LOCUS       sequence-377964-        4029 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express the bacterial monomeric sarcosine oxidase enzyme under the 
            control of the strong constitutif phage promoter T7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4029)
  AUTHORS   Soudier P, Zuniga A, Duigou T, Voyvodic PL, Bazi-Kabbaj K, Kushwaha 
            M, Vendrell JA, Solassol J, Bonnet J, Faulon JL
  TITLE     PeroxiHUB: A Modular Cell-Free Biosensing Platform Using H(2)O(2) as
            Signal Integrator.
  JOURNAL   ACS Synth Biol. 2022 Aug 19;11(8):2578-2588. doi: 
            10.1021/acssynbio.2c00138. Epub 2022 Aug 1.
  PUBMED    35913043
REFERENCE   2  (bases 1 to 4029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.2c00138"; journalName: "ACS Synth Biol"; date: 
            "2022-08-19- 19"; volume: "11"; issue: "8"; pages: "2578-2588"
COMMENT     Sequence Label: pBeast-pT7-soxA
FEATURES             Location/Qualifiers
     source          1..4029
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     5..24
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5..23
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             40..62
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(1573..1590)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1744..2332)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1824..1843)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2503..3294,3295..3363))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3126..3145
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3364..3468)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3536..3554
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      3906..3933
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 cttataatac gactcactat agggagagct agcaataatt ttgtttaact ttaagaagga
       61 gatataatgt caacacattt tgatgtgatc gttgtcgggg caggttcgat gggaatggct
      121 gctggatatc agttggcaaa acaaggagta aagacactgt tggtagacgc ttttgacccc
      181 ccgcacacaa atgggtctca tcatggcgac acacgtatta ttcgccacgc atatggggaa
      241 ggacgtgagt atgtaccatt ggccttgcgt tcacaggagt tatggtacga gttggagaag
      301 gagacccacc acaagatctt cacaaaaaca ggcgttttag tttttggacc gaaaggggaa
      361 agcgctttcg ttgccgagac aatggaagcc gcgaaagagc attctttaac cgtagacctg
      421 ctggaggggg acgagatcaa taagcgctgg cccggtatca cggtccccga gaactataac
      481 gctatctttg aaccaaattc tggtgtcctt ttttcggaga attgcattcg tgcttaccgc
      541 gagttagcag aagcccgtgg tgccaaagta ttgacacaca cgcgcgtgga agacttcgac
      601 atttcacccg attctgtcaa aattgaaacc gctaatggtt cctatacggc ggataagctt
      661 atcgttagca tgggcgcatg gaactcgaag ctgctgtcca aactgaactt ggatatccca
      721 ttacagccgt atcgccaagt cgtcggcttc tttgaaagcg atgaatccaa atatagcaac
      781 gatattgact ttccggggtt tatggtcgaa gtaccaaatg ggatttacta tggcttccct
      841 tccttcggtg gttgtgggct taagctgggt tatcatacgt ttggacagaa aatcgacccg
      901 gacacgatta accgtgaatt cggcgtatac ccagaagatg agtcaaacct tcgcgccttt
      961 cttgaagaat atatgcctgg tgccaacggg gaattaaaac gtggagccgt atgcatgtat
     1021 acgaagacat tagacgagca tttcatcatt gaccttcatc ccgaacattc gaatgtggtt
     1081 atcgccgctg ggtttagcgg ccacggcttc aaattcagca gcggggtggg agaggtactg
     1141 tcgcaattag ctttaactgg caaaacggaa catgatattt ccattttctc catcaaccgc
     1201 ccagccttga aggaatcctt gcagaagacc acaattactt tatctgagaa tagtcaatct
     1261 tcggaaatcc caggtggcat gctaaaagtc tcgtaaagcg ttctatcaat aacccgttgg
     1321 tgcaaagccc gccgaaaggc gggcttttct gtccgtctca gaatcggccg tgaacaataa
     1381 aatagtttcg gtattattga ccacttccga gtagaatcgt gcttcagtaa gagtcgaccg
     1441 atgcccttga gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc
     1501 gtcgccgcac ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg
     1561 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     1621 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     1681 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     1741 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     1801 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     1861 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     1921 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     1981 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     2041 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     2101 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     2161 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     2221 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     2281 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     2341 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     2401 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     2461 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     2521 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     2581 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     2641 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     2701 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     2761 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     2821 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     2881 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     2941 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     3001 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     3061 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac
     3121 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     3181 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     3241 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     3301 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     3361 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     3421 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     3481 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     3541 gcgtatcacg aggccctttc gtcttcaaga attctggcga atcctctgac cagccagaaa
     3601 acgacctttc tgtggtgaaa ccggatgctg caattcagag cgccagcaag tgggggacag
     3661 cagaagacct gaccgccgca gagtggatgt ttgacatggt gaagactatc gcaccatcag
     3721 ccagaaaacc gaattttgct gggtgggcta acgatatccg cctgatgcgt gaacgtgacg
     3781 gacgtaacca ccgcgacatg tgtgtgctgt tccgctggct cggataccct tactctgttg
     3841 aaaacgaata gataggttaa ggaacggtta tttctgcgta gatctatctt acacagcatc
     3901 acactggctc accttcgggt gggcctttct gcgtttatat actagagaga gaatataaaa
     3961 agccagatta ttaatccggc ttttttatta tttaggcaac tgaaacgatt cggatcctgt
     4021 attactatt
//
