LOCUS       sequence-377960-       12774 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To test if the Act2 promoter can drive the Flp recomhinase in order 
            to remove the OCS terminator and increase circuit output..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12774)
  AUTHORS   Lloyd JPB, Ly F, Gong P, Pflueger J, Swain T, Pflueger C, Fourie E, 
            Khan MA, Kidd BN, Lister R
  TITLE     Synthetic memory circuits for stable cell reprogramming in plants.
  JOURNAL   Nat Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2.
  PUBMED    35788565
REFERENCE   2  (bases 1 to 12774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2."
COMMENT     Sequence Label: LLP737_L2pV1_1I_Act2_FRT_OCS_Act2_Flp_lacZ
FEATURES             Location/Qualifiers
     source          1..12774
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    845..878
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     terminator      879..1586
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     protein_bind    1587..1620
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     intron          1687..1875
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     polyA_signal    2903..3077
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             3465..5117
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(3531..3549)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      5137..5337
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     misc_feature    6164..6219
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             6227..6247
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          6931..7119
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     polyA_signal    7743..7917
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     protein_bind    7987..8008
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8023..8028,8029..8046,8047..8053)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8061..8077
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8066..8088
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8085..8101
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8085..8101
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             8097..8420
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    8114..8170
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(8171..8188)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8171..8187)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8180..8202)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8396..8415)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    8552..8576
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      8664..9374
                     /label=oriV
                     /note="incP origin of replication"
     promoter        9395..9499
                     /gene="bla"
                     /label=AmpR promoter
     CDS             9500..10294
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      10465..11053
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10954..10973
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             11389..12537
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    12683..12707
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgaatt cggatccgga gtcgacaaaa tttagaacga acttaattat gatctcaaat
       61 acattgatac atatctcatc tagatctagg ttatcattat gtaagaaagt tttgacgaat
      121 atggcacgac aaaatggcta gactcgatgt aattggtatc tcaactcaac attatactta
      181 taccaaacat tagttagaca aaatttaaac aactattttt tatgtatgca agagtcagca
      241 tatgtataat tgattcagaa tcgttttgac gagttcggat gtagtagtag ccattattta
      301 atgtacatac taatcgtgaa tagtgaatat gatgaaacat tgtatcttat tgtataaata
      361 tccataaaca catcatgaaa gacactttct ttcacggtct gaattaatta tgatacaatt
      421 ctaatagaaa acgaattaaa ttacgttgaa ttgtatgaaa tctaattgaa caagccaacc
      481 acgacgacga ctaacgttgc ctggattgac tcggtttaag ttaaccacta aaaaaacgga
      541 gctgtcatgt aacacgcgga tcgagcaggt cacagtcatg aagccatcaa agcaaaagaa
      601 ctaatccaag ggctgagatg attaattagt ttaaaaatta gttaacacga gggaaaaggc
      661 tgtctgacag ccaggtcacg ttatctttac ctgtggtcga aatgattcgt gtctgtcgat
      721 tttaattatt tttttgaaag gccgaaaata aagttgtaag agataaaccc gcctatataa
      781 attcatatat tttcctctcc gctttgaata ctgtattttt acaacaatta ccaacaacaa
      841 caaagaagtt cctattctct agaaagtata ggaacttcct gctttaatga gatatgcgag
      901 aagcctatga tcgcatgata tttgctttca attctgttgt gcacgttgta aaaaacctga
      961 gcatgtgtag ctcagatcct taccgccggt ttcggttcat tctaatgaat atatcacccg
     1021 ttactatcgt atttttatga ataatattct ccgttcaatt tactgattgt accctactac
     1081 ttatatgtac aatattaaaa tgaaaacaat atattgtgct gaataggttt atagcgacat
     1141 ctatgataga gcgccacaat aacaaacaat tgcgttttat tattacaaat ccaattttaa
     1201 aaaaagcggc agaaccggtc aaacctaaaa gactgattac ataaatctta ttcaaatttc
     1261 aaaagtgccc caggggctag tatctacgac acaccgagcg gcgaactaat aacgctcact
     1321 gaagggaact ccggttcccc gccggcgcgc atgggtgaga ttccttgaag ttgagtattg
     1381 gccgtccgct ctaccgaaag ttacgggcac cattcaaccc ggtccagcac ggcggccggg
     1441 taaccgactt gctgccccga gaattatgca gcattttttt ggtgtatgtg ggccccaaat
     1501 gaagtgcagg tcaaaccttg acagtgacga caaatcgttg ggcgggtcca gggcgaattt
     1561 tgcgacaaca tgtcgaggct cagcaggaag ttcctattct ctagaaagta taggaacttc
     1621 caacaaacaa cattacaatt actatttaca attacaatga cttcaaaggt gtatgatcct
     1681 gagcaggtaa gtttctgctt ctacctttga tatatatata ataattatca ttaattagta
     1741 gtaatataat atttcaaata tttttttcaa aataaaagaa tgtagtatat agcaattgct
     1801 tttctgtagt ttataagtgt gtatatttta atttataact tttctaatat atgaccaaaa
     1861 tttgttgatg tgcagagaaa aagaatgata actggtccac aatggtgggc aagatgtaaa
     1921 cagatgaacg tgcttgattc tttcattaat tactacgatt cagaaaagca cgctgagaat
     1981 gcagttatct ttcttcacgg aaacgctgca tcttcatatt tgtggagaca tgttgtgcct
     2041 cacattgaac cagtggctag gtgtattatc ccagatctta tcggaatggg aaagtctggt
     2101 aaatctggaa atggttctta cagacttttg gatcattata agtacctcac agcttggttc
     2161 gaacttctca acttacctaa gaagattatt ttcgttggac acgattgggg tgcttgcttg
     2221 gcattccatt actcatacga acaccaagat aagatcaaag ctatagttca tgcagagagt
     2281 gttgtggatg tgatagaatc ttgggatgag tggccagata ttgaagagga tattgctttg
     2341 atcaagtctg aagagggaga aaagatggtt ctcgaaaata actttttcgt ggaaactatg
     2401 cttccttcaa agattatgag aaaattggaa ccagaagagt ttgctgcata tctcgaacct
     2461 ttcaaggaaa aaggagaggt tagaaggcca acactcagtt ggcctaggga aataccatta
     2521 gttaagggag gtaaacctga tgttgtgcag atcgtgagaa attataacgc ttaccttagg
     2581 gcatcagatg atttgcctaa gatgttcatc gagtcagatc caggattttt cagtaatgct
     2641 atagttgaag gtgcaaagaa atttccaaac accgagttcg ttaaggtgaa aggattgcat
     2701 ttctcacaag aagatgctcc tgatgagatg ggaaagtata ttaagtcttt tgttgaaaga
     2761 gtgctcaaaa acgagcaggg ttctcgatct gatgcttctg aagtaacgtc aacaagctcc
     2821 cagtctgagg tatgcacagt ggaaacgcca ggttgtgttg gttcttaagc ttctctagct
     2881 agagtcgatc gacaagctcg agtttctcca taataatgtg tgagtagttc ccagataagg
     2941 gaattagggt tcctataggg tttcgctcat gtgttgagca tataagaaac ccttagtatg
     3001 tatttgtatt tgtaaaatac ttctatcaat aaaatttcta attcctaaaa ccaaaatcca
     3061 gtactaaaat ccagatcgct gcaagaattc aagcttggag ccaacactcg aatccccacc
     3121 cgttggacca aacccggctc attaagcgtc ggttcagatt tatttccttt atttaaaaaa
     3181 aaggaaaggg taaaaaatag aaaattggaa acagttaaag cccaaaattg taatttaccg
     3241 agaattgtaa atttacctga aaaccctacg ctatagtttc gactataaat accaaactta
     3301 ggacctcact tcagaatccc ctcgtcgctg cgtctctctc ccgcaacctt cgattttcgt
     3361 ttattcgcat ccatcggaga gagaaaacaa tcaataagcg acctactaca aacaagtaag
     3421 taagagaaaa accaaaagaa gaagagaaac aacaagaaga agtaatggag gacgccaaaa
     3481 acataaagaa aggcccggcg ccattctatc ctctagagga tggaaccgct ggagagcaac
     3541 tgcataaggc tatgaagaga tacgccctgg ttcctggaac aattgctttt acagatgcac
     3601 atatcgaggt gaacatcacg tacgcggaat acttcgaaat gtccgttcgg ttggcagaag
     3661 ctatgaaacg atatgggctg aatacaaatc acagaatcgt cgtatgcagt gaaaactctc
     3721 ttcaattctt tatgccggtg ttgggcgcgt tatttatcgg agttgcagtt gcgcccgcga
     3781 acgacattta taatgaacgt gaattgctca acagtatgaa catttcgcag cctaccgtag
     3841 tgtttgtttc caaaaagggg ttgcaaaaaa ttttgaacgt gcaaaaaaaa ttaccaataa
     3901 tccagaaaat tattatcatg gattctaaaa cggattacca gggatttcag tcgatgtaca
     3961 cgttcgtcac atctcatcta cctcccggtt ttaatgaata cgattttgta ccagagtcct
     4021 ttgatcgtga caaaacaatt gcactgataa tgaattcctc tggatctact gggttaccta
     4081 agggtgtggc ccttccgcat agaactgcct gcgtcagatt ctcgcatgcc agagatccta
     4141 tttttggcaa tcaaatcatt ccggatactg cgattttaag tgttgttcca ttccatcacg
     4201 gttttggaat gtttactaca ctcggatatt tgatatgtgg atttcgagtc gtcttaatgt
     4261 atagatttga agaagagctg tttttacgat cccttcagga ttacaaaatt caaagtgcgt
     4321 tgctagtacc aaccctattt tcattcttcg ccaaaagcac tctgattgac aaatacgatt
     4381 tatctaattt acacgaaatt gcttctgggg gcgcacctct ttcgaaagaa gtcggggaag
     4441 cggttgcaaa acgcttccat cttccaggga tacgacaagg atatgggctc actgagacta
     4501 catcagctat tctgattaca cccgaggggg atgataaacc gggcgcggtc ggtaaagttg
     4561 ttccattttt tgaagcgaag gttgtggatc tggataccgg gaaaacgctg ggcgttaatc
     4621 agagaggcga attatgtgtc agaggaccta tgattatgtc cggttatgta aacaatccgg
     4681 aagcgaccaa cgccttgatt gacaaggatg gatggctaca ttctggagac atagcttact
     4741 gggacgagga cgaacacttc ttcatagttg accgcttgaa gtctttaatt aaatacaaag
     4801 gatatcaggt ggcccccgct gaattggaat cgatattgtt acaacacccc aacatcttcg
     4861 acgcgggcgt ggcaggtctg cccgacgatg acgccggtga acttcccgcc gccgttgttg
     4921 ttttggagca cggaaagacg atgacggaaa aagagatcgt ggattacgtc gccagtcaag
     4981 taacaaccgc gaaaaagttg cgcggaggag ttgtgtttgt ggacgaagta ccgaaaggtc
     5041 ttaccggaaa actcgacgca agaaaaatca gagagatcct cataaaggcc aagaagggcg
     5101 gaaagtccaa attgtaagct tgatcccccg tcgacagcta gctatatcat caatttatgt
     5161 attacacata atatcgcact cagtctttca tctacggcaa tgtaccagct gatataatca
     5221 gttattgaaa tatttctgaa tttaaacttg catcaataaa tttatgtttt tgcttggact
     5281 ataatacctg acttgttatt ttatcaataa atatttaaac tatatttctt tcaagatcgc
     5341 tactagaatt cgagctcgga gtcgacaaaa tttagaacga acttaattat gatctcaaat
     5401 acattgatac atatctcatc tagatctagg ttatcattat gtaagaaagt tttgacgaat
     5461 atggcacgac aaaatggcta gactcgatgt aattggtatc tcaactcaac attatactta
     5521 taccaaacat tagttagaca aaatttaaac aactattttt tatgtatgca agagtcagca
     5581 tatgtataat tgattcagaa tcgttttgac gagttcggat gtagtagtag ccattattta
     5641 atgtacatac taatcgtgaa tagtgaatat gatgaaacat tgtatcttat tgtataaata
     5701 tccataaaca catcatgaaa gacactttct ttcacggtct gaattaatta tgatacaatt
     5761 ctaatagaaa acgaattaaa ttacgttgaa ttgtatgaaa tctaattgaa caagccaacc
     5821 acgacgacga ctaacgttgc ctggattgac tcggtttaag ttaaccacta aaaaaacgga
     5881 gctgtcatgt aacacgcgga tcgagcaggt cacagtcatg aagccatcaa agcaaaagaa
     5941 ctaatccaag ggctgagatg attaattagt ttaaaaatta gttaacacga gggaaaaggc
     6001 tgtctgacag ccaggtcacg ttatctttac ctgtggtcga aatgattcgt gtctgtcgat
     6061 tttaattatt tttttgaaag gccgaaaata aagttgtaag agataaaccc gcctatataa
     6121 attcatatat tttcctctcc gctttgaata ctgtattttt acaacaatta ccaacaacaa
     6181 caaacaacaa acaacattac aattactatt tacaattaca atggctccta agaagaagag
     6241 gaaggtgatg agccagttcg acatcctgtg caagaccccc cccaaggtgc tggtgcggca
     6301 gttcgtggag agattcgaga ggcccagcgg cgagaagatc gccagctgtg ccgccgagct
     6361 gacctacctg tgctggatga tcacccacaa cggcaccgcc atcaagaggg ccaccttcat
     6421 gagctacaac accatcatca gcaacagcct gagcttcgac atcgtgaaca agagcctgca
     6481 gttcaagtac aagacccaga aggccaccat cctggaggcc agcctgaaga agctgatccc
     6541 cgcctgggag ttcaccatca tcccttacaa cggccagaag caccagagcg acatcaccga
     6601 catcgtgtcc agcctgcagc tgcagttcga gagcagcgag gaggccgaca agggcaacag
     6661 ccacagcaag aagatgctga aggccctgct gtccgagggc gagagcatct gggagatcac
     6721 cgagaagatc ctgaacagct tcgagtacac cagcaggttc accaagacca agaccctgta
     6781 ccagttcctg ttcctggcca cattcatcaa ctgcggcagg ttcagcgaca tcaagaacgt
     6841 ggaccccaag agcttcaagc tggtgcagaa caagtacctg ggcgtgatca ttcagtgcct
     6901 ggtgaccgag actaagacaa gcgtgtccag gtaagtttct gcttctacct ttgatatata
     6961 tataataatt atcattaatt agtagtaata taatatttca aatatttttt tcaaaataaa
     7021 agaatgtagt atatagcaat tgcttttctg tagtttataa gtgtgtatat tttaatttat
     7081 aacttttcta atatatgacc aaaatttgtt gatgtgcagg cacatctact ttttcagcgc
     7141 cagaggcagg atcgaccccc tggtgtacct ggacgagttc ctgaggaaca gcgagcccgt
     7201 gctgaagaga gtgaacagga ccggcaacag cagcagcaac aagcaggagt accagctgct
     7261 gaaggacaac ctggtgcgca gctacaacaa ggccctgaag aagaacgccc cctaccccat
     7321 cttcgctatc aagaacggcc ctaagagcca catcggcagg cacctgatga ccagctttct
     7381 gagcatgaag ggcctgaccg agctgacaaa cgtggtgggc aactggagcg acaagagggc
     7441 ctccgccgtg gccaggacca cctacaccca ccagatcacc gccatccccg accactactt
     7501 cgccctggtg tccaggtact acgcctacga ccccatcagc aaggagatga tcgccctgaa
     7561 ggacgagact aaccccatcg aggagtggca gcacatcgag cagctgaagg gcagcgccga
     7621 gggcagcatc agataccccg cctggaacgg catcatcagc caggaggtgc tggactacct
     7681 gagcagctac atcaacaggc ggatctgagc ttctctagct agagtcgatc gacaagctcg
     7741 agtttctcca taataatgtg tgagtagttc ccagataagg gaattagggt tcctataggg
     7801 tttcgctcat gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac
     7861 ttctatcaat aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatcgct
     7921 ttactgagac cgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     7981 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     8041 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     8101 ccatgattac gccaagcttg catgcctgca ggtcgactct agaggatccc cgggtaccga
     8161 gctcgaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     8221 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     8281 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt
     8341 attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa
     8401 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
     8461 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacg gtctcaggga
     8521 cacgaagtga tccgtttaaa ctatcagtgt ttgacaggat atattggcgg gtaaacctaa
     8581 gagaaaagag cgtttattag aataatcgga tatttaaaag ggcgtgaaaa ggtttatccg
     8641 ttcgtccatt tgtatgtgcc agccgccttt gcgacgctca ccgggctggt tgccctcgcc
     8701 gctgggctgg cggccgtcta tggccctgca aacgcgccag aaacgccgtc gaagccgtgt
     8761 gcgagacacc gcggccgccg gcgttgtgga tacctcgcgg aaaacttggc cctcactgac
     8821 agatgagggg cggacgttga cacttgaggg gccgactcac ccggcgcggc gttgacagat
     8881 gaggggcagg ctcgatttcg gccggcgacg tggagctggc cagcctcgca aatcggcgaa
     8941 aacgcctgat tttacgcgag tttcccacag atgatgtgga caagcctggg gataagtgcc
     9001 ctgcggtatt gacacttgag gggcgcgact actgacagat gaggggcgcg atccttgaca
     9061 cttgaggggc agagtgctga cagatgaggg gcgcacctat tgacatttga ggggctgtcc
     9121 acaggcagaa aatccagcat ttgcaagggt ttccgcccgt ttttcggcca ccgctaacct
     9181 gtcttttaac ctgcttttaa accaatattt ataaaccttg tttttaacca gggctgcgcc
     9241 ctgtgcgcgt gaccgcgcac gccgaagggg ggtgcccccc cttctcgaac cctcccggcc
     9301 cgctaacgcg ggcctcccat ccccccaggg gctgcgcccc tcggccgcga acggcctcac
     9361 cccaaaaatg gcagcgctgg ccaattcccg agtgcgcgga acccctattt gtttattttt
     9421 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     9481 atattgaaaa aggaagagta tggctaaaat gagaatatca ccggaattga aaaaactgat
     9541 cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg tatataagct
     9601 ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata aagggaccac
     9661 ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc tgcctgttcc
     9721 aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga gtgaggccga
     9781 tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga ttatcgagct
     9841 gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc cctatacgaa
     9901 tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc tggccgatgt
     9961 ggattgcgaa aactgggaag aggacactcc atttaaagat ccgcgcgagc tgtatgattt
    10021 tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc tgggagacag
    10081 caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga gaagcggcag
    10141 ggcggacaag tggtatgaca ttgccttctg cgtccggtcg ctcagggagg atatcgggga
    10201 agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt gggagaaaat
    10261 aaaatattat attttactgg atgaattgtt ttagctgtca gaccaagttt actcatatat
    10321 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
    10381 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
    10441 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
    10501 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
    10561 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
    10621 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
    10681 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
    10741 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
    10801 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
    10861 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
    10921 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
    10981 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
    11041 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcag atcctagatg
    11101 tggcgcaacg atgccggcga caagcaggag cgcaccgact tcttccgcat caagtgtttt
    11161 ggctctcagg ccgaggccca cggcaagtat ttgggcaagg ggtcgctggt attcgtgcag
    11221 ggcaagattc ggaataccaa gtacgagaag gacggccaga cggtctacgg gaccgacttc
    11281 attgccgata aggtggatta tctggacacc aaggcaccag gcgggtcaaa tcaggaataa
    11341 gggcacattg ccccggcgtg agtcggggca atcccgcaag gagggtgaat gaatcggacg
    11401 tttgaccgga aggcatacag gcaagaactg atcgacgcgg ggttttccgc cgaggatgcc
    11461 gaaaccatcg caagccgcac cgtcatgcgt gcgccccgcg aaaccttcca gtccgtcggc
    11521 tcgatggtcc agcaagctac ggccaagatc gagcgcgaca gcgtgcaact ggctccccct
    11581 gccctgcccg cgccatcggc cgccgtggag cgttcgcgtc gtcttgaaca ggaggcggca
    11641 ggtttggcga agtcgatgac catcgacacg cgaggaacta tgacgaccaa gaagcgaaaa
    11701 accgccggcg aggacctggc aaaacaggtc agcgaggcca agcaggccgc gttgctgaaa
    11761 cacacgaagc agcagatcaa ggaaatgcag ctttccttgt tcgatattgc gccgtggccg
    11821 gacacgatgc gagcgatgcc aaacgacacg gcccgctctg ccctgttcac cacgcgcaac
    11881 aagaaaatcc cgcgcgaggc gctgcaaaac aaggtcattt tccacgtcaa caaggacgtg
    11941 aagatcacct acaccggcgt cgagctgcgg gccgacgatg acgaactggt gtggcagcag
    12001 gtgttggagt acgcgaagcg cacccctatc ggcgagccga tcaccttcac gttctacgag
    12061 ctttgccagg acctgggctg gtcgatcaat ggccggtatt acacgaaggc cgaggaatgc
    12121 ctgtcgcgcc tacaggcgac ggcgatgggc ttcacgtccg accgcgttgg gcacctggaa
    12181 tcggtgtcgc tgctgcaccg cttccgcgtc ctggaccgtg gcaagaaaac gtcccgttgc
    12241 caggtcctga tcgacgagga aatcgtcgtg ctgtttgctg gcgaccacta cacgaaattc
    12301 atatgggaga agtaccgcaa gctgtcgccg acggcccgac ggatgttcga ctatttcagc
    12361 tcgcaccggg agccgtaccc gctcaagctg gaaaccttcc gcctcatgtg cggatcggat
    12421 tccacccgcg tgaagaagtg gcgcgagcag gtcggcgaag cctgcgaaga gttgcgaggc
    12481 agcggcctgg tggaacacgc ctgggtcaat gatgacctgg tgcattgcaa acgctagggc
    12541 cttgtggggt cagttccggc tgggggttca gcagcccctg ctcggatctg ttggaccgga
    12601 cagtagtcat ggttgatggg ctgcctgtat cgagtggtga ttttgtgccg agctgccggt
    12661 cggggagctg ttggctggct ggtggcagga tatattgtgg tgtaaacaaa ttgacgctta
    12721 gacaacttaa taacacattg cggacgtttt taatgtactg gggttgaaca ctct
//
