LOCUS       sequence-377993-        9802 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Ti plasmid pICH86988 harbouring roGFP2-GPXC71S -a control roGFP2 
            fusion not responsive to peroxides used alongside plasmids 191680 
            and 191677.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9802)
  AUTHORS   Exposito-Rodriguez M, Reeder B, Brooke GN, Hough MA, Laissue PP, 
            Mullineaux PM
  TITLE     A novel glutathione peroxidase-based biosensor disentangles 
            differential subcellular accumulation of H2O2 and lipid 
            hydroperoxides
  JOURNAL   bioRxiv 2024.01.18.576236
REFERENCE   2  (bases 1 to 9802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.01.18.576236"
COMMENT     Sequence Label: pICH-roGFP2-GPXC71S
FEATURES             Location/Qualifiers
     source          1..9802
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(257..886)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    1150..1174
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        1352..1531
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        1384..1567
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             1588..2382
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1642..1661)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2252..2271
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      2408..2660
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        3676..4021
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     3964..3984
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     terminator      5439..6146
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     misc_feature    6209..6233
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(6356..6944)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6436..6455)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7115..7909)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCAKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     promoter        complement(7910..8014)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8297..8491
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8557..9630)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
ORIGIN
        1 tcatttattc atttgctcat ttactctggt agctgcgcga tgtattcaga tagcagctcg
       61 gtaatggtct tgccttggcg taccgcgtac atcttcagct tggtgtgatc ctccgccggc
      121 aactgaaagt tgacccgctt catggctggc gtgtctgcca ggctggccaa cgttgcagcc
      181 ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt ttgtgctttt gctcattttc
      241 tctttacctc attaactcaa atgagttttg atttaatttc agcggccagc gcctggacct
      301 cgcgggcagc gtcgccctcg ggttctgatt caagaacggt tgtgccggcg gcggcagtgc
      361 ctgggtagct cacgcgctgc gtgatacggg actcaagaat gggcagctcg tacccggcca
      421 gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat cgcccgcgac acgacaaagg
      481 ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt aaccagctcc accaggtcgg
      541 cggtggccca tatgtcgtaa gggcttggct gcaccggaat cagcacgaag tcggctgcct
      601 tgatcgcgga cacagccaag tccgccgcct ggggcgctcc gtcgatcact acgaagtcgc
      661 gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg gtcgatgccg acaacggtta
      721 gcggttgatc ttcccgcacg gccgcccaat cgcgggcact gccctgggga tcggaatcga
      781 ctaacagaac atcggccccg gcgagttgca gggcgcgggc tagatgggtt gcgatggtcg
      841 tcttgcctga cccgcctttc tggttaagta cagcgataac cttcatgcgt tccccttgcg
      901 tatttgttta tttactcatc gcatcatata cgcagcgacc gcatgacgca agctgtttta
      961 ctcaaataca catcaccttt ttagacggcg gcgctcggtt tcttcagcgg ccaagctggc
     1021 cggccaggcc gccagcttgg catcagacaa accggccagg atttcatgca gccgcacggc
     1081 tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg
     1141 gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa
     1201 cacattgcgg acgtttttaa tgtactgggg tggatgcagt gggccccact ctgtgtgccg
     1261 aattcggatc cggagagcgg agaattaagg gagtcacgtt atgacccccg ccgatgacgc
     1321 gggacaagcc gttttacgtt tggaactgac agaaccgcaa cgttgaagga gccactcagc
     1381 cgcgggtttc tggagtttaa tgagctaagc acatacgtca gaaaccatta ttgcgcgttc
     1441 aaaagtcgcc taaggtcact atcagctagc aaatatttct tgtcaaaaat gctccactga
     1501 cgttccataa attcccctcg gtatccaatt agagtctcat attcactctc aatccaaata
     1561 atctgcaccg gatctggatc gtttcgcatg attgaacaag atggattgca cgcaggttct
     1621 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc
     1681 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc
     1741 gacctgtccg gtgccctgaa tgaactccag gacgaggcag cgcggctatc gtggctggcc
     1801 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg
     1861 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag
     1921 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc
     1981 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt
     2041 cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
     2101 gccaggctca aggcgcgtat gcccgacggc gaggatctcg tcgtgactca tggcgatgcc
     2161 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg
     2221 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag
     2281 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg
     2341 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggtct ggatctagag
     2401 tcaagcagat cgttcaaaca tttggcaata aagtttctta agattgaatc ctgttgccgg
     2461 tcttgcgatg attatcatat aatttctgtt gaattacgtt aagcatgtaa taattaacat
     2521 gtaatgcatg acgttattta tgagatgggt ttttatgatt agagtcccgc aattatacat
     2581 ttaatacgcg atagaaaaca aaatatagcg cgcaaactag gataaattat cgcgcgcggt
     2641 gtcatctatg ttactagatc gacgctgcaa gaattcaagc ttggaggaat tccaatccca
     2701 caaaaatctg agcttaacag cacagttgct cctctcagag cagaatcggg tattcaacac
     2761 cctcatatca actactacgt tgtgtataac ggtccacatg ccggtatata cgatgactgg
     2821 ggttgtacaa aggcggcaac aaacggcgtt cccggagttg cacacaagaa atttgccact
     2881 attacagagg caagagcagc agctgacgcg tacacaacaa gtcagcaaac agacaggttg
     2941 aacttcatcc ccaaaggaga agctcaactc aagcccaaga gctttgctaa ggccctaaca
     3001 agcccaccaa agcaaaaagc ccactggctc acgctaggaa ccaaaaggcc cagcagtgat
     3061 ccagccccaa aagagatctc ctttgccccg gagattacaa tggacgattt cctctatctt
     3121 tacgatctag gaaggaagtt cgaaggtgaa ggtgacgaca ctatgttcac cactgataat
     3181 gagaaggtta gcctcttcaa tttcagaaag aatgctgacc cacagatggt tagagaggcc
     3241 tacgcagcaa gtctcatcaa gacgatctac ccgagtaaca atctccagga gatcaaatac
     3301 cttcccaaga aggttaaaga tgcagtcaaa agattcagga ctaattgcat caagaacaca
     3361 gagaaagaca tatttctcaa gatcagaagt actattccag tatggacgat tcaaggcttg
     3421 cttcataaac caaggcaagt aatagagatt ggagtctcta aaaaggtagt tcctactgaa
     3481 tctaaggcca tgcatggagt ctaagattca aatcgaggat ctaacagaac tcgccgtcaa
     3541 gactggcgaa cagttcatac agagtctttt acgactcaat gacaagaaga aaatcttcgt
     3601 caacatggtg gagcacgaca ctctggtcta ctccaaaaat gtcaaagata cagtctcaga
     3661 agatcaaagg gctattgaga cttttcaaca aaggataatt tcgggaaacc tcctcggatt
     3721 ccattgccca gctatctgtc acttcatcga aaggacagta gaaaaggaag gtggctccta
     3781 caaatgccat cattgcgata aaggaaaggc tatcattcaa gatctctctg ccgacagtgg
     3841 tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagagg ttccaaccac
     3901 gtctacaaag caagtggatt gatgtgacat ctccactgac gtaagggatg acgcacaatc
     3961 ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggac
     4021 acgctcgagt ataagagctc atttttacaa caattaccaa caacaacaaa caacaaacaa
     4081 cattacaatt acatttacaa ttatcgatac aatggtttct aaaggtgaag aactgttcac
     4141 cggtgttgtt ccgatcctgg ttgaactgga tggtgacgtt aacggtcaca aattctctgt
     4201 ttctggtgaa ggtgaaggtg atgctaccta cggtaaactg accctgaaat tcatctccac
     4261 cactggtaaa ctgccggtgc cgtggccgac cctggttacc accctgacct acggcgttca
     4321 gtgcttctct cgttacccgg accacatgaa acagcacgac ttcttcaaat ccgctatgcc
     4381 ggaaggctac gttcaggaac gcaccatttt cttcaaagat gacggtaact acaaaacccg
     4441 cgctgaagtt aaattcgaag gtgatactct ggttaaccgt atcgaactga aaggtatcga
     4501 tttcaaagaa gacggtaaca tcctgggtca caaactggaa tacaactaca actgccacaa
     4561 cgtgtacatc atggcggaca aacagaaaaa cggtattaaa gttaacttca aaatccgtca
     4621 caacatcgaa gatggcagcg tgcagctggc cgaccactac cagcagaaca ccccgatcgg
     4681 tgacggtccg gttctgctgc cggacaacca ctacctgtcc acctgctccg cgctgtctaa
     4741 agacccgaac gaaaaacgtg atcacatggt tctgctggaa ttcgttaccg cagcgggcat
     4801 caccctgggt atggacgaac tgtacaaaac ttctggtggt tctggcggtg gcggctctgg
     4861 cggtggtggt agcggcggcg gtggttctgg tggcggtggt tccggtggcg gcggtagcgg
     4921 tggcgaattc gaccagtctt cttactcttc catctaccac atctctgtta aagacatcga
     4981 tggcaacgac gtgtctctgt ctaaattcac cggtaaagtg ctgctgatcg taaacgttgc
     5041 gtctaaatcc ggtctgaccc agggtaacta caaagaactg aacatcctgt acgcgaaata
     5101 caaaaccaaa ggtctggaaa tcctggcgtt cccgtgtaac cagttcggtt ctcaggaacc
     5161 gggttctaac aaagaaatta aagataacat ctgtaccacc tttaaaggtg aattcccgat
     5221 ctttgataaa attgaggtta acggtgaaaa cgcgtcccca ctgtataaat ttctgaaaga
     5281 gcagaaaggc ggtctgttcg gtgatagcat taaatggaac tttgctaaat tcttagttga
     5341 taaacaaggt aacgtagttg atcgtttcgc gccgaccact agcccgttag agattgagaa
     5401 agacattgaa aaactgctgg catctaccta agcttgtcct gctttaatga gatatgcgag
     5461 aagcctatga tcgcatgata tttgctttca attctgttgt gcacgttgta aaaaacctga
     5521 gcatgtgtag ctcagatcct taccgccggt ttcggttcat tctaatgaat atatcacccg
     5581 ttactatcgt atttttatga ataatattct ccgttcaatt tactgattgt accctactac
     5641 ttatatgtac aatattaaaa tgaaaacaat atattgtgct gaataggttt atagcgacat
     5701 ctatgataga gcgccacaat aacaaacaat tgcgttttat tattacaaat ccaattttaa
     5761 aaaaagcggc agaaccggtc aaacctaaaa gactgattac ataaatctta ttcaaatttc
     5821 aaaagtgccc caggggctag tatctacgac acaccgagcg gcgaactaat aacgctcact
     5881 gaagggaact ccggttcccc gccggcgcgc atgggtgaga ttccttgaag ttgagtattg
     5941 gccgtccgct ctaccgaaag ttacgggcac cattcaaccc ggtccagcac ggcggccggg
     6001 taaccgactt gctgccccga gaattatgca gcattttttt ggtgtatgtg ggccccaaat
     6061 gaagtgcagg tcaaaccttg acagtgacga caaatcgttg ggcgggtcca gggcgaattt
     6121 tgcgacaaca tgtcgaggct cagcaggacc gctactagag gatgcacatg tgaccgaggg
     6181 acacgaagtg gtttaaacta tcagtgtttg acaggatata ttggcgggta aacctaagag
     6241 aaaagagcgt ttattagaat aatcggatat ttaaaagggc gtgaaaaggt ttatccgttc
     6301 gtccatttgt atgtgccaac cacaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     6361 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     6421 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     6481 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     6541 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     6601 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     6661 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     6721 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     6781 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     6841 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     6901 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     6961 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     7021 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     7081 tctaaagtat atatgagtaa acttggtctg acagctaaaa caattcatcc agtaaaatat
     7141 aatattttat tttctcccaa tcaggcttga tccccagtaa gtcaaaaaat agctcgacat
     7201 actgttcttc cccgatatcc tccctgatcg accggacgca gaaggcaatg tcataccact
     7261 tgtccgccct gccgcttctc ccaagatcaa taaagccact tactttgcca tctttcacaa
     7321 agatgttgct gtctcccagg tcgccgtggg aaaagacaag ttcctcttcg ggcttttccg
     7381 tctttaaaaa atcatacagc tcgcgcggat ctttaaatgg agtgtcctct tcccagtttt
     7441 cgcaatccac atcggccaga tcgttattca gtaagtaatc caattcggct aagcggctgt
     7501 ctaagctatt cgtataggga caatccgata tgtcgatgga gtgaaagagc ctgatgcact
     7561 ccgcatacag ctcgataatc ttttcagggc tttgttcatc ttcatactct tccgagcaaa
     7621 ggacgccatc ggcctcactc atgagcagat tgctccagcc atcatgccgt tcaaagtgca
     7681 ggacctttgg aacaggcagc tttccttcca gccatagcat catgtccttt tcccgttcca
     7741 catcataggt ggtcccttta taccggctgt ccgtcatttt taaatatagg ttttcatttt
     7801 ctcccaccag cttatatacc ttagcaggag acattccttc cgtatctttt gcgcagcggt
     7861 atttttcgat cagttttttc aattccggtg atattctcat tttagccata ctcttccttt
     7921 ttcaatatta ttgaagcatt tatcaaggtt attgtctcat gagcggatac atatttgaat
     7981 gtatttagaa aaataaacaa ataggggttc cgcgcacgaa ttggccagcg ctgccatttt
     8041 tggggtgagg ccgttcgcgg ccgaggggcg cagcccctgg ggggatggga gctcggtgcc
     8101 ttgatgtggg cgccggcggt cgagtggcga cggcgcggct tgtccgcgcc ctggtagatt
     8161 gcctggccgt aggccagcca tttttgagcg gccagcggcc gcgataggcc gacgcgaagc
     8221 ggcggggcgt agggagcgca gcgaccgaag ggtaggcgct ttttgcagct cttcggctgt
     8281 gcgctggcca gacagttatg cacaggccag gcgggtttta agagttttaa taagttttaa
     8341 agagttttag gcggaaaaat cgcctttttt ctcttttata tcagtcactt acatgtgtga
     8401 ccggttccca atgtacggct ttgggttccc aatgtacggg ttccggttcc caatgtacgg
     8461 ctttgggttc ccaatgtacg tgctatccac aggaaagaag ctttttcgac ctttttcccc
     8521 tgctagggca atttgcccta gcatctgctc cgtacattag gaaccggcgg atgcttcgcc
     8581 ctcgatcagg ttgcggtagc gcatgactag gatcgggcca gcctgccccg cctcctcctt
     8641 caaatcgtac tccggcaggt catttgaccc gatcagcttg cgcacggtga aacagaactt
     8701 cttgaactct ccggcgctgc cactgcgttc gtagatcgtc ttgaacaacc atctggcttc
     8761 tgccttgcct gcggcgcggc gtgccaggcg gtagagaaaa cggccgatgc cgggatcgat
     8821 caaaaagtaa tcggggtgaa ccgtcagcac gtccgggttc ttgccttctg tgatctcgcg
     8881 gtacatccaa tcagctagct cgatctcgat gtactccggc cgcccggttt cgctctttac
     8941 gatcttgtag cggctaatca aggcttcacc ctcggatacc gtcaccaggc ggccgttctt
     9001 ggccttcttc gtacgctgca tggcaacgtg cgtggtgttt aaccgaatgc aggtttctac
     9061 caggtcgtct ttctgctttc cgccatcggc tcgccggcag aacttgagta cgtccgcaac
     9121 gtgtggacgg aacacgcggc cgggcttgtc tcccttccct tcccggtatc ggttcatgga
     9181 ttcggttaga tgggaaaccg ccatcagtac caggtcgtaa tcccacacac tggccatgcc
     9241 ggccggccct gcggaaacct ctacgtgccc gtctggaagc tcgtagcgga tcacctcgcc
     9301 agctcgtcgg tcacgcttcg acagacggaa aacggccacg tccatgatgc tgcgactatc
     9361 gcgggtgccc acgtcataga gcatcggaac gaaaaaatct ggttgctcgt cgcccttggg
     9421 cggcttccta atcgacgacg caccggctgc cggcggttgc cgggattctt tgcggattcg
     9481 atcagcggcc gcttgccacg attcaccggg gcgtgcttct gcctcgatgc gttgccgctg
     9541 ggcggcctgc gcggccttca acttctccac caggtcatca cccagcgccg cgccgatttg
     9601 taccgggccg gatggtttgc gaccgctcac gccgattcct cgggcttggg ggttccagtg
     9661 ccattgcagg gccggcagac aacccagccg cttacgcctg gccaaccgcc cgttcctcca
     9721 cacatggggc attccacggc gtcggtgcct ggttgttctt gattttccat gccgcctcct
     9781 ttagccgcta aaattcatct ac
//
