LOCUS       sequence-377997-        9487 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  On state for the second generation of NOT gates, which target the 
            Rluc promoter to repress expression via its FRT sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9487)
  AUTHORS   Lloyd JPB, Ly F, Gong P, Pflueger J, Swain T, Pflueger C, Fourie E, 
            Khan MA, Kidd BN, Lister R
  TITLE     Synthetic memory circuits for stable cell reprogramming in plants.
  JOURNAL   Nat Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2.
  PUBMED    35788565
REFERENCE   2  (bases 1 to 9487)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2."
COMMENT     Sequence Label: LLP714_L2pV3_NOT_FRT_Rluc_0I_lacZ
FEATURES             Location/Qualifiers
     source          1..9487
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    22..55
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    843..876
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     misc_feature    892..947
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     intron          979..1167
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     polyA_signal    2195..2369
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             2757..4409
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(2823..2841)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      4429..4629
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     protein_bind    4700..4721
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4736..4741,4742..4759,4760..4766)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4774..4790
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4779..4801
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4798..4814
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4798..4814
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4810..5133
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    4827..4883
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(4884..4901)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4884..4900)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4893..4915)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5109..5128)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    5265..5289
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      5377..6087
                     /label=oriV
                     /note="incP origin of replication"
     promoter        6108..6212
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6213..7007
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      7178..7766
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7667..7686
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             8102..9250
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    9396..9420
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgaatt cggatccgga ggaagttcct attctctaga aagtatagga acttctcgac
       61 aaaatttaga acgaacttaa ttatgatctc aaatacattg atacatatct catctagatc
      121 taggttatca ttatgtaaga aagttttgac gaatatggca cgacaaaatg gctagactcg
      181 atgtaattgg tatctcaact caacattata cttataccaa acattagtta gacaaaattt
      241 aaacaactat tttttatgta tgcaagagtc agcatatgta taattgattc agaatcgttt
      301 tgacgagttc ggatgtagta gtagccatta tttaatgtac atactaatcg tgaatagtga
      361 atatgatgaa acattgtatc ttattgtata aatatccata aacacatcat gaaagacact
      421 ttctttcacg gtctgaatta attatgatac aattctaata gaaaacgaat taaattacgt
      481 tgaattgtat gaaatctaat tgaacaagcc aaccacgacg acgactaacg ttgcctggat
      541 tgactcggtt taagttaacc actaaaaaaa cggagctgtc atgtaacacg cggatcgagc
      601 aggtcacagt catgaagcca tcaaagcaaa agaactaatc caagggctga gatgattaat
      661 tagtttaaaa attagttaac acgagggaaa aggctgtctg acagccaggt cacgttatct
      721 ttacctgtgg tcgaaatgat tcgtgtctgt cgattttaat tatttttttg aaaggccgaa
      781 aataaagttg taagagataa acccgcctat ataaattcat atattttcct ctccgctttg
      841 aagaagttcc tattctctag aaagtatagg aacttctact gtatttttac aacaattacc
      901 aacaacaaca aacaacaaac aacattacaa ttactattta caattacaat gacttcaaag
      961 gtgtatgatc ctgagcaggt aagtttctgc ttctaccttt gatatatata taataattat
     1021 cattaattag tagtaatata atatttcaaa tatttttttc aaaataaaag aatgtagtat
     1081 atagcaattg cttttctgta gtttataagt gtgtatattt taatttataa cttttctaat
     1141 atatgaccaa aatttgttga tgtgcagaga aaaagaatga taactggtcc acaatggtgg
     1201 gcaagatgta aacagatgaa cgtgcttgat tctttcatta attactacga ttcagaaaag
     1261 cacgctgaga atgcagttat ctttcttcac ggaaacgctg catcttcata tttgtggaga
     1321 catgttgtgc ctcacattga accagtggct aggtgtatta tcccagatct tatcggaatg
     1381 ggaaagtctg gtaaatctgg aaatggttct tacagacttt tggatcatta taagtacctc
     1441 acagcttggt tcgaacttct caacttacct aagaagatta ttttcgttgg acacgattgg
     1501 ggtgcttgct tggcattcca ttactcatac gaacaccaag ataagatcaa agctatagtt
     1561 catgcagaga gtgttgtgga tgtgatagaa tcttgggatg agtggccaga tattgaagag
     1621 gatattgctt tgatcaagtc tgaagaggga gaaaagatgg ttctcgaaaa taactttttc
     1681 gtggaaacta tgcttccttc aaagattatg agaaaattgg aaccagaaga gtttgctgca
     1741 tatctcgaac ctttcaagga aaaaggagag gttagaaggc caacactcag ttggcctagg
     1801 gaaataccat tagttaaggg aggtaaacct gatgttgtgc agatcgtgag aaattataac
     1861 gcttacctta gggcatcaga tgatttgcct aagatgttca tcgagtcaga tccaggattt
     1921 ttcagtaatg ctatagttga aggtgcaaag aaatttccaa acaccgagtt cgttaaggtg
     1981 aaaggattgc atttctcaca agaagatgct cctgatgaga tgggaaagta tattaagtct
     2041 tttgttgaaa gagtgctcaa aaacgagcag ggttctcgat ctgatgcttc tgaagtaacg
     2101 tcaacaagct cccagtctga ggtatgcaca gtggaaacgc caggttgtgt tggttcttaa
     2161 gcttctctag ctagagtcga tcgacaagct cgagtttctc cataataatg tgtgagtagt
     2221 tcccagataa gggaattagg gttcctatag ggtttcgctc atgtgttgag catataagaa
     2281 acccttagta tgtatttgta tttgtaaaat acttctatca ataaaatttc taattcctaa
     2341 aaccaaaatc cagtactaaa atccagatcg ctgcaagaat tcaagcttgg agccaacact
     2401 cgaatcccca cccgttggac caaacccggc tcattaagcg tcggttcaga tttatttcct
     2461 ttatttaaaa aaaaggaaag ggtaaaaaat agaaaattgg aaacagttaa agcccaaaat
     2521 tgtaatttac cgagaattgt aaatttacct gaaaacccta cgctatagtt tcgactataa
     2581 ataccaaact taggacctca cttcagaatc ccctcgtcgc tgcgtctctc tcccgcaacc
     2641 ttcgattttc gtttattcgc atccatcgga gagagaaaac aatcaataag cgacctacta
     2701 caaacaagta agtaagagaa aaaccaaaag aagaagagaa acaacaagaa gaagtaatgg
     2761 aggacgccaa aaacataaag aaaggcccgg cgccattcta tcctctagag gatggaaccg
     2821 ctggagagca actgcataag gctatgaaga gatacgccct ggttcctgga acaattgctt
     2881 ttacagatgc acatatcgag gtgaacatca cgtacgcgga atacttcgaa atgtccgttc
     2941 ggttggcaga agctatgaaa cgatatgggc tgaatacaaa tcacagaatc gtcgtatgca
     3001 gtgaaaactc tcttcaattc tttatgccgg tgttgggcgc gttatttatc ggagttgcag
     3061 ttgcgcccgc gaacgacatt tataatgaac gtgaattgct caacagtatg aacatttcgc
     3121 agcctaccgt agtgtttgtt tccaaaaagg ggttgcaaaa aattttgaac gtgcaaaaaa
     3181 aattaccaat aatccagaaa attattatca tggattctaa aacggattac cagggatttc
     3241 agtcgatgta cacgttcgtc acatctcatc tacctcccgg ttttaatgaa tacgattttg
     3301 taccagagtc ctttgatcgt gacaaaacaa ttgcactgat aatgaattcc tctggatcta
     3361 ctgggttacc taagggtgtg gcccttccgc atagaactgc ctgcgtcaga ttctcgcatg
     3421 ccagagatcc tatttttggc aatcaaatca ttccggatac tgcgatttta agtgttgttc
     3481 cattccatca cggttttgga atgtttacta cactcggata tttgatatgt ggatttcgag
     3541 tcgtcttaat gtatagattt gaagaagagc tgtttttacg atcccttcag gattacaaaa
     3601 ttcaaagtgc gttgctagta ccaaccctat tttcattctt cgccaaaagc actctgattg
     3661 acaaatacga tttatctaat ttacacgaaa ttgcttctgg gggcgcacct ctttcgaaag
     3721 aagtcgggga agcggttgca aaacgcttcc atcttccagg gatacgacaa ggatatgggc
     3781 tcactgagac tacatcagct attctgatta cacccgaggg ggatgataaa ccgggcgcgg
     3841 tcggtaaagt tgttccattt tttgaagcga aggttgtgga tctggatacc gggaaaacgc
     3901 tgggcgttaa tcagagaggc gaattatgtg tcagaggacc tatgattatg tccggttatg
     3961 taaacaatcc ggaagcgacc aacgccttga ttgacaagga tggatggcta cattctggag
     4021 acatagctta ctgggacgag gacgaacact tcttcatagt tgaccgcttg aagtctttaa
     4081 ttaaatacaa aggatatcag gtggcccccg ctgaattgga atcgatattg ttacaacacc
     4141 ccaacatctt cgacgcgggc gtggcaggtc tgcccgacga tgacgccggt gaacttcccg
     4201 ccgccgttgt tgttttggag cacggaaaga cgatgacgga aaaagagatc gtggattacg
     4261 tcgccagtca agtaacaacc gcgaaaaagt tgcgcggagg agttgtgttt gtggacgaag
     4321 taccgaaagg tcttaccgga aaactcgacg caagaaaaat cagagagatc ctcataaagg
     4381 ccaagaaggg cggaaagtcc aaattgtaag cttgatcccc cgtcgacagc tagctatatc
     4441 atcaatttat gtattacaca taatatcgca ctcagtcttt catctacggc aatgtaccag
     4501 ctgatataat cagttattga aatatttctg aatttaaact tgcatcaata aatttatgtt
     4561 tttgcttgga ctataatacc tgacttgtta ttttatcaat aaatatttaa actatatttc
     4621 tttcaagatc gctactatga gaccgcagct ggcacgacag gtttcccgac tggaaagcgg
     4681 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     4741 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     4801 gaaacagcta tgaccatgat tacgccaagc ttgcatgcct gcaggtcgac tctagaggat
     4861 ccccgggtac cgagctcgaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     4921 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     4981 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     5041 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc
     5101 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
     5161 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     5221 acggtctcag ggacacgaag tgatccgttt aaactatcag tgtttgacag gatatattgg
     5281 cgggtaaacc taagagaaaa gagcgtttat tagaataatc ggatatttaa aagggcgtga
     5341 aaaggtttat ccgttcgtcc atttgtatgt gccagccgcc tttgcgacgc tcaccgggct
     5401 ggttgccctc gccgctgggc tggcggccgt ctatggccct gcaaacgcgc cagaaacgcc
     5461 gtcgaagccg tgtgcgagac accgcggccg ccggcgttgt ggatacctcg cggaaaactt
     5521 ggccctcact gacagatgag gggcggacgt tgacacttga ggggccgact cacccggcgc
     5581 ggcgttgaca gatgaggggc aggctcgatt tcggccggcg acgtggagct ggccagcctc
     5641 gcaaatcggc gaaaacgcct gattttacgc gagtttccca cagatgatgt ggacaagcct
     5701 ggggataagt gccctgcggt attgacactt gaggggcgcg actactgaca gatgaggggc
     5761 gcgatccttg acacttgagg ggcagagtgc tgacagatga ggggcgcacc tattgacatt
     5821 tgaggggctg tccacaggca gaaaatccag catttgcaag ggtttccgcc cgtttttcgg
     5881 ccaccgctaa cctgtctttt aacctgcttt taaaccaata tttataaacc ttgtttttaa
     5941 ccagggctgc gccctgtgcg cgtgaccgcg cacgccgaag gggggtgccc ccccttctcg
     6001 aaccctcccg gcccgctaac gcgggcctcc catcccccca ggggctgcgc ccctcggccg
     6061 cgaacggcct caccccaaaa atggcagcgc tggccaattc ccgagtgcgc ggaaccccta
     6121 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     6181 aaatgcttca ataatattga aaaaggaaga gtatggctaa aatgagaata tcaccggaat
     6241 tgaaaaaact gatcgaaaaa taccgctgcg taaaagatac ggaaggaatg tctcctgcta
     6301 aggtatataa gctggtggga gaaaatgaaa acctatattt aaaaatgacg gacagccggt
     6361 ataaagggac cacctatgat gtggaacggg aaaaggacat gatgctatgg ctggaaggaa
     6421 agctgcctgt tccaaaggtc ctgcactttg aacggcatga tggctggagc aatctgctca
     6481 tgagtgaggc cgatggcgtc ctttgctcgg aagagtatga agatgaacaa agccctgaaa
     6541 agattatcga gctgtatgcg gagtgcatca ggctctttca ctccatcgac atatcggatt
     6601 gtccctatac gaatagctta gacagccgct tagccgaatt ggattactta ctgaataacg
     6661 atctggccga tgtggattgc gaaaactggg aagaggacac tccatttaaa gatccgcgcg
     6721 agctgtatga ttttttaaag acggaaaagc ccgaagagga acttgtcttt tcccacggcg
     6781 acctgggaga cagcaacatc tttgtgaaag atggcaaagt aagtggcttt attgatcttg
     6841 ggagaagcgg cagggcggac aagtggtatg acattgcctt ctgcgtccgg tcgctcaggg
     6901 aggatatcgg ggaagaacag tatgtcgagc tattttttga cttactgggg atcaagcctg
     6961 attgggagaa aataaaatat tatattttac tggatgaatt gttttagctg tcagaccaag
     7021 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     7081 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     7141 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     7201 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     7261 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     7321 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     7381 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     7441 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     7501 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     7561 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     7621 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     7681 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     7741 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     7801 cagatcctag atgtggcgca acgatgccgg cgacaagcag gagcgcaccg acttcttccg
     7861 catcaagtgt tttggctctc aggccgaggc ccacggcaag tatttgggca aggggtcgct
     7921 ggtattcgtg cagggcaaga ttcggaatac caagtacgag aaggacggcc agacggtcta
     7981 cgggaccgac ttcattgccg ataaggtgga ttatctggac accaaggcac caggcgggtc
     8041 aaatcaggaa taagggcaca ttgccccggc gtgagtcggg gcaatcccgc aaggagggtg
     8101 aatgaatcgg acgtttgacc ggaaggcata caggcaagaa ctgatcgacg cggggttttc
     8161 cgccgaggat gccgaaacca tcgcaagccg caccgtcatg cgtgcgcccc gcgaaacctt
     8221 ccagtccgtc ggctcgatgg tccagcaagc tacggccaag atcgagcgcg acagcgtgca
     8281 actggctccc cctgccctgc ccgcgccatc ggccgccgtg gagcgttcgc gtcgtcttga
     8341 acaggaggcg gcaggtttgg cgaagtcgat gaccatcgac acgcgaggaa ctatgacgac
     8401 caagaagcga aaaaccgccg gcgaggacct ggcaaaacag gtcagcgagg ccaagcaggc
     8461 cgcgttgctg aaacacacga agcagcagat caaggaaatg cagctttcct tgttcgatat
     8521 tgcgccgtgg ccggacacga tgcgagcgat gccaaacgac acggcccgct ctgccctgtt
     8581 caccacgcgc aacaagaaaa tcccgcgcga ggcgctgcaa aacaaggtca ttttccacgt
     8641 caacaaggac gtgaagatca cctacaccgg cgtcgagctg cgggccgacg atgacgaact
     8701 ggtgtggcag caggtgttgg agtacgcgaa gcgcacccct atcggcgagc cgatcacctt
     8761 cacgttctac gagctttgcc aggacctggg ctggtcgatc aatggccggt attacacgaa
     8821 ggccgaggaa tgcctgtcgc gcctacaggc gacggcgatg ggcttcacgt ccgaccgcgt
     8881 tgggcacctg gaatcggtgt cgctgctgca ccgcttccgc gtcctggacc gtggcaagaa
     8941 aacgtcccgt tgccaggtcc tgatcgacga ggaaatcgtc gtgctgtttg ctggcgacca
     9001 ctacacgaaa ttcatatggg agaagtaccg caagctgtcg ccgacggccc gacggatgtt
     9061 cgactatttc agctcgcacc gggagccgta cccgctcaag ctggaaacct tccgcctcat
     9121 gtgcggatcg gattccaccc gcgtgaagaa gtggcgcgag caggtcggcg aagcctgcga
     9181 agagttgcga ggcagcggcc tggtggaaca cgcctgggtc aatgatgacc tggtgcattg
     9241 caaacgctag ggccttgtgg ggtcagttcc ggctgggggt tcagcagccc ctgctcggat
     9301 ctgttggacc ggacagtagt catggttgat gggctgcctg tatcgagtgg tgattttgtg
     9361 ccgagctgcc ggtcggggag ctgttggctg gctggtggca ggatatattg tggtgtaaac
     9421 aaattgacgc ttagacaact taataacaca ttgcggacgt ttttaatgta ctggggttga
     9481 acactct
//
