LOCUS       sequence-377983-       12009 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Off state for the second generation of NOT gates, which target the 
            Rluc promoter to repress expression via its B3RT sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12009)
  AUTHORS   Lloyd JPB, Ly F, Gong P, Pflueger J, Swain T, Pflueger C, Fourie E, 
            Khan MA, Kidd BN, Lister R
  TITLE     Synthetic memory circuits for stable cell reprogramming in plants.
  JOURNAL   Nat Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2.
  PUBMED    35788565
REFERENCE   2  (bases 1 to 12009)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2."
COMMENT     Sequence Label: LLP758_L2pV3_NOT_B3RT_Rluc_1IB_lacZ
FEATURES             Location/Qualifiers
     source          1..12009
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    890..945
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     intron          977..1165
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     polyA_signal    2193..2367
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             2755..4407
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(2821..2839)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      4427..4627
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     misc_feature    4970..5025
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             5030..5050
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          5828..6016
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     polyA_signal    6978..7152
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     protein_bind    7222..7243
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7258..7263,7264..7281,7282..7288)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7296..7312
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7301..7323
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7320..7336
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7320..7336
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             7332..7655
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    7349..7405
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(7406..7423)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7406..7422)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7415..7437)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(7631..7650)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    7787..7811
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      7899..8609
                     /label=oriV
                     /note="incP origin of replication"
     promoter        8630..8734
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8735..9529
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      9700..10288
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10189..10208
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             10624..11772
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    11918..11942
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgaatt cggatccgga gggttgctta agaataagta attcttaagc aacctcgaca
       61 aaatttagaa cgaacttaat tatgatctca aatacattga tacatatctc atctagatct
      121 aggttatcat tatgtaagaa agttttgacg aatatggcac gacaaaatgg ctagactcga
      181 tgtaattggt atctcaactc aacattatac ttataccaaa cattagttag acaaaattta
      241 aacaactatt ttttatgtat gcaagagtca gcatatgtat aattgattca gaatcgtttt
      301 gacgagttcg gatgtagtag tagccattat ttaatgtaca tactaatcgt gaatagtgaa
      361 tatgatgaaa cattgtatct tattgtataa atatccataa acacatcatg aaagacactt
      421 tctttcacgg tctgaattaa ttatgataca attctaatag aaaacgaatt aaattacgtt
      481 gaattgtatg aaatctaatt gaacaagcca accacgacga cgactaacgt tgcctggatt
      541 gactcggttt aagttaacca ctaaaaaaac ggagctgtca tgtaacacgc ggatcgagca
      601 ggtcacagtc atgaagccat caaagcaaaa gaactaatcc aagggctgag atgattaatt
      661 agtttaaaaa ttagttaaca cgagggaaaa ggctgtctga cagccaggtc acgttatctt
      721 tacctgtggt cgaaatgatt cgtgtctgtc gattttaatt atttttttga aaggccgaaa
      781 ataaagttgt aagagataaa cccgcctata taaattcata tattttcctc tccgctttga
      841 aggttgctta agaataagta attcttaagc aacctactgt atttttacaa caattaccaa
      901 caacaacaaa caacaaacaa cattacaatt actatttaca attacaatga cttcaaaggt
      961 gtatgatcct gagcaggtaa gtttctgctt ctacctttga tatatatata ataattatca
     1021 ttaattagta gtaatataat atttcaaata tttttttcaa aataaaagaa tgtagtatat
     1081 agcaattgct tttctgtagt ttataagtgt gtatatttta atttataact tttctaatat
     1141 atgaccaaaa tttgttgatg tgcagagaaa aagaatgata actggtccac aatggtgggc
     1201 aagatgtaaa cagatgaacg tgcttgattc tttcattaat tactacgatt cagaaaagca
     1261 cgctgagaat gcagttatct ttcttcacgg aaacgctgca tcttcatatt tgtggagaca
     1321 tgttgtgcct cacattgaac cagtggctag gtgtattatc ccagatctta tcggaatggg
     1381 aaagtctggt aaatctggaa atggttctta cagacttttg gatcattata agtacctcac
     1441 agcttggttc gaacttctca acttacctaa gaagattatt ttcgttggac acgattgggg
     1501 tgcttgcttg gcattccatt actcatacga acaccaagat aagatcaaag ctatagttca
     1561 tgcagagagt gttgtggatg tgatagaatc ttgggatgag tggccagata ttgaagagga
     1621 tattgctttg atcaagtctg aagagggaga aaagatggtt ctcgaaaata actttttcgt
     1681 ggaaactatg cttccttcaa agattatgag aaaattggaa ccagaagagt ttgctgcata
     1741 tctcgaacct ttcaaggaaa aaggagaggt tagaaggcca acactcagtt ggcctaggga
     1801 aataccatta gttaagggag gtaaacctga tgttgtgcag atcgtgagaa attataacgc
     1861 ttaccttagg gcatcagatg atttgcctaa gatgttcatc gagtcagatc caggattttt
     1921 cagtaatgct atagttgaag gtgcaaagaa atttccaaac accgagttcg ttaaggtgaa
     1981 aggattgcat ttctcacaag aagatgctcc tgatgagatg ggaaagtata ttaagtcttt
     2041 tgttgaaaga gtgctcaaaa acgagcaggg ttctcgatct gatgcttctg aagtaacgtc
     2101 aacaagctcc cagtctgagg tatgcacagt ggaaacgcca ggttgtgttg gttcttaagc
     2161 ttctctagct agagtcgatc gacaagctcg agtttctcca taataatgtg tgagtagttc
     2221 ccagataagg gaattagggt tcctataggg tttcgctcat gtgttgagca tataagaaac
     2281 ccttagtatg tatttgtatt tgtaaaatac ttctatcaat aaaatttcta attcctaaaa
     2341 ccaaaatcca gtactaaaat ccagatcgct gcaagaattc aagcttggag ccaacactcg
     2401 aatccccacc cgttggacca aacccggctc attaagcgtc ggttcagatt tatttccttt
     2461 atttaaaaaa aaggaaaggg taaaaaatag aaaattggaa acagttaaag cccaaaattg
     2521 taatttaccg agaattgtaa atttacctga aaaccctacg ctatagtttc gactataaat
     2581 accaaactta ggacctcact tcagaatccc ctcgtcgctg cgtctctctc ccgcaacctt
     2641 cgattttcgt ttattcgcat ccatcggaga gagaaaacaa tcaataagcg acctactaca
     2701 aacaagtaag taagagaaaa accaaaagaa gaagagaaac aacaagaaga agtaatggag
     2761 gacgccaaaa acataaagaa aggcccggcg ccattctatc ctctagagga tggaaccgct
     2821 ggagagcaac tgcataaggc tatgaagaga tacgccctgg ttcctggaac aattgctttt
     2881 acagatgcac atatcgaggt gaacatcacg tacgcggaat acttcgaaat gtccgttcgg
     2941 ttggcagaag ctatgaaacg atatgggctg aatacaaatc acagaatcgt cgtatgcagt
     3001 gaaaactctc ttcaattctt tatgccggtg ttgggcgcgt tatttatcgg agttgcagtt
     3061 gcgcccgcga acgacattta taatgaacgt gaattgctca acagtatgaa catttcgcag
     3121 cctaccgtag tgtttgtttc caaaaagggg ttgcaaaaaa ttttgaacgt gcaaaaaaaa
     3181 ttaccaataa tccagaaaat tattatcatg gattctaaaa cggattacca gggatttcag
     3241 tcgatgtaca cgttcgtcac atctcatcta cctcccggtt ttaatgaata cgattttgta
     3301 ccagagtcct ttgatcgtga caaaacaatt gcactgataa tgaattcctc tggatctact
     3361 gggttaccta agggtgtggc ccttccgcat agaactgcct gcgtcagatt ctcgcatgcc
     3421 agagatccta tttttggcaa tcaaatcatt ccggatactg cgattttaag tgttgttcca
     3481 ttccatcacg gttttggaat gtttactaca ctcggatatt tgatatgtgg atttcgagtc
     3541 gtcttaatgt atagatttga agaagagctg tttttacgat cccttcagga ttacaaaatt
     3601 caaagtgcgt tgctagtacc aaccctattt tcattcttcg ccaaaagcac tctgattgac
     3661 aaatacgatt tatctaattt acacgaaatt gcttctgggg gcgcacctct ttcgaaagaa
     3721 gtcggggaag cggttgcaaa acgcttccat cttccaggga tacgacaagg atatgggctc
     3781 actgagacta catcagctat tctgattaca cccgaggggg atgataaacc gggcgcggtc
     3841 ggtaaagttg ttccattttt tgaagcgaag gttgtggatc tggataccgg gaaaacgctg
     3901 ggcgttaatc agagaggcga attatgtgtc agaggaccta tgattatgtc cggttatgta
     3961 aacaatccgg aagcgaccaa cgccttgatt gacaaggatg gatggctaca ttctggagac
     4021 atagcttact gggacgagga cgaacacttc ttcatagttg accgcttgaa gtctttaatt
     4081 aaatacaaag gatatcaggt ggcccccgct gaattggaat cgatattgtt acaacacccc
     4141 aacatcttcg acgcgggcgt ggcaggtctg cccgacgatg acgccggtga acttcccgcc
     4201 gccgttgttg ttttggagca cggaaagacg atgacggaaa aagagatcgt ggattacgtc
     4261 gccagtcaag taacaaccgc gaaaaagttg cgcggaggag ttgtgtttgt ggacgaagta
     4321 ccgaaaggtc ttaccggaaa actcgacgca agaaaaatca gagagatcct cataaaggcc
     4381 aagaagggcg gaaagtccaa attgtaagct tgatcccccg tcgacagcta gctatatcat
     4441 caatttatgt attacacata atatcgcact cagtctttca tctacggcaa tgtaccagct
     4501 gatataatca gttattgaaa tatttctgaa tttaaacttg catcaataaa tttatgtttt
     4561 tgcttggact ataatacctg acttgttatt ttatcaataa atatttaaac tatatttctt
     4621 tcaagatcgc tactagaatt cgagctcgga gccaacactc gaatccccac ccgttggacc
     4681 aaacccggct cattaagcgt cggttcagat ttatttcctt tatttaaaaa aaaggaaagg
     4741 gtaaaaaata gaaaattgga aacagttaaa gcccaaaatt gtaatttacc gagaattgta
     4801 aatttacctg aaaaccctac gctatagttt cgactataaa taccaaactt aggacctcac
     4861 ttcagaatcc cctcgtcgct gcgtctctct cccgcaacct tcgattttcg tttattcgca
     4921 tccatcggag agagaaaaca atcaataagc gacctactgt atttttacaa caattaccaa
     4981 caacaacaaa caacaaacaa cattacaatt actatttaca attacaatgc ccaagaaaaa
     5041 gcggaaggtg tccagctaca tggacctggt ggacgacgag cccgccaccc tgtaccacaa
     5101 gttcgtggaa tgcctgaagg ccggcgagaa cttctgcggc gataagctga gcggcatcat
     5161 caccatggcc attctgaagg ccatcaaggc cctgaccgaa gtgaagaaaa ccaccttcaa
     5221 caagtacaag accaccatca agcagggcct gcagtacgac gtgggcagca gcaccatcag
     5281 cttcgtgtac cacctgaagg actgcgacga gctgagcaga ggcctgagcg acgccttcga
     5341 gccctacaag ttcaagatca agagcaacaa agaggccacc agcttcaaga ccctgttcag
     5401 gggccctagc ttcggcagcc agaagaactg gcggaagaaa gaggtggacc gcgaggtgga
     5461 caacctgttc cacagcaccg agacagacga gagcatcttc aagttcatcc tgaacaccct
     5521 ggacagcatc gaaacccaga ccaacaccga ccggcagaaa accgtgctga cctttatcct
     5581 gctgatgacc ttcttcaact gctgccggaa caacgacctg atgaacgtgg accccagcac
     5641 cttcaagatc gtgaagaaca agtttgtggg ctacctgctg caggctgaag tgaagcagac
     5701 caagaccaga aagagccgga atatcttctt cttccccatc cgggaaaacc gcttcgacct
     5761 gttcctggcc ctgcacgact tcttcagaac ctgccagccc acccccaaga gcagactgag
     5821 cgatcaggta agtttctgct tctacctttg atatatatat aataattatc attaattagt
     5881 agtaatataa tatttcaaat atttttttca aaataaaaga atgtagtata tagcaattgc
     5941 ttttctgtag tttataagtg tgtatatttt aatttataac ttttctaata tatgaccaaa
     6001 atttgttgat gtgcaggtgt ccgagcagaa gtggcagctg ttccgggaca gcatggtcat
     6061 cgactacaac cggttctttc ggaagttccc cgccagcccc atcttcgcca ttaagcacgg
     6121 ccccaagtcc cacctgggcc ggcatctgat gaacagcttt ctgcacaaga acgagctgga
     6181 cagctgggcc aacagcctgg gcaattggag cagctcccag aaccagagag agagcggcgc
     6241 cagactgggc tacacacacg gcggaagaga tctgccccag cccctgtttg gcttcctggc
     6301 cggatactgc gtgcggaacg aagagggcca catcgtgggc ctgggcctgg aaaaggacat
     6361 caacgatctg ttcgacggca tcatggaccc cctgaacgag aaagaggaca ccgagatctg
     6421 cgagagctac ggcgagtggg ccaagattgt gtccaaggac gtgctgatct tcctgaagag
     6481 ataccacagc aagaacgcct gtcggagata ccagaacagc accctgtatg cccggacctt
     6541 cctgaaaacc gagagcgtga ccctgagcgg ctccaagggc agcgaggaac cttctagccc
     6601 tgtgcggatc cccatcctga gcatgggaaa ggccagcccc tccgagggaa gaaagctgag
     6661 agccagcgag cacgccaacg acgacaacga gatcgagaag atcgacagcg acagcagcca
     6721 gagcgaagag atccctatcg agatgagcga ctccgaggac gagacaaccg ccagcaacat
     6781 cagcggcatc tacctggaca tgagcaaggc caactccaac gtggtgtaca gcccccctag
     6841 ccagacaggc agagctgctg gcgccggaag aaaaagaggc gtgggaggca gacggaccgt
     6901 ggaaagcaag cggagaagag tgctggcccc catcaaccgg tgagcttctc tagctagagt
     6961 cgatcgacaa gctcgagttt ctccataata atgtgtgagt agttcccaga taagggaatt
     7021 agggttccta tagggtttcg ctcatgtgtt gagcatataa gaaaccctta gtatgtattt
     7081 gtatttgtaa aatacttcta tcaataaaat ttctaattcc taaaaccaaa atccagtact
     7141 aaaatccaga tcgctttact gagaccgcag ctggcacgac aggtttcccg actggaaagc
     7201 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     7261 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
     7321 aggaaacagc tatgaccatg attacgccaa gcttgcatgc ctgcaggtcg actctagagg
     7381 atccccgggt accgagctcg aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     7441 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     7501 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     7561 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt
     7621 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
     7681 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     7741 tgacggtctc agggacacga agtgatccgt ttaaactatc agtgtttgac aggatatatt
     7801 ggcgggtaaa cctaagagaa aagagcgttt attagaataa tcggatattt aaaagggcgt
     7861 gaaaaggttt atccgttcgt ccatttgtat gtgccagccg cctttgcgac gctcaccggg
     7921 ctggttgccc tcgccgctgg gctggcggcc gtctatggcc ctgcaaacgc gccagaaacg
     7981 ccgtcgaagc cgtgtgcgag acaccgcggc cgccggcgtt gtggatacct cgcggaaaac
     8041 ttggccctca ctgacagatg aggggcggac gttgacactt gaggggccga ctcacccggc
     8101 gcggcgttga cagatgaggg gcaggctcga tttcggccgg cgacgtggag ctggccagcc
     8161 tcgcaaatcg gcgaaaacgc ctgattttac gcgagtttcc cacagatgat gtggacaagc
     8221 ctggggataa gtgccctgcg gtattgacac ttgaggggcg cgactactga cagatgaggg
     8281 gcgcgatcct tgacacttga ggggcagagt gctgacagat gaggggcgca cctattgaca
     8341 tttgaggggc tgtccacagg cagaaaatcc agcatttgca agggtttccg cccgtttttc
     8401 ggccaccgct aacctgtctt ttaacctgct tttaaaccaa tatttataaa ccttgttttt
     8461 aaccagggct gcgccctgtg cgcgtgaccg cgcacgccga aggggggtgc ccccccttct
     8521 cgaaccctcc cggcccgcta acgcgggcct cccatccccc caggggctgc gcccctcggc
     8581 cgcgaacggc ctcaccccaa aaatggcagc gctggccaat tcccgagtgc gcggaacccc
     8641 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     8701 ataaatgctt caataatatt gaaaaaggaa gagtatggct aaaatgagaa tatcaccgga
     8761 attgaaaaaa ctgatcgaaa aataccgctg cgtaaaagat acggaaggaa tgtctcctgc
     8821 taaggtatat aagctggtgg gagaaaatga aaacctatat ttaaaaatga cggacagccg
     8881 gtataaaggg accacctatg atgtggaacg ggaaaaggac atgatgctat ggctggaagg
     8941 aaagctgcct gttccaaagg tcctgcactt tgaacggcat gatggctgga gcaatctgct
     9001 catgagtgag gccgatggcg tcctttgctc ggaagagtat gaagatgaac aaagccctga
     9061 aaagattatc gagctgtatg cggagtgcat caggctcttt cactccatcg acatatcgga
     9121 ttgtccctat acgaatagct tagacagccg cttagccgaa ttggattact tactgaataa
     9181 cgatctggcc gatgtggatt gcgaaaactg ggaagaggac actccattta aagatccgcg
     9241 cgagctgtat gattttttaa agacggaaaa gcccgaagag gaacttgtct tttcccacgg
     9301 cgacctggga gacagcaaca tctttgtgaa agatggcaaa gtaagtggct ttattgatct
     9361 tgggagaagc ggcagggcgg acaagtggta tgacattgcc ttctgcgtcc ggtcgctcag
     9421 ggaggatatc ggggaagaac agtatgtcga gctatttttt gacttactgg ggatcaagcc
     9481 tgattgggag aaaataaaat attatatttt actggatgaa ttgttttagc tgtcagacca
     9541 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     9601 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     9661 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     9721 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     9781 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     9841 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     9901 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     9961 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
    10021 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
    10081 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
    10141 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
    10201 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
    10261 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
    10321 ggcagatcct agatgtggcg caacgatgcc ggcgacaagc aggagcgcac cgacttcttc
    10381 cgcatcaagt gttttggctc tcaggccgag gcccacggca agtatttggg caaggggtcg
    10441 ctggtattcg tgcagggcaa gattcggaat accaagtacg agaaggacgg ccagacggtc
    10501 tacgggaccg acttcattgc cgataaggtg gattatctgg acaccaaggc accaggcggg
    10561 tcaaatcagg aataagggca cattgccccg gcgtgagtcg gggcaatccc gcaaggaggg
    10621 tgaatgaatc ggacgtttga ccggaaggca tacaggcaag aactgatcga cgcggggttt
    10681 tccgccgagg atgccgaaac catcgcaagc cgcaccgtca tgcgtgcgcc ccgcgaaacc
    10741 ttccagtccg tcggctcgat ggtccagcaa gctacggcca agatcgagcg cgacagcgtg
    10801 caactggctc cccctgccct gcccgcgcca tcggccgccg tggagcgttc gcgtcgtctt
    10861 gaacaggagg cggcaggttt ggcgaagtcg atgaccatcg acacgcgagg aactatgacg
    10921 accaagaagc gaaaaaccgc cggcgaggac ctggcaaaac aggtcagcga ggccaagcag
    10981 gccgcgttgc tgaaacacac gaagcagcag atcaaggaaa tgcagctttc cttgttcgat
    11041 attgcgccgt ggccggacac gatgcgagcg atgccaaacg acacggcccg ctctgccctg
    11101 ttcaccacgc gcaacaagaa aatcccgcgc gaggcgctgc aaaacaaggt cattttccac
    11161 gtcaacaagg acgtgaagat cacctacacc ggcgtcgagc tgcgggccga cgatgacgaa
    11221 ctggtgtggc agcaggtgtt ggagtacgcg aagcgcaccc ctatcggcga gccgatcacc
    11281 ttcacgttct acgagctttg ccaggacctg ggctggtcga tcaatggccg gtattacacg
    11341 aaggccgagg aatgcctgtc gcgcctacag gcgacggcga tgggcttcac gtccgaccgc
    11401 gttgggcacc tggaatcggt gtcgctgctg caccgcttcc gcgtcctgga ccgtggcaag
    11461 aaaacgtccc gttgccaggt cctgatcgac gaggaaatcg tcgtgctgtt tgctggcgac
    11521 cactacacga aattcatatg ggagaagtac cgcaagctgt cgccgacggc ccgacggatg
    11581 ttcgactatt tcagctcgca ccgggagccg tacccgctca agctggaaac cttccgcctc
    11641 atgtgcggat cggattccac ccgcgtgaag aagtggcgcg agcaggtcgg cgaagcctgc
    11701 gaagagttgc gaggcagcgg cctggtggaa cacgcctggg tcaatgatga cctggtgcat
    11761 tgcaaacgct agggccttgt ggggtcagtt ccggctgggg gttcagcagc ccctgctcgg
    11821 atctgttgga ccggacagta gtcatggttg atgggctgcc tgtatcgagt ggtgattttg
    11881 tgccgagctg ccggtcgggg agctgttggc tggctggtgg caggatatat tgtggtgtaa
    11941 acaaattgac gcttagacaa cttaataaca cattgcggac gtttttaatg tactggggtt
    12001 gaacactct
//
