LOCUS       sequence-377980-       10193 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To act as the off state for testing of the alt B3 recombinase..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10193)
  AUTHORS   Lloyd JPB, Ly F, Gong P, Pflueger J, Swain T, Pflueger C, Fourie E, 
            Khan MA, Kidd BN, Lister R
  TITLE     Synthetic memory circuits for stable cell reprogramming in plants.
  JOURNAL   Nat Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2.
  PUBMED    35788565
REFERENCE   2  (bases 1 to 10193)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2."
COMMENT     Sequence Label: LLP739_L2_pV01_0I_B3RT-OCS-Rluc_lacZ
FEATURES             Location/Qualifiers
     source          1..10193
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      878..1585
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     intron          1685..1873
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     polyA_signal    2901..3075
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             3463..5115
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(3529..3547)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      5135..5335
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     protein_bind    5406..5427
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5442..5447,5448..5465,5466..5472)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5480..5496
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5485..5507
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5504..5520
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5504..5520
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             5516..5839
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    5533..5589
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(5590..5607)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5590..5606)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5599..5621)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5815..5834)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    5971..5995
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      6083..6793
                     /label=oriV
                     /note="incP origin of replication"
     promoter        6814..6918
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6919..7713
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      7884..8472
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8373..8392
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             8808..9956
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    10102..10126
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgaatt cggatccgga gtcgacaaaa tttagaacga acttaattat gatctcaaat
       61 acattgatac atatctcatc tagatctagg ttatcattat gtaagaaagt tttgacgaat
      121 atggcacgac aaaatggcta gactcgatgt aattggtatc tcaactcaac attatactta
      181 taccaaacat tagttagaca aaatttaaac aactattttt tatgtatgca agagtcagca
      241 tatgtataat tgattcagaa tcgttttgac gagttcggat gtagtagtag ccattattta
      301 atgtacatac taatcgtgaa tagtgaatat gatgaaacat tgtatcttat tgtataaata
      361 tccataaaca catcatgaaa gacactttct ttcacggtct gaattaatta tgatacaatt
      421 ctaatagaaa acgaattaaa ttacgttgaa ttgtatgaaa tctaattgaa caagccaacc
      481 acgacgacga ctaacgttgc ctggattgac tcggtttaag ttaaccacta aaaaaacgga
      541 gctgtcatgt aacacgcgga tcgagcaggt cacagtcatg aagccatcaa agcaaaagaa
      601 ctaatccaag ggctgagatg attaattagt ttaaaaatta gttaacacga gggaaaaggc
      661 tgtctgacag ccaggtcacg ttatctttac ctgtggtcga aatgattcgt gtctgtcgat
      721 tttaattatt tttttgaaag gccgaaaata aagttgtaag agataaaccc gcctatataa
      781 attcatatat tttcctctcc gctttgaata ctgtattttt acaacaatta ccaacaacaa
      841 caaaggttgc ttaagaataa gtaattctta agcaaccctg ctttaatgag atatgcgaga
      901 agcctatgat cgcatgatat ttgctttcaa ttctgttgtg cacgttgtaa aaaacctgag
      961 catgtgtagc tcagatcctt accgccggtt tcggttcatt ctaatgaata tatcacccgt
     1021 tactatcgta tttttatgaa taatattctc cgttcaattt actgattgta ccctactact
     1081 tatatgtaca atattaaaat gaaaacaata tattgtgctg aataggttta tagcgacatc
     1141 tatgatagag cgccacaata acaaacaatt gcgttttatt attacaaatc caattttaaa
     1201 aaaagcggca gaaccggtca aacctaaaag actgattaca taaatcttat tcaaatttca
     1261 aaagtgcccc aggggctagt atctacgaca caccgagcgg cgaactaata acgctcactg
     1321 aagggaactc cggttccccg ccggcgcgca tgggtgagat tccttgaagt tgagtattgg
     1381 ccgtccgctc taccgaaagt tacgggcacc attcaacccg gtccagcacg gcggccgggt
     1441 aaccgacttg ctgccccgag aattatgcag catttttttg gtgtatgtgg gccccaaatg
     1501 aagtgcaggt caaaccttga cagtgacgac aaatcgttgg gcgggtccag ggcgaatttt
     1561 gcgacaacat gtcgaggctc agcagggttg cttaagaata agtaattctt aagcaaccca
     1621 acaaacaaca ttacaattac tatttacaat tacaatgact tcaaaggtgt atgatcctga
     1681 gcaggtaagt ttctgcttct acctttgata tatatataat aattatcatt aattagtagt
     1741 aatataatat ttcaaatatt tttttcaaaa taaaagaatg tagtatatag caattgcttt
     1801 tctgtagttt ataagtgtgt atattttaat ttataacttt tctaatatat gaccaaaatt
     1861 tgttgatgtg cagagaaaaa gaatgataac tggtccacaa tggtgggcaa gatgtaaaca
     1921 gatgaacgtg cttgattctt tcattaatta ctacgattca gaaaagcacg ctgagaatgc
     1981 agttatcttt cttcacggaa acgctgcatc ttcatatttg tggagacatg ttgtgcctca
     2041 cattgaacca gtggctaggt gtattatccc agatcttatc ggaatgggaa agtctggtaa
     2101 atctggaaat ggttcttaca gacttttgga tcattataag tacctcacag cttggttcga
     2161 acttctcaac ttacctaaga agattatttt cgttggacac gattggggtg cttgcttggc
     2221 attccattac tcatacgaac accaagataa gatcaaagct atagttcatg cagagagtgt
     2281 tgtggatgtg atagaatctt gggatgagtg gccagatatt gaagaggata ttgctttgat
     2341 caagtctgaa gagggagaaa agatggttct cgaaaataac tttttcgtgg aaactatgct
     2401 tccttcaaag attatgagaa aattggaacc agaagagttt gctgcatatc tcgaaccttt
     2461 caaggaaaaa ggagaggtta gaaggccaac actcagttgg cctagggaaa taccattagt
     2521 taagggaggt aaacctgatg ttgtgcagat cgtgagaaat tataacgctt accttagggc
     2581 atcagatgat ttgcctaaga tgttcatcga gtcagatcca ggatttttca gtaatgctat
     2641 agttgaaggt gcaaagaaat ttccaaacac cgagttcgtt aaggtgaaag gattgcattt
     2701 ctcacaagaa gatgctcctg atgagatggg aaagtatatt aagtcttttg ttgaaagagt
     2761 gctcaaaaac gagcagggtt ctcgatctga tgcttctgaa gtaacgtcaa caagctccca
     2821 gtctgaggta tgcacagtgg aaacgccagg ttgtgttggt tcttaagctt ctctagctag
     2881 agtcgatcga caagctcgag tttctccata ataatgtgtg agtagttccc agataaggga
     2941 attagggttc ctatagggtt tcgctcatgt gttgagcata taagaaaccc ttagtatgta
     3001 tttgtatttg taaaatactt ctatcaataa aatttctaat tcctaaaacc aaaatccagt
     3061 actaaaatcc agatcgctgc aagaattcaa gcttggagcc aacactcgaa tccccacccg
     3121 ttggaccaaa cccggctcat taagcgtcgg ttcagattta tttcctttat ttaaaaaaaa
     3181 ggaaagggta aaaaatagaa aattggaaac agttaaagcc caaaattgta atttaccgag
     3241 aattgtaaat ttacctgaaa accctacgct atagtttcga ctataaatac caaacttagg
     3301 acctcacttc agaatcccct cgtcgctgcg tctctctccc gcaaccttcg attttcgttt
     3361 attcgcatcc atcggagaga gaaaacaatc aataagcgac ctactacaaa caagtaagta
     3421 agagaaaaac caaaagaaga agagaaacaa caagaagaag taatggagga cgccaaaaac
     3481 ataaagaaag gcccggcgcc attctatcct ctagaggatg gaaccgctgg agagcaactg
     3541 cataaggcta tgaagagata cgccctggtt cctggaacaa ttgcttttac agatgcacat
     3601 atcgaggtga acatcacgta cgcggaatac ttcgaaatgt ccgttcggtt ggcagaagct
     3661 atgaaacgat atgggctgaa tacaaatcac agaatcgtcg tatgcagtga aaactctctt
     3721 caattcttta tgccggtgtt gggcgcgtta tttatcggag ttgcagttgc gcccgcgaac
     3781 gacatttata atgaacgtga attgctcaac agtatgaaca tttcgcagcc taccgtagtg
     3841 tttgtttcca aaaaggggtt gcaaaaaatt ttgaacgtgc aaaaaaaatt accaataatc
     3901 cagaaaatta ttatcatgga ttctaaaacg gattaccagg gatttcagtc gatgtacacg
     3961 ttcgtcacat ctcatctacc tcccggtttt aatgaatacg attttgtacc agagtccttt
     4021 gatcgtgaca aaacaattgc actgataatg aattcctctg gatctactgg gttacctaag
     4081 ggtgtggccc ttccgcatag aactgcctgc gtcagattct cgcatgccag agatcctatt
     4141 tttggcaatc aaatcattcc ggatactgcg attttaagtg ttgttccatt ccatcacggt
     4201 tttggaatgt ttactacact cggatatttg atatgtggat ttcgagtcgt cttaatgtat
     4261 agatttgaag aagagctgtt tttacgatcc cttcaggatt acaaaattca aagtgcgttg
     4321 ctagtaccaa ccctattttc attcttcgcc aaaagcactc tgattgacaa atacgattta
     4381 tctaatttac acgaaattgc ttctgggggc gcacctcttt cgaaagaagt cggggaagcg
     4441 gttgcaaaac gcttccatct tccagggata cgacaaggat atgggctcac tgagactaca
     4501 tcagctattc tgattacacc cgagggggat gataaaccgg gcgcggtcgg taaagttgtt
     4561 ccattttttg aagcgaaggt tgtggatctg gataccggga aaacgctggg cgttaatcag
     4621 agaggcgaat tatgtgtcag aggacctatg attatgtccg gttatgtaaa caatccggaa
     4681 gcgaccaacg ccttgattga caaggatgga tggctacatt ctggagacat agcttactgg
     4741 gacgaggacg aacacttctt catagttgac cgcttgaagt ctttaattaa atacaaagga
     4801 tatcaggtgg cccccgctga attggaatcg atattgttac aacaccccaa catcttcgac
     4861 gcgggcgtgg caggtctgcc cgacgatgac gccggtgaac ttcccgccgc cgttgttgtt
     4921 ttggagcacg gaaagacgat gacggaaaaa gagatcgtgg attacgtcgc cagtcaagta
     4981 acaaccgcga aaaagttgcg cggaggagtt gtgtttgtgg acgaagtacc gaaaggtctt
     5041 accggaaaac tcgacgcaag aaaaatcaga gagatcctca taaaggccaa gaagggcgga
     5101 aagtccaaat tgtaagcttg atcccccgtc gacagctagc tatatcatca atttatgtat
     5161 tacacataat atcgcactca gtctttcatc tacggcaatg taccagctga tataatcagt
     5221 tattgaaata tttctgaatt taaacttgca tcaataaatt tatgtttttg cttggactat
     5281 aatacctgac ttgttatttt atcaataaat atttaaacta tatttctttc aagatcgcta
     5341 ctatgagacc gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     5401 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     5461 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     5521 catgattacg ccaagcttgc atgcctgcag gtcgactcta gaggatcccc gggtaccgag
     5581 ctcgaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     5641 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     5701 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta
     5761 ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat
     5821 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
     5881 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgacgg tctcagggac
     5941 acgaagtgat ccgtttaaac tatcagtgtt tgacaggata tattggcggg taaacctaag
     6001 agaaaagagc gtttattaga ataatcggat atttaaaagg gcgtgaaaag gtttatccgt
     6061 tcgtccattt gtatgtgcca gccgcctttg cgacgctcac cgggctggtt gccctcgccg
     6121 ctgggctggc ggccgtctat ggccctgcaa acgcgccaga aacgccgtcg aagccgtgtg
     6181 cgagacaccg cggccgccgg cgttgtggat acctcgcgga aaacttggcc ctcactgaca
     6241 gatgaggggc ggacgttgac acttgagggg ccgactcacc cggcgcggcg ttgacagatg
     6301 aggggcaggc tcgatttcgg ccggcgacgt ggagctggcc agcctcgcaa atcggcgaaa
     6361 acgcctgatt ttacgcgagt ttcccacaga tgatgtggac aagcctgggg ataagtgccc
     6421 tgcggtattg acacttgagg ggcgcgacta ctgacagatg aggggcgcga tccttgacac
     6481 ttgaggggca gagtgctgac agatgagggg cgcacctatt gacatttgag gggctgtcca
     6541 caggcagaaa atccagcatt tgcaagggtt tccgcccgtt tttcggccac cgctaacctg
     6601 tcttttaacc tgcttttaaa ccaatattta taaaccttgt ttttaaccag ggctgcgccc
     6661 tgtgcgcgtg accgcgcacg ccgaaggggg gtgccccccc ttctcgaacc ctcccggccc
     6721 gctaacgcgg gcctcccatc cccccagggg ctgcgcccct cggccgcgaa cggcctcacc
     6781 ccaaaaatgg cagcgctggc caattcccga gtgcgcggaa cccctatttg tttatttttc
     6841 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     6901 tattgaaaaa ggaagagtat ggctaaaatg agaatatcac cggaattgaa aaaactgatc
     6961 gaaaaatacc gctgcgtaaa agatacggaa ggaatgtctc ctgctaaggt atataagctg
     7021 gtgggagaaa atgaaaacct atatttaaaa atgacggaca gccggtataa agggaccacc
     7081 tatgatgtgg aacgggaaaa ggacatgatg ctatggctgg aaggaaagct gcctgttcca
     7141 aaggtcctgc actttgaacg gcatgatggc tggagcaatc tgctcatgag tgaggccgat
     7201 ggcgtccttt gctcggaaga gtatgaagat gaacaaagcc ctgaaaagat tatcgagctg
     7261 tatgcggagt gcatcaggct ctttcactcc atcgacatat cggattgtcc ctatacgaat
     7321 agcttagaca gccgcttagc cgaattggat tacttactga ataacgatct ggccgatgtg
     7381 gattgcgaaa actgggaaga ggacactcca tttaaagatc cgcgcgagct gtatgatttt
     7441 ttaaagacgg aaaagcccga agaggaactt gtcttttccc acggcgacct gggagacagc
     7501 aacatctttg tgaaagatgg caaagtaagt ggctttattg atcttgggag aagcggcagg
     7561 gcggacaagt ggtatgacat tgccttctgc gtccggtcgc tcagggagga tatcggggaa
     7621 gaacagtatg tcgagctatt ttttgactta ctggggatca agcctgattg ggagaaaata
     7681 aaatattata ttttactgga tgaattgttt tagctgtcag accaagttta ctcatatata
     7741 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     7801 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     7861 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     7921 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     7981 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     8041 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     8101 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     8161 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     8221 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     8281 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     8341 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     8401 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     8461 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcaga tcctagatgt
     8521 ggcgcaacga tgccggcgac aagcaggagc gcaccgactt cttccgcatc aagtgttttg
     8581 gctctcaggc cgaggcccac ggcaagtatt tgggcaaggg gtcgctggta ttcgtgcagg
     8641 gcaagattcg gaataccaag tacgagaagg acggccagac ggtctacggg accgacttca
     8701 ttgccgataa ggtggattat ctggacacca aggcaccagg cgggtcaaat caggaataag
     8761 ggcacattgc cccggcgtga gtcggggcaa tcccgcaagg agggtgaatg aatcggacgt
     8821 ttgaccggaa ggcatacagg caagaactga tcgacgcggg gttttccgcc gaggatgccg
     8881 aaaccatcgc aagccgcacc gtcatgcgtg cgccccgcga aaccttccag tccgtcggct
     8941 cgatggtcca gcaagctacg gccaagatcg agcgcgacag cgtgcaactg gctccccctg
     9001 ccctgcccgc gccatcggcc gccgtggagc gttcgcgtcg tcttgaacag gaggcggcag
     9061 gtttggcgaa gtcgatgacc atcgacacgc gaggaactat gacgaccaag aagcgaaaaa
     9121 ccgccggcga ggacctggca aaacaggtca gcgaggccaa gcaggccgcg ttgctgaaac
     9181 acacgaagca gcagatcaag gaaatgcagc tttccttgtt cgatattgcg ccgtggccgg
     9241 acacgatgcg agcgatgcca aacgacacgg cccgctctgc cctgttcacc acgcgcaaca
     9301 agaaaatccc gcgcgaggcg ctgcaaaaca aggtcatttt ccacgtcaac aaggacgtga
     9361 agatcaccta caccggcgtc gagctgcggg ccgacgatga cgaactggtg tggcagcagg
     9421 tgttggagta cgcgaagcgc acccctatcg gcgagccgat caccttcacg ttctacgagc
     9481 tttgccagga cctgggctgg tcgatcaatg gccggtatta cacgaaggcc gaggaatgcc
     9541 tgtcgcgcct acaggcgacg gcgatgggct tcacgtccga ccgcgttggg cacctggaat
     9601 cggtgtcgct gctgcaccgc ttccgcgtcc tggaccgtgg caagaaaacg tcccgttgcc
     9661 aggtcctgat cgacgaggaa atcgtcgtgc tgtttgctgg cgaccactac acgaaattca
     9721 tatgggagaa gtaccgcaag ctgtcgccga cggcccgacg gatgttcgac tatttcagct
     9781 cgcaccggga gccgtacccg ctcaagctgg aaaccttccg cctcatgtgc ggatcggatt
     9841 ccacccgcgt gaagaagtgg cgcgagcagg tcggcgaagc ctgcgaagag ttgcgaggca
     9901 gcggcctggt ggaacacgcc tgggtcaatg atgacctggt gcattgcaaa cgctagggcc
     9961 ttgtggggtc agttccggct gggggttcag cagcccctgc tcggatctgt tggaccggac
    10021 agtagtcatg gttgatgggc tgcctgtatc gagtggtgat tttgtgccga gctgccggtc
    10081 ggggagctgt tggctggctg gtggcaggat atattgtggt gtaaacaaat tgacgcttag
    10141 acaacttaat aacacattgc ggacgttttt aatgtactgg ggttgaacac tct
//
