LOCUS       sequence-377971-        4503 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express the bacterial choline oxidase enzyme under the control of 
            the strong constitutif phage promoter T7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4503)
  AUTHORS   Soudier P, Zuniga A, Duigou T, Voyvodic PL, Bazi-Kabbaj K, Kushwaha 
            M, Vendrell JA, Solassol J, Bonnet J, Faulon JL
  TITLE     PeroxiHUB: A Modular Cell-Free Biosensing Platform Using H(2)O(2) as
            Signal Integrator.
  JOURNAL   ACS Synth Biol. 2022 Aug 19;11(8):2578-2588. doi: 
            10.1021/acssynbio.2c00138. Epub 2022 Aug 1.
  PUBMED    35913043
REFERENCE   2  (bases 1 to 4503)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.2c00138"; journalName: "ACS Synth Biol"; date: 
            "2022-08-19- 19"; volume: "11"; issue: "8"; pages: "2578-2588"
COMMENT     Sequence Label: pBeast-pT7-codA
FEATURES             Location/Qualifiers
     source          1..4503
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     4..23
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4..22
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             39..61
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(2046..2063)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2217..2805)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2297..2316)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2976..3767,3768..3836))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3599..3618
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3837..3941)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4009..4027
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      4379..4406
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 ttataatacg actcactata gggagagcta gcaataattt tgtttaactt taagaaggag
       61 atataatgca catcgacaac atcgaaaacc tgtctgaccg tgaattcgac tacatcgttg
      121 ttggtggtgg ttctgctggt gctgctgttg ctgctcgtct gtctgaagac ccggctgttt
      181 ctgttgctct ggttgaagct ggtccggacg accgtggtgt tccggaagtt ctgcagctgg
      241 accgttggat ggaactgctg gaatctggtt acgactggga ctacccgatc gaaccgcagg
      301 aaaacggtaa ctctttcatg cgtcacgctc gtgctaaagt tatgggtggt tgctcttctc
      361 acaactcttg catcgctttc tgggctccgc gtgaagacct ggacgaatgg gaagctaaat
      421 acggtgctac cggttggaac gctgaagctg cttggccgct gtacaaacgt ctggaaacca
      481 acgaagacgc tggtccggac gctccgcacc acggtgactc tggtccggtt cacctgatga
      541 acgttccgcc gaaagacccg accggtgttg ctctgctgga cgcttgcgaa caggctggta
      601 tcccgcgtgc taaattcaac accggtacca ccgttgttaa cggtgctaac ttcttccaga
      661 tcaaccgtcg tgctgacggt acccgttctt cttcttctgt ttcttacatc cacccgatcg
      721 ttgaacagga aaacttcacc ctgctgaccg gtctgcgtgc tcgtcagctg gttttcgacg
      781 ctgaccgtcg ttgcaccggt gttgacatcg ttgactctgc tttcggtcac acccaccgtc
      841 tgaccgctcg taacgaagtt gttctgtcta ccggtgctat cgacaccccg aaactgctga
      901 tgctgtctgg tatcggtccg gctgctcacc tggctgaaca cggtatcgaa gttctggttg
      961 actctccggg tgttggtgaa cacctgcagg accacccgga aggtgttgtt cagttcgaag
     1021 ctaaacagcc gatggttgct gaatctaccc agtggtggga aatcggtatc ttcaccccga
     1081 ccgaagacgg tctggaccgt ccggacctga tgatgcacta cggttctgtt ccgttcgaca
     1141 tgaacaccct gcgtcacggt tacccgacca ccgaaaacgg tttctctctg accccgaacg
     1201 ttacccacgc tcgttctcgt ggtaccgttc gtctgcgttc tcgtgacttc cgtgacaaac
     1261 cgatggttga cccgcgttac ttcaccgacc cggaaggtca cgacatgcgt gttatggttg
     1321 ctggtatccg taaagctcgt gaaatcgctg ctcagccggc tatggctgaa tggaccggtc
     1381 gtgaactgtc tccgggtgtt gaagctcaga ccgacgaaga actgcaggac tacatccgta
     1441 aaacccacaa caccgtttac cacccggttg gtaccgttcg tatgggtgct gttgaagacg
     1501 aaatgtctcc gctggacccg gaactgcgtg ttaaaggtgt taccggtctg cgtgttgctg
     1561 acgcttctgt tatgccggaa cacgttaccg ttaacccgaa catcaccgtt atgatgatcg
     1621 gtgaacgttg cgctgacctg atccgttctg ctcgtgctgg tgaaaccacc accgctgacg
     1681 ctgaactgtc tgctgctctg gctactttat ctgagaatag tcaatcttcg gaaatcccag
     1741 gtggcatgca ggctaggtgg aggctcagtg gcgttctatc aataacccgt tggtgcaaag
     1801 cccgccgaaa ggcgggcttt tctgtccgtc tcagaatcgg ccgtgaacaa taaaatagtt
     1861 tcggtattat tgaccacttc cgagtagaat cgtgcttcag taagagtcga ccgatgccct
     1921 tgagagcctt caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg
     1981 cacttatgac tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcgctcttcc
     2041 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     2101 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     2161 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     2221 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     2281 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     2341 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     2401 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     2461 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     2521 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     2581 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     2641 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     2701 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     2761 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     2821 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     2881 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     2941 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     3001 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     3061 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     3121 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     3181 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     3241 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     3301 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     3361 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     3421 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     3481 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     3541 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat
     3601 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     3661 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     3721 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     3781 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     3841 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     3901 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     3961 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     4021 acgaggccct ttcgtcttca agaattctgg cgaatcctct gaccagccag aaaacgacct
     4081 ttctgtggtg aaaccggatg ctgcaattca gagcgccagc aagtggggga cagcagaaga
     4141 cctgaccgcc gcagagtgga tgtttgacat ggtgaagact atcgcaccat cagccagaaa
     4201 accgaatttt gctgggtggg ctaacgatat ccgcctgatg cgtgaacgtg acggacgtaa
     4261 ccaccgcgac atgtgtgtgc tgttccgctg gctcggatac ccttactctg ttgaaaacga
     4321 atagataggt taaggaacgg ttatttctgc gtagatctat cttacacagc atcacactgg
     4381 ctcaccttcg ggtgggcctt tctgcgttta tatactagag agagaatata aaaagccaga
     4441 ttattaatcc ggctttttta ttatttaggc aactgaaacg attcggatcc tgtattacta
     4501 ttc
//
