LOCUS       sequence-377972-       11864 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To act as a 1-input state of the split-NAND gate..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11864)
  AUTHORS   Lloyd JPB, Ly F, Gong P, Pflueger J, Swain T, Pflueger C, Fourie E, 
            Khan MA, Kidd BN, Lister R
  TITLE     Synthetic memory circuits for stable cell reprogramming in plants.
  JOURNAL   Nat Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2.
  PUBMED    35788565
REFERENCE   2  (bases 1 to 11864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2."
COMMENT     Sequence Label: LLP715_L2sV3_NAND_1IF1_FRT_Rluc_FlpF1_C1-NLS_lacZ
FEATURES             Location/Qualifiers
     source          1..11864
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    22..55
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    843..876
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     misc_feature    892..947
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     intron          979..1167
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     polyA_signal    2195..2369
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             2757..4409
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(2823..2841)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      4429..4629
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     misc_feature    4972..5027
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     intron          5712..5900
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     CDS             6775..6795
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6833..7007
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     protein_bind    7077..7098
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7113..7118,7119..7136,7137..7143)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7151..7167
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7156..7178
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7175..7191
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7175..7191
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             7187..7510
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    7204..7260
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(7261..7278)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7261..7277)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7270..7292)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(7486..7505)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    7642..7666
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      7754..8464
                     /label=oriV
                     /note="incP origin of replication"
     promoter        8485..8589
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8590..9384
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      9555..10143
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10044..10063
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             10479..11627
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    11773..11797
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgaatt cggatccgga ggaagttcct attctctaga aagtatagga acttctcgac
       61 aaaatttaga acgaacttaa ttatgatctc aaatacattg atacatatct catctagatc
      121 taggttatca ttatgtaaga aagttttgac gaatatggca cgacaaaatg gctagactcg
      181 atgtaattgg tatctcaact caacattata cttataccaa acattagtta gacaaaattt
      241 aaacaactat tttttatgta tgcaagagtc agcatatgta taattgattc agaatcgttt
      301 tgacgagttc ggatgtagta gtagccatta tttaatgtac atactaatcg tgaatagtga
      361 atatgatgaa acattgtatc ttattgtata aatatccata aacacatcat gaaagacact
      421 ttctttcacg gtctgaatta attatgatac aattctaata gaaaacgaat taaattacgt
      481 tgaattgtat gaaatctaat tgaacaagcc aaccacgacg acgactaacg ttgcctggat
      541 tgactcggtt taagttaacc actaaaaaaa cggagctgtc atgtaacacg cggatcgagc
      601 aggtcacagt catgaagcca tcaaagcaaa agaactaatc caagggctga gatgattaat
      661 tagtttaaaa attagttaac acgagggaaa aggctgtctg acagccaggt cacgttatct
      721 ttacctgtgg tcgaaatgat tcgtgtctgt cgattttaat tatttttttg aaaggccgaa
      781 aataaagttg taagagataa acccgcctat ataaattcat atattttcct ctccgctttg
      841 aagaagttcc tattctctag aaagtatagg aacttctact gtatttttac aacaattacc
      901 aacaacaaca aacaacaaac aacattacaa ttactattta caattacaat gacttcaaag
      961 gtgtatgatc ctgagcaggt aagtttctgc ttctaccttt gatatatata taataattat
     1021 cattaattag tagtaatata atatttcaaa tatttttttc aaaataaaag aatgtagtat
     1081 atagcaattg cttttctgta gtttataagt gtgtatattt taatttataa cttttctaat
     1141 atatgaccaa aatttgttga tgtgcagaga aaaagaatga taactggtcc acaatggtgg
     1201 gcaagatgta aacagatgaa cgtgcttgat tctttcatta attactacga ttcagaaaag
     1261 cacgctgaga atgcagttat ctttcttcac ggaaacgctg catcttcata tttgtggaga
     1321 catgttgtgc ctcacattga accagtggct aggtgtatta tcccagatct tatcggaatg
     1381 ggaaagtctg gtaaatctgg aaatggttct tacagacttt tggatcatta taagtacctc
     1441 acagcttggt tcgaacttct caacttacct aagaagatta ttttcgttgg acacgattgg
     1501 ggtgcttgct tggcattcca ttactcatac gaacaccaag ataagatcaa agctatagtt
     1561 catgcagaga gtgttgtgga tgtgatagaa tcttgggatg agtggccaga tattgaagag
     1621 gatattgctt tgatcaagtc tgaagaggga gaaaagatgg ttctcgaaaa taactttttc
     1681 gtggaaacta tgcttccttc aaagattatg agaaaattgg aaccagaaga gtttgctgca
     1741 tatctcgaac ctttcaagga aaaaggagag gttagaaggc caacactcag ttggcctagg
     1801 gaaataccat tagttaaggg aggtaaacct gatgttgtgc agatcgtgag aaattataac
     1861 gcttacctta gggcatcaga tgatttgcct aagatgttca tcgagtcaga tccaggattt
     1921 ttcagtaatg ctatagttga aggtgcaaag aaatttccaa acaccgagtt cgttaaggtg
     1981 aaaggattgc atttctcaca agaagatgct cctgatgaga tgggaaagta tattaagtct
     2041 tttgttgaaa gagtgctcaa aaacgagcag ggttctcgat ctgatgcttc tgaagtaacg
     2101 tcaacaagct cccagtctga ggtatgcaca gtggaaacgc caggttgtgt tggttcttaa
     2161 gcttctctag ctagagtcga tcgacaagct cgagtttctc cataataatg tgtgagtagt
     2221 tcccagataa gggaattagg gttcctatag ggtttcgctc atgtgttgag catataagaa
     2281 acccttagta tgtatttgta tttgtaaaat acttctatca ataaaatttc taattcctaa
     2341 aaccaaaatc cagtactaaa atccagatcg ctgcaagaat tcaagcttgg agccaacact
     2401 cgaatcccca cccgttggac caaacccggc tcattaagcg tcggttcaga tttatttcct
     2461 ttatttaaaa aaaaggaaag ggtaaaaaat agaaaattgg aaacagttaa agcccaaaat
     2521 tgtaatttac cgagaattgt aaatttacct gaaaacccta cgctatagtt tcgactataa
     2581 ataccaaact taggacctca cttcagaatc ccctcgtcgc tgcgtctctc tcccgcaacc
     2641 ttcgattttc gtttattcgc atccatcgga gagagaaaac aatcaataag cgacctacta
     2701 caaacaagta agtaagagaa aaaccaaaag aagaagagaa acaacaagaa gaagtaatgg
     2761 aggacgccaa aaacataaag aaaggcccgg cgccattcta tcctctagag gatggaaccg
     2821 ctggagagca actgcataag gctatgaaga gatacgccct ggttcctgga acaattgctt
     2881 ttacagatgc acatatcgag gtgaacatca cgtacgcgga atacttcgaa atgtccgttc
     2941 ggttggcaga agctatgaaa cgatatgggc tgaatacaaa tcacagaatc gtcgtatgca
     3001 gtgaaaactc tcttcaattc tttatgccgg tgttgggcgc gttatttatc ggagttgcag
     3061 ttgcgcccgc gaacgacatt tataatgaac gtgaattgct caacagtatg aacatttcgc
     3121 agcctaccgt agtgtttgtt tccaaaaagg ggttgcaaaa aattttgaac gtgcaaaaaa
     3181 aattaccaat aatccagaaa attattatca tggattctaa aacggattac cagggatttc
     3241 agtcgatgta cacgttcgtc acatctcatc tacctcccgg ttttaatgaa tacgattttg
     3301 taccagagtc ctttgatcgt gacaaaacaa ttgcactgat aatgaattcc tctggatcta
     3361 ctgggttacc taagggtgtg gcccttccgc atagaactgc ctgcgtcaga ttctcgcatg
     3421 ccagagatcc tatttttggc aatcaaatca ttccggatac tgcgatttta agtgttgttc
     3481 cattccatca cggttttgga atgtttacta cactcggata tttgatatgt ggatttcgag
     3541 tcgtcttaat gtatagattt gaagaagagc tgtttttacg atcccttcag gattacaaaa
     3601 ttcaaagtgc gttgctagta ccaaccctat tttcattctt cgccaaaagc actctgattg
     3661 acaaatacga tttatctaat ttacacgaaa ttgcttctgg gggcgcacct ctttcgaaag
     3721 aagtcgggga agcggttgca aaacgcttcc atcttccagg gatacgacaa ggatatgggc
     3781 tcactgagac tacatcagct attctgatta cacccgaggg ggatgataaa ccgggcgcgg
     3841 tcggtaaagt tgttccattt tttgaagcga aggttgtgga tctggatacc gggaaaacgc
     3901 tgggcgttaa tcagagaggc gaattatgtg tcagaggacc tatgattatg tccggttatg
     3961 taaacaatcc ggaagcgacc aacgccttga ttgacaagga tggatggcta cattctggag
     4021 acatagctta ctgggacgag gacgaacact tcttcatagt tgaccgcttg aagtctttaa
     4081 ttaaatacaa aggatatcag gtggcccccg ctgaattgga atcgatattg ttacaacacc
     4141 ccaacatctt cgacgcgggc gtggcaggtc tgcccgacga tgacgccggt gaacttcccg
     4201 ccgccgttgt tgttttggag cacggaaaga cgatgacgga aaaagagatc gtggattacg
     4261 tcgccagtca agtaacaacc gcgaaaaagt tgcgcggagg agttgtgttt gtggacgaag
     4321 taccgaaagg tcttaccgga aaactcgacg caagaaaaat cagagagatc ctcataaagg
     4381 ccaagaaggg cggaaagtcc aaattgtaag cttgatcccc cgtcgacagc tagctatatc
     4441 atcaatttat gtattacaca taatatcgca ctcagtcttt catctacggc aatgtaccag
     4501 ctgatataat cagttattga aatatttctg aatttaaact tgcatcaata aatttatgtt
     4561 tttgcttgga ctataatacc tgacttgtta ttttatcaat aaatatttaa actatatttc
     4621 tttcaagatc gctactagaa ttcgagctcg gagccaacac tcgaatcccc acccgttgga
     4681 ccaaacccgg ctcattaagc gtcggttcag atttatttcc tttatttaaa aaaaaggaaa
     4741 gggtaaaaaa tagaaaattg gaaacagtta aagcccaaaa ttgtaattta ccgagaattg
     4801 taaatttacc tgaaaaccct acgctatagt ttcgactata aataccaaac ttaggacctc
     4861 acttcagaat cccctcgtcg ctgcgtctct ctcccgcaac cttcgatttt cgtttattcg
     4921 catccatcgg agagagaaaa caatcaataa gcgacctact gtatttttac aacaattacc
     4981 aacaacaaca aacaacaaac aacattacaa ttactattta caattacaat gagccagttc
     5041 gacatcctgt gcaagacccc ccccaaggtg ctggtgcggc agttcgtgga gagattcgag
     5101 aggcccagcg gcgagaagat cgccagctgt gccgccgagc tgacctacct gtgctggatg
     5161 atcacccaca acggcaccgc catcaagagg gccaccttca tgagctacaa caccatcatc
     5221 agcaacagcc tgagcttcga catcgtgaac aagagcctgc agttcaagta caagacccag
     5281 aaggccacca tcctggaggc cagcctgaag aagctgatcc ccgcctggga gttcaccatc
     5341 atcccttaca acggccagaa gcaccagagc gacatcaccg acatcgtgtc cagcctgcag
     5401 ctgcagttcg agagcagcga ggaggccgac aagggcaaca gccacagcaa gaagatgctg
     5461 aaggccctgc tgtccgaggg cgagagcatc tgggagatca ccgagaagat cctgaacagc
     5521 ttcgagtaca ccagcaggtt caccaagacc aagaccctgt accagttcct gttcctggcc
     5581 acattcatca actgcggcag gttcagcgac atcaagaacg tggaccccaa gagcttcaag
     5641 ctggtgcaga acaagtacct gggcgtgatc attcagtgcc tggtgaccga gactaagaca
     5701 agcgtgtcca ggtaagtttc tgcttctacc tttgatatat atataataat tatcattaat
     5761 tagtagtaat ataatatttc aaatattttt ttcaaaataa aagaatgtag tatatagcaa
     5821 ttgcttttct gtagtttata agtgtgtata ttttaattta taacttttct aatatatgac
     5881 caaaatttgt tgatgtgcag gcacatctac tttttcagcg ccagaggcag gatcgacccc
     5941 ctggtgtacc tggacgagtt cctgaggaac agcgagcccg tgctgaagag agtgaacagg
     6001 accggcaaca gcagcagcaa caagcaggag taccagctgc tgaaggacaa cctggtgcgc
     6061 agctacaaca aggccctgaa gaagaacgcc ccctacccca tcttcgctat caagaacggc
     6121 cctaagagcc acatcggcag gcacctgatg accagctttc tgagcatgaa gggcctgacc
     6181 gagctgacaa acgtggtggg caactggagc gacaagaggg cctccgccgt ggccaggacc
     6241 acctacaccc accagatcac cgccatcccc gaccactact tcgccctggt gtccaggtac
     6301 tacgcctacg accccatcag caaggagatg atcgccctga aggacgagac taaccccatc
     6361 gaggagtggc agcacatcga gcagctgaag ggcagcgccg agggcagcct ctccggaggg
     6421 tctggctccg gatcaagtgg tggcagcggt acttcgagca caaccaaaaa agccagtgat
     6481 tctgcattct ggcttgaggt tgaaggtaat tccatgaccg caccaacagg ctccaagcca
     6541 agctttcctg acggaatgtt aattctcgtt gaccctgagc aggctgttga gccaggtgat
     6601 ttctgcatag ccagacttgg gggtgatgag tttaccttca agaaactgat cagggatagc
     6661 ggtcaggtgt ttttacaacc actaaaccca cagtacccaa tgatcccatg caatgagagt
     6721 tgttccgttg tggggaaagt tatcgctagt cagtggcctg aagagacgtt tggccccaag
     6781 aaaaagcgga aggtgtaggc ttctctagct agagtcgatc gacaagctcg agtttctcca
     6841 taataatgtg tgagtagttc ccagataagg gaattagggt tcctataggg tttcgctcat
     6901 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     6961 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatcgct ttactgagac
     7021 cgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     7081 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     7141 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
     7201 gccaagcttg catgcctgca ggtcgactct agaggatccc cgggtaccga gctcgaattc
     7261 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     7321 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     7381 cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt attttctcct
     7441 tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga
     7501 tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     7561 ttgtctgctc ccggcatccg cttacagaca agctgtgacg gtctcaggga cacgaagtga
     7621 tccgtttaaa ctatcagtgt ttgacaggat atattggcgg gtaaacctaa gagaaaagag
     7681 cgtttattag aataatcgga tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt
     7741 tgtatgtgcc agccgccttt gcgacgctca ccgggctggt tgccctcgcc gctgggctgg
     7801 cggccgtcta tggccctgca aacgcgccag aaacgccgtc gaagccgtgt gcgagacacc
     7861 gcggccgccg gcgttgtgga tacctcgcgg aaaacttggc cctcactgac agatgagggg
     7921 cggacgttga cacttgaggg gccgactcac ccggcgcggc gttgacagat gaggggcagg
     7981 ctcgatttcg gccggcgacg tggagctggc cagcctcgca aatcggcgaa aacgcctgat
     8041 tttacgcgag tttcccacag atgatgtgga caagcctggg gataagtgcc ctgcggtatt
     8101 gacacttgag gggcgcgact actgacagat gaggggcgcg atccttgaca cttgaggggc
     8161 agagtgctga cagatgaggg gcgcacctat tgacatttga ggggctgtcc acaggcagaa
     8221 aatccagcat ttgcaagggt ttccgcccgt ttttcggcca ccgctaacct gtcttttaac
     8281 ctgcttttaa accaatattt ataaaccttg tttttaacca gggctgcgcc ctgtgcgcgt
     8341 gaccgcgcac gccgaagggg ggtgcccccc cttctcgaac cctcccggcc cgctaacgcg
     8401 ggcctcccat ccccccaggg gctgcgcccc tcggccgcga acggcctcac cccaaaaatg
     8461 gcagcgctgg ccaattcccg agtgcgcgga acccctattt gtttattttt ctaaatacat
     8521 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     8581 aggaagagta tggctaaaat gagaatatca ccggaattga aaaaactgat cgaaaaatac
     8641 cgctgcgtaa aagatacgga aggaatgtct cctgctaagg tatataagct ggtgggagaa
     8701 aatgaaaacc tatatttaaa aatgacggac agccggtata aagggaccac ctatgatgtg
     8761 gaacgggaaa aggacatgat gctatggctg gaaggaaagc tgcctgttcc aaaggtcctg
     8821 cactttgaac ggcatgatgg ctggagcaat ctgctcatga gtgaggccga tggcgtcctt
     8881 tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga ttatcgagct gtatgcggag
     8941 tgcatcaggc tctttcactc catcgacata tcggattgtc cctatacgaa tagcttagac
     9001 agccgcttag ccgaattgga ttacttactg aataacgatc tggccgatgt ggattgcgaa
     9061 aactgggaag aggacactcc atttaaagat ccgcgcgagc tgtatgattt tttaaagacg
     9121 gaaaagcccg aagaggaact tgtcttttcc cacggcgacc tgggagacag caacatcttt
     9181 gtgaaagatg gcaaagtaag tggctttatt gatcttggga gaagcggcag ggcggacaag
     9241 tggtatgaca ttgccttctg cgtccggtcg ctcagggagg atatcgggga agaacagtat
     9301 gtcgagctat tttttgactt actggggatc aagcctgatt gggagaaaat aaaatattat
     9361 attttactgg atgaattgtt ttagctgtca gaccaagttt actcatatat actttagatt
     9421 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     9481 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     9541 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     9601 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     9661 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     9721 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     9781 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     9841 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     9901 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     9961 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
    10021 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
    10081 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
    10141 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcag atcctagatg tggcgcaacg
    10201 atgccggcga caagcaggag cgcaccgact tcttccgcat caagtgtttt ggctctcagg
    10261 ccgaggccca cggcaagtat ttgggcaagg ggtcgctggt attcgtgcag ggcaagattc
    10321 ggaataccaa gtacgagaag gacggccaga cggtctacgg gaccgacttc attgccgata
    10381 aggtggatta tctggacacc aaggcaccag gcgggtcaaa tcaggaataa gggcacattg
    10441 ccccggcgtg agtcggggca atcccgcaag gagggtgaat gaatcggacg tttgaccgga
    10501 aggcatacag gcaagaactg atcgacgcgg ggttttccgc cgaggatgcc gaaaccatcg
    10561 caagccgcac cgtcatgcgt gcgccccgcg aaaccttcca gtccgtcggc tcgatggtcc
    10621 agcaagctac ggccaagatc gagcgcgaca gcgtgcaact ggctccccct gccctgcccg
    10681 cgccatcggc cgccgtggag cgttcgcgtc gtcttgaaca ggaggcggca ggtttggcga
    10741 agtcgatgac catcgacacg cgaggaacta tgacgaccaa gaagcgaaaa accgccggcg
    10801 aggacctggc aaaacaggtc agcgaggcca agcaggccgc gttgctgaaa cacacgaagc
    10861 agcagatcaa ggaaatgcag ctttccttgt tcgatattgc gccgtggccg gacacgatgc
    10921 gagcgatgcc aaacgacacg gcccgctctg ccctgttcac cacgcgcaac aagaaaatcc
    10981 cgcgcgaggc gctgcaaaac aaggtcattt tccacgtcaa caaggacgtg aagatcacct
    11041 acaccggcgt cgagctgcgg gccgacgatg acgaactggt gtggcagcag gtgttggagt
    11101 acgcgaagcg cacccctatc ggcgagccga tcaccttcac gttctacgag ctttgccagg
    11161 acctgggctg gtcgatcaat ggccggtatt acacgaaggc cgaggaatgc ctgtcgcgcc
    11221 tacaggcgac ggcgatgggc ttcacgtccg accgcgttgg gcacctggaa tcggtgtcgc
    11281 tgctgcaccg cttccgcgtc ctggaccgtg gcaagaaaac gtcccgttgc caggtcctga
    11341 tcgacgagga aatcgtcgtg ctgtttgctg gcgaccacta cacgaaattc atatgggaga
    11401 agtaccgcaa gctgtcgccg acggcccgac ggatgttcga ctatttcagc tcgcaccggg
    11461 agccgtaccc gctcaagctg gaaaccttcc gcctcatgtg cggatcggat tccacccgcg
    11521 tgaagaagtg gcgcgagcag gtcggcgaag cctgcgaaga gttgcgaggc agcggcctgg
    11581 tggaacacgc ctgggtcaat gatgacctgg tgcattgcaa acgctagggc cttgtggggt
    11641 cagttccggc tgggggttca gcagcccctg ctcggatctg ttggaccgga cagtagtcat
    11701 ggttgatggg ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg
    11761 ttggctggct ggtggcagga tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa
    11821 taacacattg cggacgtttt taatgtactg gggttgaaca ctct
//
