LOCUS       sequence-377963-       12572 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To act as a 1-input state of the split-AND gate..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12572)
  AUTHORS   Lloyd JPB, Ly F, Gong P, Pflueger J, Swain T, Pflueger C, Fourie E, 
            Khan MA, Kidd BN, Lister R
  TITLE     Synthetic memory circuits for stable cell reprogramming in plants.
  JOURNAL   Nat Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2.
  PUBMED    35788565
REFERENCE   2  (bases 1 to 12572)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2."
COMMENT     Sequence Label: LLP690_L2_1IF1_FRT-OCS_Rluc_FlpF1_C1-NLS
FEATURES             Location/Qualifiers
     source          1..12572
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    845..878
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     terminator      879..1586
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     protein_bind    1587..1620
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     intron          1687..1875
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     polyA_signal    2903..3077
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             3465..5117
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(3531..3549)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      5137..5337
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     misc_feature    5680..5735
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     intron          6420..6608
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     CDS             7483..7503
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7541..7715
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     protein_bind    7785..7806
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7821..7826,7827..7844,7845..7851)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7859..7875
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7864..7886
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7883..7899
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7883..7899
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             7895..8218
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    7912..7968
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(7969..7986)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7969..7985)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7978..8000)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8194..8213)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    8350..8374
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      8462..9172
                     /label=oriV
                     /note="incP origin of replication"
     promoter        9193..9297
                     /gene="bla"
                     /label=AmpR promoter
     CDS             9298..10092
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      10263..10851
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10752..10771
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             11187..12335
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    12481..12505
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgaatt cggatccgga gtcgacaaaa tttagaacga acttaattat gatctcaaat
       61 acattgatac atatctcatc tagatctagg ttatcattat gtaagaaagt tttgacgaat
      121 atggcacgac aaaatggcta gactcgatgt aattggtatc tcaactcaac attatactta
      181 taccaaacat tagttagaca aaatttaaac aactattttt tatgtatgca agagtcagca
      241 tatgtataat tgattcagaa tcgttttgac gagttcggat gtagtagtag ccattattta
      301 atgtacatac taatcgtgaa tagtgaatat gatgaaacat tgtatcttat tgtataaata
      361 tccataaaca catcatgaaa gacactttct ttcacggtct gaattaatta tgatacaatt
      421 ctaatagaaa acgaattaaa ttacgttgaa ttgtatgaaa tctaattgaa caagccaacc
      481 acgacgacga ctaacgttgc ctggattgac tcggtttaag ttaaccacta aaaaaacgga
      541 gctgtcatgt aacacgcgga tcgagcaggt cacagtcatg aagccatcaa agcaaaagaa
      601 ctaatccaag ggctgagatg attaattagt ttaaaaatta gttaacacga gggaaaaggc
      661 tgtctgacag ccaggtcacg ttatctttac ctgtggtcga aatgattcgt gtctgtcgat
      721 tttaattatt tttttgaaag gccgaaaata aagttgtaag agataaaccc gcctatataa
      781 attcatatat tttcctctcc gctttgaata ctgtattttt acaacaatta ccaacaacaa
      841 caaagaagtt cctattctct agaaagtata ggaacttcct gctttaatga gatatgcgag
      901 aagcctatga tcgcatgata tttgctttca attctgttgt gcacgttgta aaaaacctga
      961 gcatgtgtag ctcagatcct taccgccggt ttcggttcat tctaatgaat atatcacccg
     1021 ttactatcgt atttttatga ataatattct ccgttcaatt tactgattgt accctactac
     1081 ttatatgtac aatattaaaa tgaaaacaat atattgtgct gaataggttt atagcgacat
     1141 ctatgataga gcgccacaat aacaaacaat tgcgttttat tattacaaat ccaattttaa
     1201 aaaaagcggc agaaccggtc aaacctaaaa gactgattac ataaatctta ttcaaatttc
     1261 aaaagtgccc caggggctag tatctacgac acaccgagcg gcgaactaat aacgctcact
     1321 gaagggaact ccggttcccc gccggcgcgc atgggtgaga ttccttgaag ttgagtattg
     1381 gccgtccgct ctaccgaaag ttacgggcac cattcaaccc ggtccagcac ggcggccggg
     1441 taaccgactt gctgccccga gaattatgca gcattttttt ggtgtatgtg ggccccaaat
     1501 gaagtgcagg tcaaaccttg acagtgacga caaatcgttg ggcgggtcca gggcgaattt
     1561 tgcgacaaca tgtcgaggct cagcaggaag ttcctattct ctagaaagta taggaacttc
     1621 caacaaacaa cattacaatt actatttaca attacaatga cttcaaaggt gtatgatcct
     1681 gagcaggtaa gtttctgctt ctacctttga tatatatata ataattatca ttaattagta
     1741 gtaatataat atttcaaata tttttttcaa aataaaagaa tgtagtatat agcaattgct
     1801 tttctgtagt ttataagtgt gtatatttta atttataact tttctaatat atgaccaaaa
     1861 tttgttgatg tgcagagaaa aagaatgata actggtccac aatggtgggc aagatgtaaa
     1921 cagatgaacg tgcttgattc tttcattaat tactacgatt cagaaaagca cgctgagaat
     1981 gcagttatct ttcttcacgg aaacgctgca tcttcatatt tgtggagaca tgttgtgcct
     2041 cacattgaac cagtggctag gtgtattatc ccagatctta tcggaatggg aaagtctggt
     2101 aaatctggaa atggttctta cagacttttg gatcattata agtacctcac agcttggttc
     2161 gaacttctca acttacctaa gaagattatt ttcgttggac acgattgggg tgcttgcttg
     2221 gcattccatt actcatacga acaccaagat aagatcaaag ctatagttca tgcagagagt
     2281 gttgtggatg tgatagaatc ttgggatgag tggccagata ttgaagagga tattgctttg
     2341 atcaagtctg aagagggaga aaagatggtt ctcgaaaata actttttcgt ggaaactatg
     2401 cttccttcaa agattatgag aaaattggaa ccagaagagt ttgctgcata tctcgaacct
     2461 ttcaaggaaa aaggagaggt tagaaggcca acactcagtt ggcctaggga aataccatta
     2521 gttaagggag gtaaacctga tgttgtgcag atcgtgagaa attataacgc ttaccttagg
     2581 gcatcagatg atttgcctaa gatgttcatc gagtcagatc caggattttt cagtaatgct
     2641 atagttgaag gtgcaaagaa atttccaaac accgagttcg ttaaggtgaa aggattgcat
     2701 ttctcacaag aagatgctcc tgatgagatg ggaaagtata ttaagtcttt tgttgaaaga
     2761 gtgctcaaaa acgagcaggg ttctcgatct gatgcttctg aagtaacgtc aacaagctcc
     2821 cagtctgagg tatgcacagt ggaaacgcca ggttgtgttg gttcttaagc ttctctagct
     2881 agagtcgatc gacaagctcg agtttctcca taataatgtg tgagtagttc ccagataagg
     2941 gaattagggt tcctataggg tttcgctcat gtgttgagca tataagaaac ccttagtatg
     3001 tatttgtatt tgtaaaatac ttctatcaat aaaatttcta attcctaaaa ccaaaatcca
     3061 gtactaaaat ccagatcgct gcaagaattc aagcttggag ccaacactcg aatccccacc
     3121 cgttggacca aacccggctc attaagcgtc ggttcagatt tatttccttt atttaaaaaa
     3181 aaggaaaggg taaaaaatag aaaattggaa acagttaaag cccaaaattg taatttaccg
     3241 agaattgtaa atttacctga aaaccctacg ctatagtttc gactataaat accaaactta
     3301 ggacctcact tcagaatccc ctcgtcgctg cgtctctctc ccgcaacctt cgattttcgt
     3361 ttattcgcat ccatcggaga gagaaaacaa tcaataagcg acctactaca aacaagtaag
     3421 taagagaaaa accaaaagaa gaagagaaac aacaagaaga agtaatggag gacgccaaaa
     3481 acataaagaa aggcccggcg ccattctatc ctctagagga tggaaccgct ggagagcaac
     3541 tgcataaggc tatgaagaga tacgccctgg ttcctggaac aattgctttt acagatgcac
     3601 atatcgaggt gaacatcacg tacgcggaat acttcgaaat gtccgttcgg ttggcagaag
     3661 ctatgaaacg atatgggctg aatacaaatc acagaatcgt cgtatgcagt gaaaactctc
     3721 ttcaattctt tatgccggtg ttgggcgcgt tatttatcgg agttgcagtt gcgcccgcga
     3781 acgacattta taatgaacgt gaattgctca acagtatgaa catttcgcag cctaccgtag
     3841 tgtttgtttc caaaaagggg ttgcaaaaaa ttttgaacgt gcaaaaaaaa ttaccaataa
     3901 tccagaaaat tattatcatg gattctaaaa cggattacca gggatttcag tcgatgtaca
     3961 cgttcgtcac atctcatcta cctcccggtt ttaatgaata cgattttgta ccagagtcct
     4021 ttgatcgtga caaaacaatt gcactgataa tgaattcctc tggatctact gggttaccta
     4081 agggtgtggc ccttccgcat agaactgcct gcgtcagatt ctcgcatgcc agagatccta
     4141 tttttggcaa tcaaatcatt ccggatactg cgattttaag tgttgttcca ttccatcacg
     4201 gttttggaat gtttactaca ctcggatatt tgatatgtgg atttcgagtc gtcttaatgt
     4261 atagatttga agaagagctg tttttacgat cccttcagga ttacaaaatt caaagtgcgt
     4321 tgctagtacc aaccctattt tcattcttcg ccaaaagcac tctgattgac aaatacgatt
     4381 tatctaattt acacgaaatt gcttctgggg gcgcacctct ttcgaaagaa gtcggggaag
     4441 cggttgcaaa acgcttccat cttccaggga tacgacaagg atatgggctc actgagacta
     4501 catcagctat tctgattaca cccgaggggg atgataaacc gggcgcggtc ggtaaagttg
     4561 ttccattttt tgaagcgaag gttgtggatc tggataccgg gaaaacgctg ggcgttaatc
     4621 agagaggcga attatgtgtc agaggaccta tgattatgtc cggttatgta aacaatccgg
     4681 aagcgaccaa cgccttgatt gacaaggatg gatggctaca ttctggagac atagcttact
     4741 gggacgagga cgaacacttc ttcatagttg accgcttgaa gtctttaatt aaatacaaag
     4801 gatatcaggt ggcccccgct gaattggaat cgatattgtt acaacacccc aacatcttcg
     4861 acgcgggcgt ggcaggtctg cccgacgatg acgccggtga acttcccgcc gccgttgttg
     4921 ttttggagca cggaaagacg atgacggaaa aagagatcgt ggattacgtc gccagtcaag
     4981 taacaaccgc gaaaaagttg cgcggaggag ttgtgtttgt ggacgaagta ccgaaaggtc
     5041 ttaccggaaa actcgacgca agaaaaatca gagagatcct cataaaggcc aagaagggcg
     5101 gaaagtccaa attgtaagct tgatcccccg tcgacagcta gctatatcat caatttatgt
     5161 attacacata atatcgcact cagtctttca tctacggcaa tgtaccagct gatataatca
     5221 gttattgaaa tatttctgaa tttaaacttg catcaataaa tttatgtttt tgcttggact
     5281 ataatacctg acttgttatt ttatcaataa atatttaaac tatatttctt tcaagatcgc
     5341 tactagaatt cgagctcgga gccaacactc gaatccccac ccgttggacc aaacccggct
     5401 cattaagcgt cggttcagat ttatttcctt tatttaaaaa aaaggaaagg gtaaaaaata
     5461 gaaaattgga aacagttaaa gcccaaaatt gtaatttacc gagaattgta aatttacctg
     5521 aaaaccctac gctatagttt cgactataaa taccaaactt aggacctcac ttcagaatcc
     5581 cctcgtcgct gcgtctctct cccgcaacct tcgattttcg tttattcgca tccatcggag
     5641 agagaaaaca atcaataagc gacctactgt atttttacaa caattaccaa caacaacaaa
     5701 caacaaacaa cattacaatt actatttaca attacaatga gccagttcga catcctgtgc
     5761 aagacccccc ccaaggtgct ggtgcggcag ttcgtggaga gattcgagag gcccagcggc
     5821 gagaagatcg ccagctgtgc cgccgagctg acctacctgt gctggatgat cacccacaac
     5881 ggcaccgcca tcaagagggc caccttcatg agctacaaca ccatcatcag caacagcctg
     5941 agcttcgaca tcgtgaacaa gagcctgcag ttcaagtaca agacccagaa ggccaccatc
     6001 ctggaggcca gcctgaagaa gctgatcccc gcctgggagt tcaccatcat cccttacaac
     6061 ggccagaagc accagagcga catcaccgac atcgtgtcca gcctgcagct gcagttcgag
     6121 agcagcgagg aggccgacaa gggcaacagc cacagcaaga agatgctgaa ggccctgctg
     6181 tccgagggcg agagcatctg ggagatcacc gagaagatcc tgaacagctt cgagtacacc
     6241 agcaggttca ccaagaccaa gaccctgtac cagttcctgt tcctggccac attcatcaac
     6301 tgcggcaggt tcagcgacat caagaacgtg gaccccaaga gcttcaagct ggtgcagaac
     6361 aagtacctgg gcgtgatcat tcagtgcctg gtgaccgaga ctaagacaag cgtgtccagg
     6421 taagtttctg cttctacctt tgatatatat ataataatta tcattaatta gtagtaatat
     6481 aatatttcaa atattttttt caaaataaaa gaatgtagta tatagcaatt gcttttctgt
     6541 agtttataag tgtgtatatt ttaatttata acttttctaa tatatgacca aaatttgttg
     6601 atgtgcaggc acatctactt tttcagcgcc agaggcagga tcgaccccct ggtgtacctg
     6661 gacgagttcc tgaggaacag cgagcccgtg ctgaagagag tgaacaggac cggcaacagc
     6721 agcagcaaca agcaggagta ccagctgctg aaggacaacc tggtgcgcag ctacaacaag
     6781 gccctgaaga agaacgcccc ctaccccatc ttcgctatca agaacggccc taagagccac
     6841 atcggcaggc acctgatgac cagctttctg agcatgaagg gcctgaccga gctgacaaac
     6901 gtggtgggca actggagcga caagagggcc tccgccgtgg ccaggaccac ctacacccac
     6961 cagatcaccg ccatccccga ccactacttc gccctggtgt ccaggtacta cgcctacgac
     7021 cccatcagca aggagatgat cgccctgaag gacgagacta accccatcga ggagtggcag
     7081 cacatcgagc agctgaaggg cagcgccgag ggcagcctct ccggagggtc tggctccgga
     7141 tcaagtggtg gcagcggtac ttcgagcaca accaaaaaag ccagtgattc tgcattctgg
     7201 cttgaggttg aaggtaattc catgaccgca ccaacaggct ccaagccaag ctttcctgac
     7261 ggaatgttaa ttctcgttga ccctgagcag gctgttgagc caggtgattt ctgcatagcc
     7321 agacttgggg gtgatgagtt taccttcaag aaactgatca gggatagcgg tcaggtgttt
     7381 ttacaaccac taaacccaca gtacccaatg atcccatgca atgagagttg ttccgttgtg
     7441 gggaaagtta tcgctagtca gtggcctgaa gagacgtttg gccccaagaa aaagcggaag
     7501 gtgtaggctt ctctagctag agtcgatcga caagctcgag tttctccata ataatgtgtg
     7561 agtagttccc agataaggga attagggttc ctatagggtt tcgctcatgt gttgagcata
     7621 taagaaaccc ttagtatgta tttgtatttg taaaatactt ctatcaataa aatttctaat
     7681 tcctaaaacc aaaatccagt actaaaatcc agatcgcttt actgagaccg cagctggcac
     7741 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     7801 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     7861 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagcttgca
     7921 tgcctgcagg tcgactctag aggatccccg ggtaccgagc tcgaattcac tggccgtcgt
     7981 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     8041 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     8101 gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg
     8161 cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     8221 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     8281 ggcatccgct tacagacaag ctgtgacggt ctcagggaca cgaagtgatc cgtttaaact
     8341 atcagtgttt gacaggatat attggcgggt aaacctaaga gaaaagagcg tttattagaa
     8401 taatcggata tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg tatgtgccag
     8461 ccgcctttgc gacgctcacc gggctggttg ccctcgccgc tgggctggcg gccgtctatg
     8521 gccctgcaaa cgcgccagaa acgccgtcga agccgtgtgc gagacaccgc ggccgccggc
     8581 gttgtggata cctcgcggaa aacttggccc tcactgacag atgaggggcg gacgttgaca
     8641 cttgaggggc cgactcaccc ggcgcggcgt tgacagatga ggggcaggct cgatttcggc
     8701 cggcgacgtg gagctggcca gcctcgcaaa tcggcgaaaa cgcctgattt tacgcgagtt
     8761 tcccacagat gatgtggaca agcctgggga taagtgccct gcggtattga cacttgaggg
     8821 gcgcgactac tgacagatga ggggcgcgat ccttgacact tgaggggcag agtgctgaca
     8881 gatgaggggc gcacctattg acatttgagg ggctgtccac aggcagaaaa tccagcattt
     8941 gcaagggttt ccgcccgttt ttcggccacc gctaacctgt cttttaacct gcttttaaac
     9001 caatatttat aaaccttgtt tttaaccagg gctgcgccct gtgcgcgtga ccgcgcacgc
     9061 cgaagggggg tgccccccct tctcgaaccc tcccggcccg ctaacgcggg cctcccatcc
     9121 ccccaggggc tgcgcccctc ggccgcgaac ggcctcaccc caaaaatggc agcgctggcc
     9181 aattcccgag tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     9241 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     9301 gctaaaatga gaatatcacc ggaattgaaa aaactgatcg aaaaataccg ctgcgtaaaa
     9361 gatacggaag gaatgtctcc tgctaaggta tataagctgg tgggagaaaa tgaaaaccta
     9421 tatttaaaaa tgacggacag ccggtataaa gggaccacct atgatgtgga acgggaaaag
     9481 gacatgatgc tatggctgga aggaaagctg cctgttccaa aggtcctgca ctttgaacgg
     9541 catgatggct ggagcaatct gctcatgagt gaggccgatg gcgtcctttg ctcggaagag
     9601 tatgaagatg aacaaagccc tgaaaagatt atcgagctgt atgcggagtg catcaggctc
     9661 tttcactcca tcgacatatc ggattgtccc tatacgaata gcttagacag ccgcttagcc
     9721 gaattggatt acttactgaa taacgatctg gccgatgtgg attgcgaaaa ctgggaagag
     9781 gacactccat ttaaagatcc gcgcgagctg tatgattttt taaagacgga aaagcccgaa
     9841 gaggaacttg tcttttccca cggcgacctg ggagacagca acatctttgt gaaagatggc
     9901 aaagtaagtg gctttattga tcttgggaga agcggcaggg cggacaagtg gtatgacatt
     9961 gccttctgcg tccggtcgct cagggaggat atcggggaag aacagtatgt cgagctattt
    10021 tttgacttac tggggatcaa gcctgattgg gagaaaataa aatattatat tttactggat
    10081 gaattgtttt agctgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
    10141 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
    10201 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
    10261 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
    10321 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
    10381 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
    10441 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
    10501 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
    10561 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
    10621 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
    10681 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
    10741 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
    10801 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
    10861 aacgcggcct ttttacggtt cctggcagat cctagatgtg gcgcaacgat gccggcgaca
    10921 agcaggagcg caccgacttc ttccgcatca agtgttttgg ctctcaggcc gaggcccacg
    10981 gcaagtattt gggcaagggg tcgctggtat tcgtgcaggg caagattcgg aataccaagt
    11041 acgagaagga cggccagacg gtctacggga ccgacttcat tgccgataag gtggattatc
    11101 tggacaccaa ggcaccaggc gggtcaaatc aggaataagg gcacattgcc ccggcgtgag
    11161 tcggggcaat cccgcaagga gggtgaatga atcggacgtt tgaccggaag gcatacaggc
    11221 aagaactgat cgacgcgggg ttttccgccg aggatgccga aaccatcgca agccgcaccg
    11281 tcatgcgtgc gccccgcgaa accttccagt ccgtcggctc gatggtccag caagctacgg
    11341 ccaagatcga gcgcgacagc gtgcaactgg ctccccctgc cctgcccgcg ccatcggccg
    11401 ccgtggagcg ttcgcgtcgt cttgaacagg aggcggcagg tttggcgaag tcgatgacca
    11461 tcgacacgcg aggaactatg acgaccaaga agcgaaaaac cgccggcgag gacctggcaa
    11521 aacaggtcag cgaggccaag caggccgcgt tgctgaaaca cacgaagcag cagatcaagg
    11581 aaatgcagct ttccttgttc gatattgcgc cgtggccgga cacgatgcga gcgatgccaa
    11641 acgacacggc ccgctctgcc ctgttcacca cgcgcaacaa gaaaatcccg cgcgaggcgc
    11701 tgcaaaacaa ggtcattttc cacgtcaaca aggacgtgaa gatcacctac accggcgtcg
    11761 agctgcgggc cgacgatgac gaactggtgt ggcagcaggt gttggagtac gcgaagcgca
    11821 cccctatcgg cgagccgatc accttcacgt tctacgagct ttgccaggac ctgggctggt
    11881 cgatcaatgg ccggtattac acgaaggccg aggaatgcct gtcgcgccta caggcgacgg
    11941 cgatgggctt cacgtccgac cgcgttgggc acctggaatc ggtgtcgctg ctgcaccgct
    12001 tccgcgtcct ggaccgtggc aagaaaacgt cccgttgcca ggtcctgatc gacgaggaaa
    12061 tcgtcgtgct gtttgctggc gaccactaca cgaaattcat atgggagaag taccgcaagc
    12121 tgtcgccgac ggcccgacgg atgttcgact atttcagctc gcaccgggag ccgtacccgc
    12181 tcaagctgga aaccttccgc ctcatgtgcg gatcggattc cacccgcgtg aagaagtggc
    12241 gcgagcaggt cggcgaagcc tgcgaagagt tgcgaggcag cggcctggtg gaacacgcct
    12301 gggtcaatga tgacctggtg cattgcaaac gctagggcct tgtggggtca gttccggctg
    12361 ggggttcagc agcccctgct cggatctgtt ggaccggaca gtagtcatgg ttgatgggct
    12421 gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt ggctggctgg
    12481 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
    12541 gacgttttta atgtactggg gttgaacact ct
//
