LOCUS       sequence-377990-        9793 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Ti plasmid pICH86988 harbouring roGFP2-ORP1 for peroxide detection 
            in plant cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9793)
  AUTHORS   Exposito-Rodriguez M, Reeder B, Brooke GN, Hough MA, Laissue PP, 
            Mullineaux PM
  TITLE     A novel glutathione peroxidase-based biosensor disentangles 
            differential subcellular accumulation of H2O2 and lipid 
            hydroperoxides
  JOURNAL   bioRxiv 2024.01.18.576236
REFERENCE   2  (bases 1 to 9793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.01.18.576236"
COMMENT     Sequence Label: pICH-roGFP2-ORP1
FEATURES             Location/Qualifiers
     source          1..9793
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    118..142
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(265..853)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(345..364)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1024..1818)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCAKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     promoter        complement(1819..1923)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      2206..2400
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(2466..3539)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(3968..4597)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    4861..4885
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        5063..5242
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        5095..5278
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             5299..6093
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5353..5372)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5963..5982
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      6119..6371
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        7387..7732
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     7675..7695
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     terminator      join(9141..9793,1..55)
                     /label=OCS terminator
                     /note="octopine synthase terminator"
ORIGIN
        1 aaatcgttgg gcgggtccag ggcgaatttt gcgacaacat gtcgaggctc agcaggaccg
       61 ctactagagg atgcacatgt gaccgaggga cacgaagtgg tttaaactat cagtgtttga
      121 caggatatat tggcgggtaa acctaagaga aaagagcgtt tattagaata atcggatatt
      181 taaaagggcg tgaaaaggtt tatccgttcg tccatttgta tgtgccaacc acaggaaccg
      241 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
      301 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
      361 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
      421 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
      481 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
      541 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
      601 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
      661 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
      721 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
      781 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
      841 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
      901 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
      961 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     1021 cagctaaaac aattcatcca gtaaaatata atattttatt ttctcccaat caggcttgat
     1081 ccccagtaag tcaaaaaata gctcgacata ctgttcttcc ccgatatcct ccctgatcga
     1141 ccggacgcag aaggcaatgt cataccactt gtccgccctg ccgcttctcc caagatcaat
     1201 aaagccactt actttgccat ctttcacaaa gatgttgctg tctcccaggt cgccgtggga
     1261 aaagacaagt tcctcttcgg gcttttccgt ctttaaaaaa tcatacagct cgcgcggatc
     1321 tttaaatgga gtgtcctctt cccagttttc gcaatccaca tcggccagat cgttattcag
     1381 taagtaatcc aattcggcta agcggctgtc taagctattc gtatagggac aatccgatat
     1441 gtcgatggag tgaaagagcc tgatgcactc cgcatacagc tcgataatct tttcagggct
     1501 ttgttcatct tcatactctt ccgagcaaag gacgccatcg gcctcactca tgagcagatt
     1561 gctccagcca tcatgccgtt caaagtgcag gacctttgga acaggcagct ttccttccag
     1621 ccatagcatc atgtcctttt cccgttccac atcataggtg gtccctttat accggctgtc
     1681 cgtcattttt aaatataggt tttcattttc tcccaccagc ttatatacct tagcaggaga
     1741 cattccttcc gtatcttttg cgcagcggta tttttcgatc agttttttca attccggtga
     1801 tattctcatt ttagccatac tcttcctttt tcaatattat tgaagcattt atcaaggtta
     1861 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     1921 gcgcacgaat tggccagcgc tgccattttt ggggtgaggc cgttcgcggc cgaggggcgc
     1981 agcccctggg gggatgggag ctcggtgcct tgatgtgggc gccggcggtc gagtggcgac
     2041 ggcgcggctt gtccgcgccc tggtagattg cctggccgta ggccagccat ttttgagcgg
     2101 ccagcggccg cgataggccg acgcgaagcg gcggggcgta gggagcgcag cgaccgaagg
     2161 gtaggcgctt tttgcagctc ttcggctgtg cgctggccag acagttatgc acaggccagg
     2221 cgggttttaa gagttttaat aagttttaaa gagttttagg cggaaaaatc gccttttttc
     2281 tcttttatat cagtcactta catgtgtgac cggttcccaa tgtacggctt tgggttccca
     2341 atgtacgggt tccggttccc aatgtacggc tttgggttcc caatgtacgt gctatccaca
     2401 ggaaagaagc tttttcgacc tttttcccct gctagggcaa tttgccctag catctgctcc
     2461 gtacattagg aaccggcgga tgcttcgccc tcgatcaggt tgcggtagcg catgactagg
     2521 atcgggccag cctgccccgc ctcctccttc aaatcgtact ccggcaggtc atttgacccg
     2581 atcagcttgc gcacggtgaa acagaacttc ttgaactctc cggcgctgcc actgcgttcg
     2641 tagatcgtct tgaacaacca tctggcttct gccttgcctg cggcgcggcg tgccaggcgg
     2701 tagagaaaac ggccgatgcc gggatcgatc aaaaagtaat cggggtgaac cgtcagcacg
     2761 tccgggttct tgccttctgt gatctcgcgg tacatccaat cagctagctc gatctcgatg
     2821 tactccggcc gcccggtttc gctctttacg atcttgtagc ggctaatcaa ggcttcaccc
     2881 tcggataccg tcaccaggcg gccgttcttg gccttcttcg tacgctgcat ggcaacgtgc
     2941 gtggtgttta accgaatgca ggtttctacc aggtcgtctt tctgctttcc gccatcggct
     3001 cgccggcaga acttgagtac gtccgcaacg tgtggacgga acacgcggcc gggcttgtct
     3061 cccttccctt cccggtatcg gttcatggat tcggttagat gggaaaccgc catcagtacc
     3121 aggtcgtaat cccacacact ggccatgccg gccggccctg cggaaacctc tacgtgcccg
     3181 tctggaagct cgtagcggat cacctcgcca gctcgtcggt cacgcttcga cagacggaaa
     3241 acggccacgt ccatgatgct gcgactatcg cgggtgccca cgtcatagag catcggaacg
     3301 aaaaaatctg gttgctcgtc gcccttgggc ggcttcctaa tcgacgacgc accggctgcc
     3361 ggcggttgcc gggattcttt gcggattcga tcagcggccg cttgccacga ttcaccgggg
     3421 cgtgcttctg cctcgatgcg ttgccgctgg gcggcctgcg cggccttcaa cttctccacc
     3481 aggtcatcac ccagcgccgc gccgatttgt accgggccgg atggtttgcg accgctcacg
     3541 ccgattcctc gggcttgggg gttccagtgc cattgcaggg ccggcagaca acccagccgc
     3601 ttacgcctgg ccaaccgccc gttcctccac acatggggca ttccacggcg tcggtgcctg
     3661 gttgttcttg attttccatg ccgcctcctt tagccgctaa aattcatcta ctcatttatt
     3721 catttgctca tttactctgg tagctgcgcg atgtattcag atagcagctc ggtaatggtc
     3781 ttgccttggc gtaccgcgta catcttcagc ttggtgtgat cctccgccgg caactgaaag
     3841 ttgacccgct tcatggctgg cgtgtctgcc aggctggcca acgttgcagc cttgctgctg
     3901 cgtgcgctcg gacggccggc acttagcgtg tttgtgcttt tgctcatttt ctctttacct
     3961 cattaactca aatgagtttt gatttaattt cagcggccag cgcctggacc tcgcgggcag
     4021 cgtcgccctc gggttctgat tcaagaacgg ttgtgccggc ggcggcagtg cctgggtagc
     4081 tcacgcgctg cgtgatacgg gactcaagaa tgggcagctc gtacccggcc agcgcctcgg
     4141 caacctcacc gccgatgcgc gtgcctttga tcgcccgcga cacgacaaag gccgcttgta
     4201 gccttccatc cgtgacctca atgcgctgct taaccagctc caccaggtcg gcggtggccc
     4261 atatgtcgta agggcttggc tgcaccggaa tcagcacgaa gtcggctgcc ttgatcgcgg
     4321 acacagccaa gtccgccgcc tggggcgctc cgtcgatcac tacgaagtcg cgccggccga
     4381 tggccttcac gtcgcggtca atcgtcgggc ggtcgatgcc gacaacggtt agcggttgat
     4441 cttcccgcac ggccgcccaa tcgcgggcac tgccctgggg atcggaatcg actaacagaa
     4501 catcggcccc ggcgagttgc agggcgcggg ctagatgggt tgcgatggtc gtcttgcctg
     4561 acccgccttt ctggttaagt acagcgataa ccttcatgcg ttccccttgc gtatttgttt
     4621 atttactcat cgcatcatat acgcagcgac cgcatgacgc aagctgtttt actcaaatac
     4681 acatcacctt tttagacggc ggcgctcggt ttcttcagcg gccaagctgg ccggccaggc
     4741 cgccagcttg gcatcagaca aaccggccag gatttcatgc agccgcacgg ctgatgggct
     4801 gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt ggctggctgg
     4861 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
     4921 gacgttttta atgtactggg gtggatgcag tgggccccac tctgtgtgcc gaattcggat
     4981 ccggagagcg gagaattaag ggagtcacgt tatgaccccc gccgatgacg cgggacaagc
     5041 cgttttacgt ttggaactga cagaaccgca acgttgaagg agccactcag ccgcgggttt
     5101 ctggagttta atgagctaag cacatacgtc agaaaccatt attgcgcgtt caaaagtcgc
     5161 ctaaggtcac tatcagctag caaatatttc ttgtcaaaaa tgctccactg acgttccata
     5221 aattcccctc ggtatccaat tagagtctca tattcactct caatccaaat aatctgcacc
     5281 ggatctggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct
     5341 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
     5401 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
     5461 ggtgccctga atgaactcca ggacgaggca gcgcggctat cgtggctggc cacgacgggc
     5521 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
     5581 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
     5641 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
     5701 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
     5761 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
     5821 aaggcgcgta tgcccgacgg cgaggatctc gtcgtgactc atggcgatgc ctgcttgccg
     5881 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
     5941 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
     6001 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
     6061 gccttctatc gccttcttga cgagttcttc tgagcgggtc tggatctaga gtcaagcaga
     6121 tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg gtcttgcgat
     6181 gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca tgtaatgcat
     6241 gacgttattt atgagatggg tttttatgat tagagtcccg caattataca tttaatacgc
     6301 gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat
     6361 gttactagat cgacgctgca agaattcaag cttggaggaa ttccaatccc acaaaaatct
     6421 gagcttaaca gcacagttgc tcctctcaga gcagaatcgg gtattcaaca ccctcatatc
     6481 aactactacg ttgtgtataa cggtccacat gccggtatat acgatgactg gggttgtaca
     6541 aaggcggcaa caaacggcgt tcccggagtt gcacacaaga aatttgccac tattacagag
     6601 gcaagagcag cagctgacgc gtacacaaca agtcagcaaa cagacaggtt gaacttcatc
     6661 cccaaaggag aagctcaact caagcccaag agctttgcta aggccctaac aagcccacca
     6721 aagcaaaaag cccactggct cacgctagga accaaaaggc ccagcagtga tccagcccca
     6781 aaagagatct cctttgcccc ggagattaca atggacgatt tcctctatct ttacgatcta
     6841 ggaaggaagt tcgaaggtga aggtgacgac actatgttca ccactgataa tgagaaggtt
     6901 agcctcttca atttcagaaa gaatgctgac ccacagatgg ttagagaggc ctacgcagca
     6961 agtctcatca agacgatcta cccgagtaac aatctccagg agatcaaata ccttcccaag
     7021 aaggttaaag atgcagtcaa aagattcagg actaattgca tcaagaacac agagaaagac
     7081 atatttctca agatcagaag tactattcca gtatggacga ttcaaggctt gcttcataaa
     7141 ccaaggcaag taatagagat tggagtctct aaaaaggtag ttcctactga atctaaggcc
     7201 atgcatggag tctaagattc aaatcgagga tctaacagaa ctcgccgtca agactggcga
     7261 acagttcata cagagtcttt tacgactcaa tgacaagaag aaaatcttcg tcaacatggt
     7321 ggagcacgac actctggtct actccaaaaa tgtcaaagat acagtctcag aagatcaaag
     7381 ggctattgag acttttcaac aaaggataat ttcgggaaac ctcctcggat tccattgccc
     7441 agctatctgt cacttcatcg aaaggacagt agaaaaggaa ggtggctcct acaaatgcca
     7501 tcattgcgat aaaggaaagg ctatcattca agatctctct gccgacagtg gtcccaaaga
     7561 tggaccccca cccacgagga gcatcgtgga aaaagaagag gttccaacca cgtctacaaa
     7621 gcaagtggat tgatgtgaca tctccactga cgtaagggat gacgcacaat cccactatcc
     7681 ttcgcaagac ccttcctcta tataaggaag ttcatttcat ttggagagga cacgctcgag
     7741 tataagagct catttttaca acaattacca acaacaacaa acaacaaaca acattacaat
     7801 tacatttaca attatcgata caatggttag caaaggtgaa gaactgttca ccggtgttgt
     7861 gccgatcctg gttgaactgg acggtgacgt taacggtcac aaattctctg tttccggtga
     7921 aggtgaaggt gacgctacct acggtaaact gaccctgaaa ttcatctcta ccaccggtaa
     7981 actgccggtt ccgtggccga ccctggtgac caccctgacc tacggtgttc agtgcttctc
     8041 ccgttacccg gaccacatga aacagcacga tttcttcaaa tccgcgatgc cggaaggtta
     8101 cgtgcaggaa cgtaccatct tcttcaaaga tgacggtaac tacaaaaccc gtgctgaagt
     8161 taaattcgaa ggtgacaccc tggttaaccg tattgaactg aaaggcatcg acttcaaaga
     8221 agatggtaac atcctgggtc acaaactgga atacaactac aactgtcaca acgtttacat
     8281 catggcggat aaacagaaaa acggtatcaa agttaacttc aaaatccgtc acaacatcga
     8341 agatggttct gttcagctgg ctgatcacta ccagcagaac accccgatcg gtgacggtcc
     8401 ggttctgctg ccggacaacc actacctgtc tacctgctcc gcgctgagca aagacccgaa
     8461 cgaaaaacgt gaccacatgg ttctgctgga attcgttacc gcggcgggta tcaccctggg
     8521 catggatgaa ctgtacaaaa cctccggcgg ttctggtggc ggtggctctg gtggtggtgg
     8581 ttctggtggt ggcggctctg gtggtggtgg ttctggtggc ggtggttctg gcggcgaatt
     8641 cgacatcagc gaattctaca aactggctcc ggttgacaaa aaaggtcagc cgttcccgtt
     8701 cgaccagctg aaaggtaaag tggtgctgat cgttaacgtt gcatctaaat gcggtttcac
     8761 cccgcagtac aaagaactgg aagcgctgta taaacgttac aaagatgaag gtttcactat
     8821 catcggcttc ccgtgtaacc agttcggtca ccaggaaccg ggctctgatg aagaaatcgc
     8881 ccagttctgc caactgaact atggcgtgac tttcccgatc atgaaaaaga tcgacgttaa
     8941 cggtggtaac gaagatccgg tttataaatt cctgaagagc caaaaatccg gtatgttggg
     9001 cttgagaggt atcaaatgga acttcgaaaa attcctggtt gacaaaaaag gtaaagttta
     9061 cgaacgttat agctctctga ccaaaccgtc ctctctgtct gaaaccatcg aagaactgct
     9121 gaaagaagtt taagcttgtc ctgctttaat gagatatgcg agaagcctat gatcgcatga
     9181 tatttgcttt caattctgtt gtgcacgttg taaaaaacct gagcatgtgt agctcagatc
     9241 cttaccgccg gtttcggttc attctaatga atatatcacc cgttactatc gtatttttat
     9301 gaataatatt ctccgttcaa tttactgatt gtaccctact acttatatgt acaatattaa
     9361 aatgaaaaca atatattgtg ctgaataggt ttatagcgac atctatgata gagcgccaca
     9421 ataacaaaca attgcgtttt attattacaa atccaatttt aaaaaaagcg gcagaaccgg
     9481 tcaaacctaa aagactgatt acataaatct tattcaaatt tcaaaagtgc cccaggggct
     9541 agtatctacg acacaccgag cggcgaacta ataacgctca ctgaagggaa ctccggttcc
     9601 ccgccggcgc gcatgggtga gattccttga agttgagtat tggccgtccg ctctaccgaa
     9661 agttacgggc accattcaac ccggtccagc acggcggccg ggtaaccgac ttgctgcccc
     9721 gagaattatg cagcattttt ttggtgtatg tgggccccaa atgaagtgca ggtcaaacct
     9781 tgacagtgac gac
//
