LOCUS       sequence-377979-       12413 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To test if the 35S promoter can drive the Flp recomhinase in order 
            to remove the OCS terminator and increase circuit output..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12413)
  AUTHORS   Lloyd JPB, Ly F, Gong P, Pflueger J, Swain T, Pflueger C, Fourie E, 
            Khan MA, Kidd BN, Lister R
  TITLE     Synthetic memory circuits for stable cell reprogramming in plants.
  JOURNAL   Nat Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2.
  PUBMED    35788565
REFERENCE   2  (bases 1 to 12413)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2."
COMMENT     Sequence Label: LLP738_L2pV1_1I_Act2_FRT_OCS_s35S_Flp_lacZ
FEATURES             Location/Qualifiers
     source          1..12413
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    845..878
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     terminator      879..1586
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     protein_bind    1587..1620
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     intron          1687..1875
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     polyA_signal    2903..3077
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             3465..5117
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(3531..3549)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      5137..5337
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     promoter        5439..5784
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     5727..5747
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    5803..5858
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             5866..5886
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          6570..6758
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     polyA_signal    7382..7556
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     protein_bind    7626..7647
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7662..7667,7668..7685,7686..7692)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7700..7716
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7705..7727
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7724..7740
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7724..7740
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             7736..8059
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    7753..7809
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(7810..7827)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7810..7826)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7819..7841)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8035..8054)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    8191..8215
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      8303..9013
                     /label=oriV
                     /note="incP origin of replication"
     promoter        9034..9138
                     /gene="bla"
                     /label=AmpR promoter
     CDS             9139..9933
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      10104..10692
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10593..10612
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             11028..12176
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    12322..12346
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgaatt cggatccgga gtcgacaaaa tttagaacga acttaattat gatctcaaat
       61 acattgatac atatctcatc tagatctagg ttatcattat gtaagaaagt tttgacgaat
      121 atggcacgac aaaatggcta gactcgatgt aattggtatc tcaactcaac attatactta
      181 taccaaacat tagttagaca aaatttaaac aactattttt tatgtatgca agagtcagca
      241 tatgtataat tgattcagaa tcgttttgac gagttcggat gtagtagtag ccattattta
      301 atgtacatac taatcgtgaa tagtgaatat gatgaaacat tgtatcttat tgtataaata
      361 tccataaaca catcatgaaa gacactttct ttcacggtct gaattaatta tgatacaatt
      421 ctaatagaaa acgaattaaa ttacgttgaa ttgtatgaaa tctaattgaa caagccaacc
      481 acgacgacga ctaacgttgc ctggattgac tcggtttaag ttaaccacta aaaaaacgga
      541 gctgtcatgt aacacgcgga tcgagcaggt cacagtcatg aagccatcaa agcaaaagaa
      601 ctaatccaag ggctgagatg attaattagt ttaaaaatta gttaacacga gggaaaaggc
      661 tgtctgacag ccaggtcacg ttatctttac ctgtggtcga aatgattcgt gtctgtcgat
      721 tttaattatt tttttgaaag gccgaaaata aagttgtaag agataaaccc gcctatataa
      781 attcatatat tttcctctcc gctttgaata ctgtattttt acaacaatta ccaacaacaa
      841 caaagaagtt cctattctct agaaagtata ggaacttcct gctttaatga gatatgcgag
      901 aagcctatga tcgcatgata tttgctttca attctgttgt gcacgttgta aaaaacctga
      961 gcatgtgtag ctcagatcct taccgccggt ttcggttcat tctaatgaat atatcacccg
     1021 ttactatcgt atttttatga ataatattct ccgttcaatt tactgattgt accctactac
     1081 ttatatgtac aatattaaaa tgaaaacaat atattgtgct gaataggttt atagcgacat
     1141 ctatgataga gcgccacaat aacaaacaat tgcgttttat tattacaaat ccaattttaa
     1201 aaaaagcggc agaaccggtc aaacctaaaa gactgattac ataaatctta ttcaaatttc
     1261 aaaagtgccc caggggctag tatctacgac acaccgagcg gcgaactaat aacgctcact
     1321 gaagggaact ccggttcccc gccggcgcgc atgggtgaga ttccttgaag ttgagtattg
     1381 gccgtccgct ctaccgaaag ttacgggcac cattcaaccc ggtccagcac ggcggccggg
     1441 taaccgactt gctgccccga gaattatgca gcattttttt ggtgtatgtg ggccccaaat
     1501 gaagtgcagg tcaaaccttg acagtgacga caaatcgttg ggcgggtcca gggcgaattt
     1561 tgcgacaaca tgtcgaggct cagcaggaag ttcctattct ctagaaagta taggaacttc
     1621 caacaaacaa cattacaatt actatttaca attacaatga cttcaaaggt gtatgatcct
     1681 gagcaggtaa gtttctgctt ctacctttga tatatatata ataattatca ttaattagta
     1741 gtaatataat atttcaaata tttttttcaa aataaaagaa tgtagtatat agcaattgct
     1801 tttctgtagt ttataagtgt gtatatttta atttataact tttctaatat atgaccaaaa
     1861 tttgttgatg tgcagagaaa aagaatgata actggtccac aatggtgggc aagatgtaaa
     1921 cagatgaacg tgcttgattc tttcattaat tactacgatt cagaaaagca cgctgagaat
     1981 gcagttatct ttcttcacgg aaacgctgca tcttcatatt tgtggagaca tgttgtgcct
     2041 cacattgaac cagtggctag gtgtattatc ccagatctta tcggaatggg aaagtctggt
     2101 aaatctggaa atggttctta cagacttttg gatcattata agtacctcac agcttggttc
     2161 gaacttctca acttacctaa gaagattatt ttcgttggac acgattgggg tgcttgcttg
     2221 gcattccatt actcatacga acaccaagat aagatcaaag ctatagttca tgcagagagt
     2281 gttgtggatg tgatagaatc ttgggatgag tggccagata ttgaagagga tattgctttg
     2341 atcaagtctg aagagggaga aaagatggtt ctcgaaaata actttttcgt ggaaactatg
     2401 cttccttcaa agattatgag aaaattggaa ccagaagagt ttgctgcata tctcgaacct
     2461 ttcaaggaaa aaggagaggt tagaaggcca acactcagtt ggcctaggga aataccatta
     2521 gttaagggag gtaaacctga tgttgtgcag atcgtgagaa attataacgc ttaccttagg
     2581 gcatcagatg atttgcctaa gatgttcatc gagtcagatc caggattttt cagtaatgct
     2641 atagttgaag gtgcaaagaa atttccaaac accgagttcg ttaaggtgaa aggattgcat
     2701 ttctcacaag aagatgctcc tgatgagatg ggaaagtata ttaagtcttt tgttgaaaga
     2761 gtgctcaaaa acgagcaggg ttctcgatct gatgcttctg aagtaacgtc aacaagctcc
     2821 cagtctgagg tatgcacagt ggaaacgcca ggttgtgttg gttcttaagc ttctctagct
     2881 agagtcgatc gacaagctcg agtttctcca taataatgtg tgagtagttc ccagataagg
     2941 gaattagggt tcctataggg tttcgctcat gtgttgagca tataagaaac ccttagtatg
     3001 tatttgtatt tgtaaaatac ttctatcaat aaaatttcta attcctaaaa ccaaaatcca
     3061 gtactaaaat ccagatcgct gcaagaattc aagcttggag ccaacactcg aatccccacc
     3121 cgttggacca aacccggctc attaagcgtc ggttcagatt tatttccttt atttaaaaaa
     3181 aaggaaaggg taaaaaatag aaaattggaa acagttaaag cccaaaattg taatttaccg
     3241 agaattgtaa atttacctga aaaccctacg ctatagtttc gactataaat accaaactta
     3301 ggacctcact tcagaatccc ctcgtcgctg cgtctctctc ccgcaacctt cgattttcgt
     3361 ttattcgcat ccatcggaga gagaaaacaa tcaataagcg acctactaca aacaagtaag
     3421 taagagaaaa accaaaagaa gaagagaaac aacaagaaga agtaatggag gacgccaaaa
     3481 acataaagaa aggcccggcg ccattctatc ctctagagga tggaaccgct ggagagcaac
     3541 tgcataaggc tatgaagaga tacgccctgg ttcctggaac aattgctttt acagatgcac
     3601 atatcgaggt gaacatcacg tacgcggaat acttcgaaat gtccgttcgg ttggcagaag
     3661 ctatgaaacg atatgggctg aatacaaatc acagaatcgt cgtatgcagt gaaaactctc
     3721 ttcaattctt tatgccggtg ttgggcgcgt tatttatcgg agttgcagtt gcgcccgcga
     3781 acgacattta taatgaacgt gaattgctca acagtatgaa catttcgcag cctaccgtag
     3841 tgtttgtttc caaaaagggg ttgcaaaaaa ttttgaacgt gcaaaaaaaa ttaccaataa
     3901 tccagaaaat tattatcatg gattctaaaa cggattacca gggatttcag tcgatgtaca
     3961 cgttcgtcac atctcatcta cctcccggtt ttaatgaata cgattttgta ccagagtcct
     4021 ttgatcgtga caaaacaatt gcactgataa tgaattcctc tggatctact gggttaccta
     4081 agggtgtggc ccttccgcat agaactgcct gcgtcagatt ctcgcatgcc agagatccta
     4141 tttttggcaa tcaaatcatt ccggatactg cgattttaag tgttgttcca ttccatcacg
     4201 gttttggaat gtttactaca ctcggatatt tgatatgtgg atttcgagtc gtcttaatgt
     4261 atagatttga agaagagctg tttttacgat cccttcagga ttacaaaatt caaagtgcgt
     4321 tgctagtacc aaccctattt tcattcttcg ccaaaagcac tctgattgac aaatacgatt
     4381 tatctaattt acacgaaatt gcttctgggg gcgcacctct ttcgaaagaa gtcggggaag
     4441 cggttgcaaa acgcttccat cttccaggga tacgacaagg atatgggctc actgagacta
     4501 catcagctat tctgattaca cccgaggggg atgataaacc gggcgcggtc ggtaaagttg
     4561 ttccattttt tgaagcgaag gttgtggatc tggataccgg gaaaacgctg ggcgttaatc
     4621 agagaggcga attatgtgtc agaggaccta tgattatgtc cggttatgta aacaatccgg
     4681 aagcgaccaa cgccttgatt gacaaggatg gatggctaca ttctggagac atagcttact
     4741 gggacgagga cgaacacttc ttcatagttg accgcttgaa gtctttaatt aaatacaaag
     4801 gatatcaggt ggcccccgct gaattggaat cgatattgtt acaacacccc aacatcttcg
     4861 acgcgggcgt ggcaggtctg cccgacgatg acgccggtga acttcccgcc gccgttgttg
     4921 ttttggagca cggaaagacg atgacggaaa aagagatcgt ggattacgtc gccagtcaag
     4981 taacaaccgc gaaaaagttg cgcggaggag ttgtgtttgt ggacgaagta ccgaaaggtc
     5041 ttaccggaaa actcgacgca agaaaaatca gagagatcct cataaaggcc aagaagggcg
     5101 gaaagtccaa attgtaagct tgatcccccg tcgacagcta gctatatcat caatttatgt
     5161 attacacata atatcgcact cagtctttca tctacggcaa tgtaccagct gatataatca
     5221 gttattgaaa tatttctgaa tttaaacttg catcaataaa tttatgtttt tgcttggact
     5281 ataatacctg acttgttatt ttatcaataa atatttaaac tatatttctt tcaagatcgc
     5341 tactagaatt cgagctcgga ggtcaacatg gtggagcacg acactctggt ctactccaaa
     5401 aatgtcaaag atacagtctc agaagatcaa agggctattg agacttttca acaaaggata
     5461 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     5521 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     5581 caagatctct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     5641 gaaaaagaag aggttccaac cacgtctaca aagcaagtgg attgatgtga catctccact
     5701 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     5761 agttcatttc atttggagag gacacgctac tgtattttta caacaattac caacaacaac
     5821 aaacaacaaa caacattaca attactattt acaattacaa tggctcctaa gaagaagagg
     5881 aaggtgatga gccagttcga catcctgtgc aagacccccc ccaaggtgct ggtgcggcag
     5941 ttcgtggaga gattcgagag gcccagcggc gagaagatcg ccagctgtgc cgccgagctg
     6001 acctacctgt gctggatgat cacccacaac ggcaccgcca tcaagagggc caccttcatg
     6061 agctacaaca ccatcatcag caacagcctg agcttcgaca tcgtgaacaa gagcctgcag
     6121 ttcaagtaca agacccagaa ggccaccatc ctggaggcca gcctgaagaa gctgatcccc
     6181 gcctgggagt tcaccatcat cccttacaac ggccagaagc accagagcga catcaccgac
     6241 atcgtgtcca gcctgcagct gcagttcgag agcagcgagg aggccgacaa gggcaacagc
     6301 cacagcaaga agatgctgaa ggccctgctg tccgagggcg agagcatctg ggagatcacc
     6361 gagaagatcc tgaacagctt cgagtacacc agcaggttca ccaagaccaa gaccctgtac
     6421 cagttcctgt tcctggccac attcatcaac tgcggcaggt tcagcgacat caagaacgtg
     6481 gaccccaaga gcttcaagct ggtgcagaac aagtacctgg gcgtgatcat tcagtgcctg
     6541 gtgaccgaga ctaagacaag cgtgtccagg taagtttctg cttctacctt tgatatatat
     6601 ataataatta tcattaatta gtagtaatat aatatttcaa atattttttt caaaataaaa
     6661 gaatgtagta tatagcaatt gcttttctgt agtttataag tgtgtatatt ttaatttata
     6721 acttttctaa tatatgacca aaatttgttg atgtgcaggc acatctactt tttcagcgcc
     6781 agaggcagga tcgaccccct ggtgtacctg gacgagttcc tgaggaacag cgagcccgtg
     6841 ctgaagagag tgaacaggac cggcaacagc agcagcaaca agcaggagta ccagctgctg
     6901 aaggacaacc tggtgcgcag ctacaacaag gccctgaaga agaacgcccc ctaccccatc
     6961 ttcgctatca agaacggccc taagagccac atcggcaggc acctgatgac cagctttctg
     7021 agcatgaagg gcctgaccga gctgacaaac gtggtgggca actggagcga caagagggcc
     7081 tccgccgtgg ccaggaccac ctacacccac cagatcaccg ccatccccga ccactacttc
     7141 gccctggtgt ccaggtacta cgcctacgac cccatcagca aggagatgat cgccctgaag
     7201 gacgagacta accccatcga ggagtggcag cacatcgagc agctgaaggg cagcgccgag
     7261 ggcagcatca gataccccgc ctggaacggc atcatcagcc aggaggtgct ggactacctg
     7321 agcagctaca tcaacaggcg gatctgagct tctctagcta gagtcgatcg acaagctcga
     7381 gtttctccat aataatgtgt gagtagttcc cagataaggg aattagggtt cctatagggt
     7441 ttcgctcatg tgttgagcat ataagaaacc cttagtatgt atttgtattt gtaaaatact
     7501 tctatcaata aaatttctaa ttcctaaaac caaaatccag tactaaaatc cagatcgctt
     7561 tactgagacc gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     7621 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     7681 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     7741 catgattacg ccaagcttgc atgcctgcag gtcgactcta gaggatcccc gggtaccgag
     7801 ctcgaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     7861 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     7921 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta
     7981 ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat
     8041 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
     8101 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgacgg tctcagggac
     8161 acgaagtgat ccgtttaaac tatcagtgtt tgacaggata tattggcggg taaacctaag
     8221 agaaaagagc gtttattaga ataatcggat atttaaaagg gcgtgaaaag gtttatccgt
     8281 tcgtccattt gtatgtgcca gccgcctttg cgacgctcac cgggctggtt gccctcgccg
     8341 ctgggctggc ggccgtctat ggccctgcaa acgcgccaga aacgccgtcg aagccgtgtg
     8401 cgagacaccg cggccgccgg cgttgtggat acctcgcgga aaacttggcc ctcactgaca
     8461 gatgaggggc ggacgttgac acttgagggg ccgactcacc cggcgcggcg ttgacagatg
     8521 aggggcaggc tcgatttcgg ccggcgacgt ggagctggcc agcctcgcaa atcggcgaaa
     8581 acgcctgatt ttacgcgagt ttcccacaga tgatgtggac aagcctgggg ataagtgccc
     8641 tgcggtattg acacttgagg ggcgcgacta ctgacagatg aggggcgcga tccttgacac
     8701 ttgaggggca gagtgctgac agatgagggg cgcacctatt gacatttgag gggctgtcca
     8761 caggcagaaa atccagcatt tgcaagggtt tccgcccgtt tttcggccac cgctaacctg
     8821 tcttttaacc tgcttttaaa ccaatattta taaaccttgt ttttaaccag ggctgcgccc
     8881 tgtgcgcgtg accgcgcacg ccgaaggggg gtgccccccc ttctcgaacc ctcccggccc
     8941 gctaacgcgg gcctcccatc cccccagggg ctgcgcccct cggccgcgaa cggcctcacc
     9001 ccaaaaatgg cagcgctggc caattcccga gtgcgcggaa cccctatttg tttatttttc
     9061 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     9121 tattgaaaaa ggaagagtat ggctaaaatg agaatatcac cggaattgaa aaaactgatc
     9181 gaaaaatacc gctgcgtaaa agatacggaa ggaatgtctc ctgctaaggt atataagctg
     9241 gtgggagaaa atgaaaacct atatttaaaa atgacggaca gccggtataa agggaccacc
     9301 tatgatgtgg aacgggaaaa ggacatgatg ctatggctgg aaggaaagct gcctgttcca
     9361 aaggtcctgc actttgaacg gcatgatggc tggagcaatc tgctcatgag tgaggccgat
     9421 ggcgtccttt gctcggaaga gtatgaagat gaacaaagcc ctgaaaagat tatcgagctg
     9481 tatgcggagt gcatcaggct ctttcactcc atcgacatat cggattgtcc ctatacgaat
     9541 agcttagaca gccgcttagc cgaattggat tacttactga ataacgatct ggccgatgtg
     9601 gattgcgaaa actgggaaga ggacactcca tttaaagatc cgcgcgagct gtatgatttt
     9661 ttaaagacgg aaaagcccga agaggaactt gtcttttccc acggcgacct gggagacagc
     9721 aacatctttg tgaaagatgg caaagtaagt ggctttattg atcttgggag aagcggcagg
     9781 gcggacaagt ggtatgacat tgccttctgc gtccggtcgc tcagggagga tatcggggaa
     9841 gaacagtatg tcgagctatt ttttgactta ctggggatca agcctgattg ggagaaaata
     9901 aaatattata ttttactgga tgaattgttt tagctgtcag accaagttta ctcatatata
     9961 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
    10021 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
    10081 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
    10141 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
    10201 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
    10261 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
    10321 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
    10381 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
    10441 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
    10501 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
    10561 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
    10621 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
    10681 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcaga tcctagatgt
    10741 ggcgcaacga tgccggcgac aagcaggagc gcaccgactt cttccgcatc aagtgttttg
    10801 gctctcaggc cgaggcccac ggcaagtatt tgggcaaggg gtcgctggta ttcgtgcagg
    10861 gcaagattcg gaataccaag tacgagaagg acggccagac ggtctacggg accgacttca
    10921 ttgccgataa ggtggattat ctggacacca aggcaccagg cgggtcaaat caggaataag
    10981 ggcacattgc cccggcgtga gtcggggcaa tcccgcaagg agggtgaatg aatcggacgt
    11041 ttgaccggaa ggcatacagg caagaactga tcgacgcggg gttttccgcc gaggatgccg
    11101 aaaccatcgc aagccgcacc gtcatgcgtg cgccccgcga aaccttccag tccgtcggct
    11161 cgatggtcca gcaagctacg gccaagatcg agcgcgacag cgtgcaactg gctccccctg
    11221 ccctgcccgc gccatcggcc gccgtggagc gttcgcgtcg tcttgaacag gaggcggcag
    11281 gtttggcgaa gtcgatgacc atcgacacgc gaggaactat gacgaccaag aagcgaaaaa
    11341 ccgccggcga ggacctggca aaacaggtca gcgaggccaa gcaggccgcg ttgctgaaac
    11401 acacgaagca gcagatcaag gaaatgcagc tttccttgtt cgatattgcg ccgtggccgg
    11461 acacgatgcg agcgatgcca aacgacacgg cccgctctgc cctgttcacc acgcgcaaca
    11521 agaaaatccc gcgcgaggcg ctgcaaaaca aggtcatttt ccacgtcaac aaggacgtga
    11581 agatcaccta caccggcgtc gagctgcggg ccgacgatga cgaactggtg tggcagcagg
    11641 tgttggagta cgcgaagcgc acccctatcg gcgagccgat caccttcacg ttctacgagc
    11701 tttgccagga cctgggctgg tcgatcaatg gccggtatta cacgaaggcc gaggaatgcc
    11761 tgtcgcgcct acaggcgacg gcgatgggct tcacgtccga ccgcgttggg cacctggaat
    11821 cggtgtcgct gctgcaccgc ttccgcgtcc tggaccgtgg caagaaaacg tcccgttgcc
    11881 aggtcctgat cgacgaggaa atcgtcgtgc tgtttgctgg cgaccactac acgaaattca
    11941 tatgggagaa gtaccgcaag ctgtcgccga cggcccgacg gatgttcgac tatttcagct
    12001 cgcaccggga gccgtacccg ctcaagctgg aaaccttccg cctcatgtgc ggatcggatt
    12061 ccacccgcgt gaagaagtgg cgcgagcagg tcggcgaagc ctgcgaagag ttgcgaggca
    12121 gcggcctggt ggaacacgcc tgggtcaatg atgacctggt gcattgcaaa cgctagggcc
    12181 ttgtggggtc agttccggct gggggttcag cagcccctgc tcggatctgt tggaccggac
    12241 agtagtcatg gttgatgggc tgcctgtatc gagtggtgat tttgtgccga gctgccggtc
    12301 ggggagctgt tggctggctg gtggcaggat atattgtggt gtaaacaaat tgacgcttag
    12361 acaacttaat aacacattgc ggacgttttt aatgtactgg ggttgaacac tct
//
