LOCUS       sequence-377999-       10134 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Ti plasmid pICH86988 harbouring chl-roGFP2-synORP1 for H2O2 
            detection in the plastids of plant cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10134)
  AUTHORS   Exposito-Rodriguez M, Reeder B, Brooke GN, Hough MA, Laissue PP, 
            Mullineaux PM
  TITLE     A novel glutathione peroxidase-based biosensor disentangles 
            differential subcellular accumulation of H2O2 and lipid 
            hydroperoxides
  JOURNAL   bioRxiv 2024.01.18.576236
REFERENCE   2  (bases 1 to 10134)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.01.18.576236"
COMMENT     Sequence Label: pICH-chl-roGFP2-synORP1
FEATURES             Location/Qualifiers
     source          1..10134
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(604..1233)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    1497..1521
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        1699..1878
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        1731..1914
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             1935..2729
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1989..2008)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2599..2618
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      2755..3007
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        4023..4368
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     4311..4331
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     terminator      6118..6825
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     misc_feature    6888..6912
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(7035..7623)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7115..7134)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7794..8588)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCAKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     promoter        complement(8589..8693)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8976..9170
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(join(9236..10134,1..175))
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
ORIGIN
        1 gttgccggga ttctttgcgg attcgatcag cggccgcttg ccacgattca ccggggcgtg
       61 cttctgcctc gatgcgttgc cgctgggcgg cctgcgcggc cttcaacttc tccaccaggt
      121 catcacccag cgccgcgccg atttgtaccg ggccggatgg tttgcgaccg ctcacgccga
      181 ttcctcgggc ttgggggttc cagtgccatt gcagggccgg cagacaaccc agccgcttac
      241 gcctggccaa ccgcccgttc ctccacacat ggggcattcc acggcgtcgg tgcctggttg
      301 ttcttgattt tccatgccgc ctcctttagc cgctaaaatt catctactca tttattcatt
      361 tgctcattta ctctggtagc tgcgcgatgt attcagatag cagctcggta atggtcttgc
      421 cttggcgtac cgcgtacatc ttcagcttgg tgtgatcctc cgccggcaac tgaaagttga
      481 cccgcttcat ggctggcgtg tctgccaggc tggccaacgt tgcagccttg ctgctgcgtg
      541 cgctcggacg gccggcactt agcgtgtttg tgcttttgct cattttctct ttacctcatt
      601 aactcaaatg agttttgatt taatttcagc ggccagcgcc tggacctcgc gggcagcgtc
      661 gccctcgggt tctgattcaa gaacggttgt gccggcggcg gcagtgcctg ggtagctcac
      721 gcgctgcgtg atacgggact caagaatggg cagctcgtac ccggccagcg cctcggcaac
      781 ctcaccgccg atgcgcgtgc ctttgatcgc ccgcgacacg acaaaggccg cttgtagcct
      841 tccatccgtg acctcaatgc gctgcttaac cagctccacc aggtcggcgg tggcccatat
      901 gtcgtaaggg cttggctgca ccggaatcag cacgaagtcg gctgccttga tcgcggacac
      961 agccaagtcc gccgcctggg gcgctccgtc gatcactacg aagtcgcgcc ggccgatggc
     1021 cttcacgtcg cggtcaatcg tcgggcggtc gatgccgaca acggttagcg gttgatcttc
     1081 ccgcacggcc gcccaatcgc gggcactgcc ctggggatcg gaatcgacta acagaacatc
     1141 ggccccggcg agttgcaggg cgcgggctag atgggttgcg atggtcgtct tgcctgaccc
     1201 gcctttctgg ttaagtacag cgataacctt catgcgttcc ccttgcgtat ttgtttattt
     1261 actcatcgca tcatatacgc agcgaccgca tgacgcaagc tgttttactc aaatacacat
     1321 caccttttta gacggcggcg ctcggtttct tcagcggcca agctggccgg ccaggccgcc
     1381 agcttggcat cagacaaacc ggccaggatt tcatgcagcc gcacggctga tgggctgcct
     1441 gtatcgagtg gtgattttgt gccgagctgc cggtcgggga gctgttggct ggctggtggc
     1501 aggatatatt gtggtgtaaa caaattgacg cttagacaac ttaataacac attgcggacg
     1561 tttttaatgt actggggtgg atgcagtggg ccccactctg tgtgccgaat tcggatccgg
     1621 agagcggaga attaagggag tcacgttatg acccccgccg atgacgcggg acaagccgtt
     1681 ttacgtttgg aactgacaga accgcaacgt tgaaggagcc actcagccgc gggtttctgg
     1741 agtttaatga gctaagcaca tacgtcagaa accattattg cgcgttcaaa agtcgcctaa
     1801 ggtcactatc agctagcaaa tatttcttgt caaaaatgct ccactgacgt tccataaatt
     1861 cccctcggta tccaattaga gtctcatatt cactctcaat ccaaataatc tgcaccggat
     1921 ctggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     1981 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     2041 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     2101 ccctgaatga actccaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     2161 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     2221 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
     2281 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     2341 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     2401 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     2461 cgcgtatgcc cgacggcgag gatctcgtcg tgactcatgg cgatgcctgc ttgccgaata
     2521 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     2581 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     2641 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     2701 tctatcgcct tcttgacgag ttcttctgag cgggtctgga tctagagtca agcagatcgt
     2761 tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt
     2821 atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg
     2881 ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata
     2941 gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta
     3001 ctagatcgac gctgcaagaa ttcaagcttg gaggaattcc aatcccacaa aaatctgagc
     3061 ttaacagcac agttgctcct ctcagagcag aatcgggtat tcaacaccct catatcaact
     3121 actacgttgt gtataacggt ccacatgccg gtatatacga tgactggggt tgtacaaagg
     3181 cggcaacaaa cggcgttccc ggagttgcac acaagaaatt tgccactatt acagaggcaa
     3241 gagcagcagc tgacgcgtac acaacaagtc agcaaacaga caggttgaac ttcatcccca
     3301 aaggagaagc tcaactcaag cccaagagct ttgctaaggc cctaacaagc ccaccaaagc
     3361 aaaaagccca ctggctcacg ctaggaacca aaaggcccag cagtgatcca gccccaaaag
     3421 agatctcctt tgccccggag attacaatgg acgatttcct ctatctttac gatctaggaa
     3481 ggaagttcga aggtgaaggt gacgacacta tgttcaccac tgataatgag aaggttagcc
     3541 tcttcaattt cagaaagaat gctgacccac agatggttag agaggcctac gcagcaagtc
     3601 tcatcaagac gatctacccg agtaacaatc tccaggagat caaatacctt cccaagaagg
     3661 ttaaagatgc agtcaaaaga ttcaggacta attgcatcaa gaacacagag aaagacatat
     3721 ttctcaagat cagaagtact attccagtat ggacgattca aggcttgctt cataaaccaa
     3781 ggcaagtaat agagattgga gtctctaaaa aggtagttcc tactgaatct aaggccatgc
     3841 atggagtcta agattcaaat cgaggatcta acagaactcg ccgtcaagac tggcgaacag
     3901 ttcatacaga gtcttttacg actcaatgac aagaagaaaa tcttcgtcaa catggtggag
     3961 cacgacactc tggtctactc caaaaatgtc aaagatacag tctcagaaga tcaaagggct
     4021 attgagactt ttcaacaaag gataatttcg ggaaacctcc tcggattcca ttgcccagct
     4081 atctgtcact tcatcgaaag gacagtagaa aaggaaggtg gctcctacaa atgccatcat
     4141 tgcgataaag gaaaggctat cattcaagat ctctctgccg acagtggtcc caaagatgga
     4201 cccccaccca cgaggagcat cgtggaaaaa gaagaggttc caaccacgtc tacaaagcaa
     4261 gtggattgat gtgacatctc cactgacgta agggatgacg cacaatccca ctatccttcg
     4321 caagaccctt cctctatata aggaagttca tttcatttgg agaggacacg ctcgagtata
     4381 agagctcatt tttacaacaa ttaccaacaa caacaaacaa caaacaacat tacaattaca
     4441 tttacaatta tcgatacaat ggtataagag ctcattttta caacaattac caacaacaac
     4501 aaacaacaaa caacattaca attacattta caattatcga taacatggct tcttctatgc
     4561 tttcttctgc tgctgttgtt gctactcgtg ctagtgctgc tcaagctagt atggttgctc
     4621 cttttactgg acttaagtct gctgcttctt ttcctgttac tagaaagcaa aacaaccttg
     4681 atattacttc tattgctagt aacggaggaa gagttcgagc aaagcttatg gttagcaaag
     4741 gtgaagaact gttcaccggt gttgtgccga tcctggttga actggacggt gacgttaacg
     4801 gtcacaaatt ctctgtttcc ggtgaaggtg aaggtgacgc tacctacggt aaactgaccc
     4861 tgaaattcat ctctaccacc ggtaaactgc cggttccgtg gccgaccctg gtgaccaccc
     4921 tgacctacgg tgttcagtgc ttctcccgtt acccggacca catgaaacag cacgatttct
     4981 tcaaatccgc gatgccggaa ggttacgtgc aggaacgtac catcttcttc aaagatgacg
     5041 gtaactacaa aacccgtgct gaagttaaat tcgaaggtga caccctggtt aaccgtattg
     5101 aactgaaagg catcgacttc aaagaagatg gtaacatcct gggtcacaaa ctggaataca
     5161 actacaactg tcacaacgtt tacatcatgg cggataaaca gaaaaacggt atcaaagtta
     5221 acttcaaaat ccgtcacaac atcgaagatg gttctgttca gctggctgat cactaccagc
     5281 agaacacccc gatcggtgac ggtccggttc tgctgccgga caaccactac ctgtctacct
     5341 gctccgcgct gagcaaagac ccgaacgaaa aacgtgacca catggttctg ctggaattcg
     5401 ttaccgcggc gggtatcacc ctgggcatgg atgaactgta caaaacctcc ggcggttctg
     5461 gtggcggtgg ctctggtggt ggtggttctg gtggtggcgg ctctggtggt ggtggttctg
     5521 gtggcggtgg ttctggcggc gaattcgaca tcagcgaatt ctacaaactg gctccggttg
     5581 acaaaaaagg tcagccgttc ccgttcgacc agctgaaagg taaagtggtg ctgatcgtta
     5641 acgttgcatc taaatgcggt ttcaccccgc agtacaaaga actggaagcg ctgtataaac
     5701 gttacaaaga tgaaggtttc actatcatcg gcttcccgtg taaccagttc ggtcaccagg
     5761 aaccgggctc tgatgaagaa atcctgaact ctctgaaata cgttcgtccg ggtggtggtt
     5821 tcgaaccgaa cttcccgatc atgaaaaaga tcgacgttaa cggtggtaac gaagatccgg
     5881 tttataaatt cctgcgtgaa gcgctgccgg ctccgtctga tgacgcgacc gcgctgatga
     5941 ccgatccgaa actgatcacc tggagcccgg tttgccgtaa cgatgttgcg tggaacttcg
     6001 aaaaattcct ggttgacaaa aaaggtaaag tttacgaacg ttatagctct ctgaccaaac
     6061 cgtcctctct gtctgaaacc atcgaagaac tgctgaaaga agttgaataa gcttgtcctg
     6121 ctttaatgag atatgcgaga agcctatgat cgcatgatat ttgctttcaa ttctgttgtg
     6181 cacgttgtaa aaaacctgag catgtgtagc tcagatcctt accgccggtt tcggttcatt
     6241 ctaatgaata tatcacccgt tactatcgta tttttatgaa taatattctc cgttcaattt
     6301 actgattgta ccctactact tatatgtaca atattaaaat gaaaacaata tattgtgctg
     6361 aataggttta tagcgacatc tatgatagag cgccacaata acaaacaatt gcgttttatt
     6421 attacaaatc caattttaaa aaaagcggca gaaccggtca aacctaaaag actgattaca
     6481 taaatcttat tcaaatttca aaagtgcccc aggggctagt atctacgaca caccgagcgg
     6541 cgaactaata acgctcactg aagggaactc cggttccccg ccggcgcgca tgggtgagat
     6601 tccttgaagt tgagtattgg ccgtccgctc taccgaaagt tacgggcacc attcaacccg
     6661 gtccagcacg gcggccgggt aaccgacttg ctgccccgag aattatgcag catttttttg
     6721 gtgtatgtgg gccccaaatg aagtgcaggt caaaccttga cagtgacgac aaatcgttgg
     6781 gcgggtccag ggcgaatttt gcgacaacat gtcgaggctc agcaggaccg ctactagagg
     6841 atgcacatgt gaccgaggga cacgaagtgg tttaaactat cagtgtttga caggatatat
     6901 tggcgggtaa acctaagaga aaagagcgtt tattagaata atcggatatt taaaagggcg
     6961 tgaaaaggtt tatccgttcg tccatttgta tgtgccaacc acaggaaccg taaaaaggcc
     7021 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     7081 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     7141 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     7201 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     7261 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     7321 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     7381 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     7441 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     7501 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     7561 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     7621 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     7681 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     7741 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagctaaaac
     7801 aattcatcca gtaaaatata atattttatt ttctcccaat caggcttgat ccccagtaag
     7861 tcaaaaaata gctcgacata ctgttcttcc ccgatatcct ccctgatcga ccggacgcag
     7921 aaggcaatgt cataccactt gtccgccctg ccgcttctcc caagatcaat aaagccactt
     7981 actttgccat ctttcacaaa gatgttgctg tctcccaggt cgccgtggga aaagacaagt
     8041 tcctcttcgg gcttttccgt ctttaaaaaa tcatacagct cgcgcggatc tttaaatgga
     8101 gtgtcctctt cccagttttc gcaatccaca tcggccagat cgttattcag taagtaatcc
     8161 aattcggcta agcggctgtc taagctattc gtatagggac aatccgatat gtcgatggag
     8221 tgaaagagcc tgatgcactc cgcatacagc tcgataatct tttcagggct ttgttcatct
     8281 tcatactctt ccgagcaaag gacgccatcg gcctcactca tgagcagatt gctccagcca
     8341 tcatgccgtt caaagtgcag gacctttgga acaggcagct ttccttccag ccatagcatc
     8401 atgtcctttt cccgttccac atcataggtg gtccctttat accggctgtc cgtcattttt
     8461 aaatataggt tttcattttc tcccaccagc ttatatacct tagcaggaga cattccttcc
     8521 gtatcttttg cgcagcggta tttttcgatc agttttttca attccggtga tattctcatt
     8581 ttagccatac tcttcctttt tcaatattat tgaagcattt atcaaggtta ttgtctcatg
     8641 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacgaat
     8701 tggccagcgc tgccattttt ggggtgaggc cgttcgcggc cgaggggcgc agcccctggg
     8761 gggatgggag ctcggtgcct tgatgtgggc gccggcggtc gagtggcgac ggcgcggctt
     8821 gtccgcgccc tggtagattg cctggccgta ggccagccat ttttgagcgg ccagcggccg
     8881 cgataggccg acgcgaagcg gcggggcgta gggagcgcag cgaccgaagg gtaggcgctt
     8941 tttgcagctc ttcggctgtg cgctggccag acagttatgc acaggccagg cgggttttaa
     9001 gagttttaat aagttttaaa gagttttagg cggaaaaatc gccttttttc tcttttatat
     9061 cagtcactta catgtgtgac cggttcccaa tgtacggctt tgggttccca atgtacgggt
     9121 tccggttccc aatgtacggc tttgggttcc caatgtacgt gctatccaca ggaaagaagc
     9181 tttttcgacc tttttcccct gctagggcaa tttgccctag catctgctcc gtacattagg
     9241 aaccggcgga tgcttcgccc tcgatcaggt tgcggtagcg catgactagg atcgggccag
     9301 cctgccccgc ctcctccttc aaatcgtact ccggcaggtc atttgacccg atcagcttgc
     9361 gcacggtgaa acagaacttc ttgaactctc cggcgctgcc actgcgttcg tagatcgtct
     9421 tgaacaacca tctggcttct gccttgcctg cggcgcggcg tgccaggcgg tagagaaaac
     9481 ggccgatgcc gggatcgatc aaaaagtaat cggggtgaac cgtcagcacg tccgggttct
     9541 tgccttctgt gatctcgcgg tacatccaat cagctagctc gatctcgatg tactccggcc
     9601 gcccggtttc gctctttacg atcttgtagc ggctaatcaa ggcttcaccc tcggataccg
     9661 tcaccaggcg gccgttcttg gccttcttcg tacgctgcat ggcaacgtgc gtggtgttta
     9721 accgaatgca ggtttctacc aggtcgtctt tctgctttcc gccatcggct cgccggcaga
     9781 acttgagtac gtccgcaacg tgtggacgga acacgcggcc gggcttgtct cccttccctt
     9841 cccggtatcg gttcatggat tcggttagat gggaaaccgc catcagtacc aggtcgtaat
     9901 cccacacact ggccatgccg gccggccctg cggaaacctc tacgtgcccg tctggaagct
     9961 cgtagcggat cacctcgcca gctcgtcggt cacgcttcga cagacggaaa acggccacgt
    10021 ccatgatgct gcgactatcg cgggtgccca cgtcatagag catcggaacg aaaaaatctg
    10081 gttgctcgtc gcccttgggc ggcttcctaa tcgacgacgc accggctgcc ggcg
//
