LOCUS       sequence-377996-       10104 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Ti plasmid pICH86988 harbouring chl-roGFP2-ORP1 for peroxide 
            detection in the plastids of plant cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10104)
  AUTHORS   Exposito-Rodriguez M, Reeder B, Brooke GN, Hough MA, Laissue PP, 
            Mullineaux PM
  TITLE     A novel glutathione peroxidase-based biosensor disentangles 
            differential subcellular accumulation of H2O2 and lipid 
            hydroperoxides
  JOURNAL   bioRxiv 2024.01.18.576236
REFERENCE   2  (bases 1 to 10104)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.01.18.576236"
COMMENT     Sequence Label: pICH-chl-roGFP2-ORP1
FEATURES             Location/Qualifiers
     source          1..10104
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    7..31
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(154..742)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(234..253)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(913..1707)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCAKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     promoter        complement(1708..1812)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      2095..2289
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(2355..3428)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(3857..4486)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    4750..4774
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        4952..5131
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        4984..5167
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             5188..5982
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5242..5261)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5852..5871
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      6008..6260
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        7276..7621
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     7564..7584
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     terminator      9341..10048
                     /label=OCS terminator
                     /note="octopine synthase terminator"
ORIGIN
        1 agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt attagaataa
       61 tcggatattt aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat gtgccaacca
      121 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
      181 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
      241 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
      301 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
      361 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
      421 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
      481 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
      541 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
      601 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
      661 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
      721 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
      781 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
      841 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
      901 ttggtctgac agctaaaaca attcatccag taaaatataa tattttattt tctcccaatc
      961 aggcttgatc cccagtaagt caaaaaatag ctcgacatac tgttcttccc cgatatcctc
     1021 cctgatcgac cggacgcaga aggcaatgtc ataccacttg tccgccctgc cgcttctccc
     1081 aagatcaata aagccactta ctttgccatc tttcacaaag atgttgctgt ctcccaggtc
     1141 gccgtgggaa aagacaagtt cctcttcggg cttttccgtc tttaaaaaat catacagctc
     1201 gcgcggatct ttaaatggag tgtcctcttc ccagttttcg caatccacat cggccagatc
     1261 gttattcagt aagtaatcca attcggctaa gcggctgtct aagctattcg tatagggaca
     1321 atccgatatg tcgatggagt gaaagagcct gatgcactcc gcatacagct cgataatctt
     1381 ttcagggctt tgttcatctt catactcttc cgagcaaagg acgccatcgg cctcactcat
     1441 gagcagattg ctccagccat catgccgttc aaagtgcagg acctttggaa caggcagctt
     1501 tccttccagc catagcatca tgtccttttc ccgttccaca tcataggtgg tccctttata
     1561 ccggctgtcc gtcattttta aatataggtt ttcattttct cccaccagct tatatacctt
     1621 agcaggagac attccttccg tatcttttgc gcagcggtat ttttcgatca gttttttcaa
     1681 ttccggtgat attctcattt tagccatact cttccttttt caatattatt gaagcattta
     1741 tcaaggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     1801 aggggttccg cgcacgaatt ggccagcgct gccatttttg gggtgaggcc gttcgcggcc
     1861 gaggggcgca gcccctgggg ggatgggagc tcggtgcctt gatgtgggcg ccggcggtcg
     1921 agtggcgacg gcgcggcttg tccgcgccct ggtagattgc ctggccgtag gccagccatt
     1981 tttgagcggc cagcggccgc gataggccga cgcgaagcgg cggggcgtag ggagcgcagc
     2041 gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc gctggccaga cagttatgca
     2101 caggccaggc gggttttaag agttttaata agttttaaag agttttaggc ggaaaaatcg
     2161 ccttttttct cttttatatc agtcacttac atgtgtgacc ggttcccaat gtacggcttt
     2221 gggttcccaa tgtacgggtt ccggttccca atgtacggct ttgggttccc aatgtacgtg
     2281 ctatccacag gaaagaagct ttttcgacct ttttcccctg ctagggcaat ttgccctagc
     2341 atctgctccg tacattagga accggcggat gcttcgccct cgatcaggtt gcggtagcgc
     2401 atgactagga tcgggccagc ctgccccgcc tcctccttca aatcgtactc cggcaggtca
     2461 tttgacccga tcagcttgcg cacggtgaaa cagaacttct tgaactctcc ggcgctgcca
     2521 ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg ccttgcctgc ggcgcggcgt
     2581 gccaggcggt agagaaaacg gccgatgccg ggatcgatca aaaagtaatc ggggtgaacc
     2641 gtcagcacgt ccgggttctt gccttctgtg atctcgcggt acatccaatc agctagctcg
     2701 atctcgatgt actccggccg cccggtttcg ctctttacga tcttgtagcg gctaatcaag
     2761 gcttcaccct cggataccgt caccaggcgg ccgttcttgg ccttcttcgt acgctgcatg
     2821 gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca ggtcgtcttt ctgctttccg
     2881 ccatcggctc gccggcagaa cttgagtacg tccgcaacgt gtggacggaa cacgcggccg
     2941 ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt cggttagatg ggaaaccgcc
     3001 atcagtacca ggtcgtaatc ccacacactg gccatgccgg ccggccctgc ggaaacctct
     3061 acgtgcccgt ctggaagctc gtagcggatc acctcgccag ctcgtcggtc acgcttcgac
     3121 agacggaaaa cggccacgtc catgatgctg cgactatcgc gggtgcccac gtcatagagc
     3181 atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg gcttcctaat cgacgacgca
     3241 ccggctgccg gcggttgccg ggattctttg cggattcgat cagcggccgc ttgccacgat
     3301 tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg cggcctgcgc ggccttcaac
     3361 ttctccacca ggtcatcacc cagcgccgcg ccgatttgta ccgggccgga tggtttgcga
     3421 ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc attgcagggc cggcagacaa
     3481 cccagccgct tacgcctggc caaccgcccg ttcctccaca catggggcat tccacggcgt
     3541 cggtgcctgg ttgttcttga ttttccatgc cgcctccttt agccgctaaa attcatctac
     3601 tcatttattc atttgctcat ttactctggt agctgcgcga tgtattcaga tagcagctcg
     3661 gtaatggtct tgccttggcg taccgcgtac atcttcagct tggtgtgatc ctccgccggc
     3721 aactgaaagt tgacccgctt catggctggc gtgtctgcca ggctggccaa cgttgcagcc
     3781 ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt ttgtgctttt gctcattttc
     3841 tctttacctc attaactcaa atgagttttg atttaatttc agcggccagc gcctggacct
     3901 cgcgggcagc gtcgccctcg ggttctgatt caagaacggt tgtgccggcg gcggcagtgc
     3961 ctgggtagct cacgcgctgc gtgatacggg actcaagaat gggcagctcg tacccggcca
     4021 gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat cgcccgcgac acgacaaagg
     4081 ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt aaccagctcc accaggtcgg
     4141 cggtggccca tatgtcgtaa gggcttggct gcaccggaat cagcacgaag tcggctgcct
     4201 tgatcgcgga cacagccaag tccgccgcct ggggcgctcc gtcgatcact acgaagtcgc
     4261 gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg gtcgatgccg acaacggtta
     4321 gcggttgatc ttcccgcacg gccgcccaat cgcgggcact gccctgggga tcggaatcga
     4381 ctaacagaac atcggccccg gcgagttgca gggcgcgggc tagatgggtt gcgatggtcg
     4441 tcttgcctga cccgcctttc tggttaagta cagcgataac cttcatgcgt tccccttgcg
     4501 tatttgttta tttactcatc gcatcatata cgcagcgacc gcatgacgca agctgtttta
     4561 ctcaaataca catcaccttt ttagacggcg gcgctcggtt tcttcagcgg ccaagctggc
     4621 cggccaggcc gccagcttgg catcagacaa accggccagg atttcatgca gccgcacggc
     4681 tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg
     4741 gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa
     4801 cacattgcgg acgtttttaa tgtactgggg tggatgcagt gggccccact ctgtgtgccg
     4861 aattcggatc cggagagcgg agaattaagg gagtcacgtt atgacccccg ccgatgacgc
     4921 gggacaagcc gttttacgtt tggaactgac agaaccgcaa cgttgaagga gccactcagc
     4981 cgcgggtttc tggagtttaa tgagctaagc acatacgtca gaaaccatta ttgcgcgttc
     5041 aaaagtcgcc taaggtcact atcagctagc aaatatttct tgtcaaaaat gctccactga
     5101 cgttccataa attcccctcg gtatccaatt agagtctcat attcactctc aatccaaata
     5161 atctgcaccg gatctggatc gtttcgcatg attgaacaag atggattgca cgcaggttct
     5221 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc
     5281 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc
     5341 gacctgtccg gtgccctgaa tgaactccag gacgaggcag cgcggctatc gtggctggcc
     5401 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg
     5461 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag
     5521 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc
     5581 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt
     5641 cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
     5701 gccaggctca aggcgcgtat gcccgacggc gaggatctcg tcgtgactca tggcgatgcc
     5761 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg
     5821 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag
     5881 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg
     5941 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggtct ggatctagag
     6001 tcaagcagat cgttcaaaca tttggcaata aagtttctta agattgaatc ctgttgccgg
     6061 tcttgcgatg attatcatat aatttctgtt gaattacgtt aagcatgtaa taattaacat
     6121 gtaatgcatg acgttattta tgagatgggt ttttatgatt agagtcccgc aattatacat
     6181 ttaatacgcg atagaaaaca aaatatagcg cgcaaactag gataaattat cgcgcgcggt
     6241 gtcatctatg ttactagatc gacgctgcaa gaattcaagc ttggaggaat tccaatccca
     6301 caaaaatctg agcttaacag cacagttgct cctctcagag cagaatcggg tattcaacac
     6361 cctcatatca actactacgt tgtgtataac ggtccacatg ccggtatata cgatgactgg
     6421 ggttgtacaa aggcggcaac aaacggcgtt cccggagttg cacacaagaa atttgccact
     6481 attacagagg caagagcagc agctgacgcg tacacaacaa gtcagcaaac agacaggttg
     6541 aacttcatcc ccaaaggaga agctcaactc aagcccaaga gctttgctaa ggccctaaca
     6601 agcccaccaa agcaaaaagc ccactggctc acgctaggaa ccaaaaggcc cagcagtgat
     6661 ccagccccaa aagagatctc ctttgccccg gagattacaa tggacgattt cctctatctt
     6721 tacgatctag gaaggaagtt cgaaggtgaa ggtgacgaca ctatgttcac cactgataat
     6781 gagaaggtta gcctcttcaa tttcagaaag aatgctgacc cacagatggt tagagaggcc
     6841 tacgcagcaa gtctcatcaa gacgatctac ccgagtaaca atctccagga gatcaaatac
     6901 cttcccaaga aggttaaaga tgcagtcaaa agattcagga ctaattgcat caagaacaca
     6961 gagaaagaca tatttctcaa gatcagaagt actattccag tatggacgat tcaaggcttg
     7021 cttcataaac caaggcaagt aatagagatt ggagtctcta aaaaggtagt tcctactgaa
     7081 tctaaggcca tgcatggagt ctaagattca aatcgaggat ctaacagaac tcgccgtcaa
     7141 gactggcgaa cagttcatac agagtctttt acgactcaat gacaagaaga aaatcttcgt
     7201 caacatggtg gagcacgaca ctctggtcta ctccaaaaat gtcaaagata cagtctcaga
     7261 agatcaaagg gctattgaga cttttcaaca aaggataatt tcgggaaacc tcctcggatt
     7321 ccattgccca gctatctgtc acttcatcga aaggacagta gaaaaggaag gtggctccta
     7381 caaatgccat cattgcgata aaggaaaggc tatcattcaa gatctctctg ccgacagtgg
     7441 tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagagg ttccaaccac
     7501 gtctacaaag caagtggatt gatgtgacat ctccactgac gtaagggatg acgcacaatc
     7561 ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggac
     7621 acgctcgagt ataagagctc atttttacaa caattaccaa caacaacaaa caacaaacaa
     7681 cattacaatt acatttacaa ttatcgatac aatggtataa gagctcattt ttacaacaat
     7741 taccaacaac aacaaacaac aaacaacatt acaattacat ttacaattat cgataacatg
     7801 gcttcttcta tgctttcttc tgctgctgtt gttgctactc gtgctagtgc tgctcaagct
     7861 agtatggttg ctccttttac tggacttaag tctgctgctt cttttcctgt tactagaaag
     7921 caaaacaacc ttgatattac ttctattgct agtaacggag gaagagttcg agcaaagctt
     7981 aacttctttc agcagttctt cacctttgct aaccataagc ttatggttag caaaggtgaa
     8041 gaactgttca ccggtgttgt gccgatcctg gttgaactgg acggtgacgt taacggtcac
     8101 aaattctctg tttccggtga aggtgaaggt gacgctacct acggtaaact gaccctgaaa
     8161 ttcatctcta ccaccggtaa actgccggtt ccgtggccga ccctggtgac caccctgacc
     8221 tacggtgttc agtgcttctc ccgttacccg gaccacatga aacagcacga tttcttcaaa
     8281 tccgcgatgc cggaaggtta cgtgcaggaa cgtaccatct tcttcaaaga tgacggtaac
     8341 tacaaaaccc gtgctgaagt taaattcgaa ggtgacaccc tggttaaccg tattgaactg
     8401 aaaggcatcg acttcaaaga agatggtaac atcctgggtc acaaactgga atacaactac
     8461 aactgtcaca acgtttacat catggcggat aaacagaaaa acggtatcaa agttaacttc
     8521 aaaatccgtc acaacatcga agatggttct gttcagctgg ctgatcacta ccagcagaac
     8581 accccgatcg gtgacggtcc ggttctgctg ccggacaacc actacctgtc tacctgctcc
     8641 gcgctgagca aagacccgaa cgaaaaacgt gaccacatgg ttctgctgga attcgttacc
     8701 gcggcgggta tcaccctggg catggatgaa ctgtacaaaa cctccggcgg ttctggtggc
     8761 ggtggctctg gtggtggtgg ttctggtggt ggcggctctg gtggtggtgg ttctggtggc
     8821 ggtggttctg gcggcgaatt cgacatcagc gaattctaca aactggctcc ggttgacaaa
     8881 aaaggtcagc cgttcccgtt cgaccagctg aaaggtaaag tggtgctgat cgttaacgtt
     8941 gcatctaaat gcggtttcac cccgcagtac aaagaactgg aagcgctgta taaacgttac
     9001 aaagatgaag gtttcactat catcggcttc ccgtgtaacc agttcggtca ccaggaaccg
     9061 ggctctgatg aagaaatcgc ccagttctgc caactgaact atggcgtgac tttcccgatc
     9121 atgaaaaaga tcgacgttaa cggtggtaac gaagatccgg tttataaatt cctgaagagc
     9181 caaaaatccg gtatgttggg cttgagaggt atcaaatgga acttcgaaaa attcctggtt
     9241 gacaaaaaag gtaaagttta cgaacgttat agctctctga ccaaaccgtc ctctctgtct
     9301 gaaaccatcg aagaactgct gaaagaagtt taagcttgtc ctgctttaat gagatatgcg
     9361 agaagcctat gatcgcatga tatttgcttt caattctgtt gtgcacgttg taaaaaacct
     9421 gagcatgtgt agctcagatc cttaccgccg gtttcggttc attctaatga atatatcacc
     9481 cgttactatc gtatttttat gaataatatt ctccgttcaa tttactgatt gtaccctact
     9541 acttatatgt acaatattaa aatgaaaaca atatattgtg ctgaataggt ttatagcgac
     9601 atctatgata gagcgccaca ataacaaaca attgcgtttt attattacaa atccaatttt
     9661 aaaaaaagcg gcagaaccgg tcaaacctaa aagactgatt acataaatct tattcaaatt
     9721 tcaaaagtgc cccaggggct agtatctacg acacaccgag cggcgaacta ataacgctca
     9781 ctgaagggaa ctccggttcc ccgccggcgc gcatgggtga gattccttga agttgagtat
     9841 tggccgtccg ctctaccgaa agttacgggc accattcaac ccggtccagc acggcggccg
     9901 ggtaaccgac ttgctgcccc gagaattatg cagcattttt ttggtgtatg tgggccccaa
     9961 atgaagtgca ggtcaaacct tgacagtgac gacaaatcgt tgggcgggtc cagggcgaat
    10021 tttgcgacaa catgtcgagg ctcagcagga ccgctactag aggatgcaca tgtgaccgag
    10081 ggacacgaag tggtttaaac tatc
//
