LOCUS       sequence-377992-        9865 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Ti plasmid pICH86988 harbouring roGFP2-synORP1 for H2O2 detection in
            plant cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9865)
  AUTHORS   Exposito-Rodriguez M, Reeder B, Brooke GN, Hough MA, Laissue PP, 
            Mullineaux PM
  TITLE     A novel glutathione peroxidase-based biosensor disentangles 
            differential subcellular accumulation of H2O2 and lipid 
            hydroperoxides
  JOURNAL   bioRxiv 2024.01.18.576236
REFERENCE   2  (bases 1 to 9865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.01.18.576236"
COMMENT     Sequence Label: pICH-roGFP2-synORP1
FEATURES             Location/Qualifiers
     source          1..9865
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      700..1407
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     misc_feature    1470..1494
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(1617..2205)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1697..1716)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2376..3170)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCAKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     promoter        complement(3171..3275)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3558..3752
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(3818..4891)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(5320..5949)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    6213..6237
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        6415..6594
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        6447..6630
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             6651..7445
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6705..6724)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7315..7334
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      7471..7723
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        8739..9084
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     9027..9047
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 ccctgggcat ggatgaactg tacaaaacct ccggcggttc tggtggcggt ggctctggtg
       61 gtggtggttc tggtggtggc ggctctggtg gtggtggttc tggtggcggt ggttctggcg
      121 gcgaattcga catcagcgaa ttctacaaac tggctccggt tgacaaaaaa ggtcagccgt
      181 tcccgttcga ccagctgaaa ggtaaagtgg tgctgatcgt taacgttgca tctaaatgcg
      241 gtttcacccc gcagtacaaa gaactggaag cgctgtataa acgttacaaa gatgaaggtt
      301 tcactatcat cggcttcccg tgtaaccagt tcggtcacca ggaaccgggc tctgatgaag
      361 aaatcctgaa ctctctgaaa tacgttcgtc cgggtggtgg tttcgaaccg aacttcccga
      421 tcatgaaaaa gatcgacgtt aacggtggta acgaagatcc ggtttataaa ttcctgcgtg
      481 aagcgctgcc ggctccgtct gatgacgcga ccgcgctgat gaccgatccg aaactgatca
      541 cctggagccc ggtttgccgt aacgatgttg cgtggaactt cgaaaaattc ctggttgaca
      601 aaaaaggtaa agtttacgaa cgttatagct ctctgaccaa accgtcctct ctgtctgaaa
      661 ccatcgaaga actgctgaaa gaagttgaat aagcttgtcc tgctttaatg agatatgcga
      721 gaagcctatg atcgcatgat atttgctttc aattctgttg tgcacgttgt aaaaaacctg
      781 agcatgtgta gctcagatcc ttaccgccgg tttcggttca ttctaatgaa tatatcaccc
      841 gttactatcg tatttttatg aataatattc tccgttcaat ttactgattg taccctacta
      901 cttatatgta caatattaaa atgaaaacaa tatattgtgc tgaataggtt tatagcgaca
      961 tctatgatag agcgccacaa taacaaacaa ttgcgtttta ttattacaaa tccaatttta
     1021 aaaaaagcgg cagaaccggt caaacctaaa agactgatta cataaatctt attcaaattt
     1081 caaaagtgcc ccaggggcta gtatctacga cacaccgagc ggcgaactaa taacgctcac
     1141 tgaagggaac tccggttccc cgccggcgcg catgggtgag attccttgaa gttgagtatt
     1201 ggccgtccgc tctaccgaaa gttacgggca ccattcaacc cggtccagca cggcggccgg
     1261 gtaaccgact tgctgccccg agaattatgc agcatttttt tggtgtatgt gggccccaaa
     1321 tgaagtgcag gtcaaacctt gacagtgacg acaaatcgtt gggcgggtcc agggcgaatt
     1381 ttgcgacaac atgtcgaggc tcagcaggac cgctactaga ggatgcacat gtgaccgagg
     1441 gacacgaagt ggtttaaact atcagtgttt gacaggatat attggcgggt aaacctaaga
     1501 gaaaagagcg tttattagaa taatcggata tttaaaaggg cgtgaaaagg tttatccgtt
     1561 cgtccatttg tatgtgccaa ccacaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     1621 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     1681 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     1741 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     1801 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     1861 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     1921 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     1981 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     2041 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     2101 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     2161 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     2221 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     2281 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     2341 atctaaagta tatatgagta aacttggtct gacagctaaa acaattcatc cagtaaaata
     2401 taatatttta ttttctccca atcaggcttg atccccagta agtcaaaaaa tagctcgaca
     2461 tactgttctt ccccgatatc ctccctgatc gaccggacgc agaaggcaat gtcataccac
     2521 ttgtccgccc tgccgcttct cccaagatca ataaagccac ttactttgcc atctttcaca
     2581 aagatgttgc tgtctcccag gtcgccgtgg gaaaagacaa gttcctcttc gggcttttcc
     2641 gtctttaaaa aatcatacag ctcgcgcgga tctttaaatg gagtgtcctc ttcccagttt
     2701 tcgcaatcca catcggccag atcgttattc agtaagtaat ccaattcggc taagcggctg
     2761 tctaagctat tcgtataggg acaatccgat atgtcgatgg agtgaaagag cctgatgcac
     2821 tccgcataca gctcgataat cttttcaggg ctttgttcat cttcatactc ttccgagcaa
     2881 aggacgccat cggcctcact catgagcaga ttgctccagc catcatgccg ttcaaagtgc
     2941 aggacctttg gaacaggcag ctttccttcc agccatagca tcatgtcctt ttcccgttcc
     3001 acatcatagg tggtcccttt ataccggctg tccgtcattt ttaaatatag gttttcattt
     3061 tctcccacca gcttatatac cttagcagga gacattcctt ccgtatcttt tgcgcagcgg
     3121 tatttttcga tcagtttttt caattccggt gatattctca ttttagccat actcttcctt
     3181 tttcaatatt attgaagcat ttatcaaggt tattgtctca tgagcggata catatttgaa
     3241 tgtatttaga aaaataaaca aataggggtt ccgcgcacga attggccagc gctgccattt
     3301 ttggggtgag gccgttcgcg gccgaggggc gcagcccctg gggggatggg agctcggtgc
     3361 cttgatgtgg gcgccggcgg tcgagtggcg acggcgcggc ttgtccgcgc cctggtagat
     3421 tgcctggccg taggccagcc atttttgagc ggccagcggc cgcgataggc cgacgcgaag
     3481 cggcggggcg tagggagcgc agcgaccgaa gggtaggcgc tttttgcagc tcttcggctg
     3541 tgcgctggcc agacagttat gcacaggcca ggcgggtttt aagagtttta ataagtttta
     3601 aagagtttta ggcggaaaaa tcgccttttt tctcttttat atcagtcact tacatgtgtg
     3661 accggttccc aatgtacggc tttgggttcc caatgtacgg gttccggttc ccaatgtacg
     3721 gctttgggtt cccaatgtac gtgctatcca caggaaagaa gctttttcga cctttttccc
     3781 ctgctagggc aatttgccct agcatctgct ccgtacatta ggaaccggcg gatgcttcgc
     3841 cctcgatcag gttgcggtag cgcatgacta ggatcgggcc agcctgcccc gcctcctcct
     3901 tcaaatcgta ctccggcagg tcatttgacc cgatcagctt gcgcacggtg aaacagaact
     3961 tcttgaactc tccggcgctg ccactgcgtt cgtagatcgt cttgaacaac catctggctt
     4021 ctgccttgcc tgcggcgcgg cgtgccaggc ggtagagaaa acggccgatg ccgggatcga
     4081 tcaaaaagta atcggggtga accgtcagca cgtccgggtt cttgccttct gtgatctcgc
     4141 ggtacatcca atcagctagc tcgatctcga tgtactccgg ccgcccggtt tcgctcttta
     4201 cgatcttgta gcggctaatc aaggcttcac cctcggatac cgtcaccagg cggccgttct
     4261 tggccttctt cgtacgctgc atggcaacgt gcgtggtgtt taaccgaatg caggtttcta
     4321 ccaggtcgtc tttctgcttt ccgccatcgg ctcgccggca gaacttgagt acgtccgcaa
     4381 cgtgtggacg gaacacgcgg ccgggcttgt ctcccttccc ttcccggtat cggttcatgg
     4441 attcggttag atgggaaacc gccatcagta ccaggtcgta atcccacaca ctggccatgc
     4501 cggccggccc tgcggaaacc tctacgtgcc cgtctggaag ctcgtagcgg atcacctcgc
     4561 cagctcgtcg gtcacgcttc gacagacgga aaacggccac gtccatgatg ctgcgactat
     4621 cgcgggtgcc cacgtcatag agcatcggaa cgaaaaaatc tggttgctcg tcgcccttgg
     4681 gcggcttcct aatcgacgac gcaccggctg ccggcggttg ccgggattct ttgcggattc
     4741 gatcagcggc cgcttgccac gattcaccgg ggcgtgcttc tgcctcgatg cgttgccgct
     4801 gggcggcctg cgcggccttc aacttctcca ccaggtcatc acccagcgcc gcgccgattt
     4861 gtaccgggcc ggatggtttg cgaccgctca cgccgattcc tcgggcttgg gggttccagt
     4921 gccattgcag ggccggcaga caacccagcc gcttacgcct ggccaaccgc ccgttcctcc
     4981 acacatgggg cattccacgg cgtcggtgcc tggttgttct tgattttcca tgccgcctcc
     5041 tttagccgct aaaattcatc tactcattta ttcatttgct catttactct ggtagctgcg
     5101 cgatgtattc agatagcagc tcggtaatgg tcttgccttg gcgtaccgcg tacatcttca
     5161 gcttggtgtg atcctccgcc ggcaactgaa agttgacccg cttcatggct ggcgtgtctg
     5221 ccaggctggc caacgttgca gccttgctgc tgcgtgcgct cggacggccg gcacttagcg
     5281 tgtttgtgct tttgctcatt ttctctttac ctcattaact caaatgagtt ttgatttaat
     5341 ttcagcggcc agcgcctgga cctcgcgggc agcgtcgccc tcgggttctg attcaagaac
     5401 ggttgtgccg gcggcggcag tgcctgggta gctcacgcgc tgcgtgatac gggactcaag
     5461 aatgggcagc tcgtacccgg ccagcgcctc ggcaacctca ccgccgatgc gcgtgccttt
     5521 gatcgcccgc gacacgacaa aggccgcttg tagccttcca tccgtgacct caatgcgctg
     5581 cttaaccagc tccaccaggt cggcggtggc ccatatgtcg taagggcttg gctgcaccgg
     5641 aatcagcacg aagtcggctg ccttgatcgc ggacacagcc aagtccgccg cctggggcgc
     5701 tccgtcgatc actacgaagt cgcgccggcc gatggccttc acgtcgcggt caatcgtcgg
     5761 gcggtcgatg ccgacaacgg ttagcggttg atcttcccgc acggccgccc aatcgcgggc
     5821 actgccctgg ggatcggaat cgactaacag aacatcggcc ccggcgagtt gcagggcgcg
     5881 ggctagatgg gttgcgatgg tcgtcttgcc tgacccgcct ttctggttaa gtacagcgat
     5941 aaccttcatg cgttcccctt gcgtatttgt ttatttactc atcgcatcat atacgcagcg
     6001 accgcatgac gcaagctgtt ttactcaaat acacatcacc tttttagacg gcggcgctcg
     6061 gtttcttcag cggccaagct ggccggccag gccgccagct tggcatcaga caaaccggcc
     6121 aggatttcat gcagccgcac ggctgatggg ctgcctgtat cgagtggtga ttttgtgccg
     6181 agctgccggt cggggagctg ttggctggct ggtggcagga tatattgtgg tgtaaacaaa
     6241 ttgacgctta gacaacttaa taacacattg cggacgtttt taatgtactg gggtggatgc
     6301 agtgggcccc actctgtgtg ccgaattcgg atccggagag cggagaatta agggagtcac
     6361 gttatgaccc ccgccgatga cgcgggacaa gccgttttac gtttggaact gacagaaccg
     6421 caacgttgaa ggagccactc agccgcgggt ttctggagtt taatgagcta agcacatacg
     6481 tcagaaacca ttattgcgcg ttcaaaagtc gcctaaggtc actatcagct agcaaatatt
     6541 tcttgtcaaa aatgctccac tgacgttcca taaattcccc tcggtatcca attagagtct
     6601 catattcact ctcaatccaa ataatctgca ccggatctgg atcgtttcgc atgattgaac
     6661 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     6721 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     6781 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactc caggacgagg
     6841 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     6901 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     6961 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     7021 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     7081 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     7141 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg tatgcccgac ggcgaggatc
     7201 tcgtcgtgac tcatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     7261 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     7321 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     7381 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     7441 tctgagcggg tctggatcta gagtcaagca gatcgttcaa acatttggca ataaagtttc
     7501 ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac
     7561 gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg
     7621 attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac
     7681 taggataaat tatcgcgcgc ggtgtcatct atgttactag atcgacgctg caagaattca
     7741 agcttggagg aattccaatc ccacaaaaat ctgagcttaa cagcacagtt gctcctctca
     7801 gagcagaatc gggtattcaa caccctcata tcaactacta cgttgtgtat aacggtccac
     7861 atgccggtat atacgatgac tggggttgta caaaggcggc aacaaacggc gttcccggag
     7921 ttgcacacaa gaaatttgcc actattacag aggcaagagc agcagctgac gcgtacacaa
     7981 caagtcagca aacagacagg ttgaacttca tccccaaagg agaagctcaa ctcaagccca
     8041 agagctttgc taaggcccta acaagcccac caaagcaaaa agcccactgg ctcacgctag
     8101 gaaccaaaag gcccagcagt gatccagccc caaaagagat ctcctttgcc ccggagatta
     8161 caatggacga tttcctctat ctttacgatc taggaaggaa gttcgaaggt gaaggtgacg
     8221 acactatgtt caccactgat aatgagaagg ttagcctctt caatttcaga aagaatgctg
     8281 acccacagat ggttagagag gcctacgcag caagtctcat caagacgatc tacccgagta
     8341 acaatctcca ggagatcaaa taccttccca agaaggttaa agatgcagtc aaaagattca
     8401 ggactaattg catcaagaac acagagaaag acatatttct caagatcaga agtactattc
     8461 cagtatggac gattcaaggc ttgcttcata aaccaaggca agtaatagag attggagtct
     8521 ctaaaaaggt agttcctact gaatctaagg ccatgcatgg agtctaagat tcaaatcgag
     8581 gatctaacag aactcgccgt caagactggc gaacagttca tacagagtct tttacgactc
     8641 aatgacaaga agaaaatctt cgtcaacatg gtggagcacg acactctggt ctactccaaa
     8701 aatgtcaaag atacagtctc agaagatcaa agggctattg agacttttca acaaaggata
     8761 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     8821 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     8881 caagatctct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     8941 gaaaaagaag aggttccaac cacgtctaca aagcaagtgg attgatgtga catctccact
     9001 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     9061 agttcatttc atttggagag gacacgctcg agtataagag ctcattttta caacaattac
     9121 caacaacaac aaacaacaaa caacattaca attacattta caattatcga tacaatggtt
     9181 agcaaaggtg aagaactgtt caccggtgtt gtgccgatcc tggttgaact ggacggtgac
     9241 gttaacggtc acaaattctc tgtttccggt gaaggtgaag gtgacgctac ctacggtaaa
     9301 ctgaccctga aattcatctc taccaccggt aaactgccgg ttccgtggcc gaccctggtg
     9361 accaccctga cctacggtgt tcagtgcttc tcccgttacc cggaccacat gaaacagcac
     9421 gatttcttca aatccgcgat gccggaaggt tacgtgcagg aacgtaccat cttcttcaaa
     9481 gatgacggta actacaaaac ccgtgctgaa gttaaattcg aaggtgacac cctggttaac
     9541 cgtattgaac tgaaaggcat cgacttcaaa gaagatggta acatcctggg tcacaaactg
     9601 gaatacaact acaactgtca caacgtttac atcatggcgg ataaacagaa aaacggtatc
     9661 aaagttaact tcaaaatccg tcacaacatc gaagatggtt ctgttcagct ggctgatcac
     9721 taccagcaga acaccccgat cggtgacggt ccggttctgc tgccggacaa ccactacctg
     9781 tctacctgct ccgcgctgag caaagacccg aacgaaaaac gtgaccacat ggttctgctg
     9841 gaattcgtta ccgcggcggg tatca
//
