LOCUS       sequence-377977-        3924 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express the bacterial lactate oxidase enzyme under the control of 
            the strong constitutif phage promoter T7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3924)
  AUTHORS   Soudier P, Zuniga A, Duigou T, Voyvodic PL, Bazi-Kabbaj K, Kushwaha 
            M, Vendrell JA, Solassol J, Bonnet J, Faulon JL
  TITLE     PeroxiHUB: A Modular Cell-Free Biosensing Platform Using H(2)O(2) as
            Signal Integrator.
  JOURNAL   ACS Synth Biol. 2022 Aug 19;11(8):2578-2588. doi: 
            10.1021/acssynbio.2c00138. Epub 2022 Aug 1.
  PUBMED    35913043
REFERENCE   2  (bases 1 to 3924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.2c00138"; journalName: "ACS Synth Biol"; date: 
            "2022-08-19- 19"; volume: "11"; issue: "8"; pages: "2578-2588"
COMMENT     Sequence Label: pBeast-pT7-lox
FEATURES             Location/Qualifiers
     source          1..3924
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     9..28
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9..27
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             44..66
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(1472..1489)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1643..2231)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1723..1742)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2402..3193,3194..3262))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3025..3044
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3263..3367)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3435..3453
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      3805..3832
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 tattcttata atacgactca ctatagggag agctagcaat aattttgttt aactttaaga
       61 aggagatata atgaataaca atgacattga atataatgca cctagtgaaa tcaagtacat
      121 tgatgttgtc aatacttacg acttagaaga agaagcaagt aaagtggtac cacatggtgg
      181 ttttaactat attgccggtg catctggtga tgagtggact aaacgcgcta atgaccgtgc
      241 ttggaaacat aaattactat acccacgtct agcgcaagat gttgaagcgc ccgatacaag
      301 tactgaaatt ttaggtcata aaattaaagc cccattcatc atggcaccaa ttgctgcaca
      361 tggtttagcc cacactacta aagaagctgg tactgcacgt gcagtttcag aatttggtac
      421 aattatgtcc atctcagctt attctggtgc aacatttgaa gaaatttctg aaggcttaaa
      481 tggcggaccc cgttggttcc aaatctatat ggctaaagat gaccaacaaa accgtgatat
      541 cttagacgaa gctaaatctg atggtgcaac tgctatcatc cttacagctg actcaactgt
      601 ttctggaaac cgtgaccgtg atgtgaagaa taaattcgtt tacccatttg gtatgccaat
      661 tgttcaacgt tacttacgtg gtacagcaga aggtatgtca ttaaacaata tctacggtgc
      721 ttcaaaacaa aaaatctcac caagagatat tgaggaaatc gccgctcatt ctggattacc
      781 agtattcgtt aaaggtattc aacacccaga agatgcagat atggcaatca aagctggtgc
      841 atcaggtatc tgggtatcta accacggtgc tcgtcaacta tatgaagctc caggttcatt
      901 tgacaccctt ccagctattg ctgaacgtgt aaacaaacgt gtaccaatcg tctttgattc
      961 aggtgtacgt cgtggtgaac acgttgccaa agcgctagct tcaggggcag acgttgttgc
     1021 tttaggacgc ccagtcttat ttggtttagc tttaggtggc tggcaaggtg cttactcagt
     1081 acttgactac ttccaaaaag acttaacacg cgtaatgcaa ttaacaggtt cacaaaatgt
     1141 ggaagacttg aagggtctag atttattcga taacccatac ggttatgaat actagagcgt
     1201 tctatcaata acccgttggt gcaaagcccg ccgaaaggcg ggcttttctg tccgtctcag
     1261 aatcggccgt gaacaataaa atagtttcgg tattattgac cacttccgag tagaatcgtg
     1321 cttcagtaag agtcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg
     1381 ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt
     1441 aggacaggtg ccggcagcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     1501 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     1561 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     1621 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     1681 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     1741 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     1801 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     1861 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     1921 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     1981 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     2041 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     2101 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     2161 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     2221 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     2281 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     2341 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     2401 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     2461 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     2521 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     2581 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     2641 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     2701 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     2761 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     2821 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     2881 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     2941 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     3001 gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     3061 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     3121 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     3181 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     3241 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     3301 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     3361 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     3421 cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttcaagaa ttctggcgaa
     3481 tcctctgacc agccagaaaa cgacctttct gtggtgaaac cggatgctgc aattcagagc
     3541 gccagcaagt gggggacagc agaagacctg accgccgcag agtggatgtt tgacatggtg
     3601 aagactatcg caccatcagc cagaaaaccg aattttgctg ggtgggctaa cgatatccgc
     3661 ctgatgcgtg aacgtgacgg acgtaaccac cgcgacatgt gtgtgctgtt ccgctggctc
     3721 ggataccctt actctgttga aaacgaatag ataggttaag gaacggttat ttctgcgtag
     3781 atctatctta cacagcatca cactggctca ccttcgggtg ggcctttctg cgtttatata
     3841 ctagagagag aatataaaaa gccagattat taatccggct tttttattat ttaggcaact
     3901 gaaacgattc ggatcctgta ttac
//
