggattg cgaaaactgg gaagaggaca ctccatttaa
     8941 agatccgcgc gagctgtatg attttttaaa gacggaaaag cccgaagagg aacttgtctt
     9001 ttcccacggc gacctgggag acagcaacat ctttgtgaaa gatggcaaag taagtggctt
     9061 tattgatctt gggagaagcg gcagggcgga caagtggtat gacattgcct tctgcgtccg
     9121 gtcgctcagg gaggatatcg gggaagaaca gtatgtcgag ctattttttg acttactggg
     9181 gatcaagcct gattgggaga aaataaaata ttatatttta ctggatgaat tgttttagct
     9241 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     9301 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     9361 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     9421 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     9481 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     9541 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     9601 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     9661 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     9721 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     9781 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     9841 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     9901 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     9961 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
    10021 acggttcctg gcagatccta gatgtggcgc aacgatgccg gcgacaagca ggagcgcacc
    10081 gacttcttcc gcatcaagtg ttttggctct caggccgagg cccacggcaa gtatttgggc
    10141 aaggggtcgc tggtattcgt gcagggcaag attcggaata ccaagtacga gaaggacggc
    10201 cagacggtct acgggaccga cttcattgcc gataaggtgg attatctgga caccaaggca
    10261 ccaggcgggt caaatcagga ataagggcac attgccccgg cgtgagtcgg ggcaatcccg
    10321 caaggagggt gaatgaatcg gacgtttgac cggaaggcat acaggcaaga actgatcgac
    10381 gcggggtttt ccgccgagga tgccgaaacc atcgcaagcc gcaccgtcat gcgtgcgccc
    10441 cgcgaaacct tccagtccgt cggctcgatg gtccagcaag ctacggccaa gatcgagcgc
    10501 gacagcgtgc aactggctcc ccctgccctg cccgcgccat cggccgccgt ggagcgttcg
    10561 cgtcgtcttg aacaggaggc ggcaggtttg gcgaagtcga tgaccatcga cacgcgagga
    10621 actatgacga ccaagaagcg aaaaaccgcc ggcgaggacc tggcaaaaca ggtcagcgag
    10681 gccaagcagg ccgcgttgct gaaacacacg aagcagcaga tcaaggaaat gcagctttcc
    10741 ttgttcgata ttgcgccgtg gccggacacg atgcgagcga tgccaaacga cacggcccgc
    10801 tctgccctgt tcaccacgcg caacaagaaa atcccgcgcg aggcgctgca aaacaaggtc
    10861 attttccacg tcaacaagga cgtgaagatc acctacaccg gcgtcgagct gcgggccgac
    10921 gatgacgaac tggtgtggca gcaggtgttg gagtacgcga agcgcacccc tatcggcgag
    10981 ccgatcacct tcacgttcta cgagctttgc caggacctgg gctggtcgat caatggccgg
    11041 tattacacga aggccgagga atgcctgtcg cgcctacagg cgacggcgat gggcttcacg
    11101 tccgaccgcg ttgggcacct ggaatcggtg tcgctgctgc accgcttccg cgtcctggac
    11161 cgtggcaaga aaacgtcccg ttgccaggtc ctgatcgacg aggaaatcgt cgtgctgttt
    11221 gctggcgacc actacacgaa attcatatgg gagaagtacc gcaagctgtc gccgacggcc
    11281 cgacggatgt tcgactattt cagctcgcac cgggagccgt acccgctcaa gctggaaacc
    11341 ttccgcctca tgtgcggatc ggattccacc cgcgtgaaga agtggcgcga gcaggtcggc
    11401 gaagcctgcg aagagttgcg aggcagcggc ctggtggaac acgcctgggt caatgatgac
    11461 ctggtgcatt gcaaacgcta gggccttgtg gggtcagttc cggctggggg ttcagcagcc
    11521 cctgctcgga tctgttggac cggacagtag tcatggttga tgggctgcct gtatcgagtg
    11581 gtgattttgt gccgagctgc cggtcgggga gctgttggct ggctggtggc aggatatatt
    11641 gtggtgtaaa caaattgacg cttagacaac ttaataacac attgcggacg tttttaatgt
    11701 actggggttg aacactct
//
