LOCUS       sequence-377976-       12716 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To act as the on state for testing of the alt B3 recombinase..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12716)
  AUTHORS   Lloyd JPB, Ly F, Gong P, Pflueger J, Swain T, Pflueger C, Fourie E, 
            Khan MA, Kidd BN, Lister R
  TITLE     Synthetic memory circuits for stable cell reprogramming in plants.
  JOURNAL   Nat Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2.
  PUBMED    35788565
REFERENCE   2  (bases 1 to 12716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2."
COMMENT     Sequence Label: LLP740_L2pV1_1I_B3RT-OCS-Rluc_TCTP-B3o_lacZ
FEATURES             Location/Qualifiers
     source          1..12716
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      877..1584
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     intron          1684..1872
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     polyA_signal    2900..3074
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             3462..5114
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(3528..3546)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      5134..5334
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     misc_feature    5677..5732
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             5737..5757
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          6535..6723
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     polyA_signal    7685..7859
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     protein_bind    7929..7950
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7965..7970,7971..7988,7989..7995)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8003..8019
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8008..8030
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8027..8043
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8027..8043
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             8039..8362
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    8056..8112
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(8113..8130)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8113..8129)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8122..8144)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8338..8357)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    8494..8518
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      8606..9316
                     /label=oriV
                     /note="incP origin of replication"
     promoter        9337..9441
                     /gene="bla"
                     /label=AmpR promoter
     CDS             9442..10236
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      10407..10995
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10896..10915
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             11331..12479
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    12625..12649
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccaattc ggatccggag tcgacaaaat ttagaacgaa cttaattatg atctcaaata
       61 cattgataca tatctcatct agatctaggt tatcattatg taagaaagtt ttgacgaata
      121 tggcacgaca aaatggctag actcgatgta attggtatct caactcaaca ttatacttat
      181 accaaacatt agttagacaa aatttaaaca actatttttt atgtatgcaa gagtcagcat
      241 atgtataatt gattcagaat cgttttgacg agttcggatg tagtagtagc cattatttaa
      301 tgtacatact aatcgtgaat agtgaatatg atgaaacatt gtatcttatt gtataaatat
      361 ccataaacac atcatgaaag acactttctt tcacggtctg aattaattat gatacaattc
      421 taatagaaaa cgaattaaat tacgttgaat tgtatgaaat ctaattgaac aagccaacca
      481 cgacgacgac taacgttgcc tggattgact cggtttaagt taaccactaa aaaaacggag
      541 ctgtcatgta acacgcggat cgagcaggtc acagtcatga agccatcaaa gcaaaagaac
      601 taatccaagg gctgagatga ttaattagtt taaaaattag ttaacacgag ggaaaaggct
      661 gtctgacagc caggtcacgt tatctttacc tgtggtcgaa atgattcgtg tctgtcgatt
      721 ttaattattt ttttgaaagg ccgaaaataa agttgtaaga gataaacccg cctatataaa
      781 ttcatatatt ttcctctccg ctttgaatac tgtattttta caacaattac caacaacaac
      841 aaaggttgct taagaataag taattcttaa gcaaccctgc tttaatgaga tatgcgagaa
      901 gcctatgatc gcatgatatt tgctttcaat tctgttgtgc acgttgtaaa aaacctgagc
      961 atgtgtagct cagatcctta ccgccggttt cggttcattc taatgaatat atcacccgtt
     1021 actatcgtat ttttatgaat aatattctcc gttcaattta ctgattgtac cctactactt
     1081 atatgtacaa tattaaaatg aaaacaatat attgtgctga ataggtttat agcgacatct
     1141 atgatagagc gccacaataa caaacaattg cgttttatta ttacaaatcc aattttaaaa
     1201 aaagcggcag aaccggtcaa acctaaaaga ctgattacat aaatcttatt caaatttcaa
     1261 aagtgcccca ggggctagta tctacgacac accgagcggc gaactaataa cgctcactga
     1321 agggaactcc ggttccccgc cggcgcgcat gggtgagatt ccttgaagtt gagtattggc
     1381 cgtccgctct accgaaagtt acgggcacca ttcaacccgg tccagcacgg cggccgggta
     1441 accgacttgc tgccccgaga attatgcagc atttttttgg tgtatgtggg ccccaaatga
     1501 agtgcaggtc aaaccttgac agtgacgaca aatcgttggg cgggtccagg gcgaattttg
     1561 cgacaacatg tcgaggctca gcagggttgc ttaagaataa gtaattctta agcaacccaa
     1621 caaacaacat tacaattact atttacaatt acaatgactt caaaggtgta tgatcctgag
     1681 caggtaagtt tctgcttcta cctttgatat atatataata attatcatta attagtagta
     1741 atataatatt tcaaatattt ttttcaaaat aaaagaatgt agtatatagc aattgctttt
     1801 ctgtagttta taagtgtgta tattttaatt tataactttt ctaatatatg accaaaattt
     1861 gttgatgtgc agagaaaaag aatgataact ggtccacaat ggtgggcaag atgtaaacag
     1921 atgaacgtgc ttgattcttt cattaattac tacgattcag aaaagcacgc tgagaatgca
     1981 gttatctttc ttcacggaaa cgctgcatct tcatatttgt ggagacatgt tgtgcctcac
     2041 attgaaccag tggctaggtg tattatccca gatcttatcg gaatgggaaa gtctggtaaa
     2101 tctggaaatg gttcttacag acttttggat cattataagt acctcacagc ttggttcgaa
     2161 cttctcaact tacctaagaa gattattttc gttggacacg attggggtgc ttgcttggca
     2221 ttccattact catacgaaca ccaagataag atcaaagcta tagttcatgc agagagtgtt
     2281 gtggatgtga tagaatcttg ggatgagtgg ccagatattg aagaggatat tgctttgatc
     2341 aagtctgaag agggagaaaa gatggttctc gaaaataact ttttcgtgga aactatgctt
     2401 ccttcaaaga ttatgagaaa attggaacca gaagagtttg ctgcatatct cgaacctttc
     2461 aaggaaaaag gagaggttag aaggccaaca ctcagttggc ctagggaaat accattagtt
     2521 aagggaggta aacctgatgt tgtgcagatc gtgagaaatt ataacgctta ccttagggca
     2581 tcagatgatt tgcctaagat gttcatcgag tcagatccag gatttttcag taatgctata
     2641 gttgaaggtg caaagaaatt tccaaacacc gagttcgtta aggtgaaagg attgcatttc
     2701 tcacaagaag atgctcctga tgagatggga aagtatatta agtcttttgt tgaaagagtg
     2761 ctcaaaaacg agcagggttc tcgatctgat gcttctgaag taacgtcaac aagctcccag
     2821 tctgaggtat gcacagtgga aacgccaggt tgtgttggtt cttaagcttc tctagctaga
     2881 gtcgatcgac aagctcgagt ttctccataa taatgtgtga gtagttccca gataagggaa
     2941 ttagggttcc tatagggttt cgctcatgtg ttgagcatat aagaaaccct tagtatgtat
     3001 ttgtatttgt aaaatacttc tatcaataaa atttctaatt cctaaaacca aaatccagta
     3061 ctaaaatcca gatcgctgca agaattcaag cttggagcca acactcgaat ccccacccgt
     3121 tggaccaaac ccggctcatt aagcgtcggt tcagatttat ttcctttatt taaaaaaaag
     3181 gaaagggtaa aaaatagaaa attggaaaca gttaaagccc aaaattgtaa tttaccgaga
     3241 attgtaaatt tacctgaaaa ccctacgcta tagtttcgac tataaatacc aaacttagga
     3301 cctcacttca gaatcccctc gtcgctgcgt ctctctcccg caaccttcga ttttcgttta
     3361 ttcgcatcca tcggagagag aaaacaatca ataagcgacc tactacaaac aagtaagtaa
     3421 gagaaaaacc aaaagaagaa gagaaacaac aagaagaagt aatggaggac gccaaaaaca
     3481 taaagaaagg cccggcgcca ttctatcctc tagaggatgg aaccgctgga gagcaactgc
     3541 ataaggctat gaagagatac gccctggttc ctggaacaat tgcttttaca gatgcacata
     3601 tcgaggtgaa catcacgtac gcggaatact tcgaaatgtc cgttcggttg gcagaagcta
     3661 tgaaacgata tgggctgaat acaaatcaca gaatcgtcgt atgcagtgaa aactctcttc
     3721 aattctttat gccggtgttg ggcgcgttat ttatcggagt tgcagttgcg cccgcgaacg
     3781 acatttataa tgaacgtgaa ttgctcaaca gtatgaacat ttcgcagcct accgtagtgt
     3841 ttgtttccaa aaaggggttg caaaaaattt tgaacgtgca aaaaaaatta ccaataatcc
     3901 agaaaattat tatcatggat tctaaaacgg attaccaggg atttcagtcg atgtacacgt
     3961 tcgtcacatc tcatctacct cccggtttta atgaatacga ttttgtacca gagtcctttg
     4021 atcgtgacaa aacaattgca ctgataatga attcctctgg atctactggg ttacctaagg
     4081 gtgtggccct tccgcataga actgcctgcg tcagattctc gcatgccaga gatcctattt
     4141 ttggcaatca aatcattccg gatactgcga ttttaagtgt tgttccattc catcacggtt
     4201 ttggaatgtt tactacactc ggatatttga tatgtggatt tcgagtcgtc ttaatgtata
     4261 gatttgaaga agagctgttt ttacgatccc ttcaggatta caaaattcaa agtgcgttgc
     4321 tagtaccaac cctattttca ttcttcgcca aaagcactct gattgacaaa tacgatttat
     4381 ctaatttaca cgaaattgct tctgggggcg cacctctttc gaaagaagtc ggggaagcgg
     4441 ttgcaaaacg cttccatctt ccagggatac gacaaggata tgggctcact gagactacat
     4501 cagctattct gattacaccc gagggggatg ataaaccggg cgcggtcggt aaagttgttc
     4561 cattttttga agcgaaggtt gtggatctgg ataccgggaa aacgctgggc gttaatcaga
     4621 gaggcgaatt atgtgtcaga ggacctatga ttatgtccgg ttatgtaaac aatccggaag
     4681 cgaccaacgc cttgattgac aaggatggat ggctacattc tggagacata gcttactggg
     4741 acgaggacga acacttcttc atagttgacc gcttgaagtc tttaattaaa tacaaaggat
     4801 atcaggtggc ccccgctgaa ttggaatcga tattgttaca acaccccaac atcttcgacg
     4861 cgggcgtggc aggtctgccc gacgatgacg ccggtgaact tcccgccgcc gttgttgttt
     4921 tggagcacgg aaagacgatg acggaaaaag agatcgtgga ttacgtcgcc agtcaagtaa
     4981 caaccgcgaa aaagttgcgc ggaggagttg tgtttgtgga cgaagtaccg aaaggtctta
     5041 ccggaaaact cgacgcaaga aaaatcagag agatcctcat aaaggccaag aagggcggaa
     5101 agtccaaatt gtaagcttga tcccccgtcg acagctagct atatcatcaa tttatgtatt
     5161 acacataata tcgcactcag tctttcatct acggcaatgt accagctgat ataatcagtt
     5221 attgaaatat ttctgaattt aaacttgcat caataaattt atgtttttgc ttggactata
     5281 atacctgact tgttatttta tcaataaata tttaaactat atttctttca agatcgctac
     5341 tagaattcga gctcggagcc aacactcgaa tccccacccg ttggaccaaa cccggctcat
     5401 taagcgtcgg ttcagattta tttcctttat ttaaaaaaaa ggaaagggta aaaaatagaa
     5461 aattggaaac agttaaagcc caaaattgta atttaccgag aattgtaaat ttacctgaaa
     5521 accctacgct atagtttcga ctataaatac caaacttagg acctcacttc agaatcccct
     5581 cgtcgctgcg tctctctccc gcaaccttcg attttcgttt attcgcatcc atcggagaga
     5641 gaaaacaatc aataagcgac ctactgtatt tttacaacaa ttaccaacaa caacaaacaa
     5701 caaacaacat tacaattact atttacaatt acaatgccca agaaaaagcg gaaggtgtcc
     5761 agctacatgg acctggtgga cgacgagccc gccaccctgt accacaagtt cgtggaatgc
     5821 ctgaaggccg gcgagaactt ctgcggcgat aagctgagcg gcatcatcac catggccatt
     5881 ctgaaggcca tcaaggccct gaccgaagtg aagaaaacca ccttcaacaa gtacaagacc
     5941 accatcaagc agggcctgca gtacgacgtg ggcagcagca ccatcagctt cgtgtaccac
     6001 ctgaaggact gcgacgagct gagcagaggc ctgagcgacg ccttcgagcc ctacaagttc
     6061 aagatcaaga gcaacaaaga ggccaccagc ttcaagaccc tgttcagggg ccctagcttc
     6121 ggcagccaga agaactggcg gaagaaagag gtggaccgcg aggtggacaa cctgttccac
     6181 agcaccgaga cagacgagag catcttcaag ttcatcctga acaccctgga cagcatcgaa
     6241 acccagacca acaccgaccg gcagaaaacc gtgctgacct ttatcctgct gatgaccttc
     6301 ttcaactgct gccggaacaa cgacctgatg aacgtggacc ccagcacctt caagatcgtg
     6361 aagaacaagt ttgtgggcta cctgctgcag gctgaagtga agcagaccaa gaccagaaag
     6421 agccggaata tcttcttctt ccccatccgg gaaaaccgct tcgacctgtt cctggccctg
     6481 cacgacttct tcagaacctg ccagcccacc cccaagagca gactgagcga tcaggtaagt
     6541 ttctgcttct acctttgata tatatataat aattatcatt aattagtagt aatataatat
     6601 ttcaaatatt tttttcaaaa taaaagaatg tagtatatag caattgcttt tctgtagttt
     6661 ataagtgtgt atattttaat ttataacttt tctaatatat gaccaaaatt tgttgatgtg
     6721 caggtgtccg agcagaagtg gcagctgttc cgggacagca tggtcatcga ctacaaccgg
     6781 ttctttcgga agttccccgc cagccccatc ttcgccatta agcacggccc caagtcccac
     6841 ctgggccggc atctgatgaa cagctttctg cacaagaacg agctggacag ctgggccaac
     6901 agcctgggca attggagcag ctcccagaac cagagagaga gcggcgccag actgggctac
     6961 acacacggcg gaagagatct gccccagccc ctgtttggct tcctggccgg atactgcgtg
     7021 cggaacgaag agggccacat cgtgggcctg ggcctggaaa aggacatcaa cgatctgttc
     7081 gacggcatca tggaccccct gaacgagaaa gaggacaccg agatctgcga gagctacggc
     7141 gagtgggcca agattgtgtc caaggacgtg ctgatcttcc tgaagagata ccacagcaag
     7201 aacgcctgtc ggagatacca gaacagcacc ctgtatgccc ggaccttcct gaaaaccgag
     7261 agcgtgaccc tgagcggctc caagggcagc gaggaacctt ctagccctgt gcggatcccc
     7321 atcctgagca tgggaaaggc cagcccctcc gagggaagaa agctgagagc cagcgagcac
     7381 gccaacgacg acaacgagat cgagaagatc gacagcgaca gcagccagag cgaagagatc
     7441 cctatcgaga tgagcgactc cgaggacgag acaaccgcca gcaacatcag cggcatctac
     7501 ctggacatga gcaaggccaa ctccaacgtg gtgtacagcc cccctagcca gacaggcaga
     7561 gctgctggcg ccggaagaaa aagaggcgtg ggaggcagac ggaccgtgga aagcaagcgg
     7621 agaagagtgc tggcccccat caaccggtga gcttctctag ctagagtcga tcgacaagct
     7681 cgagtttctc cataataatg tgtgagtagt tcccagataa gggaattagg gttcctatag
     7741 ggtttcgctc atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat
     7801 acttctatca ataaaatttc taattcctaa aaccaaaatc cagtactaaa atccagatcg
     7861 ctttactgag accgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     7921 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     7981 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
     8041 gaccatgatt acgccaagct tgcatgcctg caggtcgact ctagaggatc cccgggtacc
     8101 gagctcgaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     8161 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     8221 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg
     8281 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac
     8341 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc
     8401 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga cggtctcagg
     8461 gacacgaagt gatccgttta aactatcagt gtttgacagg atatattggc gggtaaacct
     8521 aagagaaaag agcgtttatt agaataatcg gatatttaaa agggcgtgaa aaggtttatc
     8581 cgttcgtcca tttgtatgtg ccagccgcct ttgcgacgct caccgggctg gttgccctcg
     8641 ccgctgggct ggcggccgtc tatggccctg caaacgcgcc agaaacgccg tcgaagccgt
     8701 gtgcgagaca ccgcggccgc cggcgttgtg gatacctcgc ggaaaacttg gccctcactg
     8761 acagatgagg ggcggacgtt gacacttgag gggccgactc acccggcgcg gcgttgacag
     8821 atgaggggca ggctcgattt cggccggcga cgtggagctg gccagcctcg caaatcggcg
     8881 aaaacgcctg attttacgcg agtttcccac agatgatgtg gacaagcctg gggataagtg
     8941 ccctgcggta ttgacacttg aggggcgcga ctactgacag atgaggggcg cgatccttga
     9001 cacttgaggg gcagagtgct gacagatgag gggcgcacct attgacattt gaggggctgt
     9061 ccacaggcag aaaatccagc atttgcaagg gtttccgccc gtttttcggc caccgctaac
     9121 ctgtctttta acctgctttt aaaccaatat ttataaacct tgtttttaac cagggctgcg
     9181 ccctgtgcgc gtgaccgcgc acgccgaagg ggggtgcccc cccttctcga accctcccgg
     9241 cccgctaacg cgggcctccc atccccccag gggctgcgcc cctcggccgc gaacggcctc
     9301 accccaaaaa tggcagcgct ggccaattcc cgagtgcgcg gaacccctat ttgtttattt
     9361 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     9421 taatattgaa aaaggaagag tatggctaaa atgagaatat caccggaatt gaaaaaactg
     9481 atcgaaaaat accgctgcgt aaaagatacg gaaggaatgt ctcctgctaa ggtatataag
     9541 ctggtgggag aaaatgaaaa cctatattta aaaatgacgg acagccggta taaagggacc
     9601 acctatgatg tggaacggga aaaggacatg atgctatggc tggaaggaaa gctgcctgtt
     9661 ccaaaggtcc tgcactttga acggcatgat ggctggagca atctgctcat gagtgaggcc
     9721 gatggcgtcc tttgctcgga agagtatgaa gatgaacaaa gccctgaaaa gattatcgag
     9781 ctgtatgcgg agtgcatcag gctctttcac tccatcgaca tatcggattg tccctatacg
     9841 aatagcttag acagccgctt agccgaattg gattacttac tgaataacga tctggccgat
     9901 gtggattgcg aaaactggga agaggacact ccatttaaag atccgcgcga gctgtatgat
     9961 tttttaaaga cggaaaagcc cgaagaggaa cttgtctttt cccacggcga cctgggagac
    10021 agcaacatct ttgtgaaaga tggcaaagta agtggcttta ttgatcttgg gagaagcggc
    10081 agggcggaca agtggtatga cattgccttc tgcgtccggt cgctcaggga ggatatcggg
    10141 gaagaacagt atgtcgagct attttttgac ttactgggga tcaagcctga ttgggagaaa
    10201 ataaaatatt atattttact ggatgaattg ttttagctgt cagaccaagt ttactcatat
    10261 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
    10321 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
    10381 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
    10441 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
    10501 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
    10561 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
    10621 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
    10681 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
    10741 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
    10801 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
    10861 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
    10921 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
    10981 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc agatcctaga
    11041 tgtggcgcaa cgatgccggc gacaagcagg agcgcaccga cttcttccgc atcaagtgtt
    11101 ttggctctca ggccgaggcc cacggcaagt atttgggcaa ggggtcgctg gtattcgtgc
    11161 agggcaagat tcggaatacc aagtacgaga aggacggcca gacggtctac gggaccgact
    11221 tcattgccga taaggtggat tatctggaca ccaaggcacc aggcgggtca aatcaggaat
    11281 aagggcacat tgccccggcg tgagtcgggg caatcccgca aggagggtga atgaatcgga
    11341 cgtttgaccg gaaggcatac aggcaagaac tgatcgacgc ggggttttcc gccgaggatg
    11401 ccgaaaccat cgcaagccgc accgtcatgc gtgcgccccg cgaaaccttc cagtccgtcg
    11461 gctcgatggt ccagcaagct acggccaaga tcgagcgcga cagcgtgcaa ctggctcccc
    11521 ctgccctgcc cgcgccatcg gccgccgtgg agcgttcgcg tcgtcttgaa caggaggcgg
    11581 caggtttggc gaagtcgatg accatcgaca cgcgaggaac tatgacgacc aagaagcgaa
    11641 aaaccgccgg cgaggacctg gcaaaacagg tcagcgaggc caagcaggcc gcgttgctga
    11701 aacacacgaa gcagcagatc aaggaaatgc agctttcctt gttcgatatt gcgccgtggc
    11761 cggacacgat gcgagcgatg ccaaacgaca cggcccgctc tgccctgttc accacgcgca
    11821 acaagaaaat cccgcgcgag gcgctgcaaa acaaggtcat tttccacgtc aacaaggacg
    11881 tgaagatcac ctacaccggc gtcgagctgc gggccgacga tgacgaactg gtgtggcagc
    11941 aggtgttgga gtacgcgaag cgcaccccta tcggcgagcc gatcaccttc acgttctacg
    12001 agctttgcca ggacctgggc tggtcgatca atggccggta ttacacgaag gccgaggaat
    12061 gcctgtcgcg cctacaggcg acggcgatgg gcttcacgtc cgaccgcgtt gggcacctgg
    12121 aatcggtgtc gctgctgcac cgcttccgcg tcctggaccg tggcaagaaa acgtcccgtt
    12181 gccaggtcct gatcgacgag gaaatcgtcg tgctgtttgc tggcgaccac tacacgaaat
    12241 tcatatggga gaagtaccgc aagctgtcgc cgacggcccg acggatgttc gactatttca
    12301 gctcgcaccg ggagccgtac ccgctcaagc tggaaacctt ccgcctcatg tgcggatcgg
    12361 attccacccg cgtgaagaag tggcgcgagc aggtcggcga agcctgcgaa gagttgcgag
    12421 gcagcggcct ggtggaacac gcctgggtca atgatgacct ggtgcattgc aaacgctagg
    12481 gccttgtggg gtcagttccg gctgggggtt cagcagcccc tgctcggatc tgttggaccg
    12541 gacagtagtc atggttgatg ggctgcctgt atcgagtggt gattttgtgc cgagctgccg
    12601 gtcggggagc tgttggctgg ctggtggcag gatatattgt ggtgtaaaca aattgacgct
    12661 tagacaactt aataacacat tgcggacgtt tttaatgtac tggggttgaa cactct
//
