LOCUS       sequence-378001-       10011 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains stuffer sequence into which sgRNAs can be cloned and 
            expresses mCherry from hepatocyte-specific promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10011)
  AUTHORS   Keys HR, Knouse KA
  TITLE     Genome-scale CRISPR screening in a single mouse liver.
  JOURNAL   Cell Genom. 2022 Dec 14;2(12):100217. doi: 
            10.1016/j.xgen.2022.100217. Epub 2022 Nov 15.
  PUBMED    36643909
REFERENCE   2  (bases 1 to 10011)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Genom."; date: "2022-12-14"; volume: "2(12):100217. doi"; pages: " 
            10.1016/j.xgen.2022.100217. Epub 2022 Nov 15"
COMMENT     Sequence Label: pLentiCRISPRv2-Stuffer-HepmCherry
FEATURES             Location/Qualifiers
     source          1..10011
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      513..522
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             519..1229
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(664..682)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     932..951
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    1248..1836
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1301..1321)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1719..1730)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             1908..2141
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(2167..2184)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2173..2397
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2443..2871
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2530..2549)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2740..2761
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2880..2900)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2885..3214
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3065..3200
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3127..3146
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        3262..3309
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3328..3702
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    3832..3953
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3869..3888)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3923..3942
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4002..4018)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4002..4018)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4015..4037)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4026..4042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4050..4056,4057..4074,4075..4080))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4095..4116
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4233..4250)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4404..4992)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4484..4503)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5163..5954,5955..6023))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5786..5805
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6024..6128)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6203..6222)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        6394..6773
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6775..6973
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6928..6948
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             6991..7171
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7218..7343
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7836..8069
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8254..8298
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8596..8713
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        8764..9012
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     8764..8784
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     8935..8954
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 aattttagta agtcttgtcc tggcatatta tacattccat cgatgtagtt cttgctcttt
       61 tttgtacgtc tctgtttaag agctatgctg gaaacagcat agcaagttta aataaggcta
      121 gtccgttatc aacttgaaaa agtggcaccg agtcggtgct tttttgaatt cgctagcacc
      181 ggcgcgccgg gggaggctgc tggtgaatat taaccaaggt caccccagtt atcggaggag
      241 caaacagggg ctaagtccac acgcgtggta ccgtctgtct gcacatttcg tagagcgagt
      301 gttccgatac tctaatctcc ctaggcaagg ttcatatttg tgtaggttac ttattctcct
      361 tttgttgact aagtcaataa tcagaatcag caggtttgga gtcagcttgg cagggatcag
      421 cagcctgggt tggaaggagg gggtataaaa gccccttcac caggagaagc cgtcacacag
      481 atccacaagc tcctgaccgg ttctagagcg ctgccaccat ggtgagcaag ggcgaggagg
      541 ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga
      601 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
      661 ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc
      721 ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact
      781 tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
      841 gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga
      901 agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct
      961 gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc
     1021 agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg
     1081 ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct
     1141 cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca
     1201 ccggcggcat ggacgagctg tacaagtaat gaacgcgtta agtcgacaat caacctctgg
     1261 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     1321 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     1381 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     1441 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     1501 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     1561 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     1621 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
     1681 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     1741 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     1801 agacgagtcg gatctccctt tgggccgcct ccccgcgtcg actttaagac caatgactta
     1861 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     1921 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
     1981 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     2041 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     2101 atccctcaga cccttttagt cagtgtggaa aatctctagc agggcccgtt taaacccgct
     2161 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
     2221 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
     2281 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
     2341 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt
     2401 ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg
     2461 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     2521 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     2581 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     2641 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     2701 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     2761 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     2821 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg
     2881 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     2941 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     3001 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     3061 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     3121 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
     3181 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat
     3241 tttcggatct gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta
     3301 taatacgaca aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt tccggtgctc
     3361 accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg
     3421 gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc
     3481 gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg
     3541 gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg
     3601 ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg
     3661 gccggcaact gcgtgcactt cgtggccgag gagcaggact gacacgtgct acgagatttc
     3721 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     3781 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt
     3841 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     3901 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     3961 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
     4021 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     4081 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     4141 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     4201 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     4261 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     4321 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     4381 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     4441 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     4501 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     4561 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     4621 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     4681 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     4741 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     4801 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     4861 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     4921 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     4981 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     5041 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     5101 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     5161 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     5221 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     5281 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     5341 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     5401 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     5461 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     5521 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     5581 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     5641 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     5701 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     5761 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     5821 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     5881 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     5941 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     6001 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     6061 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     6121 gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg
     6181 atcccctatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc
     6241 tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa
     6301 caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc
     6361 tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact agttattaat
     6421 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
     6481 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
     6541 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
     6601 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
     6661 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
     6721 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
     6781 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
     6841 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
     6901 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
     6961 tctatataag cagcgcgttt tgcctgtact gggtctctct ggttagacca gatctgagcc
     7021 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     7081 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     7141 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
     7201 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
     7261 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
     7321 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
     7381 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
     7441 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
     7501 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
     7561 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
     7621 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
     7681 cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta
     7741 tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga
     7801 agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg
     7861 ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa
     7921 ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag
     7981 catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg
     8041 gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt
     8101 tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg
     8161 aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac
     8221 tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta
     8281 gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa
     8341 ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct
     8401 atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc
     8461 ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac
     8521 agatccattc gattagtgaa cggatcggca ctgcgtgcgc caattctgca gacaaatggc
     8581 agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag
     8641 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa
     8701 aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttggt taattaaggt
     8761 accgagggcc tatttcccat gattccttca tatttgcata tacgatacaa ggctgttaga
     8821 gagataatta gaattaattt gactgtaaac acaaagatat tagtacaaaa tacgtgacgt
     8881 agaaagtaat aatttcttgg gtagtttgca gttttaaaat tatgttttaa aatggactat
     8941 catatgctta ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa
     9001 ggacgaaaca ccggagacgg ttgtaaatga gcacacaaaa tacacatgct aaaatattat
     9061 attctatgac ctttataaaa tcaaccaaaa tcttcttttt aataacttta gtatcaataa
     9121 ttagaatttt tatgttcctt tttgcaaact tttaataaaa atgagcaaaa taaaaaaacg
     9181 ctagttttag taactcgcgt tgttttcttc acctttaata atagctactc caccacttgt
     9241 tcctaagcgg tcagctcctg cttcaatcat tttttgagca tcttcaaatg ttctaactcc
     9301 accagctgct ttaactaaag cattgtcttt aacaactgac ttcattagtt taacatcttc
     9361 aaatgttgca cctgattttg aaaatcctgt tgatgtttta acaaattcta atccagcttc
     9421 aacagctatt tcacaagctt tcatgatttc ttcttttgtt aataaacaat tttccataat
     9481 acatttaaca acatgtgatc cagctgcttt ttttacagct ttcatgtctt ctaaaactaa
     9541 ttcataattt ttgtctttta atgcaccaat atttaatacc atatcaattt ctgttgcacc
     9601 atctttaatt gcttcagaaa cttcgaatgc ttttgtagct gttgtgcatg cacctagagg
     9661 aaaacctaca acatttgtta ttcctacatt tgtgcctttt aataattctt tacaatagct
     9721 tgttcaatat gaattaacac aaactgttgc aaaatcaaat tcaattgctt catcacataa
     9781 ttgtttaatt tcagctttcg tagcatcttg ttttaataat gtgtgatcta tatatttgtt
     9841 tagtttcatt ttttctccta tatattcatt tttaatttta attctttaat aatttcgtct
     9901 actttaactt tagcgttttg aacagattca ccaacaccta taaaataaat ttttagttta
     9961 ggttcagttc cacttgggcg aacagcaaat catgacttat cttctaaata a
//
