LOCUS       sequence-377984-        9178 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9178)
  AUTHORS   Chu D, Kazana E, Bellanger N, Singh T, Tuite MF, von der Haar T
  TITLE     Translation elongation can control translation initiation on 
            eukaryotic mRNAs.
  JOURNAL   EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.1002/embj.201385651. Epub 
            2013 Dec 19.
  PUBMED    24357599
REFERENCE   2  (bases 1 to 9178)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/embj.201385651"; journalName: "EMBO J"; date: "2014-01-1- 
            1"; volume: "33"; issue: "1"; pages: "21-34"
COMMENT     Sequence Label: pTH728-CEN-RLuc/maxCFLuc
FEATURES             Location/Qualifiers
     source          1..9178
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    69..572
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        609..713
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(714..782,783..1574)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(932..951)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1745..2333
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2234..2253
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2487..2504
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        join(2657..2662,2663..2680,2681..2687)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2695..2711
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2700..2722
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2719..2735
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2719..2735
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2756..2774
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     terminator      complement(2822..3121)
                     /label=ADH2 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 2 (ADH2) gene"
     CDS             complement(3151..4083)
                     /codon_start=1
                     /product="luciferase from the anthozoan coelenterate 
                     Renilla reniformis (sea pansy)"
                     /label=Rluc
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     promoter        4495..5138
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     5031..5054
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     terminator      6945..7132
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(7000..7024)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(7197..7216)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7198..7216)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7223..7240)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7223..7239)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7232..7254)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7380..7835
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7467..7486)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7677..7698
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(7966..8769)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(8770..8985)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(9009..9028)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9128..9150
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgga tcgcttgcct gtaacttaca cgcgcctcgt atcttttaat gatggaataa
      121 tttgggaatt tactctgtgt ttatttattt ttatgttttg tatttggatt ttagaaagta
      181 aataaagaag gtagaagagt tacggaatga agaaaaaaaa ataaacaaag gtttaaaaaa
      241 tttcaacaaa aagcgtactt tacatatata tttattagac aagaaaagca gattaaatag
      301 atatacattc gattaacgat aagtaaaatg taaaatcaca ggattttcgt gtgtggtctt
      361 ctacacagac aagatgaaac aattcggcat taatacctga gagcaggaag agcaagataa
      421 aaggtagtat ttgttggcga tccccctaga gtcttttaca tcttcggaaa acaaaaacta
      481 ttttttcttt aatttctttt tttactttct atttttaatt tatatattta tattaaaaaa
      541 tttaaattat aattattttt atagcacgtg atgaaaagga cccaggtggc acttttcggg
      601 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
      661 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
      721 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
      781 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
      841 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
      901 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
      961 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     1021 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     1081 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     1141 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     1201 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     1261 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     1321 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     1381 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     1441 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     1501 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     1561 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     1621 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     1681 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     1741 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     1801 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     1861 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     1921 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     1981 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     2041 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     2101 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     2161 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     2221 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     2281 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     2341 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     2401 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     2461 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     2521 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     2581 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtaagt tagctcactc
     2641 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     2701 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag ctcgaaatta
     2761 accctcacta aagggaacaa aagctggtac cgggcccccc ctcgagggca tgcgaaggaa
     2821 aatgagaaat atcgagggag acgattcaga ggagcaggac aaactataac cgactgtttg
     2881 ttggaggatg ccgtacataa cgaacactgc tgaagctacc atgtctacag tttagaggaa
     2941 tgggtacaac tcacaggcga gggatggtgt tcactcgtgc tagcaaacgc ggtgggagca
     3001 aaaagtagaa tattatcttt tattcgtgaa acttcgaaca ctgtcatcta aagatgctat
     3061 atactaatat aggcatactt gataatgaaa actataaatc gtaaagacat aagagatccg
     3121 cttatttaga gtgtcgaatt cttacgacat ttgttcattt ttgagaactc gctcaacgaa
     3181 cgatttgata tattttccca tttcatcagg tgcatcttct tgcgaaaaat gaagaccttt
     3241 tactttgaca aattcagtat taggaaactt cttggcacct tcaacaatag cattggaaaa
     3301 gaatcctggg tccgattcaa taaacatttt tggtaaatca tcacttgcac gtagataagc
     3361 attataattc ctaacaattt gtacaacgtc aggtttacca ccttttacta acgggatttc
     3421 acgaggccat gataatgttg gacgacgaac ttcacctttc tctttgaatg gttcaagata
     3481 tgctgcaaat tcttctggtt ctaactttct catgattttt gatggcaaca tggtttccac
     3541 gaagaagtta ttctccaaaa ccattttttc tccttcttca gatttgatca acgcaatatc
     3601 ttcttcaata tcaggccatt catcccatga ttcaatcaca tctactacac tttcagcgtg
     3661 aactattgct ttgatcttat cttgatgctc atagctataa tgaaatgcca aacaagcacc
     3721 ccaatcatgg ccgacaaaaa tgatcttctt tggtaaatta agaagttcaa accatgcagt
     3781 aagatatttg taatgatcaa gtaacctata agaaccatta ccagatttgc ctgatttgcc
     3841 cataccaata aggtctggta taatacaccg cgctactggc tcaatatgtg gcacaacatg
     3901 tcgccataaa taagaagagg ccgcgttacc atgtaaaaaa ataacagcat tttctgcatg
     3961 tttttctgaa tcataataat taataaatga atcaagaaca ttcatttgtt tacatctggc
     4021 ccaccactgc ggaccagtta tcatccgttt cctttgttct ggatcataaa ctttcgaagt
     4081 catcccgggg agttgattgt agcttggtat agcttgaaat attgtgcaga aaaagaaaca
     4141 aggaagaaag ggaacgagaa caatgacgag gaaacaaaag attaataatt gcaggtctat
     4201 ttatacttga tagcaagaca gcaaactttt ttttatttca aattcaagta actggaagga
     4261 aggccgtata ccgttgctca ttagagagta gtgtgcgtga atgaaggaag gaaaaagttt
     4321 cgtgtgcttc gagatacccc tcatcagctc tggaacaacg acatctgttg gtgctgtctt
     4381 tgtcgttaat tttttccttt agtgtcttcc atcatttttt tgtcattgcg gatatggtga
     4441 gacaacaacg ggggagagag aaaagaaaaa aaaagaaaag aatccgggag tttatcatta
     4501 tcaatactgc catttcaaag aatacgtaaa taattaatag tagtgatttt cctaacttta
     4561 tttagtcaaa aaattagcct tttaattctg ctgtaacccg tacatgccca aaataggggg
     4621 cgggttacac agaatatata acatcgtagg tgtctgggtg aacagtttat tcctggcatc
     4681 cactaaatat aatggagccc gctttttaag ctggcatcca gaaaaaaaaa gaatcccagc
     4741 accaaaatat tgttttcttc accaaccatc agttcatagg tccattctct tagcgcaact
     4801 acagagaaca ggggcacaaa caggcaaaaa acgggcacaa cctcaatgga gtgatgcaac
     4861 ctgcctggag taaatgatga cacaaggcaa ttgacccacg catgtatcta tctcattttc
     4921 ttacaccttc tattaccttc tgctctctct gatttggaaa aagctgaaaa aaaaggttga
     4981 aaccagttcc ctgaaattat tcccctactt gactaataag tatataaaga cggtaggtat
     5041 tgattgtaat tctgtaaatc tatttcttaa acttcttaaa ttctactttt atagttagtc
     5101 ttttttttag ttttaaaaca ccaagaactt agtttcgacg gatccatgga agacgctaag
     5161 aacattaaga agggcccagc tccattctac ccattggaag acggcactgc tggcgaacaa
     5221 ttgcacaagg ctatgaagag atacgctttg gttccaggca ctattgcttt cactgacgct
     5281 cacattgaag ttaacattac ttacgctgaa tacttcgaaa tgtctgttag attggctgaa
     5341 gctatgaaga gatacggctt gaacactaac cacagaattg ttgtttgctc tgaaaactct
     5401 ttgcaattct tcatgccagt tttgggcgct ttgttcattg gcgttgctgt tgctccagct
     5461 aacgacattt acaacgaaag agaattgttg aactctatga acatttctca accaactgtt
     5521 gttttcgttt ctaagaaggg cttgcaaaag attttgaacg ttcaaaagaa gttgccaatt
     5581 attcaaaaga ttattattat ggactctaag actgactacc aaggcttcca atctatgtac
     5641 actttcgtta cttctcactt gccaccaggc ttcaacgaat acgacttcgt tccagaatct
     5701 ttcgacagag acaagactat tgctttgatt atgaactctt ctggctctac tggcttgcca
     5761 aagggcgttg ctttgccaca cagaactgct tgcgttagat tctctcacgc tagagaccca
     5821 attttcggca accaaattat tccagacact gctattttgt ctgttgttcc attccaccac
     5881 ggcttcggca tgttcactac tttgggctac ttgatttgcg gcttcagagt tgttttgatg
     5941 tacagattcg aagaagaatt gttcttgaga tctttgcaag actacaagat tcaatctgct
     6001 ttgttggttc caactttgtt ctctttcttc gctaagtcta ctttgattga caagtacgac
     6061 ttgtctaact tgcacgaaat tgcttctggc ggcgctccat tgtctaagga agttggcgaa
     6121 gctgttgcta agagattcca cttgccaggc attagacaag gctacggctt gactgaaact
     6181 acttctgcta ttttgattac tccagaaggc gacgacaagc caggcgctgt tggcaaggtt
     6241 gttccattct tcgaagctaa ggttgttgac ttggacactg gcaagacttt gggcgttaac
     6301 caaagaggcg aattgtgcgt tagaggccca atgattatgt ctggctacgt taacaaccca
     6361 gaagctacta acgctttgat tgacaaggac ggctggttgc actctggcga cattgcttac
     6421 tgggacgaag acgaacactt cttcattgtt gacagattga agtctttgat taagtacaag
     6481 ggctaccaag ttgctccagc tgaattggaa tctattttgt tgcaacaccc aaacattttc
     6541 gacgctggcg ttgctggctt gccagacgac gacgctggcg aattgccagc tgctgttgtt
     6601 gttttggaac acggcaagac tatgactgaa aaggaaattg ttgactacgt tgcttctcaa
     6661 gttactactg ctaagaagtt gagaggcggc gttgttttcg ttgacgaagt tccaaagggc
     6721 ttgactggca agttggacgc tagaaagatt agagaaattt tgattaaggc taagaagggc
     6781 ggcaagtaag tcgacctgca ggcatgcaag ctttggactt cttcgccaga ggtttggtca
     6841 agtctccaat caaggttgtc ggcttgtcta ccttgccaga aatttacgaa aagatggaaa
     6901 agggtcaaat cgttggtaga tacgttgttg acacttctaa ataagcgaat ttcttatgat
     6961 ttatgatttt tattattaaa taagttataa aaaaaataag tgtatacaaa ttttaaagtg
     7021 actcttaggt tttaaaacga aaattcttat tcttgagtaa ctctttcctg taggtcaggt
     7081 tgctttctca ggtatagcat gaggtcgctc ttattgacca cacctctacc ggcatgccgg
     7141 tcgaaattcc cctaccctat gaaaagcggc cgccaccgcg gtggagctcc aattcgccct
     7201 atagtgagtc gtattacaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     7261 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     7321 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgga
     7381 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     7441 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     7501 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     7561 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     7621 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     7681 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     7741 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     7801 cgcgaatttt aacaaaatat taacgcttac aatttcctga tgcggtattt tctccttacg
     7861 catctgtgcg gtatttcaca ccgcataggg taataactga tataattaaa ttgaagctct
     7921 aatttgtgag tttagtatac atgcatttac ttataataca gttttttagt tttgctggcc
     7981 gcatcttctc aaatatgctt cccagcctgc ttttctgtaa cgttcaccct ctaccttagc
     8041 atcccttccc tttgcaaata gtcctcttcc aacaataata atgtcagatc ctgtagagac
     8101 cacatcatcc acggttctat actgttgacc caatgcgtct cccttgtcat ctaaacccac
     8161 accgggtgtc ataatcaacc aatcgtaacc ttcatctctt ccacccatgt ctctttgagc
     8221 aataaagccg ataacaaaat ctttgtcgct cttcgcaatg tcaacagtac ccttagtata
     8281 ttctccagta gatagggagc ccttgcatga caattctgct aacatcaaaa ggcctctagg
     8341 ttcctttgtt acttcttctg ccgcctgctt caaaccgcta acaatacctg ggcccaccac
     8401 accgtgtgca ttcgtaatgt ctgcccattc tgctattctg tatacacccg cagagtactg
     8461 caatttgact gtattaccaa tgtcagcaaa ttttctgtct tcgaagagta aaaaattgta
     8521 cttggcggat aatgccttta gcggcttaac tgtgccctcc atggaaaaat cagtcaagat
     8581 atccacatgt gtttttagta aacaaatttt gggacctaat gcttcaacta actccagtaa
     8641 ttccttggtg gtacgaacat ccaatgaagc acacaagttt gtttgctttt cgtgcatgat
     8701 attaaatagc ttggcagcaa caggactagg atgagtagca gcacgttcct tatatgtagc
     8761 tttcgacatg atttatcttc gtttcctgca ggtttttgtt ctgtgcagtt gggttaagaa
     8821 tactgggcaa tttcatgttt cttcaacact acatatgcgt atatatacca atctaagtct
     8881 gtgctccttc cttcgttctt ccttctgttc ggagattacc gaatcaaaaa aatttcaagg
     8941 aaaccgaaat caaaaaaaag aataaaaaaa aaatgatgaa ttgaaaaggt ggtatggtgc
     9001 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
     9061 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     9121 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcga
//
