LOCUS       sequence-377973-       12948 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To act as the 2-input state of the split-NAND gate..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12948)
  AUTHORS   Lloyd JPB, Ly F, Gong P, Pflueger J, Swain T, Pflueger C, Fourie E, 
            Khan MA, Kidd BN, Lister R
  TITLE     Synthetic memory circuits for stable cell reprogramming in plants.
  JOURNAL   Nat Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2.
  PUBMED    35788565
REFERENCE   2  (bases 1 to 12948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2."
COMMENT     Sequence Label: LLP717_L2sV3_NAND_2I_FRT_Rluc_Flp-C1_lacZ
FEATURES             Location/Qualifiers
     source          1..12948
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    22..55
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    843..876
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     misc_feature    892..947
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     intron          979..1167
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     polyA_signal    2195..2369
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             2757..4409
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(2823..2841)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      4429..4629
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     misc_feature    4972..5027
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     intron          5712..5900
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     CDS             6775..6795
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6833..7007
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    7349..7404
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             7859..7879
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7917..8091
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     protein_bind    8161..8182
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8197..8202,8203..8220,8221..8227)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8235..8251
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8240..8262
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8259..8275
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8259..8275
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             8271..8594
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    8288..8344
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(8345..8362)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8345..8361)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8354..8376)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8570..8589)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    8726..8750
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      8838..9548
                     /label=oriV
                     /note="incP origin of replication"
     promoter        9569..9673
                     /gene="bla"
                     /label=AmpR promoter
     CDS             9674..10468
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      10639..11227
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11128..11147
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             11563..12711
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    12857..12881
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgaatt cggatccgga ggaagttcct attctctaga aagtatagga acttctcgac
       61 aaaatttaga acgaacttaa ttatgatctc aaatacattg atacatatct catctagatc
      121 taggttatca ttatgtaaga aagttttgac gaatatggca cgacaaaatg gctagactcg
      181 atgtaattgg tatctcaact caacattata cttataccaa acattagtta gacaaaattt
      241 aaacaactat tttttatgta tgcaagagtc agcatatgta taattgattc agaatcgttt
      301 tgacgagttc ggatgtagta gtagccatta tttaatgtac atactaatcg tgaatagtga
      361 atatgatgaa acattgtatc ttattgtata aatatccata aacacatcat gaaagacact
      421 ttctttcacg gtctgaatta attatgatac aattctaata gaaaacgaat taaattacgt
      481 tgaattgtat gaaatctaat tgaacaagcc aaccacgacg acgactaacg ttgcctggat
      541 tgactcggtt taagttaacc actaaaaaaa cggagctgtc atgtaacacg cggatcgagc
      601 aggtcacagt catgaagcca tcaaagcaaa agaactaatc caagggctga gatgattaat
      661 tagtttaaaa attagttaac acgagggaaa aggctgtctg acagccaggt cacgttatct
      721 ttacctgtgg tcgaaatgat tcgtgtctgt cgattttaat tatttttttg aaaggccgaa
      781 aataaagttg taagagataa acccgcctat ataaattcat atattttcct ctccgctttg
      841 aagaagttcc tattctctag aaagtatagg aacttctact gtatttttac aacaattacc
      901 aacaacaaca aacaacaaac aacattacaa ttactattta caattacaat gacttcaaag
      961 gtgtatgatc ctgagcaggt aagtttctgc ttctaccttt gatatatata taataattat
     1021 cattaattag tagtaatata atatttcaaa tatttttttc aaaataaaag aatgtagtat
     1081 atagcaattg cttttctgta gtttataagt gtgtatattt taatttataa cttttctaat
     1141 atatgaccaa aatttgttga tgtgcagaga aaaagaatga taactggtcc acaatggtgg
     1201 gcaagatgta aacagatgaa cgtgcttgat tctttcatta attactacga ttcagaaaag
     1261 cacgctgaga atgcagttat ctttcttcac ggaaacgctg catcttcata tttgtggaga
     1321 catgttgtgc ctcacattga accagtggct aggtgtatta tcccagatct tatcggaatg
     1381 ggaaagtctg gtaaatctgg aaatggttct tacagacttt tggatcatta taagtacctc
     1441 acagcttggt tcgaacttct caacttacct aagaagatta ttttcgttgg acacgattgg
     1501 ggtgcttgct tggcattcca ttactcatac gaacaccaag ataagatcaa agctatagtt
     1561 catgcagaga gtgttgtgga tgtgatagaa tcttgggatg agtggccaga tattgaagag
     1621 gatattgctt tgatcaagtc tgaagaggga gaaaagatgg ttctcgaaaa taactttttc
     1681 gtggaaacta tgcttccttc aaagattatg agaaaattgg aaccagaaga gtttgctgca
     1741 tatctcgaac ctttcaagga aaaaggagag gttagaaggc caacactcag ttggcctagg
     1801 gaaataccat tagttaaggg aggtaaacct gatgttgtgc agatcgtgag aaattataac
     1861 gcttacctta gggcatcaga tgatttgcct aagatgttca tcgagtcaga tccaggattt
     1921 ttcagtaatg ctatagttga aggtgcaaag aaatttccaa acaccgagtt cgttaaggtg
     1981 aaaggattgc atttctcaca agaagatgct cctgatgaga tgggaaagta tattaagtct
     2041 tttgttgaaa gagtgctcaa aaacgagcag ggttctcgat ctgatgcttc tgaagtaacg
     2101 tcaacaagct cccagtctga ggtatgcaca gtggaaacgc caggttgtgt tggttcttaa
     2161 gcttctctag ctagagtcga tcgacaagct cgagtttctc cataataatg tgtgagtagt
     2221 tcccagataa gggaattagg gttcctatag ggtttcgctc atgtgttgag catataagaa
     2281 acccttagta tgtatttgta tttgtaaaat acttctatca ataaaatttc taattcctaa
     2341 aaccaaaatc cagtactaaa atccagatcg ctgcaagaat tcaagcttgg agccaacact
     2401 cgaatcccca cccgttggac caaacccggc tcattaagcg tcggttcaga tttatttcct
     2461 ttatttaaaa aaaaggaaag ggtaaaaaat agaaaattgg aaacagttaa agcccaaaat
     2521 tgtaatttac cgagaattgt aaatttacct gaaaacccta cgctatagtt tcgactataa
     2581 ataccaaact taggacctca cttcagaatc ccctcgtcgc tgcgtctctc tcccgcaacc
     2641 ttcgattttc gtttattcgc atccatcgga gagagaaaac aatcaataag cgacctacta
     2701 caaacaagta agtaagagaa aaaccaaaag aagaagagaa acaacaagaa gaagtaatgg
     2761 aggacgccaa aaacataaag aaaggcccgg cgccattcta tcctctagag gatggaaccg
     2821 ctggagagca actgcataag gctatgaaga gatacgccct ggttcctgga acaattgctt
     2881 ttacagatgc acatatcgag gtgaacatca cgtacgcgga atacttcgaa atgtccgttc
     2941 ggttggcaga agctatgaaa cgatatgggc tgaatacaaa tcacagaatc gtcgtatgca
     3001 gtgaaaactc tcttcaattc tttatgccgg tgttgggcgc gttatttatc ggagttgcag
     3061 ttgcgcccgc gaacgacatt tataatgaac gtgaattgct caacagtatg aacatttcgc
     3121 agcctaccgt agtgtttgtt tccaaaaagg ggttgcaaaa aattttgaac gtgcaaaaaa
     3181 aattaccaat aatccagaaa attattatca tggattctaa aacggattac cagggatttc
     3241 agtcgatgta cacgttcgtc acatctcatc tacctcccgg ttttaatgaa tacgattttg
     3301 taccagagtc ctttgatcgt gacaaaacaa ttgcactgat aatgaattcc tctggatcta
     3361 ctgggttacc taagggtgtg gcccttccgc atagaactgc ctgcgtcaga ttctcgcatg
     3421 ccagagatcc tatttttggc aatcaaatca ttccggatac tgcgatttta agtgttgttc
     3481 cattccatca cggttttgga atgtttacta cactcggata tttgatatgt ggatttcgag
     3541 tcgtcttaat gtatagattt gaagaagagc tgtttttacg atcccttcag gattacaaaa
     3601 ttcaaagtgc gttgctagta ccaaccctat tttcattctt cgccaaaagc actctgattg
     3661 acaaatacga tttatctaat ttacacgaaa ttgcttctgg gggcgcacct ctttcgaaag
     3721 aagtcgggga agcggttgca aaacgcttcc atcttccagg gatacgacaa ggatatgggc
     3781 tcactgagac tacatcagct attctgatta cacccgaggg ggatgataaa ccgggcgcgg
     3841 tcggtaaagt tgttccattt tttgaagcga aggttgtgga tctggatacc gggaaaacgc
     3901 tgggcgttaa tcagagaggc gaattatgtg tcagaggacc tatgattatg tccggttatg
     3961 taaacaatcc ggaagcgacc aacgccttga ttgacaagga tggatggcta cattctggag
     4021 acatagctta ctgggacgag gacgaacact tcttcatagt tgaccgcttg aagtctttaa
     4081 ttaaatacaa aggatatcag gtggcccccg ctgaattgga atcgatattg ttacaacacc
     4141 ccaacatctt cgacgcgggc gtggcaggtc tgcccgacga tgacgccggt gaacttcccg
     4201 ccgccgttgt tgttttggag cacggaaaga cgatgacgga aaaagagatc gtggattacg
     4261 tcgccagtca agtaacaacc gcgaaaaagt tgcgcggagg agttgtgttt gtggacgaag
     4321 taccgaaagg tcttaccgga aaactcgacg caagaaaaat cagagagatc ctcataaagg
     4381 ccaagaaggg cggaaagtcc aaattgtaag cttgatcccc cgtcgacagc tagctatatc
     4441 atcaatttat gtattacaca taatatcgca ctcagtcttt catctacggc aatgtaccag
     4501 ctgatataat cagttattga aatatttctg aatttaaact tgcatcaata aatttatgtt
     4561 tttgcttgga ctataatacc tgacttgtta ttttatcaat aaatatttaa actatatttc
     4621 tttcaagatc gctactagaa ttcgagctcg gagccaacac tcgaatcccc acccgttgga
     4681 ccaaacccgg ctcattaagc gtcggttcag atttatttcc tttatttaaa aaaaaggaaa
     4741 gggtaaaaaa tagaaaattg gaaacagtta aagcccaaaa ttgtaattta ccgagaattg
     4801 taaatttacc tgaaaaccct acgctatagt ttcgactata aataccaaac ttaggacctc
     4861 acttcagaat cccctcgtcg ctgcgtctct ctcccgcaac cttcgatttt cgtttattcg
     4921 catccatcgg agagagaaaa caatcaataa gcgacctact gtatttttac aacaattacc
     4981 aacaacaaca aacaacaaac aacattacaa ttactattta caattacaat gagccagttc
     5041 gacatcctgt gcaagacccc ccccaaggtg ctggtgcggc agttcgtgga gagattcgag
     5101 aggcccagcg gcgagaagat cgccagctgt gccgccgagc tgacctacct gtgctggatg
     5161 atcacccaca acggcaccgc catcaagagg gccaccttca tgagctacaa caccatcatc
     5221 agcaacagcc tgagcttcga catcgtgaac aagagcctgc agttcaagta caagacccag
     5281 aaggccacca tcctggaggc cagcctgaag aagctgatcc ccgcctggga gttcaccatc
     5341 atcccttaca acggccagaa gcaccagagc gacatcaccg acatcgtgtc cagcctgcag
     5401 ctgcagttcg agagcagcga ggaggccgac aagggcaaca gccacagcaa gaagatgctg
     5461 aaggccctgc tgtccgaggg cgagagcatc tgggagatca ccgagaagat cctgaacagc
     5521 ttcgagtaca ccagcaggtt caccaagacc aagaccctgt accagttcct gttcctggcc
     5581 acattcatca actgcggcag gttcagcgac atcaagaacg tggaccccaa gagcttcaag
     5641 ctggtgcaga acaagtacct gggcgtgatc attcagtgcc tggtgaccga gactaagaca
     5701 agcgtgtcca ggtaagtttc tgcttctacc tttgatatat atataataat tatcattaat
     5761 tagtagtaat ataatatttc aaatattttt ttcaaaataa aagaatgtag tatatagcaa
     5821 ttgcttttct gtagtttata agtgtgtata ttttaattta taacttttct aatatatgac
     5881 caaaatttgt tgatgtgcag gcacatctac tttttcagcg ccagaggcag gatcgacccc
     5941 ctggtgtacc tggacgagtt cctgaggaac agcgagcccg tgctgaagag agtgaacagg
     6001 accggcaaca gcagcagcaa caagcaggag taccagctgc tgaaggacaa cctggtgcgc
     6061 agctacaaca aggccctgaa gaagaacgcc ccctacccca tcttcgctat caagaacggc
     6121 cctaagagcc acatcggcag gcacctgatg accagctttc tgagcatgaa gggcctgacc
     6181 gagctgacaa acgtggtggg caactggagc gacaagaggg cctccgccgt ggccaggacc
     6241 acctacaccc accagatcac cgccatcccc gaccactact tcgccctggt gtccaggtac
     6301 tacgcctacg accccatcag caaggagatg atcgccctga aggacgagac taaccccatc
     6361 gaggagtggc agcacatcga gcagctgaag ggcagcgccg agggcagcct ctccggaggg
     6421 tctggctccg gatcaagtgg tggcagcggt acttcgagca caaccaaaaa agccagtgat
     6481 tctgcattct ggcttgaggt tgaaggtaat tccatgaccg caccaacagg ctccaagcca
     6541 agctttcctg acggaatgtt aattctcgtt gaccctgagc aggctgttga gccaggtgat
     6601 ttctgcatag ccagacttgg gggtgatgag tttaccttca agaaactgat cagggatagc
     6661 ggtcaggtgt ttttacaacc actaaaccca cagtacccaa tgatcccatg caatgagagt
     6721 tgttccgttg tggggaaagt tatcgctagt cagtggcctg aagagacgtt tggccccaag
     6781 aaaaagcgga aggtgtaggc ttctctagct agagtcgatc gacaagctcg agtttctcca
     6841 taataatgtg tgagtagttc ccagataagg gaattagggt tcctataggg tttcgctcat
     6901 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     6961 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatcgct ttacgaattc
     7021 ccatggggag ccaacactcg aatccccacc cgttggacca aacccggctc attaagcgtc
     7081 ggttcagatt tatttccttt atttaaaaaa aaggaaaggg taaaaaatag aaaattggaa
     7141 acagttaaag cccaaaattg taatttaccg agaattgtaa atttacctga aaaccctacg
     7201 ctatagtttc gactataaat accaaactta ggacctcact tcagaatccc ctcgtcgctg
     7261 cgtctctctc ccgcaacctt cgattttcgt ttattcgcat ccatcggaga gagaaaacaa
     7321 tcaataagcg acctactgta tttttacaac aattaccaac aacaacaaac aacaaacaac
     7381 attacaatta ctatttacaa ttacaatgag cacaaccaaa aaagccagtg attctgcatt
     7441 ctggcttgag gttgaaggta attccatgac cgcaccaaca ggctccaagc caagctttcc
     7501 tgacggaatg ttaattctcg ttgaccctga gcaggctgtt gagccaggtg atttctgcat
     7561 agccagactt gggggtgatg agtttacctt caagaaactg atcagggata gcggtcaggt
     7621 gtttttacaa ccactaaacc cacagtaccc aatgatccca tgcaatgaga gttgttccgt
     7681 tgtggggaaa gttatcgcta gtcagtggcc tgaagagacg tttggcagcc tctccggagg
     7741 gtctggctcc ggatcaagtg gtggcagcgg tacttcgagc atcagatacc ccgcctggaa
     7801 cggcatcatc agccaggagg tgctggacta cctgagcagc tacatcaaca ggcggatccc
     7861 caagaaaaag cggaaggtgt gagcttctct agctagagtc gatcgacaag ctcgagtttc
     7921 tccataataa tgtgtgagta gttcccagat aagggaatta gggttcctat agggtttcgc
     7981 tcatgtgttg agcatataag aaacccttag tatgtatttg tatttgtaaa atacttctat
     8041 caataaaatt tctaattcct aaaaccaaaa tccagtacta aaatccagat cgctcagatg
     8101 agaccgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     8161 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     8221 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
     8281 ttacgccaag cttgcatgcc tgcaggtcga ctctagagga tccccgggta ccgagctcga
     8341 attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     8401 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     8461 atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc
     8521 tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct
     8581 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
     8641 gggcttgtct gctcccggca tccgcttaca gacaagctgt gacggtctca gggacacgaa
     8701 gtgatccgtt taaactatca gtgtttgaca ggatatattg gcgggtaaac ctaagagaaa
     8761 agagcgttta ttagaataat cggatattta aaagggcgtg aaaaggttta tccgttcgtc
     8821 catttgtatg tgccagccgc ctttgcgacg ctcaccgggc tggttgccct cgccgctggg
     8881 ctggcggccg tctatggccc tgcaaacgcg ccagaaacgc cgtcgaagcc gtgtgcgaga
     8941 caccgcggcc gccggcgttg tggatacctc gcggaaaact tggccctcac tgacagatga
     9001 ggggcggacg ttgacacttg aggggccgac tcacccggcg cggcgttgac agatgagggg
     9061 caggctcgat ttcggccggc gacgtggagc tggccagcct cgcaaatcgg cgaaaacgcc
     9121 tgattttacg cgagtttccc acagatgatg tggacaagcc tggggataag tgccctgcgg
     9181 tattgacact tgaggggcgc gactactgac agatgagggg cgcgatcctt gacacttgag
     9241 gggcagagtg ctgacagatg aggggcgcac ctattgacat ttgaggggct gtccacaggc
     9301 agaaaatcca gcatttgcaa gggtttccgc ccgtttttcg gccaccgcta acctgtcttt
     9361 taacctgctt ttaaaccaat atttataaac cttgttttta accagggctg cgccctgtgc
     9421 gcgtgaccgc gcacgccgaa ggggggtgcc cccccttctc gaaccctccc ggcccgctaa
     9481 cgcgggcctc ccatcccccc aggggctgcg cccctcggcc gcgaacggcc tcaccccaaa
     9541 aatggcagcg ctggccaatt cccgagtgcg cggaacccct atttgtttat ttttctaaat
     9601 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     9661 aaaaaggaag agtatggcta aaatgagaat atcaccggaa ttgaaaaaac tgatcgaaaa
     9721 ataccgctgc gtaaaagata cggaaggaat gtctcctgct aaggtatata agctggtggg
     9781 agaaaatgaa aacctatatt taaaaatgac ggacagccgg tataaaggga ccacctatga
     9841 tgtggaacgg gaaaaggaca tgatgctatg gctggaagga aagctgcctg ttccaaaggt
     9901 cctgcacttt gaacggcatg atggctggag caatctgctc atgagtgagg ccgatggcgt
     9961 cctttgctcg gaagagtatg aagatgaaca aagccctgaa aagattatcg agctgtatgc
    10021 ggagtgcatc aggctctttc actccatcga catatcggat tgtccctata cgaatagctt
    10081 agacagccgc ttagccgaat tggattactt actgaataac gatctggccg atgtggattg
    10141 cgaaaactgg gaagaggaca ctccatttaa agatccgcgc gagctgtatg attttttaaa
    10201 gacggaaaag cccgaagagg aacttgtctt ttcccacggc gacctgggag acagcaacat
    10261 ctttgtgaaa gatggcaaag taagtggctt tattgatctt gggagaagcg gcagggcgga
    10321 caagtggtat gacattgcct tctgcgtccg gtcgctcagg gaggatatcg gggaagaaca
    10381 gtatgtcgag ctattttttg acttactggg gatcaagcct gattgggaga aaataaaata
    10441 ttatatttta ctggatgaat tgttttagct gtcagaccaa gtttactcat atatacttta
    10501 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
    10561 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
    10621 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
    10681 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
    10741 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
    10801 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
    10861 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
    10921 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
    10981 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
    11041 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
    11101 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
    11161 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
    11221 atggaaaaac gccagcaacg cggccttttt acggttcctg gcagatccta gatgtggcgc
    11281 aacgatgccg gcgacaagca ggagcgcacc gacttcttcc gcatcaagtg ttttggctct
    11341 caggccgagg cccacggcaa gtatttgggc aaggggtcgc tggtattcgt gcagggcaag
    11401 attcggaata ccaagtacga gaaggacggc cagacggtct acgggaccga cttcattgcc
    11461 gataaggtgg attatctgga caccaaggca ccaggcgggt caaatcagga ataagggcac
    11521 attgccccgg cgtgagtcgg ggcaatcccg caaggagggt gaatgaatcg gacgtttgac
    11581 cggaaggcat acaggcaaga actgatcgac gcggggtttt ccgccgagga tgccgaaacc
    11641 atcgcaagcc gcaccgtcat gcgtgcgccc cgcgaaacct tccagtccgt cggctcgatg
    11701 gtccagcaag ctacggccaa gatcgagcgc gacagcgtgc aactggctcc ccctgccctg
    11761 cccgcgccat cggccgccgt ggagcgttcg cgtcgtcttg aacaggaggc ggcaggtttg
    11821 gcgaagtcga tgaccatcga cacgcgagga actatgacga ccaagaagcg aaaaaccgcc
    11881 ggcgaggacc tggcaaaaca ggtcagcgag gccaagcagg ccgcgttgct gaaacacacg
    11941 aagcagcaga tcaaggaaat gcagctttcc ttgttcgata ttgcgccgtg gccggacacg
    12001 atgcgagcga tgccaaacga cacggcccgc tctgccctgt tcaccacgcg caacaagaaa
    12061 atcccgcgcg aggcgctgca aaacaaggtc attttccacg tcaacaagga cgtgaagatc
    12121 acctacaccg gcgtcgagct gcgggccgac gatgacgaac tggtgtggca gcaggtgttg
    12181 gagtacgcga agcgcacccc tatcggcgag ccgatcacct tcacgttcta cgagctttgc
    12241 caggacctgg gctggtcgat caatggccgg tattacacga aggccgagga atgcctgtcg
    12301 cgcctacagg cgacggcgat gggcttcacg tccgaccgcg ttgggcacct ggaatcggtg
    12361 tcgctgctgc accgcttccg cgtcctggac cgtggcaaga aaacgtcccg ttgccaggtc
    12421 ctgatcgacg aggaaatcgt cgtgctgttt gctggcgacc actacacgaa attcatatgg
    12481 gagaagtacc gcaagctgtc gccgacggcc cgacggatgt tcgactattt cagctcgcac
    12541 cgggagccgt acccgctcaa gctggaaacc ttccgcctca tgtgcggatc ggattccacc
    12601 cgcgtgaaga agtggcgcga gcaggtcggc gaagcctgcg aagagttgcg aggcagcggc
    12661 ctggtggaac acgcctgggt caatgatgac ctggtgcatt gcaaacgcta gggccttgtg
    12721 gggtcagttc cggctggggg ttcagcagcc cctgctcgga tctgttggac cggacagtag
    12781 tcatggttga tgggctgcct gtatcgagtg gtgattttgt gccgagctgc cggtcgggga
    12841 gctgttggct ggctggtggc aggatatatt gtggtgtaaa caaattgacg cttagacaac
    12901 ttaataacac attgcggacg tttttaatgt actggggttg aacactct
//
